{"id":"https://openalex.org/W7066397227","doi":null,"title":"Identificaci\u00f3n pangen\u00f3mica de secuencias firma para la detecci\u00f3n de cepas del clado C-I de Clostridioides difficile mediante qPCR","display_name":"Identificaci\u00f3n pangen\u00f3mica de secuencias firma para la detecci\u00f3n de cepas del clado C-I de Clostridioides difficile mediante qPCR","publication_year":2021,"publication_date":"2021-01-01","ids":{"openalex":"https://openalex.org/W7066397227"},"language":"es","primary_location":{"id":"pmh:oai:kerwa.ucr.ac.cr:10669/83180","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/10669/83180","pdf_url":"https://www.kerwa.ucr.ac.cr/bitstreams/73d159e4-97e8-47ab-b9e1-73cf13d5b360/download","source":{"id":"https://openalex.org/S4306400069","display_name":"Investigative News in Education (Universidad de Costa Rica)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I198243066","host_organization_name":"Universidad Nacional","host_organization_lineage":["https://openalex.org/I198243066"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Universidad de Costa Rica, San Jos\u00e9, Costa Rica","raw_type":"tesis de maestr\u00eda"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.kerwa.ucr.ac.cr/bitstreams/73d159e4-97e8-47ab-b9e1-73cf13d5b360/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042727699","display_name":"Camilo Monge-Cascante","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Monge Cascante, Camilo","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5042727699"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T11288","display_name":"Clostridium difficile and Clostridium perfringens research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11288","display_name":"Clostridium difficile and Clostridium perfringens research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/clostridioides-difficile","display_name":"Clostridium difficile","score":0.996999979019165},{"id":"https://openalex.org/keywords/pan-genome","display_name":"pan-genome","score":0.6340000033378601},{"id":"https://openalex.org/keywords/signature-sequences","display_name":"signature sequences","score":0.8560000061988831},{"id":"https://openalex.org/keywords/qpcr-detection","display_name":"qPCR detection","score":0.6320000290870667},{"id":"https://openalex.org/keywords/molecular-diagnosis","display_name":"molecular diagnosis","score":0.6800000071525574},{"id":"https://openalex.org/keywords/clostridium-difficile-infection","display_name":"Clostridium difficile infection","score":0.9200000166893005},{"id":"https://openalex.org/keywords/toxinotyping","display_name":"toxinotyping","score":0.3230000138282776},{"id":"https://openalex.org/keywords/tcda","display_name":"TcdA","score":0.6579999923706055},{"id":"https://openalex.org/keywords/tcdb","display_name":"TcdB","score":0.9800000190734863},{"id":"https://openalex.org/keywords/draft-genome-sequence","display_name":"draft genome sequence","score":0.49300000071525574},{"id":"https://openalex.org/keywords/roary","display_name":"Roary","score":0.4740000069141388}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8463000059127808},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5724999904632568},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.28929999470710754},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1965000033378601},{"id":"https://openalex.org/C106337198","wikidata":"https://www.wikidata.org/wiki/Q13564012","display_name":"Illumina dye sequencing","level":4,"score":0.19599999487400055},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.1949000060558319},{"id":"https://openalex.org/C2776460866","wikidata":"https://www.wikidata.org/wiki/Q170065","display_name":"Pathogen","level":2,"score":0.17499999701976776},{"id":"https://openalex.org/C2910067853","wikidata":"https://www.wikidata.org/wiki/Q27438828","display_name":"Clostridioides","level":2,"score":0.17479999363422394},{"id":"https://openalex.org/C15708023","wikidata":"https://www.wikidata.org/wiki/Q80083","display_name":"Humanities","level":1,"score":0.17249999940395355},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.16220000386238098}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:kerwa.ucr.ac.cr:10669/83180","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/10669/83180","pdf_url":"https://www.kerwa.ucr.ac.cr/bitstreams/73d159e4-97e8-47ab-b9e1-73cf13d5b360/download","source":{"id":"https://openalex.org/S4306400069","display_name":"Investigative