{"id":"https://openalex.org/W6977031746","doi":"https://doi.org/10.6084/m9.figshare.5284","title":"Reduction of background by the expression of tTS","display_name":"Reduction of background by the expression of tTS","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W6977031746","doi":"https://doi.org/10.6084/m9.figshare.5284","mag":"2425831749"},"language":"en","primary_location":{"id":"doi:10.6084/m9.figshare.5284","doi":"https://doi.org/10.6084/m9.figshare.5284","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.5284","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Image"},"volume":null,"type":"other","indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.6084/m9.figshare.5284","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Bornkamm, Georg W.","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bornkamm, Georg W.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Berens, Christian","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Berens, Christian","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113679073","display_name":"C. Kuklik-Roos","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kuklik-Roos, Conny","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Bechet, Jean-Marie","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bechet, Jean-Marie","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085334771","display_name":"Gerhard Laux","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Laux, Gerhard","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Bachl, J\u00fcrgen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bachl, J\u00fcrgen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Korndoerfer, Martin","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Korndoerfer, Martin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089335251","display_name":"Martin Schlee","orcid":"https://orcid.org/0000-0003-3671-7639","observed_orcids":["https://orcid.org/0000-0003-3671-7639"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Schlee, Martin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"H\u00f6lzel, Michael","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H\u00f6lzel, Michael","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000667170","display_name":"Malamoussi Anastassia","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Malamoussi, Anastassia","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Chapman, Rob D.","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Chapman, Rob D.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086501578","display_name":"Falk Nimmerjahn","orcid":"https://orcid.org/0000-0002-5418-316X","observed_orcids":["https://orcid.org/0000-0002-5418-316X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nimmerjahn, Falk","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Mautner, Josef","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mautner, Josef","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109973920","display_name":"Wolfgang Hillen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hillen, Wolfgang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011061047","display_name":"Hermann Bujard","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bujard, Hermann","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5023004938","display_name":"Jean Feuillard","orcid":"https://orcid.org/0000-0001-6223-2454","observed_orcids":["https://orcid.org/0000-0001-6223-2454"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Feuillard, Jean","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.22179999947547913,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.22179999947547913,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.19589999318122864,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.12399999797344208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tetracycline-transactivator","display_name":"tetracycline transactivator","score":0.7860000133514404},{"id":"https://openalex.org/keywords/doxycycline-inducible-gene-expression","display_name":"doxycycline-inducible expression","score":0.8960000276565552},{"id":"https://openalex.org/keywords/background-suppression","display_name":"background suppression","score":0.38600000739097595},{"id":"https://openalex.org/keywords/inducibility","display_name":"inducibility","score":0.7590000033378601},{"id":"https://openalex.org/keywords/bjab-cells","display_name":"BJAB cells","score":0.7770000100135803},{"id":"https://openalex.org/keywords/luciferase-reporter","display_name":"luciferase reporter","score":0.7680000066757202},{"id":"https://openalex.org/keywords/egfp-expression","display_name":"EGFP expression","score":0.7870000004768372}],"concepts":[{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.8711000084877014},{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.8356000185012817},{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.7592999935150146},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.6216999888420105},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6051999926567078},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.5672000050544739},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.46540001034736633},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.429500013589859},{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.4059999883174896},{"id":"https://openalex.org/C81089528","wikidata":"https://www.wikidata.org/wiki/Q5134986","display_name":"clone (Java method)","level":3,"score":0.38659998774528503},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37439998984336853},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3327000141143799},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3264999985694885},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.3239000141620636},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.3028999865055084},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.2721000015735626}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.6084/m9.figshare.5284","doi":"https://doi.org/10.6084/m9.figshare.5284","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.5284","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Image"},{"id":"doi:10.6084/m9.figshare.5284.v1","doi":"https://doi.org/10.6084/m9.figshare.5284.v1","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.5284.v1","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Image"},{"id":"mag:2425831749","doi":null,"is_oa":false,"landing_page_url":"https://figshare.com/articles/Reduction_of_background_by_the_expression_of_tTS/5284","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.6084/m9.figshare.5284","doi":"https://doi.org/10.6084/m9.figshare.5284","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.5284","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Image"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.46810001134872437,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W6977419434","https://openalex.org/W6958473652","https://openalex.org/W2134104386","https://openalex.org/W6976957218","https://openalex.org/W1536734151","https://openalex.org/W1967223506","https://openalex.org/W1993980992","https://openalex.org/W2077183113","https://openalex.org/W3115076188","https://openalex.org/W2377575642"],"abstract_inverted_index":{"Copyright":[0],"information:Taken":[1],"from":[2],"\"Stringent":[3],"doxycycline-dependent":[4],"control":[5],"of":[6,74,101,133,139,152,154,168,174,207,212],"gene":[7],"activities":[8,132],"using":[9],"an":[10],"episomal":[11],"one-vector":[12],"system\"Nucleic":[13],"Acids":[14],"Research":[15],"2005;33(16):e137-e137.Published":[16],"online":[17],"7":[18],"Sep":[19],"2005PMCID:PMC1201338.\u00a9":[20],"The":[21,70,130,147],"Author":[22],"2005.":[23],"Published":[24],"by":[25,45],"Oxford":[26],"University":[27],"Press.":[28],"All":[29],"rights":[30],"reserved":[31],"()":[32],"BJAB":[33,86,183],"cells":[34,76,135,158],"(10)":[35],"were":[36,48,89,110,118],"transfected":[37,90],"with":[38,53,91,112],"10":[39],"\u03bcg":[40],"pRT-1":[41,92],"and":[42,60,83,103,117,122,136,156],"pRTS-1":[43,95],"DNA":[44],"electroporation.":[46],"Cells":[47,109],"left":[49,115,145],"untreated":[50,75,116],"or":[51,94,114],"treated":[52,111],"1":[54],"\u03bcg/ml":[55],"Dox":[56,113,128],"immediately":[57],"after":[58,67,107,127],"electroporation":[59],"luciferase":[61,121,131],"activity":[62,164],"measured":[63],"in":[64,98,143,149,165,180,204],"cell":[65,87,184,189,201],"extracts":[66],"48":[68,125],"h.":[69],"relative":[71],"light":[72],"units":[73],"are":[77,141,159,210],"given":[78],"above":[79],"the":[80,99,137,144,150,162,166,172,181,197,200],"columns.":[81],"(":[82],")":[84],"Three":[85],"lines":[88,202],"(B)":[93],"(C),":[96],"expanded":[97],"absence":[100,167],"Dox,":[102],"analysed":[104,119],"\u223c2":[105],"months":[106],"transfection.":[108],"for":[120],"eGFP":[123,175],"expression":[124],"h":[126],"addition.":[129],"uninduced":[134],"factor":[138,151],"inducibility":[140,153],"presented":[142],"panels.":[146],"differences":[148],"pRT-1-":[155],"pRTS-1-transfected":[157],"due":[160,195],"to":[161,196],"background":[163],"Dox.":[169],"Note":[170],"that":[171,199],"pattern":[173],"induction":[176],"is":[177,192],"not":[178,211],"uniform":[179],"different":[182],"lines,":[185],"even":[186],"within":[187],"one":[188],"line.":[190],"This":[191],"most":[193],"probably":[194],"fact":[198],"arising":[203],"individual":[205],"wells":[206],"96-well":[208],"plates":[209],"clonal":[213],"origin.":[214]},"counts_by_year":[],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2025-10-10T00:00:00"}