{"id":"https://openalex.org/W6991828419","doi":null,"title":"Introduction of a constitutive red fluorescent marker gene into four Variovorax strains using the Tn7 transposon.","display_name":"Introduction of a constitutive red fluorescent marker gene into four Variovorax strains using the Tn7 transposon.","publication_year":2025,"publication_date":"2025-04-26","ids":{"openalex":"https://openalex.org/W6991828419"},"language":"en","primary_location":{"id":"pmh:oai:scholarlycommons.pacific.edu:rcs-1183","doi":null,"is_oa":false,"landing_page_url":"https://scholarlycommons.pacific.edu/rcs/2025/events/104","pdf_url":null,"source":{"id":"https://openalex.org/S4306400314","display_name":"Scholarly Commons (University of the Pacific)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I119942576","host_organization_name":"University of the Pacific","host_organization_lineage":["https://openalex.org/I119942576"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research & Creativity Showcase","raw_type":"text"},"volume":null,"type":"article","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Deviatykh, Dasha","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119942576","display_name":"University of the Pacific","ror":"https://ror.org/05ma4gw77","country_code":"US","type":"education","lineage":["https://openalex.org/I119942576"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Deviatykh, Dasha","raw_affiliation_strings":["University of the Pacific"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of the Pacific","institution_ids":["https://openalex.org/I119942576"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5133931559","display_name":"Ellamae Fortin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119942576","display_name":"University of the Pacific","ror":"https://ror.org/05ma4gw77","country_code":"US","type":"education","lineage":["https://openalex.org/I119942576"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Fortin, Ellamae","raw_affiliation_strings":["University of the Pacific"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of the Pacific","institution_ids":["https://openalex.org/I119942576"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Pidlaoan, Maryann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119942576","display_name":"University of the Pacific","ror":"https://ror.org/05ma4gw77","country_code":"US","type":"education","lineage":["https://openalex.org/I119942576"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Pidlaoan, Maryann","raw_affiliation_strings":["University of the Pacific"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of the Pacific","institution_ids":["https://openalex.org/I119942576"]}]},{"author_position":"middle","author":{"id":null,"display_name":"So, Megan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119942576","display_name":"University of the Pacific","ror":"https://ror.org/05ma4gw77","country_code":"US","type":"education","lineage":["https://openalex.org/I119942576"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"So, Megan","raw_affiliation_strings":["University of the Pacific"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of the Pacific","institution_ids":["https://openalex.org/I119942576"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005048756","display_name":"Paul M. Orwin","orcid":"https://orcid.org/0000-0002-5435-9655","observed_orcids":["https://orcid.org/0000-0002-5435-9655"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Orwin, Paul","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.40083062,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9833999872207642,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9833999872207642,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.008100000210106373,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/variovorax-paradoxus","display_name":"Variovorax paradoxus","score":0.9559999704360962},{"id":"https://openalex.org/keywords/tn7-transposon","display_name":"Tn7 transposon","score":0.9599999785423279},{"id":"https://openalex.org/keywords/dsred","display_name":"DsRed","score":0.6299999952316284},{"id":"https://openalex.org/keywords/gentamicin-resistance","display_name":"gentamicin resistance","score":0.9319999814033508},{"id":"https://openalex.org/keywords/horizontal-gene-transfer","display_name":"horizontal gene transfer","score":0.6769999861717224},{"id":"https://openalex.org/keywords/rhizosphere-bacteria","display_name":"rhizosphere bacteria","score":0.7919999957084656},{"id":"https://openalex.org/keywords/arabidopsis","display_name":"Arabidopsis","score":0.7689999938011169},{"id":"https://openalex.org/keywords/bioremediation","display_name":"bioremediation","score":0.9210000038146973},{"id":"https://openalex.org/keywords/bacterial-competence","display_name":"bacterial competence","score":0.531000018119812},{"id":"https://openalex.org/keywords/conjugation","display_name":"conjugation","score":0.9330000281333923},{"id":"https://openalex.org/keywords/electroporation","display_name":"electroporation","score":0.7409999966621399}],"concepts":[{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.6668000221252441},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5892000198364258},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5455999970436096},{"id":"https://openalex.org/C2780920689","wikidata":"https://www.wikidata.org/wiki/Q27094615","display_name":"Kanamycin","level":3,"score":0.5315999984741211},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4966000020503998},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.486299991607666},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.45210000872612},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.4359000027179718},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.42879998683929443},{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.4058000147342682},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.37929999828338623},{"id":"https://openalex.org/C92938381","wikidata":"https://www.wikidata.org/wiki/Q83185","display_name":"Horizontal gene