{"id":"https://openalex.org/W6996421522","doi":null,"title":"Ruolo delle glicoproteine di involucro virale nella maturazione dei vettori lentivirali pseudotipizzati","display_name":"Ruolo delle glicoproteine di involucro virale nella maturazione dei vettori lentivirali pseudotipizzati","publication_year":2024,"publication_date":"2024-04-03","ids":{"openalex":"https://openalex.org/W6996421522"},"language":"it","primary_location":{"id":"pmh:oai:etd.adm.unipi.it:etd-03172024-193624","doi":null,"is_oa":true,"landing_page_url":"https://etd.adm.unipi.it/theses/available/etd-03172024-193624/","pdf_url":"https://etd.adm.unipi.it/theses/available/etd-03172024-193624/unrestricted/MatteoMatteucci.pdf","source":{"id":"https://openalex.org/S4306401800","display_name":"Electronic Theses and Dissertations Repository (University of Pisa)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I108290504","host_organization_name":"University of Pisa","host_organization_lineage":["https://openalex.org/I108290504"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"https://etd.adm.unipi.it/theses/available/etd-03172024-193624/","raw_type":"text"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://etd.adm.unipi.it/theses/available/etd-03172024-193624/unrestricted/MatteoMatteucci.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"MATTEUCCI, MATTEO","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"MATTEUCCI, MATTEO","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.002199999988079071,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lentiviral-vectors","display_name":"lentiviral vectors","score":0.9739999771118164},{"id":"https://openalex.org/keywords/viral-envelope-glycoprotein","display_name":"viral envelope glycoprotein","score":0.8600000143051147},{"id":"https://openalex.org/keywords/vsv-g","display_name":"vesicular stomatitis virus glycoprotein","score":0.8579999804496765},{"id":"https://openalex.org/keywords/hiv-1-envelope-glycoprotein","display_name":"HIV-1 envelope glycoprotein","score":0.765999972820282},{"id":"https://openalex.org/keywords/capsid-maturation","display_name":"capsid maturation","score":0.7900000214576721},{"id":"https://openalex.org/keywords/protease-cleavage","display_name":"protease cleavage","score":0.5709999799728394},{"id":"https://openalex.org/keywords/transduction-efficiency","display_name":"transduction efficiency","score":0.5509999990463257},{"id":"https://openalex.org/keywords/hiv-1-reverse-transcriptase","display_name":"HIV-1 reverse transcriptase","score":0.44999998807907104},{"id":"https://openalex.org/keywords/hiv-1-integrase","display_name":"HIV-1 integrase","score":0.6420000195503235},{"id":"https://openalex.org/keywords/hiv-1-protease","display_name":"HIV-1 protease","score":0.6660000085830688},{"id":"https://openalex.org/keywords/hiv-1-vpr","display_name":"HIV-1 Vpr","score":0.47699999809265137},{"id":"https://openalex.org/keywords/hiv-1-vif","display_name":"HIV-1 Vif","score":0.640999972820282},{"id":"https://openalex.org/keywords/hiv-1-vpu","display_name":"HIV-1 Vpu","score":0.8119999766349792}],"concepts":[{"id":"https://openalex.org/C15708023","wikidata":"https://www.wikidata.org/wiki/Q80083","display_name":"Humanities","level":1,"score":0.492000013589859},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.43149998784065247},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40220001339912415},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.2468000054359436},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.24549999833106995},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.17890000343322754},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1720000058412552},{"id":"https://openalex.org/C2908941092","wikidata":"https://www.wikidata.org/wiki/Q1928978","display_name":"Virus diseases","level":3,"score":0.15479999780654907},{"id":"https://openalex.org/C29456083","wikidata":"https://www.wikidata.org/wiki/Q1221899","display_name":"Gynecology","level":1,"score":0.14910000562667847},{"id":"https://openalex.org/C2911197035","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Immune