{"id":"https://openalex.org/W6889841218","doi":"https://doi.org/10.3204/pubdb-2025-01440","title":"Activity Regulation of a Glutamine Amidotransferase Bienzyme Complex by Substrate-Induced Subunit Interface Expansion","display_name":"Activity Regulation of a Glutamine Amidotransferase Bienzyme Complex by Substrate-Induced Subunit Interface Expansion","publication_year":2025,"publication_date":"2025-01-01","ids":{"openalex":"https://openalex.org/W6889841218","doi":"https://doi.org/10.3204/pubdb-2025-01440"},"language":"en","primary_location":{"id":"doi:10.3204/pubdb-2025-01440","doi":"https://doi.org/10.3204/pubdb-2025-01440","is_oa":true,"landing_page_url":"https://doi.org/10.3204/pubdb-2025-01440","pdf_url":null,"source":{"id":"https://openalex.org/S4306400383","display_name":"DESY Publication Database (PUBDB) (Deutsches Elektronen-Synchrotron)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801876189","host_organization_name":"Deutsches Elektronen-Synchrotron DESY","host_organization_lineage":["https://openalex.org/I2801876189"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},"volume":null,"type":"article","indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.3204/pubdb-2025-01440","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038462673","display_name":"Franziska Jasmin Funke","orcid":"https://orcid.org/0009-0005-7087-9334","observed_orcids":["https://orcid.org/0009-0005-7087-9334"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Funke, Franziska Jasmin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060930930","display_name":"Sandra Schlee","orcid":"https://orcid.org/0009-0005-7728-9134","observed_orcids":["https://orcid.org/0009-0005-7728-9134"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Schlee, Sandra","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065240386","display_name":"Isabel Bento","orcid":"https://orcid.org/0000-0003-3801-4929","observed_orcids":["https://orcid.org/0000-0003-3801-4929"]},"institutions":[{"id":"https://openalex.org/I4210138560","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/03mstc592","country_code":"DE","type":"government","lineage":["https://openalex.org/I4210138560"]},{"id":"https://openalex.org/I4210153543","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/050589e39","country_code":"DE","type":"government","lineage":["https://openalex.org/I4210138560","https://openalex.org/I4210153543"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Bento, Isabel","raw_affiliation_strings":["European Molecular Biology Laboratory"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"European Molecular Biology Laboratory","institution_ids":["https://openalex.org/I4210138560","https://openalex.org/I4210153543"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040780127","display_name":"Gleb P. Bourenkov","orcid":"https://orcid.org/0000-0002-2617-5920","observed_orcids":["https://orcid.org/0000-0002-2617-5920"]},"institutions":[{"id":"https://openalex.org/I4210138560","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/03mstc592","country_code":"DE","type":"government","lineage":["https://openalex.org/I4210138560"]},{"id":"https://openalex.org/I4210153543","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/050589e39","country_code":"DE","type":"government","lineage":["https://openalex.org/I4210138560","https://openalex.org/I4210153543"]},{"id":"https://openalex.org/I910829146","display_name":"European X-Ray Free-Electron Laser","ror":"https://ror.org/01wp2jz98","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I1305996414","https://openalex.org/I160013858","https://openalex.org/I2738703131","https://openalex.org/I2800235667","https://openalex.org/I2801876189","https://openalex.org/I2802499594","https://openalex.org/I4210087105","https://openalex.org/I4210097150","https://openalex.org/I4210124562","https://openalex.org/I4210132216","https://openalex.org/I4210140065","https://openalex.org/I4210142071","https://openalex.org/I4210155236","https://openalex.org/I4387152914","https://openalex.org/I4388482678","https://openalex.org/I910829146"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Bourenkov, Gleb","raw_affiliation_strings":["European Molecular Biology Laboratory","European XFEL"],"raw_orcid":"https://orcid.org/0000-0002-2617-5920","affiliations":[{"raw_affiliation_string":"European Molecular Biology Laboratory","institution_ids":["https://openalex.org/I4210138560","https://openalex.org/I4210153543"]},{"raw_affiliation_string":"European XFEL","institution_ids":["https://openalex.org/I910829146"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002529505","display_name":"Reinhard Sterner","orcid":"https://orcid.org/0000-0001-8177-8460","observed_orcids":["https://orcid.org/0000-0001-8177-8460"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sterner, Reinhard","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5088714014","display_name":"Matthias Wilmanns","orcid":"https://orcid.org/0000-0002-4643-5435","observed_orcids":["https://orcid.org/0000-0002-4643-5435"]},"institutions":[{"id":"https://openalex.org/I4210138560","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/03mstc592","country_code":"DE","type":"government","lineage":["https://openalex.org/I4210138560"]},{"id":"https://openalex.org/I4210153543","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/050589e39","country_code":"DE","type":"government","lineage":["https://openalex.org/I4210138560","https://openalex.org/I4210153543"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Wilmanns, Matthias","raw_affiliation_strings":["European Molecular Biology Laboratory"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"European Molecular Biology Laboratory","institution_ids":["https://openalex.org/I4210138560","https://openalex.org/I4210153543"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.18647476,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":true,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.43849998712539673,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.43849998712539673,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.25270000100135803,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.2371000051498413,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glutamine-amidotransferase","display_name":"glutamine