{"id":"https://openalex.org/W6889870204","doi":"https://doi.org/10.3204/pubdb-2025-01448","title":"Identification and Protein Engineering of Galactosidases for the Conversion of Blood Type B to Blood Type O","display_name":"Identification and Protein Engineering of Galactosidases for the Conversion of Blood Type B to Blood Type O","publication_year":2025,"publication_date":"2025-01-01","ids":{"openalex":"https://openalex.org/W6889870204","doi":"https://doi.org/10.3204/pubdb-2025-01448"},"language":"en","primary_location":{"id":"doi:10.3204/pubdb-2025-01448","doi":"https://doi.org/10.3204/pubdb-2025-01448","is_oa":true,"landing_page_url":"https://doi.org/10.3204/pubdb-2025-01448","pdf_url":null,"source":{"id":"https://openalex.org/S154285657","display_name":"ChemBioChem","issn_l":"1439-4227","issn":["1439-4227","1439-7633"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},"volume":null,"type":"article","indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://doi.org/10.3204/pubdb-2025-01448","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102002874","display_name":"Christina M\u00f6ller","orcid":"https://orcid.org/0000-0002-8286-1589","observed_orcids":["https://orcid.org/0000-0002-8286-1589"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M\u00f6ller, Christina","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-8286-1589","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031922781","display_name":"Henrik Terholsen","orcid":"https://orcid.org/0000-0001-9625-8177","observed_orcids":["https://orcid.org/0000-0001-9625-8177"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Terholsen, Henrik","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-9625-8177","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114501366","display_name":"Ole Schm\u00f6ker","orcid":"https://orcid.org/0009-0005-4754-8246","observed_orcids":["https://orcid.org/0009-0005-4754-8246"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Schm\u00f6ker, Ole","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"L\u00ea, Thi Linh Anne","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L\u00ea, Thi Linh Anne","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Wesche, Jan","orcid":"https://orcid.org/0000-0001-5025-7355","observed_orcids":["https://orcid.org/0000-0001-5025-7355"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wesche, Jan","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-5025-7355","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5116451046","display_name":"Paula Schmiade","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Schmiade, Paula","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5116451047","display_name":"Esther Eppendorfer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Eppendorfer, Esther","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5094335214","display_name":"Niklas Rimkus","orcid":"https://orcid.org/0009-0002-4057-2057","observed_orcids":["https://orcid.org/0009-0002-4057-2057"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Rimkus, Niklas","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0009-0002-4057-2057","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057855187","display_name":"Britta Girbardt","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Girbardt, Britta","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079472462","display_name":"Dominique B\u00f6ttcher","orcid":"https://orcid.org/0000-0001-9981-014X","observed_orcids":["https://orcid.org/0000-0001-9981-014X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"B\u00f6ttcher, Dominique","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-9981-014X","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067113624","display_name":"Gottfried Julius Palm","orcid":"https://orcid.org/0000-0003-0329-0413","observed_orcids":["https://orcid.org/0000-0003-0329-0413"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Palm, Gottfried J.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028618630","display_name":"Jens Hoppen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hoppen, Jens","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033846293","display_name":"Michael Lammers","orcid":"https://orcid.org/0000-0003-4168-4640","observed_orcids":["https://orcid.org/0000-0003-4168-4640"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lammers, Michael","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Greinacher, Andreas","orcid":"https://orcid.org/0000-0001-8343-7336","observed_orcids":["https://orcid.org/0000-0001-8343-7336"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Greinacher, Andreas","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-8343-7336","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033504648","display_name":"Konstanze Aurich","orcid":"https://orcid.org/0000-0003-3365-0168","observed_orcids":["https://orcid.org/0000-0003-3365-0168"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Aurich, Konstanze","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"Bornscheuer, Uwe T.","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bornscheuer, Uwe T.