{"id":"https://openalex.org/W6958796123","doi":"https://doi.org/10.6084/m9.figshare.50197","title":"Western blot (WB) reacted with anti-phospho-ERK after treatment with FGF2 for 0, 5, 10 min (lanes 1\u20133) and after inhibitors LY294002 (to block PI3K), U0126 (to block MEK), and Bis I and G\u00f66983 (to block PKC) (lanes 4\u20137, respectively)","display_name":"Western blot (WB) reacted with anti-phospho-ERK after treatment with FGF2 for 0, 5, 10 min (lanes 1\u20133) and after inhibitors LY294002 (to block PI3K), U0126 (to block MEK), and Bis I and G\u00f66983 (to block PKC) (lanes 4\u20137, respectively)","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W6958796123","doi":"https://doi.org/10.6084/m9.figshare.50197","mag":"2546030893"},"language":"en","primary_location":{"id":"doi:10.6084/m9.figshare.50197","doi":"https://doi.org/10.6084/m9.figshare.50197","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.50197","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Image"},"volume":null,"type":"other","indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.6084/m9.figshare.50197","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Langford, Dianne","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Langford, Dianne","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006900974","display_name":"Hurford Rosemary","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hurford, Rosemary","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Hashimoto, Makoto","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hashimoto, Makoto","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081279614","display_name":"Murat H. Digicaylioglu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Digicaylioglu, Murat","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"Masliah, Eliezer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Masliah, Eliezer","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.49219998717308044,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.49219998717308044,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.1639000028371811,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11591","display_name":"NF-\u03baB Signaling Pathways","score":0.04320000112056732,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fgf2","display_name":"FGF2","score":0.9369999766349792},{"id":"https://openalex.org/keywords/erk1-2-phosphorylation","display_name":"ERK1/2 phosphorylation","score":0.7599999904632568},{"id":"https://openalex.org/keywords/pi3k-signaling","display_name":"PI3K signaling","score":0.6779999732971191},{"id":"https://openalex.org/keywords/mek","display_name":"MEK","score":0.8069999814033508},{"id":"https://openalex.org/keywords/protein-kinase-c","display_name":"protein kinase C","score":0.8140000104904175},{"id":"https://openalex.org/keywords/gsk-3beta","display_name":"GSK3\u03b2","score":0.8709999918937683},{"id":"https://openalex.org/keywords/signaling-crosstalk","display_name":"signaling crosstalk","score":0.9589999914169312},{"id":"https://openalex.org/keywords/endothelial-cells","display_name":"endothelial cells","score":0.9419999718666077},{"id":"https://openalex.org/keywords/hiv-gp120","display_name":"HIV gp120","score":0.9150000214576721},{"id":"https://openalex.org/keywords/neuroprotection","display_name":"neuroprotection","score":0.527999997138977}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7221999764442444},{"id":"https://openalex.org/C2777210771","wikidata":"https://www.wikidata.org/wiki/Q4927124","display_name":"Block (permutation group theory)","level":2,"score":0.6363000273704529},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.5586000084877014},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.46810001134872437},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.46399998664855957},{"id":"https://openalex.org/C57074206","wikidata":"https://www.wikidata.org/wiki/Q14197552","display_name":"MAPK/ERK pathway","level":3,"score":0.4163999855518341},{"id":"https://openalex.org/C2776912716","wikidata":"https://www.wikidata.org/wiki/Q4042503","display_name":"LY294002","level":4,"score":0.412200003862381},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.39329999685287476},{"id":"https://openalex.org/C85265743","wikidata":"https://www.wikidata.org/wiki/Q885458","display_name":"Blot","level":3,"score":0.3700999915599823},{"id":"https://openalex.org/C2779057293","wikidata":"https://www.wikidata.org/wiki/Q6543058","display_name":"Licensee","level":3,"score":0.3610999882221222},{"id":"https://openalex.org/C169822122","wikidata":"https://www.wikidata.org/wiki/Q230187","display_name":"Crosstalk","level":2,"score":0.35269999504089355},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.3497999906539917},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3402000069618225},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.304500013589859},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.27559998631477356},{"id":"https://openalex.org/C2778468042","wikidata":"https://www.wikidata.org/wiki/Q273976","display_name":"Blockade","level":3,"score":0.2689000070095062}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.6084/m9.figshare.50197","doi":"https://doi.org/10.6084/m9.figshare.50197","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.50197","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Image"},{"id":"doi:10.6084/m9.figshare.50197.v1","doi":"https://doi.org/10.6084/m9.figshare.50197.v1","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.50197.v1","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Image"},{"id":"mag:2546030893","doi":null,"is_oa":false,"landing_page_url":"https://figshare.com/articles/Western_blot_WB_reacted_with_anti_phospho_ERK_after_treatment_with_FGF2_for_0_5_10_min_lanes_1_3_and_after_inhibitors_LY294002_to_block_PI3K_U0126_to_block_MEK_and_Bis_I_and_G_6983_to_block_PKC_lanes_4_7_respectively_/50197","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.6084/m9.figshare.50197","doi":"https://doi.org/10.6084/m9.figshare.50197","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.50197","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Image"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.766700029373169}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W6902505454","https://openalex.org/W6902084846","https://openalex.org/W6939634552","https://openalex.org/W2032909215","https://openalex.org/W1900874372","https://openalex.org/W7161077350","https://openalex.org/W2006633782","https://openalex.org/W2186259898","https://openalex.org/W2571825415","https://openalex.org/W2059489998"],"abstract_inverted_index":{"Copyright":[0],"information:Taken":[1],"from":[2,11],"\"Signalling":[3],"crosstalk":[4],"in":[5,52],"FGF2-mediated":[6],"protection":[7],"of":[8],"endothelial":[9],"cells":[10],"HIV-gp120\"BMC":[12],"Neuroscience":[13],"2005;6():8-8.Published":[14],"online":[15],"2":[16],"Feb":[17],"2005PMCID:PMC549045.Copyright":[18],"\u00a9":[19],"2005":[20],"Langford":[21],"et":[22],"al;":[23],"licensee":[24],"BioMed":[25],"Central":[26],"Ltd.":[27],"WB":[28,34,38,42,49],"reacted":[29,35,39,43],"with":[30,36,40,44,60],"anti-total":[31],"ERK":[32],"antibody.":[33,46],"anti-phospho-GSK3\u03b2.":[37],"anti-GSK3\u03b2.":[41],"anti-PI3K":[45],"The":[47],"same":[48],"was":[50],"used":[51],"each":[53],"experiment":[54],"after":[55],"stripping,":[56],"reblocking":[57],"and":[58],"incubating":[59],"new":[61],"antibodies":[62],"for":[63],"the":[64],"given":[65],"protein.":[66]},"counts_by_year":[],"updated_date":"2026-10-13T10:08:12.033832","created_date":"2025-10-10T00:00:00"}