News in Education (Universidad de Costa Rica)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I198243066","host_organization_name":"Universidad Nacional","host_organization_lineage":["https://openalex.org/I198243066"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Universidad de Costa Rica, San Jos\u00e9, Costa Rica","raw_type":"tesis de maestr\u00eda"},{"id":"pmh:oai:www.kerwa.ucr.ac.cr:10669/83180","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10669/83180","pdf_url":null,"source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Universidad de Costa Rica, San Jos\u00e9, Costa Rica","raw_type":"info:eu-repo/semantics/masterThesis"}],"best_oa_location":{"id":"pmh:oai:kerwa.ucr.ac.cr:10669/83180","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/10669/83180","pdf_url":"https://www.kerwa.ucr.ac.cr/bitstreams/73d159e4-97e8-47ab-b9e1-73cf13d5b360/download","source":{"id":"https://openalex.org/S4306400069","display_name":"Investigative News in Education (Universidad de Costa Rica)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I198243066","host_organization_name":"Universidad Nacional","host_organization_lineage":["https://openalex.org/I198243066"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Universidad de Costa Rica, San Jos\u00e9, Costa Rica","raw_type":"tesis de maestr\u00eda"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8560000061988831}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7066397227.pdf","grobid_xml":"https://content.openalex.org/works/W7066397227.grobid-xml"},"referenced_works_count":107,"referenced_works":["https://openalex.org/W102715436","https://openalex.org/W1510123427","https://openalex.org/W1886308172","https://openalex.org/W1916481354","https://openalex.org/W1958058086","https://openalex.org/W1977141047","https://openalex.org/W1978450852","https://openalex.org/W1982411556","https://openalex.org/W2015737379","https://openalex.org/W2027207787","https://openalex.org/W2029660620","https://openalex.org/W2032019813","https://openalex.org/W2037729031","https://openalex.org/W2040322093","https://openalex.org/W2055615325","https://openalex.org/W2071874661","https://openalex.org/W2073288136","https://openalex.org/W2078048409","https://openalex.org/W2088998577","https://openalex.org/W2089270461","https://openalex.org/W2089730047","https://openalex.org/W2096093282","https://openalex.org/W2099877833","https://openalex.org/W2101233913","https://openalex.org/W2102418148","https://openalex.org/W2104491546","https://openalex.org/W2104677379","https://openalex.org/W2106763286","https://openalex.org/W2106831385","https://openalex.org/W2116041602","https://openalex.org/W2116671076","https://openalex.org/W2117012676","https://openalex.org/W2120902911","https://openalex.org/W2122673596","https://openalex.org/W2123087277","https://openalex.org/W2131340296","https://openalex.org/W2133790733","https://openalex.org/W2134819521","https://openalex.org/W2141951202","https://openalex.org/W2143373317","https://openalex.org/W2144836423","https://openalex.org/W2148071366","https://openalex.org/W2149531726","https://openalex.org/W2161990844","https://openalex.org/W2165833212","https://openalex.org/W2166374078","https://openalex.org/W2166432938","https://openalex.org/W2167805911","https://openalex.org/W2171602727","https://openalex.org/W2207403739","https://openalex.org/W2259324737","https://openalex.org/W2267940328","https://openalex.org/W2340958225","https://openalex.org/W2344227387","https://openalex.org/W2345528828","https://openalex.org/W2346636740","https://openalex.org/W2347869237","https://openalex.org/W2357750051","https://openalex.org/W2401044874","https://openalex.org/W2419335772","https://openalex.org/W2461622101","https://openalex.org/W2464112039","https://openalex.org/W2511449838","https://openalex.org/W2531684910","https://openalex.org/W2549760434","https