{"id":"https://openalex.org/W65265539","doi":"https://doi.org/10.1042/bj2050593","title":"Superoxide-forming NADPH oxidase preparation of pig polymorphonuclear leucocyte","display_name":"Superoxide-forming NADPH oxidase preparation of pig polymorphonuclear leucocyte","publication_year":1982,"publication_date":"1982-09-01","ids":{"openalex":"https://openalex.org/W65265539","doi":"https://doi.org/10.1042/bj2050593","mag":"65265539","pmid":"https://pubmed.ncbi.nlm.nih.gov/6293459","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1158526"},"language":"en","primary_location":{"id":"doi:10.1042/bj2050593","doi":"https://doi.org/10.1042/bj2050593","is_oa":false,"landing_page_url":"https://doi.org/10.1042/bj2050593","pdf_url":null,"source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1158526","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113451037","display_name":"H Wakeyama","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135598925","display_name":"Kyushu University","ror":"https://ror.org/00p4k0j84","country_code":"JP","type":"education","lineage":["https://openalex.org/I135598925"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"H Wakeyama","raw_affiliation_strings":["Department ofBiochemistry, Kyushu University School ofMedicine, Fukuoka 812, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofBiochemistry, Kyushu University School ofMedicine, Fukuoka 812, Japan","institution_ids":["https://openalex.org/I135598925"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088479379","display_name":"Koichiro Takeshige","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135598925","display_name":"Kyushu University","ror":"https://ror.org/00p4k0j84","country_code":"JP","type":"education","lineage":["https://openalex.org/I135598925"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"K Takeshige","raw_affiliation_strings":["Department ofBiochemistry, Kyushu University School ofMedicine, Fukuoka 812, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofBiochemistry, Kyushu University School ofMedicine, Fukuoka 812, Japan","institution_ids":["https://openalex.org/I135598925"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111083328","display_name":"R. Takayanagi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135598925","display_name":"Kyushu University","ror":"https://ror.org/00p4k0j84","country_code":"JP","type":"education","lineage":["https://openalex.org/I135598925"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"R Takayanagi","raw_affiliation_strings":["Department ofBiochemistry, Kyushu University School ofMedicine, Fukuoka 812, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofBiochemistry, Kyushu University School ofMedicine, Fukuoka 812, Japan","institution_ids":["https://openalex.org/I135598925"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110182066","display_name":"S Minakami","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135598925","display_name":"Kyushu University","ror":"https://ror.org/00p4k0j84","country_code":"JP","type":"education","lineage":["https://openalex.org/I135598925"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"S Minakami","raw_affiliation_strings":["Department ofBiochemistry, Kyushu University School ofMedicine, Fukuoka 812, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department ofBiochemistry, Kyushu University School ofMedicine, Fukuoka 812, Japan","institution_ids":["https://openalex.org/I135598925"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I135598925"],"apc_list":null,"apc_paid":null,"fwci":4.234,"has_fulltext":false,"cited_by_count":97,"citation_normalized_percentile":{"value":0.94608046,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"205","issue":"3","first_page":"593","last_page":"601"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11597","display_name":"Neutrophil, Myeloperoxidase and Oxidative Mechanisms","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11597","display_name":"Neutrophil, Myeloperoxidase and Oxidative Mechanisms","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.008799999952316284,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12356","display_name":"Bioactive Compounds and Antitumor Agents","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/3005","display_name":"Toxicology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nadph-oxidase","display_name":"NADPH oxidase","score":0.9919999837875366},{"id":"https://openalex.org/keywords/superoxide-production","display_name":"superoxide production","score":0.8479999899864197},{"id":"https://openalex.org/keywords/polymorphonuclear-leukocytes","display_name":"polymorphonuclear leukocytes","score":0.8999999761581421},{"id":"https://openalex.org/keywords/flavin","display_name":"flavin","score":0.3449999988079071},{"id":"https://openalex.org/keywords/fad","display_name":"FAD","score":0.8740000128746033},{"id":"https://openalex.org/keywords/nadph-oxidation","display_name":"NADPH oxidation","score":0.7179999947547913},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.652999997138977},{"id":"https://openalex.org/keywords/enzyme-stability","display_name":"enzyme stability","score":0.41600000858306885},{"id":"https://openalex.org/keywords/phagocytosis","display_name":"phagocytosis","score":0.6740000247955322}],"concepts":[{"id":"https://openalex.org/C2780795997","wikidata":"https://www.wikidata.org/wiki/Q413283","display_name":"Superoxide","level":3,"score":0.81308913230896},{"id":"https://openalex.org/C2779719074","wikidata":"https://www.wikidata.org/wiki/Q2038057","display_name":"NADPH oxidase","level":3,"score":0.6337600946426392},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6075080633163452},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5932500958442688},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.5160150527954102},{"id":"https://openalex.org/C2775838275","wikidata":"https://www.wikidata.org/wiki/Q410776","display_name":"Superoxide