{"id":"https://openalex.org/W67820503","doi":null,"title":"The development of Lactococcus lactis as an antimicrobial agent","display_name":"The development of Lactococcus lactis as an antimicrobial agent","publication_year":2010,"publication_date":"2010-01-01","ids":{"openalex":"https://openalex.org/W67820503","mag":"67820503"},"language":"en","primary_location":{"id":"mag:67820503","doi":null,"is_oa":false,"landing_page_url":"https://eprints.qut.edu.au/39143/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086857977","display_name":"Yu Pei Tan","orcid":"https://orcid.org/0000-0002-4565-6148","observed_orcids":["https://orcid.org/0000-0002-4565-6148"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Yu Pei Tan","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-4565-6148","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5086857977"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.9463000297546387,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.9463000297546387,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.019300000742077827,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12996","display_name":"Bacterial Cancer Therapy","score":0.01590000092983246,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lactococcus-lactis","display_name":"Lactococcus lactis","score":0.9900000095367432},{"id":"https://openalex.org/keywords/antimicrobial-proteins","display_name":"antimicrobial proteins","score":0.6700000166893005},{"id":"https://openalex.org/keywords/lysostaphin","display_name":"lysostaphin","score":0.9070000052452087},{"id":"https://openalex.org/keywords/limosilactobacillus-reuteri","display_name":"Lactobacillus reuteri","score":0.9309999942779541},{"id":"https://openalex.org/keywords/staphylococcus-aureus","display_name":"Staphylococcus aureus","score":0.9049999713897705},{"id":"https://openalex.org/keywords/bacteriolytic-enzymes","display_name":"bacteriolytic enzymes","score":0.5440000295639038},{"id":"https://openalex.org/keywords/fibronectin-binding","display_name":"fibronectin binding","score":0.7269999980926514},{"id":"https://openalex.org/keywords/extracellular-matrix-adhesion","display_name":"extracellular matrix adhesion","score":0.6990000009536743},{"id":"https://openalex.org/keywords/heterologous-protein-secretion","display_name":"heterologous protein secretion","score":0.7279999852180481},{"id":"https://openalex.org/keywords/transposon-mutagenesis","display_name":"transposon mutagenesis","score":0.8450000286102295},{"id":"https://openalex.org/keywords/peptidoglycan-synthesis","display_name":"peptidoglycan biosynthesis","score":0.8970000147819519}],"concepts":[{"id":"https://openalex.org/C2780011460","wikidata":"https://www.wikidata.org/wiki/Q133598","display_name":"Lactococcus lactis","level":4,"score":0.9162288904190063},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.7312893271446228},{"id":"https://openalex.org/C2778673385","wikidata":"https://www.wikidata.org/wiki/Q5874320","display_name":"Lysostaphin","level":4,"score":0.7112732529640198},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5832251310348511},{"id":"https://openalex.org/C2780368718","wikidata":"https://www.wikidata.org/wiki/Q7623352","display_name":"Streptococcus gordonii","level":4,"score":0.5790173411369324},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.5023164749145508},{"id":"https://openalex.org/C86492073","wikidata":"https://www.wikidata.org/wiki/Q413766","display_name":"Fibronectin","level":3,"score":0.500260591506958},{"id":"https://openalex.org/C2779489039","wikidata":"https://www.wikidata.org/wiki/Q188121","display_name":"Staphylococcus aureus","level":3,"score":0.48872923851013184},{"id":"https://openalex.org/C4937899","wikidata":"https://www.wikidata.org/wiki/Q68541106","display_name":"Antimicrobial","level":2,"score":0.46492862701416016},{"id":"https://openalex.org/C2775898472","wikidata":"https://www.wikidata.org/wiki/Q597078","display_name":"Lactobacillus