{"id":"https://openalex.org/W7046777658","doi":null,"title":"Developing an optimized PCR protocol for microsatellite analysis in Vaccinium myrtillus","display_name":"Developing an optimized PCR protocol for microsatellite analysis in Vaccinium myrtillus","publication_year":2024,"publication_date":"2024-01-01","ids":{"openalex":"https://openalex.org/W7046777658"},"language":"en","primary_location":{"id":"pmh:oai:DiVA.org:liu-205778","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-205778","pdf_url":"https://liu.diva-portal.org/smash/get/diva2:1881235/FULLTEXT01","source":{"id":"https://openalex.org/S4306400838","display_name":"DiVA (Linkoping University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I102134673","host_organization_name":"Link\u00f6ping University","host_organization_lineage":["https://openalex.org/I102134673"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/bachelorThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://liu.diva-portal.org/smash/get/diva2:1881235/FULLTEXT01","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Wreth, Cajsa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Wreth, Cajsa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.9746000170707703,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.9746000170707703,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11468","display_name":"Genetic Mapping and Diversity in Plants and Animals","score":0.012600000016391277,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12605","display_name":"Berry genetics and cultivation research","score":0.012000000104308128,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/vaccinium-myrtillus","display_name":"Vaccinium myrtillus","score":0.9900000095367432},{"id":"https://openalex.org/keywords/microsatellite-markers","display_name":"microsatellite markers","score":0.9710000157356262},{"id":"https://openalex.org/keywords/pcr-optimization","display_name":"PCR optimization","score":0.8769999742507935},{"id":"https://openalex.org/keywords/annealing-temperature","display_name":"annealing temperature","score":0.9660000205039978},{"id":"https://openalex.org/keywords/genetic-diversity","display_name":"genetic diversity","score":0.9800000190734863},{"id":"https://openalex.org/keywords/crop-wild-relatives","display_name":"crop wild relatives","score":0.9739999771118164},{"id":"https://openalex.org/keywords/genetic-conservation","display_name":"genetic conservation","score":0.5040000081062317},{"id":"https://openalex.org/keywords/primer-dimers","display_name":"primer dimers","score":0.7049999833106995}],"concepts":[{"id":"https://openalex.org/C81977670","wikidata":"https://www.wikidata.org/wiki/Q585259","display_name":"Genetic diversity","level":3,"score":0.7768999934196472},{"id":"https://openalex.org/C61320498","wikidata":"https://www.wikidata.org/wiki/Q265193","display_name":"Microsatellite","level":4,"score":0.7731000185012817},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6643999814987183},{"id":"https://openalex.org/C2776762870","wikidata":"https://www.wikidata.org/wiki/Q5079636","display_name":"Vaccinium","level":2,"score":0.5612000226974487},{"id":"https://openalex.org/C130217890","wikidata":"https://www.wikidata.org/wiki/Q47041","display_name":"Biodiversity","level":2,"score":0.5386999845504761},{"id":"https://openalex.org/C134387633","wikidata":"https://www.wikidata.org/wiki/Q11395","display_name":"Domestication","level":2,"score":0.5103999972343445},{"id":"https://openalex.org/C137580998","wikidata":"https://www.wikidata.org/wiki/Q235352","display_name":"Crop","level":2,"score":0.5102999806404114},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.48910000920295715},{"id":"https://openalex.org/C2775908993","wikidata":"https://www.wikidata.org/wiki/Q60708120","display_name":"Bilberry","level":2,"score":0.4388999938964844},{"id":"https://openalex.org/C190789776","wikidata":"https://www.wikidata.org/wiki/Q767511","display_name":"Genetic marker","level":3,"score":0.38690000772476196},{"id":"https://openalex.org/C68873052","wikidata":"https://www.wikidata.org/wiki/Q349856","display_name":"Genetic variation","level":3,"score":0.37790000438690186},{"id":"https://openalex.org/C2776993194","wikidata":"https://www.wikidata.org/wiki/Q5413585","display_name":"Vaccinium myrtillus","level":2,"score":0.37770000100135803},{"id":"https://openalex.org/C194187813","wikidata":"https://www.wikidata.org/wiki/Q3073371","display_name":"Genetic erosion","level":4,"score":0.3776000142097473},{"id":"https://openalex.org/C54286561","wikidata":"https://www.wikidata.org/wiki/Q397350","display_name":"Agroforestry","level":1,"score":0.37540000677108765},{"id":"https://openalex.org/C1924315","wikidata":"https://www.wikidata.org/wiki/Q5187949","display_name":"Crop diversity","level":3,"score":0.36910000443458557},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.3668000102043152},{"id":"https://openalex.org/C549605437","wikidata":"https://www.wikidata.org/wiki/Q1229911","display_name":"Food security","level":3,"score":0.3190999925136566},{"id":"https://openalex.org/C118518473","wikidata":"https://www.wikidata.org/wiki/Q11451","display_name":"Agriculture","level":2,"score":0.3089999854564667},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.27469998598098755},{"id":"https://openalex.org/C46576788","wikidata":"https://www.wikidata.org/wiki/Q5532925","display_name":"Genetic