serums","level":4,"score":0.14890000224113464}],"mesh":[],"locations_count":1,"locations":[{"id":"pmh:oai:etd.adm.unipi.it:etd-03172024-193624","doi":null,"is_oa":true,"landing_page_url":"https://etd.adm.unipi.it/theses/available/etd-03172024-193624/","pdf_url":"https://etd.adm.unipi.it/theses/available/etd-03172024-193624/unrestricted/MatteoMatteucci.pdf","source":{"id":"https://openalex.org/S4306401800","display_name":"Electronic Theses and Dissertations Repository (University of Pisa)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I108290504","host_organization_name":"University of Pisa","host_organization_lineage":["https://openalex.org/I108290504"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"https://etd.adm.unipi.it/theses/available/etd-03172024-193624/","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:etd.adm.unipi.it:etd-03172024-193624","doi":null,"is_oa":true,"landing_page_url":"https://etd.adm.unipi.it/theses/available/etd-03172024-193624/","pdf_url":"https://etd.adm.unipi.it/theses/available/etd-03172024-193624/unrestricted/MatteoMatteucci.pdf","source":{"id":"https://openalex.org/S4306401800","display_name":"Electronic Theses and Dissertations Repository (University of Pisa)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I108290504","host_organization_name":"University of Pisa","host_organization_lineage":["https://openalex.org/I108290504"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"https://etd.adm.unipi.it/theses/available/etd-03172024-193624/","raw_type":"text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W6996421522.pdf","grobid_xml":"https://content.openalex.org/works/W6996421522.grobid-xml"},"referenced_works_count":124,"referenced_works":["https://openalex.org/W1511364129","https://openalex.org/W1517043843","https://openalex.org/W1544126343","https://openalex.org/W1544464869","https://openalex.org/W1548281677","https://openalex.org/W1567879434","https://openalex.org/W1587831715","https://openalex.org/W1632486555","https://openalex.org/W1823429634","https://openalex.org/W1837543172","https://openalex.org/W1964212318","https://openalex.org/W1970153632","https://openalex.org/W1971421092","https://openalex.org/W1975188073","https://openalex.org/W1975535299","https://openalex.org/W1977218442","https://openalex.org/W1980268459","https://openalex.org/W1981201701","https://openalex.org/W1981605051","https://openalex.org/W1984944166","https://openalex.org/W1990996124","https://openalex.org/W1994189876","https://openalex.org/W1994354502","https://openalex.org/W1997505475","https://openalex.org/W1998271864","https://openalex.org/W1999460219","https://openalex.org/W2002671363","https://openalex.org/W2004669329","https://openalex.org/W2006519535","https://openalex.org/W2009761723","https://openalex.org/W2010655741","https://openalex.org/W2011802533","https://openalex.org/W2016719543","https://openalex.org/W2017924235","https://openalex.org/W2021235760","https://openalex.org/W2023290813","https://openalex.org/W2024130707","https://openalex.org/W2024624336","https://openalex.org/W2026855099","https://openalex.org/W2032428846","https://openalex.org/W2047924237","https://openalex.org/W2052389336","https://openalex.org/W2052678796","https://openalex.org/W2058768365","https://openalex.org/W2062287169","https://openalex.org/W2063794379","https://openalex.org/W2066027429","https://openalex.org/W2069017877","https://openalex.org/W2070131396","https://openalex.org/W2074047418","https://openalex.org/W2075932312","https://openalex.org/W2081499342","https://openalex.org/W2082607586","https://openalex.org/W2086253922","https://openalex.org/W2093456378","https://openalex.org/W2094692623","https://openalex.org/W2098447112","https://openalex.org/W2101702737","https://openalex.org/W2102549543","https://openalex.org/W2102838769","https://openalex.org/W2103368202","https://openalex.org/W2105565651","https://openalex.org/W2108772360","https://openalex.org/W2117867057","https://openalex.org/W2136030671","https://openalex.org/W2136744565","https://openalex.org/W2144975624","https://openalex.org/W2147614247","https://openalex.org/W2148259768","https://openalex.org/W2154853638","https://openalex.org/W2166265186","https://openalex.org/W2166420278","https://openalex.org/W2166822904","https://openalex.org/W2171743890","https://openalex.org/W2320452088","https://openalex.org/W2336000508","https://openalex.org/W2425381195","https://openalex.org/W2469904508","https://openalex.org/W2514377