{"id":"https://openalex.org/W7036615708","doi":null,"title":"CLONING, EXPRESSION AND CHARACTERIZATION OF CUTINASE, A FUNGAL LIPOLYTIC ENZYME","display_name":"CLONING, EXPRESSION AND CHARACTERIZATION OF CUTINASE, A FUNGAL LIPOLYTIC ENZYME","publication_year":1991,"publication_date":"1991-01-01","ids":{"openalex":"https://openalex.org/W7036615708"},"language":"en","primary_location":{"id":"pmh:oai:repository.helmholtz-hzi.de:10033/623722","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10033/623722","pdf_url":"https://repository.helmholtz-hzi.de/bitstream/10033/623722/1/Lauwereys243.pdf","source":{"id":"https://openalex.org/S4377196474","display_name":"HZI (Helmholtz Centre for Infection Research)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210124929","host_organization_name":"Helmholtz Centre for Infection Research","host_organization_lineage":["https://openalex.org/I4210124929"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"conference paper"},"volume":null,"type":"conference-paper","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://repository.helmholtz-hzi.de/bitstream/10033/623722/1/Lauwereys243.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110110210","display_name":"Mark Lauwereys","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lauwereys, Mark","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090456467","display_name":"Pieter DE GEUS","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"de Geus, Pieter","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"De Meutter, J.","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"De Meutter, J.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Stanssens, P.","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Stanssens, P.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5016806475","display_name":"Gaston E. Matthyssens","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Matthyssens, G.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.119,"has_fulltext":true,"cited_by_count":23,"citation_normalized_percentile":{"value":0.7826087,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.6869999766349792,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.6869999766349792,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.2863999903202057,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.006000000052154064,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cutinase","display_name":"cutinase","score":1.0},{"id":"https://openalex.org/keywords/fusarium-solani-pisi","display_name":"Fusarium solani pisi","score":0.9440000057220459},{"id":"https://openalex.org/keywords/lipolytic-enzymes","display_name":"lipolytic enzymes","score":0.7979999780654907},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.6840000152587891},{"id":"https://openalex.org/keywords/periplasmic-expression","display_name":"periplasmic expression","score":0.6990000009536743},{"id":"https://openalex.org/keywords/tac-promoter","display_name":"tac promoter","score":0.7229999899864197},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.7139999866485596},{"id":"https://openalex.org/keywords/tributyrin","display_name":"tributyrin","score":0.8859999775886536},{"id":"https://openalex.org/keywords/triolein","display_name":"triolein","score":0.9980000257492065},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.7540000081062317},{"id":"https://openalex.org/keywords/fungal-lipase","display_name":"fungal lipase","score":0.5130000114440918}],"concepts":[{"id":"https://openalex.org/C2777613533","wikidata":"https://www.wikidata.org/wiki/Q4273972","display_name":"Cutinase","level":3,"score":0.881600022315979},{"id":"https://openalex.org/C2778855143","wikidata":"https://www.wikidata.org/wiki/Q4116129","display_name":"Tributyrin","level":4,"score":0.8677999973297119},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.7251999974250793},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6840999722480774},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6431999802589417},{"id":"https://openalex.org/C201663137","wikidata":"https://www.wikidata.org/wiki/Q21198990","display_name":"Periplasmic