amidotransferase","score":0.9800000190734863},{"id":"https://openalex.org/keywords/multienzyme-complex","display_name":"multienzyme complex","score":0.8370000123977661},{"id":"https://openalex.org/keywords/glutaminase","display_name":"glutaminase","score":0.8080000281333923},{"id":"https://openalex.org/keywords/ammonia-transport","display_name":"ammonia transport","score":0.6539999842643738},{"id":"https://openalex.org/keywords/folate-biosynthesis","display_name":"folate biosynthesis","score":0.75},{"id":"https://openalex.org/keywords/enzyme-regulation","display_name":"enzyme regulation","score":0.45399999618530273},{"id":"https://openalex.org/keywords/protein-tunnels","display_name":"protein tunnels","score":0.3179999887943268},{"id":"https://openalex.org/keywords/glutamine-synthetase","display_name":"glutamine synthetase","score":0.6370000243186951}],"concepts":[{"id":"https://openalex.org/C75972863","wikidata":"https://www.wikidata.org/wiki/Q5572442","display_name":"Glutamine amidotransferase","level":4,"score":0.9592999815940857},{"id":"https://openalex.org/C109549099","wikidata":"https://www.wikidata.org/wiki/Q24773687","display_name":"Glutaminase","level":4,"score":0.8090000152587891},{"id":"https://openalex.org/C112243037","wikidata":"https://www.wikidata.org/wiki/Q22327117","display_name":"ATP synthase","level":3,"score":0.6816999912261963},{"id":"https://openalex.org/C2779349466","wikidata":"https://www.wikidata.org/wiki/Q181619","display_name":"Glutamine","level":3,"score":0.6545000076293945},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6367999911308289},{"id":"https://openalex.org/C191771488","wikidata":"https://www.wikidata.org/wiki/Q3261275","display_name":"Glutamate synthase","level":5,"score":0.6273999810218811},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.5824000239372253},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5701000094413757},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.4147999882698059},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.39959999918937683},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.3865000009536743},{"id":"https://openalex.org/C200159599","wikidata":"https://www.wikidata.org/wiki/Q4893563","display_name":"Pyrimidine metabolism","level":4,"score":0.35760000348091125},{"id":"https://openalex.org/C2776376580","wikidata":"https://www.wikidata.org/wiki/Q407355","display_name":"Transferase","level":3,"score":0.3100999891757965},{"id":"https://openalex.org/C2779289688","wikidata":"https://www.wikidata.org/wiki/Q418306","display_name":"Glutamine synthetase","level":4,"score":0.29190000891685486},{"id":"https://openalex.org/C119120687","wikidata":"https://www.wikidata.org/wiki/Q4654890","display_name":"ATP synthase gamma subunit","level":5,"score":0.28999999165534973},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.28209999203681946},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.2720000147819519},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2660999894142151},{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.2554999887943268}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3204/pubdb-2025-01440","doi":"https://doi.org/10.3204/pubdb-2025-01440","is_oa":true,"landing_page_url":"https://doi.org/10.3204/pubdb-2025-01440","pdf_url":null,"source":{"id":"https://openalex.org/S4306400383","display_name":"DESY Publication Database (PUBDB) (Deutsches Elektronen-Synchrotron)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801876189","host_organization_name":"Deutsches Elektronen-Synchrotron DESY","host_organization_lineage":["https://openalex.org/I2801876189"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":null,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.3204/pubdb-2025-01440","doi":"https://doi.org/10.3204/pubdb-2025-01440","is_oa":true,"landing_page_url":"https://doi.org/10.3204/pubdb-2025-01440","pdf_url":null,"source":{"id":"https://openalex.org/S4306400383","display_name":"DESY Publication Database (PUBDB) (Deutsches Elektronen-Synchrotron)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801876189","host_organization_name":"Deutsches Elektronen-Synchrotron DESY","host_organization_lineage":["https://openalex.org/I2801876189"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/6","display_name":"Clean water and sanitation","score":0.7107313275337219}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Glutamine":[0],"amidotransferases":[1],"are":[2],"multienzyme":[3,149],"machineries":[4],"in":[5,71,81,132],"which":[6],"reactive":[7],"ammonia":[8,121],"is":[9,38,50],"generated":[10],"by":[11,98],"a":[12,18,23,39,75,94,140],"glutaminase":[13,65,109],"and":[14,115],"then":[15],"transferred":[16],"through":[17],"sequestered":[19,113],"protein":[20],"tunnel":[21],"to":[22,93,122],"synthase":[24,54,69,124],"active":[25,110,125],"site":[26,111],"for":[27,41,112,143],"incorporation":[28],"into":[29],"diverse":[30],"metabolites.":[31],"To":[32],"avoid":[33],"wasteful":[34],"metabolite":[35],"consumption,":[36],"there":[37],"requirement":[40],"synchronized":[42],"catalysis,":[43,85],"but":[44],"any":[45],"generally":[46],"applicable":[47],"mechanistic":[48],"insight":[49],"still":[51],"lacking.":[52],"As":[53],"activity":[55],"depends":[56],"on":[57],"glutamine":[58,91,134],"turnover,":[59],"we":[60,86],"investigated":[61],"possible":[62],"mechanisms":[63],"controlling":[64],"catalysis":[66,114,145],"using":[67],"aminodeoxychorismate":[68],"involved":[70],"folate":[72],"biosynthesis":[73],"as":[74],"model.":[76],"By":[77],"analyzing":[78],"this":[79,148],"system":[80],"distinct":[82],"states":[83],"of":[84,100,119,129,147],"found":[87],"that":[88],"incubation":[89],"with":[90],"leads":[92],"subunit":[95],"interface":[96],"expansion":[97],"one-third":[99],"its":[101],"original":[102],"area.":[103],"These":[104],"changes":[105],"completely":[106],"enclose":[107],"the":[108,116,123,144],"subsequent":[117],"transport":[118],"volatile":[120],"site.":[126],"In":[127],"view":[128],"similar":[130],"rearrangements":[131],"other":[133],"amidotransferases,":[135],"our":[136],"observations":[137],"may":[138],"provide":[139],"general":[141],"mechanism":[142],"synchronization":[146],"family.":[150]},"counts_by_year":[],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2025-10-10T00:00:00"}