229","https://openalex.org/W2524642230","https://openalex.org/W2550560662","https://openalex.org/W2614294198","https://openalex.org/W2620136843","https://openalex.org/W2791408070","https://openalex.org/W2793411781","https://openalex.org/W2793508531","https://openalex.org/W2793675608","https://openalex.org/W2799371530","https://openalex.org/W2807667147","https://openalex.org/W2888914380","https://openalex.org/W2900776747","https://openalex.org/W2915813426","https://openalex.org/W2940746649","https://openalex.org/W2977477797","https://openalex.org/W2987456730","https://openalex.org/W3003217347","https://openalex.org/W3004621155","https://openalex.org/W3013093478","https://openalex.org/W3014660159","https://openalex.org/W3024961866","https://openalex.org/W3046463683","https://openalex.org/W3047015956","https://openalex.org/W3049198861","https://openalex.org/W3091399490","https://openalex.org/W3100064532","https://openalex.org/W3121652846","https://openalex.org/W3126674454","https://openalex.org/W3155408009","https://openalex.org/W3157219655","https://openalex.org/W3202862945","https://openalex.org/W3206816930","https://openalex.org/W4200007197","https://openalex.org/W4205791358","https://openalex.org/W4213190829","https://openalex.org/W4295280477","https://openalex.org/W4297236849","https://openalex.org/W4298088405","https://openalex.org/W4303699509","https://openalex.org/W4304189840","https://openalex.org/W4304208539","https://openalex.org/W4306385440","https://openalex.org/W4318981565","https://openalex.org/W4323851568","https://openalex.org/W4382678115"],"related_works":["https://openalex.org/W3093022484","https://openalex.org/W2112936117","https://openalex.org/W7095207393","https://openalex.org/W4241178593","https://openalex.org/W2093721962","https://openalex.org/W2082139926","https://openalex.org/W4237944944","https://openalex.org/W2913306627","https://openalex.org/W2030866906","https://openalex.org/W7120869815"],"abstract_inverted_index":{"I":[0,19],"vettori":[1],"lentivirali":[2],"(LV)":[3],"sono":[4,566],"uno":[5],"strumento":[6],"fondamentale":[7],"utilizzato":[8,417],"per":[9,38,161,207,251,256,418,442,575,605],"la":[10,39,54,109,113,117,136,157,162,167,181,252,257,272,278,284,299,316,325,359,371,377,395,419,424,443,472,475,495,501,515,576],"veicolazione":[11,258],"di":[12,30,42,86,126,133,147,152,183,188,198,202,233,236,242,254,259,263,280,301,304,313,318,322,328,330,334,336,347,363,381,391,397,402,427,430,450,461,531,544,563,573,583,591,602,608,614],"materiale":[13,260],"genetico":[14,261],"all\u2019interno":[15,262],"delle":[16,383,482,517,581],"cellule":[17,47],"eucariotiche.":[18],"LV":[20,127],"possono":[21],"essere":[22,486],"derivati":[23],"dalla":[24,223],"modificazione":[25],"del":[26,49,170,200,287,445,478,498],"virus":[27,171,220,288],"dell\u2019immunodeficienza":[28],"umana":[29],"tipo":[31],"1":[32],"(HIV-1),":[33],"un":[34,120,145,185,208,246,571],"patogeno":[35],"tristemente":[36],"conosciuto":[37],"sua":[40,595],"abilit\u00e0":[41],"infettare":[43],"e":[44,58,77,92,112,324,338,355,379,474,503],"distruggere":[45],"le":[46],"CD4+":[48],"sistema":[50],"immunitario,":[51],"compromettendo":[52],"progressivamente":[53],"risposta":[55],"immunitaria":[56],"dell'ospite":[57],"portando":[59],"alla":[60,309,408],"comparsa":[61],"dell'AIDS":[62],"(Acquired":[63],"Immune":[64],"Deficiency":[65],"Syndrome),":[66],"una":[67,130,216],"condizione":[68],"debilitante":[69],"che":[70,122,204,218,471,610],"rende":[71],"l'organismo":[72],"suscettibile":[73],"a":[74,178,244,274,295,361,413,468,539,594],"infezioni":[75],"opportuniste":[76],"neoplasie.":[78],"Per":[79],"rendere":[80],"sicuri":[81],"i":[82,366,568],"LV,":[83],"il":[84,196,219,388,524,541,612],"genoma":[85],"HIV-1":[87,103,203],"\u00e8":[88,119,142,166,221,226,238,342,404,520,535],"stato":[89,239,536],"profondamente":[90],"modificato":[91],"frammentato":[93],"nelle":[94],"sue":[95],"diverse":[96,191,264,319,483,589],"componenti":[97],"fondamentali.":[98],"Le":[99],"modifiche":[100],"rispetto":[101],"ad":[102],"riguardano":[104],"l\u2019inattivazione":[105],"della":[106,150,172,213,437,546],"capacit\u00e0":[107,182],"replicativa,":[108],"riduzione":[110],"dell\u2019infettivit\u00e0":[111],"pseudotipizzazione.":[114],"In":[115,270,393,558],"particolare,":[