dismutase","level":3,"score":0.4826710522174835},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.47674477100372314},{"id":"https://openalex.org/C38485361","wikidata":"https://www.wikidata.org/wiki/Q576379","display_name":"Oxidase test","level":3,"score":0.4704180061817169},{"id":"https://openalex.org/C2778455166","wikidata":"https://www.wikidata.org/wiki/Q55076544","display_name":"Cyanide","level":2,"score":0.46402862668037415},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.10531002283096313},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.09963050484657288}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009247","descriptor_name":"NADH, NADPH Oxidoreductases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D009247","descriptor_name":"NADH, NADPH Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009504","descriptor_name":"Neutrophils","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D010100","descriptor_name":"Oxygen","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D010587","descriptor_name":"Phagocytosis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D011190","descriptor_name":"Potassium Cyanide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013347","descriptor_name":"Subcellular Fractions","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013481","descriptor_name":"Superoxides","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019255","descriptor_name":"NADPH Oxidases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1042/bj2050593","doi":"https://doi.org/10.1042/bj2050593","is_oa":false,"landing_page_url":"https://doi.org/10.1042/bj2050593","pdf_url":null,"source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1158526","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1158526","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem J","raw_type":"Text"},{"id":"pmid:6293459","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6293459","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Biochemical journal","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:1158526","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1158526","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem J","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W87082738","https://openalex.org/W207591095","https://openalex.org/W1266082629","https://openalex.org/W1502564761","https://openalex.org/W1513897103","https://openalex.org/W1603383904","https://openalex.org/W1799692929","https://openalex.org/W1940436875","https://openalex.org/W1967755668","https://openalex.org/W1967833122","https://openalex.org/W1970902024","https://openalex.org/W1980722232","https://openalex.org/W1981903503","https://openalex.org/W1982119222","https://openalex.org/W1983153204","https://openalex.org/W1986979815","https://openalex.org/W1990849016","https://openalex.org/W2028472874","https://openalex.org/W2046543299","https://openalex.org/W2065212708","https://openalex.org/W2080418255","https://openalex.org/W2083322279","https://openalex.org/W2086336856","https://openalex.org/W2088897022","https://openalex.org/W2096207937","https://openalex.org/W2156060851","https://openalex.org/W2168526937","https://openalex.org/W2408109271","https://openalex.org/W2415052736","https://openalex.org/W3023773984"],"related_works":["https://openalex.org/W1773050201","https://openalex.org/W2291102027","https://openalex.org/W2086702401","https://openalex.org/W652937351","https://openalex.org/W2149186978","https://openalex.org/W2023019821","https://openalex.org/W1526061497","https://openalex.org/W2411865488","https://openalex.org/W2083843475","https://openalex.org/W1972912591"],"abstract_inverted_index":{"A":[0,118],"phagocytic":[1,56],"vesicle":[2,57,107,182],"fraction":[3,108,183],"with":[4,70,91,97,133],"high":[5],"NADPH-dependent":[6],"superoxide-forming":[7,63,82],"activity":[8,28,48,64,83,147],"was":[9,36,52,65,129,151,175],"obtained":[10],"in":[11,22,49,54,131,180],"large":[12],"quantity":[13],"from":[14],"pig":[15],"blood":[16],"polymorphonuclear":[17],"leucocytes,":[18],"phagocytosing":[19,34],"oil":[20],"droplets":[21],"the":[23,30,33,41,47,50,55,62,106,134,149,155,161,167],"presence":[24],"of":[25,29,32,40,46,61,84,89,95,105,121,148,157,166],"cyanide.":[26],"The":[27,78,146,170,178],"homogenate":[31,51],"cells":[35],"40":[37],"times":[38],"that":[39,160],"resting":[42],"cells,":[43],"and":[44,75,93,113,126,184],"70%":[45],"recovered":[53],"fraction.":[58],"Essentially":[59],"all":[60],"extracted":[66],"by":[67,154],"repeated":[68],"extraction":[69],"a":[71,81,164],"mixture":[72],"containing":[73],"deoxycholate":[74],"Tween":[76],"20.":[77],"extract":[79,150,185],"had":[80],"1":[85],"mumol/min":[86],"per":[87],"mg":[88],"protein":[90],"NADPH,":[92],"one-fifth":[94],"this":[96],"NADH,":[98],"Km":[99,171],"values":[100],"being":[101],"similar":[102],"to":[103,139],"those":[104],"(40":[109],"microM":[110,115],"for":[111,116,123,173,195],"NADPH":[112,124,136],"400":[114],"NADH).":[117],"stoichiometric":[119],"relationship":[120],"1:2":[122],"oxidation":[125],"superoxide":[127],"formation":[128],"obtained,":[130],"agreement":[132],"reaction":[135],"+2O2":[137],"leads":[138],"NADP+":[140],"+":[141,144],"2O2":[142],"-.":[143],"H+.":[145],"enhanced":[152],"2-fold":[153],"addition":[156],"FAD,":[158],"suggesting":[159],"flavin":[162],"is":[163],"component":[165],"enzyme":[168],"system.":[169],"value":[172],"FAD":[174],"0.077":[176],"microM.":[177],"activities":[179],"both":[181],"were":[186],"labile":[187],"even":[188],"on":[189],"refrigeration,":[190],"but":[191],"could":[192],"be":[193],"kept":[194],"several":[196],"months":[197],"at":[198],"-70":[199],"degrees":[200],"C.":[201]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}