space","level":4,"score":0.6011000275611877},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5318999886512756},{"id":"https://openalex.org/C2781157690","wikidata":"https://www.wikidata.org/wiki/Q413929","display_name":"Triolein","level":4,"score":0.5246999859809875},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.42489999532699585},{"id":"https://openalex.org/C2781044401","wikidata":"https://www.wikidata.org/wiki/Q418897","display_name":"Elastase","level":3,"score":0.40220001339912415},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.38989999890327454},{"id":"https://openalex.org/C2779678110","wikidata":"https://www.wikidata.org/wiki/Q764","display_name":"Fungus","level":2,"score":0.3628000020980835},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.3562000095844269},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3467999994754791},{"id":"https://openalex.org/C2779697368","wikidata":"https://www.wikidata.org/wiki/Q380967","display_name":"Lipase","level":3,"score":0.34119999408721924},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.33329999446868896},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3131999969482422},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.30649998784065247},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2946000099182129},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.2800000011920929},{"id":"https://openalex.org/C198684833","wikidata":"https://www.wikidata.org/wiki/Q2823748","display_name":"Specific activity","level":3,"score":0.27570000290870667},{"id":"https://openalex.org/C2994592520","wikidata":"https://www.wikidata.org/wiki/Q5090496","display_name":"Substrate specificity","level":3,"score":0.27559998631477356},{"id":"https://openalex.org/C2778342517","wikidata":"https://www.wikidata.org/wiki/Q5509914","display_name":"Fusarium solani","level":2,"score":0.26350000500679016},{"id":"https://openalex.org/C2778394675","wikidata":"https://www.wikidata.org/wiki/Q648056","display_name":"Fungi imperfecti","level":2,"score":0.2540000081062317}],"mesh":[],"locations_count":1,"locations":[{"id":"pmh:oai:repository.helmholtz-hzi.de:10033/623722","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10033/623722","pdf_url":"https://repository.helmholtz-hzi.de/bitstream/10033/623722/1/Lauwereys243.pdf","source":{"id":"https://openalex.org/S4377196474","display_name":"HZI (Helmholtz Centre for Infection Research)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210124929","host_organization_name":"Helmholtz Centre for Infection Research","host_organization_lineage":["https://openalex.org/I4210124929"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"conference paper"}],"best_oa_location":{"id":"pmh:oai:repository.helmholtz-hzi.de:10033/623722","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10033/623722","pdf_url":"https://repository.helmholtz-hzi.de/bitstream/10033/623722/1/Lauwereys243.pdf","source":{"id":"https://openalex.org/S4377196474","display_name":"HZI (Helmholtz Centre for Infection Research)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210124929","host_organization_name":"Helmholtz Centre for Infection Research","host_organization_lineage":["https://openalex.org/I4210124929"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"conference paper"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7036615708.pdf","grobid_xml":"https://content.openalex.org/works/W7036615708.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1977165802","https://openalex.org/W1980606004","https://openalex.org/W2135506373","https://openalex.org/W7018121448","https://openalex.org/W1984408980","https://openalex.org/W2379812099","https://openalex.org/W2007923318","https://openalex.org/W4206847236","https://openalex.org/W2024650559","https://openalex.org/W3149157756"],"abstract_inverted_index":{"A":[0],"cutinase":[1],"from":[2,75],"the":[3,21,24,32,47,65,69,84,87,97],"fungus":[4],"Fusarium":[5],"solani":[6],"pisi":[7],"has":[8],"been":[9],"overproduced":[10,48],"in":[11,39,78],"E.":[12],"coli":[13],"by":[14],"placing":[15],"a":[16],"phoA-signal/cutinase":[17],"hybrid":[18],"gene":[19],"under":[20],"control":[22],"of":[23,68,86],"tac":[25],"promoter.":[26],"Due":[27],"to":[28],"its":[29],"periplasmic":[30],"location":[31],"recombinant":[33,98],"enzyme":[34,51,99],"can":[35],"be":[36],"easily":[37],"purified":[38],"large":[40],"quantities.":[41],"Assays":[42],"using":[43],"p-nitrophenylbutyrate":[44],"suggest":[45],"that":[46,79,96],"and":[49,61],"authentic":[50],"are":[52],"catalytically":[53],"equivalent.":[54],"The":[55,71],"specific":[56],"activities":[57],"on":[58],"tributyrin":[59,88],"(4000u/mg)":[60],"triolein":[62],"(800u/mg)":[63],"demonstrate":[64],"lipolytic":[66],"nature":[67],"enzyme.":[70],"cutinase,":[72],"however,":[73],"differs":[74],"classical":[76],"lipases":[77],"no":[80],"measurable":[81],"activation":[82],"around":[83],"CMC":[85],"substrate":[89],"is":[90,100],"observed.":[91],"We":[92],"also":[93],"provide":[94],"evidence":[95],"quite":[101],"thermostable.":[102]},"counts_by_year":[{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2025-10-10T00:00:00"}