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.24379694,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.27880001068115234,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.27880001068115234,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.15610000491142273,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11660","display_name":"Blood groups and transfusion","score":0.1453000009059906,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/b-antigen","display_name":"B antigen","score":0.5109999775886536},{"id":"https://openalex.org/keywords/h-antigen","display_name":"H antigen","score":0.949999988079071},{"id":"https://openalex.org/keywords/deglycosylation","display_name":"deglycosylation","score":0.7239999771118164},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.8379999995231628},{"id":"https://openalex.org/keywords/protein-engineering","display_name":"protein engineering","score":0.7720000147819519},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.8240000009536743}],"concepts":[{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6093000173568726},{"id":"https://openalex.org/C184113351","wikidata":"https://www.wikidata.org/wiki/Q55588","display_name":"Blood type (non-human)","level":3,"score":0.5339000225067139},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5238000154495239},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4871000051498413},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.48410001397132874},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.41620001196861267},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.40459999442100525},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.37310001254081726},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.36579999327659607},{"id":"https://openalex.org/C1621761","wikidata":"https://www.wikidata.org/wiki/Q7997549","display_name":"Whole blood","level":2,"score":0.35740000009536743},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3093999922275543},{"id":"https://openalex.org/C3020437955","wikidata":"https://www.wikidata.org/wiki/Q7873","display_name":"Human blood","level":2,"score":0.2978000044822693},{"id":"https://openalex.org/C2909699947","wikidata":"https://www.wikidata.org/wiki/Q183605","display_name":"Blood units","level":3,"score":0.29580000042915344},{"id":"https://openalex.org/C2777299769","wikidata":"https://www.wikidata.org/wiki/Q3707858","display_name":"Type (biology)","level":2,"score":0.29319998621940613},{"id":"https://openalex.org/C2993270971","wikidata":"https://www.wikidata.org/wiki/Q11068","display_name":"Blood supply","level":2,"score":0.273499995470047},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.2662000060081482},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.26579999923706055},{"id":"https://openalex.org/C2780014101","wikidata":"https://www.wikidata.org/wiki/Q183605","display_name":"Blood transfusion","level":2,"score":0.26489999890327454},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.2621000111103058},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.25209999084472656}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3204/pubdb-2025-01448","doi":"https://doi.org/10.3204/pubdb-2025-01448","is_oa":true,"landing_page_url":"https://doi.org/10.3204/pubdb-2025-01448","pdf_url":null,"source":{"id":"https://openalex.org/S154285657","display_name":"ChemBioChem","issn_l":"1439-4227","issn":["1439-4227","1439-7633"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":null,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.3204/pubdb-2025-01448","doi":"https://doi.org/10.3204/pubdb-2025-01448","is_oa":true,"landing_page_url":"https://doi.org/10.3204/pubdb-2025-01448","pdf_url":null,"source":{"id":"https://openalex.org/S154285657","display_name":"ChemBioChem","issn_l":"1439-4227","issn":["1439-4227","1439-7633"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},"sustainable_development_goals":[{"score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"The":[0,151],"supply":[1],"of":[2,23,26,34,75,126,139,153,169,184],"blood":[3,8,24,36,54,186],"products":[4,25],"such":[5],"as":[6],"red":[7],"cells":[9],"poses":[10],"a":[11],"challenge":[12],"due":[13],"to":[14,82,99,117,165],"rising":[15],"demand":[16],"and":[17,41,60,145],"declining":[18],"donor":[19],"numbers.":[20],"Careful":[21],"matching":[22],"different":[27],"types":[28,37],"is":[29,61],"required.":[30],"Only":[31],"type":[32],"O":[33,42],"the":[35,73,76,84,100,107,124,143,158,178,181],"A,":[38],"B,":[39],"AB":[40],"can":[43,55],"be":[44,56],"received":[45],"by":[46,79,128],"any":[47],"patient":[48],"without":[49],"transfusion":[50],"incompatibilities.":[51],"Therefore,":[52],"O-type":[53,88],"considered":[57],"\u201cuniversal":[58],"blood\u201d":[59],"especially":[62],"needed":[63],"in":[64,142,147],"emergency":[65],"situations.":[66],"In":[67],"this":[68],"study,":[69],"we":[70,92,134],"focused":[71],"on":[72],"conversion":[74],"B":[77,173],"antigen":[78,86,174],"enzymatic":[80],"deglycosylation":[81],"generate":[83],"H":[85],"determining":[87],"blood.":[89,189],"For":[90],"this,":[91],"characterized":[93],"several":[94],"previously":[95,118],"unstudied":[96],"\u03b1-1,3-galactosidases":[97],"belonging":[98],"GH110":[101],"family.":[102],"Our":[103],"findings":[104],"revealed":[105],"that":[106],"\u03b1-1,3-galactosidase":[108],"from":[109,187],"Pedobacter":[110],"panaciterrae":[111],"(PpaGal)":[112],"exhibits":[113],"superior":[114],"efficiency":[115],"compared":[116],"described":[119],"galactosidases.":[120],"We":[121],"further":[122,167],"increased":[123],"activity":[125],"PpaGal":[127,170],"2.5-fold":[129],"using":[130],"site-directed":[131],"mutagenesis.":[132],"Moreover,":[133],"solved":[135,159],"two":[136],"crystal":[137,160],"structures":[138,161],"PpaGal,":[140],"one":[141],"apo-state":[144],"another":[146],"complex":[148],"with":[149,157],"d-galactose.":[150],"combination":[152],"our":[154],"mutagenesis":[155],"study":[156],"provides":[162],"valuable":[163],"information":[164],"guide":[166],"optimization":[168],"or":[171],"other":[172],"converting":[175],"enzymes":[176],"paving":[177],"way":[179],"for":[180],"easier":[182],"production":[183],"universal":[185],"B-type":[188]},"counts_by_year":[],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2025-10-10T00:00:00"}