reuteri","level":4,"score":0.45669347047805786},{"id":"https://openalex.org/C2780913471","wikidata":"https://www.wikidata.org/wiki/Q134274","display_name":"Lactococcus","level":5,"score":0.4566364586353302},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.45433172583580017},{"id":"https://openalex.org/C2780206646","wikidata":"https://www.wikidata.org/wiki/Q1061596","display_name":"Lactobacillus","level":3,"score":0.4329524040222168},{"id":"https://openalex.org/C540938839","wikidata":"https://www.wikidata.org/wiki/Q1201508","display_name":"Antimicrobial peptides","level":3,"score":0.4237530529499054},{"id":"https://openalex.org/C115574571","wikidata":"https://www.wikidata.org/wiki/Q183119","display_name":"Bacteriocin","level":3,"score":0.41345033049583435},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2879895567893982},{"id":"https://openalex.org/C2775920511","wikidata":"https://www.wikidata.org/wiki/Q161249","display_name":"Lactic acid","level":3,"score":0.2787330150604248},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.23142677545547485},{"id":"https://openalex.org/C2778663903","wikidata":"https://www.wikidata.org/wiki/Q140018","display_name":"Streptococcaceae","level":3,"score":0.1723025143146515},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:67820503","doi":null,"is_oa":false,"landing_page_url":"https://eprints.qut.edu.au/39143/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.7246000170707703}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":277,"referenced_works":["https://openalex.org/W3926522","https://openalex.org/W14156690","https://openalex.org/W153858581","https://openalex.org/W177077418","https://openalex.org/W198219662","https://openalex.org/W205578041","https://openalex.org/W1481121752","https://openalex.org/W1485776927","https://openalex.org/W1486214911","https://openalex.org/W1486497947","https://openalex.org/W1492250056","https://openalex.org/W1493583453","https://openalex.org/W1495514947","https://openalex.org/W1502559266","https://openalex.org/W1508801011","https://openalex.org/W1509140125","https://openalex.org/W1524870033","https://openalex.org/W1533592790","https://openalex.org/W1537205292","https://openalex.org/W1541177965","https://openalex.org/W1542608577","https://openalex.org/W1551260639","https://openalex.org/W1558230394","https://openalex.org/W1558571934","https://openalex.org/W1571150808","https://openalex.org/W1574575997","https://openalex.org/W1574694626","https://openalex.org/W1574840625","https://openalex.org/W1575140104","https://openalex.org/W1575450081","https://openalex.org/W1586338532","https://openalex.org/W1601256941","https://openalex.org/W1650320312","https://openalex.org/W1676291112","https://openalex.org/W1679411437","https://openalex.org/W1711823888","https://openalex.org/W1741443434","https://openalex.org/W1748166210","https://openalex.org/W1784096314","https://openalex.org/W1790851221","https://openalex.org/W1821656608","https://openalex.org/W1852844868","https://openalex.org/W1869325074","https://openalex.org/W1876098200","https://openalex.org/W1900392249","https://openalex.org/W1953756384","https://openalex.org/W1960450979","https://openalex.org/W1962306195","https://openalex.org/W1966070420","https://openalex.org/W1966436360","https://openalex.org/W1967889828","https://openalex.org/W1968232055","https://openalex.org/W1968339880","https://openalex.org/W1968582377","https://openalex.org/W1969788984","https://openalex.org/W1970532969","https://openalex.org/W1970633222","https://openalex.org/W1970763155","https://openalex.org/W1971120607","https://openalex.org/W1971470851","https://openalex.org/W1971668229","https://openalex.org/W1973259178","https://openalex.org/W1975288774","https://openalex.org/W1975521395","https://openalex.org/W1975900478","https://