{"id":"https://openalex.org/W7009764281","doi":null,"title":"Engineering a responsive, heterologous mevalonate pathway in Escherichia coli using microarrays","display_name":"Engineering a responsive, heterologous mevalonate pathway in Escherichia coli using microarrays","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W7009764281"},"language":"en","primary_location":{"id":"pmh:qt9rc6c93f","doi":null,"is_oa":true,"landing_page_url":"http://www.escholarship.org/uc/item/9rc6c93f","pdf_url":"http://www.escholarship.org/uc/item/9rc6c93f","source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Dahl II, Robert Harlan. (2011). Engineering a responsive, heterologous mevalonate pathway in Escherichia coli using microarrays. UC Berkeley: Chemical Engineering. Retrieved from: http://www.escholarship.org/uc/item/9rc6c93f","raw_type":"dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://www.escholarship.org/uc/item/9rc6c93f","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Dahl II, Robert Harlan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Dahl II, Robert Harlan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.8788999915122986,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.8788999915122986,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.11869999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mevalonate-pathway","display_name":"mevalonate pathway","score":0.9829999804496765},{"id":"https://openalex.org/keywords/isoprenoid-biosynthesis","display_name":"isoprenoid biosynthesis","score":0.8899999856948853},{"id":"https://openalex.org/keywords/farnesyl-pyrophosphate","display_name":"farnesyl pyrophosphate","score":0.8930000066757202},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8090000152587891},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.8619999885559082},{"id":"https://openalex.org/keywords/two-component-systems","display_name":"two-component systems","score":0.5870000123977661},{"id":"https://openalex.org/keywords/magnesium-regulation","display_name":"magnesium regulation","score":0.421999990940094},{"id":"https://openalex.org/keywords/fluorescent-sensors","display_name":"fluorescent sensors","score":0.7440000176429749},{"id":"https://openalex.org/keywords/inducible-promoters","display_name":"inducible promoters","score":0.6819999814033508},{"id":"https://openalex.org/keywords/metabolic-flux-control","display_name":"metabolic flux control","score":0.4050000011920929},{"id":"https://openalex.org/keywords/terpene-synthase","display_name":"terpene synthase","score":0.7260000109672546}],"concepts":[{"id":"https://openalex.org/C62361671","wikidata":"https://www.wikidata.org/wiki/Q1754612","display_name":"Regulon","level":4,"score":0.8639000058174133},{"id":"https://openalex.org/C2779982574","wikidata":"https://www.wikidata.org/wiki/Q14913010","display_name":"Mevalonate pathway","level":4,"score":0.7799000144004822},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7135999798774719},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.6808000206947327},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6410999894142151},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5292999744415283},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4977000057697296},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.4693000018596649},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.4632999897003174},{"id":"https://openalex.org/C2775933503","wikidata":"https://www.wikidata.org/wiki/Q25113781","display_name":"Farnesyl diphosphate synthase","level":4,"score":0.4388999938964844},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.43130001425743103},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.4203000068664551},{"id":"https://openalex.org/C8415881","wikidata":"https://www.wikidata.org/wiki/Q6839217","display_name":"Microarray analysis techniques","level":4,"score":0.4189999997615814},{"id":"https://openalex.org/C206103511","wikidata":"https://www.wikidata.org/wiki/Q426694","display_name":"Terpenoid","level":2,"score":0.40860000252723694},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.3490000069141388},{"id":"https://openalex.org/C192989942","wikidata":"https://www.wikidata.org/wiki/Q68685","display_name":"Metabolic