transfer","level":4,"score":0.36820000410079956},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3610000014305115},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.3433000147342682},{"id":"https://openalex.org/C2775832221","wikidata":"https://www.wikidata.org/wiki/Q422482","display_name":"Gentamicin","level":3,"score":0.31459999084472656},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3025999963283539},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.30079999566078186},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.2944999933242798},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.2793000042438507},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.27799999713897705},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.272599995136261},{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.26010000705718994},{"id":"https://openalex.org/C180223184","wikidata":"https://www.wikidata.org/wiki/Q4839986","display_name":"Bacterial genetics","level":4,"score":0.2565999925136566},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.2379000037908554},{"id":"https://openalex.org/C4918238","wikidata":"https://www.wikidata.org/wiki/Q121438","display_name":"Transposable element","level":4,"score":0.23010000586509705},{"id":"https://openalex.org/C2781212864","wikidata":"https://www.wikidata.org/wiki/Q15551438","display_name":"Panax notoginseng","level":3,"score":0.2273000031709671},{"id":"https://openalex.org/C51456166","wikidata":"https://www.wikidata.org/wiki/Q182726","display_name":"Genetically modified organism","level":3,"score":0.21610000729560852},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.2160000056028366},{"id":"https://openalex.org/C2777787772","wikidata":"https://www.wikidata.org/wiki/Q45355603","display_name":"Aminoglycoside","level":3,"score":0.21469999849796295},{"id":"https://openalex.org/C10879258","wikidata":"https://www.wikidata.org/wiki/Q76826611","display_name":"RNase P","level":4,"score":0.21040000021457672},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.20990000665187836},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20960000157356262},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.19869999587535858},{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.19859999418258667},{"id":"https://openalex.org/C79386861","wikidata":"https://www.wikidata.org/wiki/Q476697","display_name":"DNA profiling","level":3,"score":0.19830000400543213},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.19760000705718994},{"id":"https://openalex.org/C2780923616","wikidata":"https://www.wikidata.org/wiki/Q193045","display_name":"Tetracycline","level":3,"score":0.19519999623298645},{"id":"https://openalex.org/C118083812","wikidata":"https://www.wikidata.org/wiki/Q1650834","display_name":"Insertion","level":4,"score":0.19509999454021454},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.18569999933242798},{"id":"https://openalex.org/C2994225774","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Gene mutation","level":4,"score":0.18330000340938568}],"mesh":[],"locations_count":1,"locations":[{"id":"pmh:oai:scholarlycommons.pacific.edu:rcs-1183","doi":null,"is_oa":false,"landing_page_url":"https://scholarlycommons.pacific.edu/rcs/2025/events/104","pdf_url":null,"source":{"id":"https://openalex.org/S4306400314","display_name":"Scholarly Commons (University of the Pacific)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I119942576","host_organization_name":"University of the Pacific","host_organization_lineage":["https://openalex.org/I119942576"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research & Creativity Showcase","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Life on land","id":"https://metadata.un.org/sdg/15","score":0.5485094785690308}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Variovorax":[0,32,64],"paradoxus":[1],"has":[2,33],"garnered":[3],"much":[4],"interest":[5],"in":[6,19,26,91,99,179],"environmental":[7],"microbiology":[8],"for":[9,47,77,159,225],"its":[10,23],"ability":[11],"to":[12,57,66,128,138,197,213,229,236],"degrade":[13],"organic":[14],"compounds":[15],"and":[16,105,125,136,156,169,181,203,240],"key":[17],"role":[18,25],"the":[20,38,68,113,123,130,144,188,199,207,215,219,231],"rhizosphere.":[21,115],"Despite":[22],"important":[24],"microbial":[27,140],"communities,":[28],"genetic":[29],"manipulation":[30],"of":[31,40,52,87,108,134,187],"been":[34],"proven":[35],"difficult,":[36],"but":[37],"development":[39],"genetically":[41],"engineered":[42],"strains":[43,65,96,127,148,166],"is":[44],"significantly":[45],"promising":[46],"bioremediation":[48],"applications.":[49],"The":[50,80,190],"aim":[51],"our":[53,85,147],"research":[54],"group":[55],"was":[56,177,194],"create":[58],"stable":[59],"mini-Tn7":[60],"insertions":[61],"into":[62,146,201],"several":[63],"express":[67],"red":[69],"fluorescent":[70],"protein":[71],"DsRed":[72],"along":[73,151],"with":[74,152],"a":[75,153],"gene":[76,89],"gentamicin":[78,162],"resistance.":[79],"Tn7":[81,200],"insertion":[82,160,165,232],"will":[83,222],"facilitate":[84],"study":[86],"horizontal":[88],"transfer":[90],"these":[92],"specific":[93],"strains.":[94],"Four":[95],"were":[97,110,167],"used":[98,196,206,224],"this":[100],"study,":[101],"MF004,":[102],"MF295,":[103],"MF278,":[104],"MF375,":[106],"all":[107],"which":[109,221],"isolated":[111],"from":[112,218],"Arabidopsis":[114],"We":[116,142,205],"performed":[117],"minimum":[118],"inhibitory":[119],"concentration":[120,133],"experiments":[121,235],"on":[122,161],"MF004":[124],"MF295":[126],"determine":[129,237],"lowest":[131],"antibiotic":[132],"Kanamycin":[135],"Gentamicin":[137],"inhibit":[139],"growth.":[141],"introduced":[143],"mTn7-DsRed":[145],"by":[149,172],"electroporation":[150],"helper":[154],"plasmid,":[155],"then":[157,170],"selected":[158],"plates.":[163],"Potential":[164],"restreaked":[168],"examined":[171],"fluorescence":[173,176],"microscopy.":[174],"Red":[175],"observed":[178],"colonies":[180],"single":[182],"cells,":[183],"indicating":[184],"successful":[185],"integration":[186],"transposon.":[189],"same":[191],"experimental":[192],"approach":[193],"also":[195,243],"introduce":[198],"MF278":[202],"MF375.":[204],"Wizard":[208],"Genomic":[209],"DNA":[210,217,227],"Purification":[211],"Kit":[212],"extract":[214],"genomic":[216,226],"bacteria,":[220],"be":[223],"sequencing":[228],"verify":[230],"site.":[233],"Preliminary":[234],"bacterial":[238],"competence":[239],"conjugation":[241],"are":[242],"reported":[244],"here.":[245]},"counts_by_year":[],"updated_date":"2026-10-13T10:08:12.033832","created_date":"2025-10-10T00:00:00"}