openalex.org/W1976953689","https://openalex.org/W1977881285","https://openalex.org/W1979963523","https://openalex.org/W1981765089","https://openalex.org/W1982994563","https://openalex.org/W1983966505","https://openalex.org/W1989369170","https://openalex.org/W1989787472","https://openalex.org/W1992498209","https://openalex.org/W1994895410","https://openalex.org/W1995105203","https://openalex.org/W1997265343","https://openalex.org/W1997347195","https://openalex.org/W1997370001","https://openalex.org/W1997433152","https://openalex.org/W1998075136","https://openalex.org/W1999870427","https://openalex.org/W1999965330","https://openalex.org/W2000182766","https://openalex.org/W2000441147","https://openalex.org/W2002560537","https://openalex.org/W2002785901","https://openalex.org/W2004821013","https://openalex.org/W2007995269","https://openalex.org/W2008898532","https://openalex.org/W2009221323","https://openalex.org/W2009296652","https://openalex.org/W2013682169","https://openalex.org/W2014587515","https://openalex.org/W2014621628","https://openalex.org/W2018062498","https://openalex.org/W2019147544","https://openalex.org/W2019352328","https://openalex.org/W2021501853","https://openalex.org/W2021701490","https://openalex.org/W2022124341","https://openalex.org/W2023002155","https://openalex.org/W2023039866","https://openalex.org/W2023490354","https://openalex.org/W2023518339","https://openalex.org/W2023846168","https://openalex.org/W2023890960","https://openalex.org/W2023907724","https://openalex.org/W2026158782","https://openalex.org/W2026838076","https://openalex.org/W2027657428","https://openalex.org/W2028093121","https://openalex.org/W2029112298","https://openalex.org/W2033575336","https://openalex.org/W2035728933","https://openalex.org/W2037834616","https://openalex.org/W2037862520","https://openalex.org/W2041268453","https://openalex.org/W2042035526","https://openalex.org/W2043904270","https://openalex.org/W2046002331","https://openalex.org/W2046389334","https://openalex.org/W2047491738","https://openalex.org/W2049323790","https://openalex.org/W2049955359","https://openalex.org/W2051025546","https://openalex.org/W2051250838","https://openalex.org/W2052291160","https://openalex.org/W2052580524","https://openalex.org/W2053353201","https://openalex.org/W2053718480","https://openalex.org/W2053876639","https://openalex.org/W2054257072","https://openalex.org/W2057910559","https://openalex.org/W2059438459","https://openalex.org/W2059825445","https://openalex.org/W2060102925","https://openalex.org/W2060988655","https://openalex.org/W2062417466","https://openalex.org/W2063671533","https://openalex.org/W2065129970","https://openalex.org/W2071578025","https://openalex.org/W2072604958","https://openalex.org/W2077010037","https://openalex.org/W2079568089","https://openalex.org/W2086308847","https://openalex.org/W2086542505","https://openalex.org/W2088745698","https://openalex.org/W2090081527","https://openalex.org/W2094184056","https://openalex.org/W2095067671","https://openalex.org/W2096299972","https://openalex.org/W2096354845","https://openalex.org/W2096447579","https://openalex.org/W2096714870","https://openalex.org/W2097706568","https://openalex.org/W2098112056","https://openalex.org/W2098637615","https://openalex.org/W2098686923","https://openalex.org/W2099836442","https://openalex.org/W2100824730","https://openalex.org/W2100826827","https://openalex.org/W2100882223","https://openalex.org/W2102672175","https://openalex.org/W2103667123","https://openalex.org/W2104351524","https://openalex.org/W2107749220","https://openalex.org/W2108708484","https://openalex.org/W2109105334","https://openalex.org/W2109621905","https://openalex.org/W2111086397","https://openalex.org/W2111113492","https://openalex.org/W2111564369","https://openalex.org/W2111752110","https://openalex.org/W2111785687","https://openalex.org/W2113645094","https://