pathway","level":3,"score":0.3479999899864197},{"id":"https://openalex.org/C95371953","wikidata":"https://www.wikidata.org/wiki/Q591745","display_name":"DNA microarray","level":4,"score":0.3287000060081482},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3199999928474426},{"id":"https://openalex.org/C186836561","wikidata":"https://www.wikidata.org/wiki/Q1931516","display_name":"Microarray","level":4,"score":0.29280000925064087},{"id":"https://openalex.org/C2776030549","wikidata":"https://www.wikidata.org/wiki/Q7829395","display_name":"Nutrient sensing","level":3,"score":0.289900004863739},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.2840000092983246},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2815000116825104},{"id":"https://openalex.org/C9927688","wikidata":"https://www.wikidata.org/wiki/Q4915012","display_name":"Biological pathway","level":4,"score":0.27489998936653137},{"id":"https://openalex.org/C158448853","wikidata":"https://www.wikidata.org/wiki/Q425218","display_name":"Repressor","level":4,"score":0.27459999918937683},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.2596000134944916},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.2590999901294708},{"id":"https://openalex.org/C183978625","wikidata":"https://www.wikidata.org/wiki/Q903596","display_name":"Electrophoretic mobility shift assay","level":4,"score":0.25459998846054077},{"id":"https://openalex.org/C27153228","wikidata":"https://www.wikidata.org/wiki/Q3787274","display_name":"Transcriptional regulation","level":4,"score":0.25209999084472656}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:qt9rc6c93f","doi":null,"is_oa":true,"landing_page_url":"http://www.escholarship.org/uc/item/9rc6c93f","pdf_url":"http://www.escholarship.org/uc/item/9rc6c93f","source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Dahl II, Robert Harlan. (2011). Engineering a responsive, heterologous mevalonate pathway in Escherichia coli using microarrays. UC Berkeley: Chemical Engineering. Retrieved from: http://www.escholarship.org/uc/item/9rc6c93f","raw_type":"dissertation"},{"id":"pmh:oai:escholarship.org/ark:/13030/qt9rc6c93f","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/9rc6c93f","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"}],"best_oa_location":{"id":"pmh:qt9rc6c93f","doi":null,"is_oa":true,"landing_page_url":"http://www.escholarship.org/uc/item/9rc6c93f","pdf_url":"http://www.escholarship.org/uc/item/9rc6c93f","source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Dahl II, Robert Harlan. (2011). Engineering a responsive, heterologous mevalonate pathway in Escherichia coli using microarrays. UC Berkeley: Chemical Engineering. Retrieved from: http://www.escholarship.org/uc/item/9rc6c93f","raw_type":"dissertation"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7009764281.pdf","grobid_xml":"https://content.openalex.org/works/W7009764281.grobid-xml"},"referenced_works_count":130,"referenced_works":["https://openalex.org/W1095145","https://openalex.org/W4366438","https://openalex.org/W97387340","https://openalex.org/W644442692","https://openalex.org/W651040357","https://openalex.org/W1506160581","https://openalex.org/W1537110470","https://openalex.org/W1547106545","https://openalex.org/W1565030705","https://openalex.org/W1579030151","https://openalex.org/W1584250038","https://openalex.org/W1606097256","https://openalex.org/W1645305843","https://openalex.org/W1917978909","https://openalex.org/W1965332656","https://openalex.org/W1966786968","https://openalex.org/W1967946076","https://openalex.org/W1970156673","https://openalex.org/W1974809350","https://openalex.org/W1975830907","https://openalex.org/W1976611911","https://openalex.org/W1977624796","https://openalex.org/W1978396622","https://openalex.org/W1979159575","https://openalex.org/W1980658348","https://openalex.org/W1981471795","https://openalex.org/W1981509058","https://openalex.org/W1982309495","https://openalex.org/W1982658095","https://openalex.org/W1982836457","https://openalex.org/W1988067659","https://openalex.org/W1989473753","https://openalex.org/W1994106324","https://openalex.org/W1994782227","https://openalex.org/W1995830102","https://openalex.org/W1996922732","https://openalex.org/W1997874823","https://openalex.org/W1998908967","https://openalex.org/W1999258193","https://openalex.org/W1999404678","https://openalex.org/W1999642437","https://openalex.org/W2000004958","https://openalex.org/W2007493979","https://openalex.org/W2014177423","https://openalex.org/W2014968469","https://openalex.org/W2020541351","https://openalex.org/W2021110283","https://openalex.org/W2024675792","https://openalex.org/W2026829407","https://openalex.org/W2027955923","https://openalex.org/W2028343312","https://openalex.org/W2029392258","https://openalex.org/W2030794862","https://openalex.org/W2033134248","https://openalex.org/W2033646322","https://openalex.org/W2033766977","https://openalex.org/W2033883546","https://openalex.org/W2036502774","https://openalex.org/W2038186289","https://openalex.org/W2038613897","https://openalex.org/W2039144162","https://openalex.org/W2042262705","https://openalex.org/W2044525257","https://openalex.org/W2047360542","https://openalex.org/W2049264625","https://openalex.org/W2049829444","https://openalex.org/W2050383608","https://openalex.org/W2052199039","https://openalex.org/W2053818267","https://openalex.org/W2055304344","https://openalex.org/W2062221023","https://openalex.org/W2064843471","https://openalex.org/W2074244307","https://openalex.org/W2074955506","https://openalex.org/W2075958100","https://openalex.org/W2083870761","https://openalex.org/W2086652584","https://openalex.org/W2086760563","https://openalex.org/W2088475678","https://openalex.org/W2091139369","https://openalex.org/W2092533253","https://openalex.org/W2092808125","https://openalex.org/W2098390695","https://openalex.org/W2098667036","https://openalex.org/W2100636437","https://openalex.org/W2106246537","https://openalex.org/W2106653478","https://openalex.org/W2111870329","https://openalex.org/W2113310577","https://openalex.org/W2113381189","https://openalex.org/W2113746284","https://openalex.org/W2115286109","https://openalex.org/W2115419697","https://openalex.org/W2116137883","https://openalex.org/W2117212863","https://openalex.org/W2118764014","https://openalex.org/W2121806138","https://openalex.org/W2122170499","https://openalex.org/W2124078475","https://openalex.org/W2124977728","https://openalex.org/W2125108766","https://openalex.org/W2128682770","https://openalex.org/W2128737924","https://openalex.org/W2131350482","https://openalex.org/W2132828256","https://openalex.org/W2134701031","https://openalex.org/W2134783465","https://openalex.org/W2136536485","https://openalex.org/W2138059406","https://openalex.org/W2138284860","https://openalex.org/W2143197862","https://openalex.org/W2143325213","https://openalex.org/W2144782152","https://openalex.org/W2146061786","https://openalex.org/W2148641266","https://openalex.org/W2149202606","https://openalex.org/W2149856760","https://openalex.org/W2150013803","https://openalex.org/W2158721036","https://openalex.org/W2158880948","https://openalex.org/W2162095292","https://openalex.org/W2163732235","https://openalex.org/W2164306370","https://openalex.org/W2167090382","https://openalex.org/W2172162088","https://openalex.org/W2189293602","https://openalex.org/W4240480198","https://openalex.org/W4248500926","https://openalex.org/W4249990043","https://openalex.org/W4294107304"],"related_works":["https://openalex.org/W336228064","https://openalex.org/W1978396622","https://openalex.org/W2984650430","https://openalex.org/W2098390695","https://openalex.org/W2092122738","https://openalex.org/W4403820585","https://openalex.org/W6996799039","https://openalex.org/W2067568255","https://openalex.org/W3118761958","https://openalex.org/W2898924116"],"abstract_inverted_index":{"Isoprenoids":[0],"are":[1],"a":[2,97,144,303],"highly":[3],"diverse":[4],"class":[5],"of":[6,39,57,109,118,129,135,170,202,243,282,329,361],"natural":[7,34],"compounds":[8],"that":[9,175,199,229,301],"have":[10,86,296,313],"received":[11],"wide":[12