{"id":"https://openalex.org/W70495492","doi":null,"title":"Analysis of protein phosphorylation using mass spectrometry.","display_name":"Analysis of protein phosphorylation using mass spectrometry.","publication_year":2008,"publication_date":"2008-05-01","ids":{"openalex":"https://openalex.org/W70495492","mag":"70495492","pmid":"https://pubmed.ncbi.nlm.nih.gov/18782944"},"language":"en","primary_location":{"id":"pmid:18782944","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18782944","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chang Gung medical journal","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112507571","display_name":"Hsin-Yi Wu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I91807558","display_name":"National Cheng Kung University","ror":"https://ror.org/01b8kcc49","country_code":"TW","type":"education","lineage":["https://openalex.org/I91807558"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Hsin-Yi Wu","raw_affiliation_strings":["Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC","institution_ids":["https://openalex.org/I91807558"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047171658","display_name":"Pao\u2010Chi Liao","orcid":"https://orcid.org/0000-0001-8510-9870","observed_orcids":["https://orcid.org/0000-0001-8510-9870"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Pao-Chi Liao","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1245,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.36712355,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"31","issue":"3","first_page":"217","last_page":"27"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-phosphorylation","display_name":"protein phosphorylation","score":1.0},{"id":"https://openalex.org/keywords/phosphopeptide-enrichment","display_name":"phosphopeptide enrichment","score":0.8019999861717224},{"id":"https://openalex.org/keywords/immobilized-metal-affinity-chromatography","display_name":"immobilized metal affinity chromatography","score":0.9279999732971191},{"id":"https://openalex.org/keywords/phosphatase-treatment","display_name":"phosphatase treatment","score":0.8100000023841858},{"id":"https://openalex.org/keywords/neutral-loss-scan","display_name":"neutral loss scan","score":0.9459999799728394},{"id":"https://openalex.org/keywords/precursor-ion-scan","display_name":"precursor ion scan","score":0.8859999775886536},{"id":"https://openalex.org/keywords/isotopic-labeling","display_name":"isotopic labeling","score":0.8700000047683716},{"id":"https://openalex.org/keywords/mass-tags","display_name":"mass tags","score":0.9470000267028809},{"id":"https://openalex.org/keywords/quantitative-phosphoproteomics","display_name":"quantitative phosphoproteomics","score":0.8980000019073486},{"id":"https://openalex.org/keywords/signaling-networks","display_name":"signaling networks","score":0.6119999885559082}],"concepts":[{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.8353466987609863},{"id":"https://openalex.org/C2779933727","wikidata":"https://www.wikidata.org/wiki/Q7187535","display_name":"Phosphopeptide","level":3,"score":0.8119336366653442},{"id":"https://openalex.org/C87325107","wikidata":"https://www.wikidata.org/wiki/Q7251493","display_name":"Protein phosphorylation","level":4,"score":0.7619237303733826},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.635535717010498},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6095407009124756},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.502886950969696},{"id":"https://openalex.org/C6675166","wikidata":"https://www.wikidata.org/wiki/Q7187538","display_name":"Phosphoproteomics","level":5,"score":0.4713052213191986},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.343996524810791},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.31989210844039917},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2844228446483612},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.23496109247207642},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.21348461508750916},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:18782944","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18782944","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chang Gung medical journal","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.533.2573","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.533.2573","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://memo.cgu.edu.tw/cgmj/3103/310301.