://openalex.org/W2554414896","https://openalex.org/W2566975142","https://openalex.org/W2568845238","https://openalex.org/W2579989238","https://openalex.org/W2584810127","https://openalex.org/W2593187224","https://openalex.org/W2594064363","https://openalex.org/W2623452143","https://openalex.org/W2732954316","https://openalex.org/W2736558481","https://openalex.org/W2773151689","https://openalex.org/W2784407026","https://openalex.org/W2786138785","https://openalex.org/W2787903069","https://openalex.org/W2789348707","https://openalex.org/W2791326569","https://openalex.org/W2792237904","https://openalex.org/W2793576980","https://openalex.org/W2793598378","https://openalex.org/W2804160113","https://openalex.org/W2804906140","https://openalex.org/W2810213850","https://openalex.org/W2886978368","https://openalex.org/W2889208110","https://openalex.org/W2890074483","https://openalex.org/W2902872341","https://openalex.org/W2908796034","https://openalex.org/W2939215469","https://openalex.org/W2943667402","https://openalex.org/W2949775143","https://openalex.org/W2950417030","https://openalex.org/W2951254987","https://openalex.org/W2951584314","https://openalex.org/W2952473089","https://openalex.org/W2961485256","https://openalex.org/W2968050891","https://openalex.org/W2997808962","https://openalex.org/W3025928348","https://openalex.org/W3036189339","https://openalex.org/W3045244624","https://openalex.org/W3045957397","https://openalex.org/W3171901253"],"related_works":["https://openalex.org/W4401872578","https://openalex.org/W2095002008","https://openalex.org/W1588311531","https://openalex.org/W2890074483","https://openalex.org/W7008663519","https://openalex.org/W1968266921","https://openalex.org/W2502925740","https://openalex.org/W4405279902","https://openalex.org/W7097145149","https://openalex.org/W4403292838"],"abstract_inverted_index":{"Clostridioides":[0],"difficile":[1,40,204,382,588],"es":[2,158],"un":[3,102,188,576],"importante":[4],"pat\u00f3geno":[5],"humano.":[6],"Las":[7],"cepas":[8,36,135,175,201,209,213,335,369,435,463,585,615,640],"de":[9,38,58,67,94,96,104,115,117,134,144,151,154,174,190,200,202,208,212,214,237,239,253,264,290,318,349,352,359,368,374,380,387,408,410,454,461,473,477,495,498,502,509,514,518,534,538,543,548,555,559,571,582,584,586,597,602,608,636,638,647],"esta":[10],"bacteria":[11],"han":[12,112],"sido":[13],"organizadas":[14],"en":[15,47,148,187,195,249,281,316,333,342,400,492,506,540,550,561,595,641],"cinco":[16],"clados":[17],"comunes,":[18],"numerados":[19],"del":[20,137,164,176,307,336,370,383,436,589,616],"1":[21],"al":[22,445],"5,":[23],"y":[24,31,63,79,92,107,126,130,160,224,244,251,256,260,269,324,373,386,394,420,468,504,517,536,553,557,628,643,649],"tres":[25,59,234,606],"divergentes,":[26],"conocidos":[27],"como":[28,45,459],"C-I,":[29,210,338,591],"C-II":[30],"C-III.":[32],"La":[33],"infecci\u00f3n":[34],"con":[35,89,278,363,378,430,511,521,545,564,567,600,645],"patog\u00e9nicas":[37],"C.":[39,203,357,381,587],"(CDI)":[41],"se":[42,70,111,232,270,404,574],"manifiesta":[43],"cl\u00ednicamente":[44],"diarrea":[46],"el":[48,56,167,196,329,406,421,424,525,529],"paciente.":[49],"Estos":[50,322,610],"efectos":[51],"son":[52,83,127],"mediados":[53],"principalmente":[54],"por":[55,183,397,465,626],"efecto":[57],"toxinas":[60,77],"(TcdA,":[61],"TcdB":[62],"CDT).":[64],"El":[65,427],"diagn\u00f3stico":[66,145,191],"las":[68,76,228,294,334,392,416,434,462,614],"CDI":[69],"realiza":[71],"usualmente":[72],"mediante":[73],"inmunoensayos":[74],"para":[75,185,287,293,328,356,415,423,449,475,485,528,578],"A":[78,569],"B,":[80],"los":[81,142,152,217,298,398,411,481,488,496,507,541,562,630],"cuales":[82,218,299,482,489],"r\u00e1pidos,":[84],"pero":[85],"poco":[86],"sensibles,":[87],"o":[88,593],"cultivo":[90,642],"toxig\u00e9nico":[91],"ensayos":[93,114,317,508,542],"neutralizaci\u00f3n":[95],"citotoxicidad":[97],"celular,":[98],"ambos":[99],"demandantes":[100],"desde":[101],"punto":[103,320],"vista":[105],"t\u00e9cnico":[106],"econ\u00f3mico.":[108],"Como":[109],"alternativa,":[110],"desarrollado":[113],"amplificaci\u00f3n":[116],"\u00e1cidos":[118],"nucleicos,":[119],"dado":[120],"que