openalex.org/W2114000572","https://openalex.org/W2114567719","https://openalex.org/W2114589940","https://openalex.org/W2114644887","https://openalex.org/W2114869475","https://openalex.org/W2115965948","https://openalex.org/W2116959157","https://openalex.org/W2117901410","https://openalex.org/W2117919289","https://openalex.org/W2118206090","https://openalex.org/W2119237104","https://openalex.org/W2119243044","https://openalex.org/W2120994746","https://openalex.org/W2120994830","https://openalex.org/W2121138643","https://openalex.org/W2121637412","https://openalex.org/W2121659243","https://openalex.org/W2122254428","https://openalex.org/W2123300343","https://openalex.org/W2124903080","https://openalex.org/W2125116820","https://openalex.org/W2125121305","https://openalex.org/W2125920591","https://openalex.org/W2128007276","https://openalex.org/W2128207929","https://openalex.org/W2128965279","https://openalex.org/W2130485553","https://openalex.org/W2130577867","https://openalex.org/W2131439453","https://openalex.org/W2132119271","https://openalex.org/W2132364034","https://openalex.org/W2132552460","https://openalex.org/W2132596405","https://openalex.org/W2132644314","https://openalex.org/W2133260257","https://openalex.org/W2136266813","https://openalex.org/W2137517599","https://openalex.org/W2139906115","https://openalex.org/W2140544780","https://openalex.org/W2140901337","https://openalex.org/W2142340262","https://openalex.org/W2142441629","https://openalex.org/W2142965789","https://openalex.org/W2143376633","https://openalex.org/W2143570924","https://openalex.org/W2144005980","https://openalex.org/W2144758921","https://openalex.org/W2144801144","https://openalex.org/W2145683869","https://openalex.org/W2146052711","https://openalex.org/W2146359135","https://openalex.org/W2146396614","https://openalex.org/W2146465657","https://openalex.org/W2147805608","https://openalex.org/W2147921307","https://openalex.org/W2148337956","https://openalex.org/W2149186760","https://openalex.org/W2150296552","https://openalex.org/W2150358456","https://openalex.org/W2151106935","https://openalex.org/W2151172470","https://openalex.org/W2151757385","https://openalex.org/W2152264007","https://openalex.org/W2153038986","https://openalex.org/W2154066176","https://openalex.org/W2154659630","https://openalex.org/W2155436577","https://openalex.org/W2155864378","https://openalex.org/W2156133722","https://openalex.org/W2156783270","https://openalex.org/W2157778625","https://openalex.org/W2158433014","https://openalex.org/W2158810697","https://openalex.org/W2159294745","https://openalex.org/W2161137494","https://openalex.org/W2161194226","https://openalex.org/W2163480997","https://openalex.org/W2163836917","https://openalex.org/W2163842908","https://openalex.org/W2165256578","https://openalex.org/W2165547120","https://openalex.org/W2166873510","https://openalex.org/W2168225803","https://openalex.org/W2168355088","https://openalex.org/W2168772829","https://openalex.org/W2169510298","https://openalex.org/W2169784240","https://openalex.org/W2169790560","https://openalex.org/W2169908415","https://openalex.org/W2169920573","https://openalex.org/W2170530228","https://openalex.org/W2170783143","https://openalex.org/W2171076336","https://openalex.org/W2173603236","https://openalex.org/W2180747649","https://openalex.org/W2322769512","https://openalex.org/W2329427515","https://openalex.org/W2395035467","https://openalex.org/W2410537711","https://openalex.org/W2415991929","https://openalex.org/W2782303467"],"related_works":["https://openalex.org/W1592095376","https://openalex.org/W2145967310","https://openalex.org/W1989369170","https://openalex.org/W4210437639","https://openalex.org/W1559765718","https://openalex.org/W4366821600","https://openalex.org/W1976316617","https://openalex.org/W2089076311","https://openalex.org/W2146809179","https://openalex.org/W7095176