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W1555247363","https://openalex.org/W1575714113","https://openalex.org/W1577909840","https://openalex.org/W1966665800","https://openalex.org/W1973969209","https://openalex.org/W1974842041","https://openalex.org/W1975938671","https://openalex.org/W1984646706","https://openalex.org/W1984871500","https://openalex.org/W1985771129","https://openalex.org/W1996369227","https://openalex.org/W1996693787","https://openalex.org/W1997835441","https://openalex.org/W2000207287","https://openalex.org/W2003019832","https://openalex.org/W2004704197","https://openalex.org/W2009682799","https://openalex.org/W2009906189","https://openalex.org/W2012611489","https://openalex.org/W2013748646","https://openalex.org/W2018072960","https://openalex.org/W2020208200","https://openalex.org/W2027387754","https://openalex.org/W2030157681","https://openalex.org/W2033199777","https://openalex.org/W2033889320","https://openalex.org/W2039690045","https://openalex.org/W2041162439","https://openalex.org/W2042629507","https://openalex.org/W2046461084","https://openalex.org/W2047007872","https://openalex.org/W2050591755","https://openalex.org/W2053021973","https://openalex.org/W2058232238","https://openalex.org/W2059964424","https://openalex.org/W2069698030","https://openalex.org/W2070066745","https://openalex.org/W2075045273","https://openalex.org/W2089144395","https://openalex.org/W2090804458","https://openalex.org/W2099255819","https://openalex.org/W2101749564","https://openalex.org/W2110119321","https://openalex.org/W2118127847","https://openalex.org/W2128987420","https://openalex.org/W2141376777","https://openalex.org/W2165923048"],"related_works":["https://openalex.org/W2380559491","https://openalex.org/W2046461084","https://openalex.org/W1974842041","https://openalex.org/W2387788458","https://openalex.org/W2389474430","https://openalex.org/W2024620187","https://openalex.org/W2325605624","https://openalex.org/W2122972590","https://openalex.org/W2165923048","https://openalex.org/W2087084848"],"abstract_inverted_index":{"Protein":[0],"phosphorylation":[1,151,164,191],"has":[2],"been":[3,158],"known":[4],"to":[5,34,56,127],"be":[6,32],"a":[7,166,184],"pivotal":[8],"modification":[9],"regulating":[10],"many":[11],"cellular":[12],"activities":[13],"and":[14,72,118,133,146,176],"functions.":[15],"Except":[16],"for":[17,50,77,144,187],"several":[18],"conventional":[19],"techniques,":[20],"mass":[21,47,119],"spectrometry-based":[22,48],"strategies":[23],"are":[24,75,90,121],"increasingly":[25],"considered":[26],"as":[27,65],"vital":[28],"tools":[29],"that":[30,154],"can":[31,179],"utilized":[33],"characterize":[35],"phosphorylated":[36],"peptides":[37],"or":[38,100],"proteins.":[39,194],"In":[40,111],"this":[41],"article,":[42],"we":[43,178],"summarized":[44],"currently":[45],"available":[46],"techniques":[49,89],"the":[51,57,83,87,105,108,150,170,181,188],"analysis":[52,125,161,189],"of":[53,60,86,92,107,130,136,149,162,174,183,190,193],"phosphorylation.":[54],"Due":[55],"low":[58],"abundance":[59],"phosphopeptides,":[61],"enrichment":[62,88],"steps":[63],"such":[64],"specific":[66,73],"antibodies,":[67],"immobilized":[68],"metal":[69],"affinity":[70],"chromatography,":[71],"tags":[74,120],"crucial":[76],"their":[78],"use":[79],"in":[80],"detection.":[81],"Since":[82],"non-specific":[84],"binding":[85],"constantly":[91],"major":[93],"concerns,":[94],"phosphatase":[95],"treatment,":[96],"neutral":[97],"loss":[98],"scan,":[99],"precursor":[101],"ion":[102],"scan":[103],"enable":[104],"recognition":[106],"phosphopeptide":[109],"signals.":[110],"addition,":[112],"quantitative":[113],"methods":[114,143],"including":[115],"isotope":[116],"labeling":[117],"also":[122],"discussed.":[123],"Phosphoproteome":[124],"seems":[126],"provide":[128],"elucidation":[129],"signaling":[131],"networks":[132],"global":[134],"decipherment":[135],"cell":[137],"activities,":[138],"which":[139],"require":[140],"powerful":[141],"analytical":[142],"complete":[145],"routine":[147],"identification":[148],"event.":[152],"Despite":[153],"numerous":[155],"approaches":[156],"have":[157],"exploited,":[159],"comprehensive":[160,185],"protein":[163],"remains":[165],"challenging":[167],"task.":[168],"With":[169],"progressively":[171],"more":[172],"improvements":[173],"instruments":[175],"methodologies,":[177],"foresee":[180],"implementation":[182],"approach":[186],"states":[192]},"counts_by_year":[{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}