116,271,394],"pseudotipizzazione":[118],"processo":[121,197,613],"consiste":[123],"nella":[124,488],"produzione":[125,163,253,279,310,378,574],"caratterizzati":[128],"da":[129,144,211,422],"diversa":[131,372],"glicoproteina":[132,151,158,168,285],"Envelope":[134],"(Env);":[135],"risultante":[137],"particella":[138],"virale":[139,215],"pseudotipizzata":[140],"(PLV)":[141],"caratterizzata":[143],"tropismo":[146],"trasduzione":[148,329,339,382],"caratteristico":[149],"Env":[153,373,484,510],"utilizzata.":[154],"Ad":[155],"esempio,":[156],"pi\u00f9":[159],"utilizzata":[160],"dei":[164,306,311,340,410,439,490,506,533,553,585],"PLV":[165,180,255,281,341,398,411,441,534,554,569,586],"G":[169],"stomatite":[173],"vescicolare":[174],"(VSV-G).":[175],"Essa":[176],"conferisce":[177],"questi":[179,364],"trasdurre":[184],"ampio":[186],"spettro":[187],"tipologie":[189],"cellulari":[190,266,321],"con":[192,283,291,400,511,588],"elevata":[193],"efficienza.":[194],"Invece,":[195],"maturazione":[199,396,409,438,462,489,532,584,615],"capside":[201],"normalmente":[205],"avviene":[206],"taglio":[209],"proteolitico":[210],"parte":[212,426],"proteasi":[214],"volta":[217],"gemmato":[222],"cellula":[224],"ospite,":[225],"conservato":[227],"anche":[228,387],"nei":[229],"PLV.":[230],"Lo":[231],"scopo":[232],"questo":[234,428,451,561],"elaborato":[235,429,562],"tesi":[237,431,564],"inizialmente":[240],"quello":[241],"mettere":[243],"punto":[245,275,296,362],"protocollo":[247,416,572],"efficace":[248],"ed":[249,555],"affidabile":[250],"linee":[265,320],"utilizzate":[267],"in":[268,560,578],"laboratorio.":[269],"messa":[273,294,360],"ha":[276,297],"riguardato":[277,298],"tipizzati":[282],"Spike":[286,401,502],"SARS-Cov-2":[289],"o":[290,514],"VSV-G.":[292,504],"La":[293,448],"valutazione":[300,317,326,577],"diversi":[302,331,440],"rapporti":[303],"trasfezione":[305],"plasmidi":[307,508],"necessari":[308],"PLVs":[312],"terza":[314],"generazione,":[315],"packaging":[323],"dell\u2019efficienza":[327],"transgeni.":[332],"L\u2019efficienza":[333],"produzione,":[335],"impacchettamento":[337],"stata":[343,521],"valutata":[344],"tramite":[345,549],"tecniche":[346],"Western":[348],"Blotting":[349],"(WB),":[350],"immunofluorescenza":[351],"confocale":[352],"high":[353],"throughput":[354],"citofluorimetria":[356],"FACS.":[357],"Durante":[358],"protocolli,":[365],"risultati":[367],"hanno":[368],"dimostrato":[369],"come":[370],"influenzi":[374],"non":[375],"solo":[376],"l\u2019efficienza":[380],"particelle":[384],"lentivirali,":[385],"ma":[386],"loro":[389,518],"grado":[390,530,543],"maturazione.":[392],"pseudotipizzati":[399,587],"SARS-CoV-2":[403],"risultata":[405],"sempre":[406],"superiore":[407],"VSV-G":[412],"prescindere":[414],"dal":[415],"produzione.":[420],"Partendo":[421],"questo,":[423],"seconda":[425],"vuole":[432],"far":[433],"luce":[434],"sul":[435],"fenomeno":[436,452],"caratterizzazione":[444],"meccanismo":[446,460],"coinvolto.":[447],"comprensione":[449],"potrebbe":[453,597],"avere":[454,598],"potenziali":[455],"ripercussioni":[456,599],"sulla":[457,600],"conoscenza":[458],"dell\u2019elusivo":[459],"dello":[463,616],"stesso":[464,617],"HIV-1.":[465,618],"Prime":[466],"ipotesi":[467],"riguardo":[469],"suggeriscono":[470],"grandezza":[473],"composizione":[476],"amminoacidica":[477],"frammento":[479],"citoplasmatico":[480],"(CT)":[481],"possa":[485],"coinvolto":[487],"LV.":[491],"Con":[492],"l\u2019interscambio":[493],"e/o":[494],"completa":[496],"delezione":[497,516],"CT":[499,519],"tra":[500],"L\u2019ingegnerizzazione":[505],"due":[507],"codificanti":[509],"lo":[512,606],"scambio":[513],"effettuata":[522],"utilizzando":[523],"kit":[525],"commerciale":[526],"In-Fusion.":[527],"Il":[528],"diverso":[529,542],"valutato":[537],"andando":[538],"vedere":[540],"processazione":[545],"proteina":[547],"p24":[548],"WB,":[550],"previa":[551],"precipitazione":[552],"estrazione":[556],"proteica.":[557],"conclusione,":[559],"si":[565],"utilizzati":[567],"ottimizzandone":[570],"ultima":[579],"analisi":[580],"differenze":[582],"glicoproteine":[590],"Env.":[592],"Questo,":[593],"volta,":[596],"scoperta":[601],"nuovi":[603],"bersagli":[604],"sviluppo":[607],"antivirali":[609],"ostacolino":[611]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2025-10-10T00:00:00"}