structure","level":4,"score":0.2703999876976013},{"id":"https://openalex.org/C172777353","wikidata":"https://www.wikidata.org/wiki/Q1374271","display_name":"Genetic variability","level":4,"score":0.25609999895095825}],"mesh":[],"locations_count":1,"locations":[{"id":"pmh:oai:DiVA.org:liu-205778","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-205778","pdf_url":"https://liu.diva-portal.org/smash/get/diva2:1881235/FULLTEXT01","source":{"id":"https://openalex.org/S4306400838","display_name":"DiVA (Linkoping University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I102134673","host_organization_name":"Link\u00f6ping University","host_organization_lineage":["https://openalex.org/I102134673"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/bachelorThesis"}],"best_oa_location":{"id":"pmh:oai:DiVA.org:liu-205778","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-205778","pdf_url":"https://liu.diva-portal.org/smash/get/diva2:1881235/FULLTEXT01","source":{"id":"https://openalex.org/S4306400838","display_name":"DiVA (Linkoping University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I102134673","host_organization_name":"Link\u00f6ping University","host_organization_lineage":["https://openalex.org/I102134673"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/bachelorThesis"},"sustainable_development_goals":[{"score":0.958299994468689,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7046777658.pdf","grobid_xml":"https://content.openalex.org/works/W7046777658.grobid-xml"},"referenced_works_count":21,"referenced_works":["https://openalex.org/W8742182","https://openalex.org/W1771373876","https://openalex.org/W1992128517","https://openalex.org/W2046747761","https://openalex.org/W2082983676","https://openalex.org/W2095578120","https://openalex.org/W2102222986","https://openalex.org/W2157641482","https://openalex.org/W2171787842","https://openalex.org/W2188300929","https://openalex.org/W2220131908","https://openalex.org/W2961887966","https://openalex.org/W3045531312","https://openalex.org/W3049522820","https://openalex.org/W3134880137","https://openalex.org/W3186111258","https://openalex.org/W3201222371","https://openalex.org/W4297968758","https://openalex.org/W4385123314","https://openalex.org/W4386996484","https://openalex.org/W4388768015"],"related_works":["https://openalex.org/W6995583300","https://openalex.org/W2150765324","https://openalex.org/W7051653726","https://openalex.org/W2594070469","https://openalex.org/W1771373876","https://openalex.org/W2188663727","https://openalex.org/W2590667641","https://openalex.org/W6939290487","https://openalex.org/W807965015","https://openalex.org/W4405375506"],"abstract_inverted_index":{"In":[0,101,201],"the":[1,92,131,142,174,184,191,205,214],"future,":[2],"food":[3,59],"security":[4],"will":[5],"face":[6],"significant":[7],"challenges":[8],"due":[9,160],"to":[10,20,26,47,57,96,161,186,203,218],"climate":[11],"change":[12],"and":[13,51,79,120,137,166],"a":[14,35,65],"growing":[15],"world":[16],"population.":[17],"One":[18],"approach":[19],"make":[21],"agriculture":[22],"more":[23,62,129],"sustainable":[24],"is":[25,72],"preserve":[27],"biodiversity":[28],"by":[29,196],"utilizing":[30],"crop":[31,41,83],"wild":[32,42],"relatives":[33,43],"as":[34],"source":[36],"of":[37,122,130,141,163,177,221],"genetic":[38,67,151,175,199,209],"material.":[39],"These":[40],"are":[44],"closely":[45],"related":[46],"today\u2019s":[48],"cultivated":[49],"crops":[50],"can":[52,89,216],"be":[53,97],"an":[54,73],"important":[55,74],"asset":[56],"combat":[58],"insecurity.":[60],"Gaining":[61],"knowledge":[63,207],"about":[64,208],"species\u2019":[66],"diversity":[68,152,176,210],"through":[69],"microsatellite":[70,93,105],"analysis":[71],"step":[75],"for":[76,99,109,117,149,224],"future":[77,150],"conservation":[78],"potential":[80],"utilization":[81],"in":[82,126,169,213],"improvement.":[84],"However,":[85],"before":[86],"these":[87],"studies":[88],"take":[90],"place":[91],"markers":[94,106,119,145],"have":[95],"optimized":[98,108],"PCR.":[100],"this":[102],"study,":[103],"eleven":[104,143],"were":[107,115,146],"bilberry":[110],"individuals.":[111,171],"Optimized":[112],"annealing":[113],"temperatures":[114],"found":[116],"all":[118],"most":[121],"them":[123],"had":[124],"amplification":[125],"three":[127],"or":[128],"individuals":[132],"tested":[133,144],"from":[134],"Sweden,":[135],"Finland":[136],"Iceland.":[138],"Ten":[139],"out":[140],"regarded":[147],"suitable":[148],"analyses.":[153],"The":[154],"eleventh,":[155],"VCB-C00694,":[156],"was":[157],"considered":[158],"unsuitable":[159],"formation":[162],"primer":[164],"dimers":[165],"not":[167],"amplifying":[168],"several":[170],"By":[172],"assessing":[173],"bilberry,":[178],"Vaccinium":[179,194],"myrtillus,":[180],"it":[181],"opens":[182],"up":[183],"possibility":[185],"enrich":[187],"their":[188,222],"domesticated":[189],"relative":[190],"American":[192],"blueberry,":[193],"corymbosum,":[195],"introducing":[197],"new":[198],"variety.":[200],"relation":[202],"this,":[204],"increased":[206],"among":[211],"bilberries":[212],"Nordic":[215],"lead":[217],"better":[219],"understanding":[220],"need":[223],"conservation.":[225]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2025-10-10T00:00:00"}