536"],"abstract_inverted_index":{"Non-pathogenic":[0],"lactic":[1,95],"acid":[2,96],"bacteria":[3,8],"are":[4,32],"economically":[5],"important":[6],"Gram-positive":[7,166],"used":[9],"extensively":[10],"in":[11,275,294,314,324],"the":[12,27,39,71,79,82,93,111,130,165,184,188,204,208,213,221,229,233,255,260,291,302,347],"food":[13],"industry.":[14],"Due":[16],"to":[17,63,77,107,150,179,186,193,219,226,241,244,329,332,346,358],"their":[18],"\u201cgenerally":[19],"regarded":[20],"as":[21,36,141,352],"safe\u201d":[22],"status,":[23],"certain":[24],"species":[25],"from":[26,116],"genera":[28],"Lactobacillus":[29,117],"and":[30,41,109,120,127,133,307,356],"Lactococcus":[31,58,98],"also":[33,147,357],"considered":[34],"desirable":[35],"candidates":[37],"for":[38],"production":[40],"secretion":[42,84,134,256],"of":[43,73,81,87,125,190,207,223,232,257,262,301,335,342,349,361],"recombinant":[44,112],"proteins,":[45],"particular":[46],"those":[47],"with":[48,161,212,269],"therapeutic":[49],"applications.\r\n\r\nThe":[50],"hypothesis":[51],"examined":[52],"by":[53,92],"this":[54,74,246,343,350],"thesis":[55,75,247,344],"is":[56,157],"that":[57,203,253],"lactis":[59,99,103,174,210,235,363],"can":[60],"be":[61,64,330],"modified":[62,172],"an":[65,281,288,309,353],"effective":[66],"antimicrobial":[67,90,354],"agent.":[68],"Therefore,":[70],"aims":[72],"were":[76,105,176],"investigate":[78],"optimisation":[80],"expression,":[83],"and/or":[85],"activities":[86],"potential":[88],"heterologous":[89],"proteins":[91],"model":[94],"bacterium,":[97],"subsp.":[100],"cremoris":[101],"MG1363.\r\n\r\nL.":[102],"strains":[104,175],"engineered":[106],"express":[108],"secrete":[110],"CyuC":[113,126,137],"surface":[114],"protein":[115,123,285],"reuteri":[118],"BR11,":[119],"a":[121,142,158,299],"fusion":[122],"consisting":[124],"lysostaphin":[128,238,258,271,321,336],"using":[129],"Sep":[131],"promoter":[132],"signal.":[135],"has":[138,146,248],"been":[139,148],"characterised":[140],"cystine-binding":[143],"protein,":[144],"but":[145],"demonstrated":[149],"have":[151],"fibronectin":[152],"binding":[153],"activity.":[154],"Lysostaphin":[156],"bacteriolytic":[159],"enzyme":[160,289,312],"specific":[162,250],"activity":[163,272,322],"against":[164],"pathogen,":[167],"Staphylococcus":[168],"aureus.":[169],"These":[171],"L.":[173,209,234,362],"then":[177],"investigated":[178],"see":[180],"if":[181],"they":[182],"had":[183],"ability":[185],"inhibit":[187,220,242],"adhesion":[189,222,243],"S.":[191,224],"aureus":[192,225],"host":[194],"extracellular":[195],"matrix":[196],"(ECM)":[197],"proteins.":[198],"It":[200],"was":[201,217,239,327],"observed":[202,323],"cell":[205,230],"extracts":[206,231],"strain":[211,236],"vector":[214],"only":[215],"(pGhost9:ISS1)":[216],"able":[218,240],"fibronectin,":[227],"whilst":[228],"expressing":[237],"keratin.\r\n\r\nFinally,":[245],"identified":[249],"lactococcal":[251],"genes":[252,278],"affect":[254],"through":[259],"use":[261],"random":[263],"transposon":[264],"mutagenesis.":[265],"Ten":[267],"mutants":[268,326],"higher":[270,320,333],"contained":[273],"insertions":[274],"four":[276],"different":[277],"encoding:":[279],"(i)":[280],"uncharacterised":[282,310],"putative":[283,311],"transmembrane":[284],"(llmg_0609),":[286],"(ii)":[287],"catalysing":[290],"first":[292],"step":[293],"peptidoglycan":[295],"biosynthesis":[296,316],"(murA2),":[297],"(iii)":[298],"homolog":[300],"oxidative":[303],"defence":[304],"regulator":[305],"(trmA),":[306],"(iv)":[308],"involved":[313],"ubiquinone":[315],"(llmg_2148).":[317],"The":[319],"these":[325],"found":[328],"due":[331],"amounts":[334],"being":[337],"secreted.":[338],"\r\n\r\nThe":[340],"findings":[341],"contribute":[345],"development":[348],"organism":[351],"agent":[355],"our":[359],"understanding":[360],"genetics.":[364]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