],"spread":[13],"commercial":[14],"use":[15,328],"as":[16,143,226],"flavors,":[17],"fragrances,":[18],"and":[19,36,77,114,182,194,224,247,268,312,346,367,418],"pharmaceuticals.":[20],"However,":[21,322,388],"their":[22,29,33,81,260],"supply":[23],"is":[24,153,333],"often":[25],"limited":[26],"due":[27,125],"to":[28,53,89,126,147,160,162,196,251,264,274,285,291,308,316,343,402],"low":[30],"abundance":[31],"in":[32,96,122,165,185,214,249,289,306],"sources":[35],"difficult":[37],"routes":[38],"chemical":[40],"synthesis.":[41],"To":[42],"alleviate":[43],"this":[44],"limitation,":[45],"the":[46,58,61,66,87,106,115,119,127,130,136,140,148,166,171,176,180,186,191,197,200,203,209,215,276,280,287,292,317,320,327,337,344,348,355,362,368,374,381,385,390,396,403,408],"Keasling":[47],"Lab":[48],"has":[49],"engineered":[50,74,101],"Escherichia":[51],"coli":[52],"produce":[54],"large":[55],"quantities":[56],"ultimate":[59],"precursors,":[60],"isoprenyl":[62,131,363],"diphosphates,":[63],"by":[64,373,395],"introducing":[65],"mevalonate":[67],"pathway":[68,103,113,120,288,345,369,392,410],"from":[69,80,93],"Saccharomyces":[70],"cerevisiae.":[71],"Coupled":[72],"with":[73,219],"terpene":[75],"synthases":[76],"tailoring":[78],"enzymes":[79],"native":[82,107],"sources,":[83],"we":[84,295],"now":[85],"ability":[88],"make":[90],"these":[91,244,269,330],"molecules":[92,207],"simple":[94],"sugars":[95],"controlled":[98],"environment.":[99,167],"\\tThe":[100],"heterologous":[102,277],"however":[104],"bypassed":[105],"regulation":[108,342],"E.":[110,154],"coli's":[111,155],"isoprenoid":[112],"unregulated":[116],"expression":[117],"resulted":[121],"growth":[123,172],"inhibition":[124],"accumulation":[128,201,267],"diphosphates.":[132],"Microarray":[133],"analysis":[134,169],"inhibited":[137],"cells":[138],"identified":[139],"PhoPQ":[141,151],"regulon":[142,152],"concerted":[145],"response":[146,250,263,290,307],"toxicity.":[149],"The":[150,255],"regulatory":[156],"two":[157],"component":[158],"system":[159],"respond":[161],"magnesium":[163,181,225],"concentrations":[164,184],"Further":[168],"conditions":[173],"revealed":[174],"toxicity":[177],"occurred":[178],"when":[179,389],"glucose":[183,416],"media":[187],"were":[188,239,257,271,358],"low,":[189],"validating":[190],"microarray":[192],"results":[193],"leading":[195],"hypothesis":[198],"negatively":[204],"charged":[205],"diphosphate":[206,231,253],"disrupted":[208],"divalent":[210],"cation's":[211],"normal":[212],"role":[213],"cell.":[216],"Enzyme":[217],"assays":[218],"luciferase,":[220],"which":[221],"uses":[222],"ATP":[223],"cofactors,":[227],"confirmed":[228],"farnesyl":[230,252,265],"was":[232,370,378,393],"inhibitory":[233],"at":[234,323],"an":[235,309,351],"enzyme":[236,366],"level.\\tFluorescent":[237],"sensors":[238,256],"designed":[240],"using":[241],"promoters":[242,270,300,339,357],"genes":[245],"upregulated":[246],"downregulated":[248],"accumulation.":[254],"tested":[258],"for":[259,350],"dose":[261],"dependent":[262],"diphoshpate":[266],"then":[272],"used":[273,297],"regulate":[275,286],"pathway.":[278,321,405],"In":[279,406],"absence":[281],"other":[283],"methods":[284],"intermediates'":[293],"accumulation,":[294,413],"standard":[298],"inducible":[299,375,382,404],"give":[302],"static":[304],"output":[305],"exogenous":[310,331,352],"inducer":[311],"no":[314],"connection":[315],"flux":[318],"through":[319],"industrial":[324],"scale":[325],"production":[326,377,399],"inducers":[332],"not":[334],"feasible.":[335],"Using":[336],"responsive":[338,356,397,409],"return":[340],"dynamic":[341],"obviates":[347],"need":[349],"inducer.":[353],"When":[354],"cloned":[359],"5\u2019":[360],"diphsophates'":[364],"downstream":[365,386],"still":[371],"regulated":[372,394],"promoters,":[376,398],"less":[379],"than":[380],"promoter":[383],"regulating":[384],"enzyme.":[387],"entire":[391],"doubled":[400],"relative":[401],"addition,":[407],"reduced":[411],"acetate":[412],"allowed":[414],"faster":[415],"consumption,":[417],"increased":[419],"cell":[420],"growth.":[421]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2025-10-10T00:00:00"}