":[121,140,274,433,442,524,613,629],"tienen":[122],"alta":[123,276],"sensibilidad,":[124],"especificidad,":[125],"relativamente":[128],"r\u00e1pidos":[129],"accesibles.":[131],"Existen":[132],"reportes":[133],"toxig\u00e9nicas":[136,592],"Clado":[138,177,337,371,384,437,590,617],"C-I":[139,178,372,385,438,464,618],"escapan":[141],"m\u00e9todos":[143,266],"utilizados":[146],"rutinariamente":[147],"la":[149,162,450,580,634],"mayor\u00eda":[150],"centros":[153],"salud.":[155],"Esta":[156],"situaci\u00f3n":[157],"preocupante,":[159],"motiv\u00f3":[161],"realizaci\u00f3n":[163],"presente":[165,332],"proyecto,":[166],"cual":[168],"pretend\u00eda":[169],"identificar":[170,227],"secuencias":[171,230,273,295,417,455,479,620],"firma":[172,231,296,418,621],"nucleot\u00eddicas":[173],"susceptibles":[179,622],"a":[180,347,366,623],"ser":[181,624],"amplificadas":[182,625],"qPCR,":[184,627],"emplearlas":[186],"m\u00e9todo":[189],"molecular.":[192],"Se":[193,284,390,470],"incluyeron":[194],"an\u00e1lisis":[197,257,428],"77":[198],"genomas":[199],"calidad":[205,223],"draft":[206],"(10":[207],"67":[211],"Clados":[215],"1-5)":[216],"fueron":[219,267,300,313,339,457,483,490],"ensamblados,":[220],"verificada":[221],"su":[222],"anotados.":[225],"Para":[226],"posibles":[229],"utilizaron":[233],"t\u00e9cnicas:":[235],"estudios":[236],"asociaci\u00f3n":[238],"genoma":[240,446],"completo":[241],"(DBGWAS,":[242],"TreeWAS":[243],"Roary/Scoary),":[245],"procesos":[246],"automatizados":[247],"basados":[248],"referencia":[250],"pareo":[252],"k-mer":[254],"(Neptune)":[255],"pangen\u00f3micos":[258],"(Roary":[259],"Anvi\u2019o).":[261],"Los":[262,310],"resultados":[263,573],"estos":[265,572,605],"consolidados,":[268],"eliminaron":[271],"aquellas":[272],"tuvieran":[275],"identidad":[277],"otras":[279],"presentes":[280],"otros":[282,388],"microorganismos.":[283],"utiliz\u00f3":[285],"Primer-BLAST":[286],"dise\u00f1ar":[288],"juegos":[289],"oligonucle\u00f3tidos":[291],"cebadores":[292,399,414,474,499,631],"identificadas,":[297],"depurados":[301],"seg\u00fan":[302],"contenido":[303],"GC,":[304],"autocomplementariedad,":[305],"largo":[306],"producto,":[308],"Tm.":[309],"mejores":[311,413],"pares":[312,472,497,607],"probados":[314,340,491],"inicialmente":[315],"PCR":[319],"final.":[321],"cebadores,":[323],"uno":[325],"adicional":[326],"dise\u00f1ado":[327,422,527],"gen":[330,425,530],"tcdB":[331,531],"adicionalmente":[341],"qPCR":[343,510,544],"usando":[344],"ADN":[345,364,512,546,583,637],"extra\u00eddo":[346,365,513,547],"partir":[348,367,570],"cultivos":[350,379,515,549],"bacterianos,":[351],"heces":[353,360,375,519,565,596,644],"humanas":[354,361,376],"negativas":[355],"difficile,":[358],"inoculadas":[362,377,520,566],"clados.":[389],"calcularon":[391],"sensibilidades":[393],"especificidades":[395],"demostradas":[396],"cada":[401],"ensayo.":[402],"Finalmente,":[403],"aproxim\u00f3":[405],"l\u00edmite":[407],"detecci\u00f3n":[409],"dos":[412],"identificadas":[419],"tcdB.":[426],"pangen\u00f3mico":[429],"Roary":[431],"revel\u00f3":[432],"comparten":[439],"890":[440],"CDSs":[441],"no":[443],"pertenecen":[444],"core":[447],"determinado":[448],"especie.":[451],"Once":[452],"conglomerados":[453],"codificantes":[456],"identificados":[458],"exclusivos":[460],"Anvi\u2019o,":[466],"Roary/Scoary":[467],"Treewas.":[469],"generaron":[471],"ocho":[476],"estas":[478],"firma,":[480],"depurados,":[484],"sintetizar":[486],"cuatro,":[487],"laboratorio.":[493],"Dos":[494],"mostraron":[500],"100%":[501,533,558,646],"sensibilidad":[503,535,556],"especificidad":[505,539,560,648],"l\u00edquidos":[516],"cultivos,":[522],"mientras":[523],"par":[526],"mostr\u00f3":[532],"72.7%":[537],"medio":[551],"l\u00edquido,":[552],"66%":[554],"realizados":[563],"cultivos.":[568],"propuso":[575],"algoritmo":[577],"determinar":[579],"presencia":[581,635],"no,":[594],"pacientes":[598],"diarreicos":[599],"sospecha":[601],"CDI,":[603],"utilizando":[604],"cebadores.":[609],"hallazgos":[611],"confirman":[612],"presentan":[619],"dise\u00f1ados":[632],"detectan":[633],"dichas":[639],"sensibilidad.":[650]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2025-10-10T00:00:00"}
