{"id":"https://openalex.org/W640484614","doi":null,"title":"Application of proximity ligation for detection of proteins, biomolecular interactions, and single copies of pathogens.","display_name":"Application of proximity ligation for detection of proteins, biomolecular interactions, and single copies of pathogens.","publication_year":2006,"publication_date":"2006-01-01","ids":{"openalex":"https://openalex.org/W640484614","mag":"640484614"},"language":"en","primary_location":{"id":"pmh:oai:DiVA.org:uu-6791","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6791","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6791","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005314759","display_name":"Sigrun Gustafsdottir","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Sigrun Gustafsdottir","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5005314759"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9365000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9365000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.03530000150203705,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.01549999974668026,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/proximity-ligation","display_name":"proximity ligation","score":0.9670000076293945},{"id":"https://openalex.org/keywords/proximity-probes","display_name":"proximity probes","score":0.828000009059906},{"id":"https://openalex.org/keywords/protein-detection","display_name":"protein detection","score":0.7120000123977661},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.6669999957084656},{"id":"https://openalex.org/keywords/protein-nucleic-acid-interactions","display_name":"protein-nucleic acid interactions","score":0.878000020980835},{"id":"https://openalex.org/keywords/pathogen-detection","display_name":"pathogen detection","score":0.8320000171661377},{"id":"https://openalex.org/keywords/viral-surface-proteins","display_name":"viral surface proteins","score":0.48100000619888306},{"id":"https://openalex.org/keywords/bacterial-surface-proteins","display_name":"bacterial surface proteins","score":0.8339999914169312},{"id":"https://openalex.org/keywords/parvovirus","display_name":"parvovirus","score":0.9070000052452087},{"id":"https://openalex.org/keywords/intracellular-bacteria","display_name":"intracellular bacteria","score":0.9639999866485596},{"id":"https://openalex.org/keywords/vegf-a","display_name":"VEGF-A","score":0.8569999933242798},{"id":"https://openalex.org/keywords/vegfr1","display_name":"VEGFR1","score":0.996999979019165},{"id":"https://openalex.org/keywords/vegfr2","display_name":"VEGFR2","score":0.9980000257492065},{"id":"https://openalex.org/keywords/nucleic-acid-amplification","display_name":"nucleic acid amplification","score":0.8659999966621399}],"concepts":[{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.8249754905700684},{"id":"https://openalex.org/C2776243700","wikidata":"https://www.wikidata.org/wiki/Q7252869","display_name":"Proximity ligation assay","level":3,"score":0.6500260829925537},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5764350891113281},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4887993037700653},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.4566075801849365},{"id":"https://openalex.org/C179437574","wikidata":"https://www.wikidata.org/wiki/Q15089798","display_name":"Ligation","level":2,"score":0.4562554955482483},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4375305771827698},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.4159037470817566},{"id":"https://openalex.org/C524204448","wikidata":"https://www.wikidata.org/wiki/Q788926","display_name":"Infectious disease (medical specialty)","level":3,"score":0.41377532482147217},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34603917598724365},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.32635802030563354},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.25927305221557617},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.09473705291748047},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.09353116154670715},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.09097519516944885},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07982996106147766},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:DiVA.org:uu-6791","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6791","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},{"id":"mag:640484614","doi":null,"is_oa":false,"landing_page_url":"http://uu.diva-portal.org/smash/get/diva2:168121/FULLTEXT01.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:DiVA.org:uu-6791","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6791","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.5683000087738037}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":119,"referenced_works":["https://openalex.org/W118777696","https://openalex.org/W207250171","https://openalex.org/W1502702971","https://openalex.org/W1504247884","https://openalex.org/W1507443244","https://openalex.org/W1522655814","https://openalex.org/W1569275112","https://openalex.org/W1587020653","https://openalex.org/W1599833391","https://openalex.org/W1658092509","https://openalex.org/W1884509322","https://openalex.org/W1918337600","https://openalex.org/W1936218468","https://openalex.org/W1943682695","https://openalex.org/W1953216172","https://openalex.org/W1969328947","https://openalex.org/W1969416735","https://openalex.org/W1970302207","https://openalex.org/W1970401341","https://openalex.org/W1970620899","https://openalex.org/W1970761420","https://openalex.org/W1971282098","https://openalex.org/W1971428641","https://openalex.org/W1977186059","https://openalex.org/W1978829580","https://openalex.org/W1982401705","https://openalex.org/W1985521695","https://openalex.org/W1985900564","https://openalex.org/W1986352563","https://openalex.org/W1987416224","https://openalex.org/W1989578749","https://openalex.org/W1990557050","https://openalex.org/W1991004122","https://openalex.org/W1994091239","https://openalex.org/W1994778563","https://openalex.org/W1996514181","https://openalex.org/W1997458078","https://openalex.org/W2002903950","https://openalex.org/W2003470884","https://openalex.org/W2004563262","https://openalex.org/W2006774455","https://openalex.org/W2006813546","https://openalex.org/W2008148453","https://openalex.org/W2010649076","https://openalex.org/W2010888033","https://openalex.org/W2012536704","https://openalex.org/W2014950129","https://openalex.org/W2014993834","https://openalex.org/W2017471463","https://openalex.org/W2017926405","https://openalex.org/W2018385269","https://openalex.org/W2023096047","https://openalex.org/W2025968178","https://openalex.org/W2030189518","https://openalex.org/W2030522563","https://openalex.org/W2031762818","https://openalex.org/W2031988539","https://openalex.org/W2034545703","https://openalex.org/W2044809585","https://openalex.org/W2046507292","https://openalex.org/W2047196913","https://openalex.org/W2049852228","https://openalex.org/W2049869291","https://openalex.org/W2050149029","https://openalex.org/W2050163545","https://openalex.org/W2050717506","https://openalex.org/W2053697292","https://openalex.org/W2054434856","https://openalex.org/W2056285082","https://openalex.org/W2059939467","https://openalex.org/W2060913290","https://openalex.org/W2064680209","https://openalex.org/W2065304353","https://openalex.org/W2070360032","https://openalex.org/W2076675639","https://openalex.org/W2077224933","https://openalex.org/W2079321730","https://openalex.org/W2079522334","https://openalex.org/W2080155148","https://openalex.org/W2082836642","https://openalex.org/W2083813449","https://openalex.org/W2084151531","https://openalex.org/W2084855813","https://openalex.org/W2084938664","https://openalex.org/W2089282467","https://openalex.org/W2091857802","https://openalex.org/W2092956762","https://openalex.org/W2093223464","https://openalex.org/W2094022774","https://openalex.org/W2097303612","https://openalex.org/W2104525153","https://openalex.org/W2109250419","https://openalex.org/W2111208057","https://openalex.org/W2117755410","https://openalex.org/W2118304741","https://openalex.org/W2118628540","https://openalex.org/W2120226003","https://openalex.org/W2124395350","https://openalex.org/W2130494035","https://openalex.org/W2131025546","https://openalex.org/W2134672977","https://openalex.org/W2139377022","https://openalex.org/W2139580799","https://openalex.org/W2141155302","https://openalex.org/W2143784594","https://openalex.org/W2149748824","https://openalex.org/W2150566147","https://openalex.org/W2152068169","https://openalex.org/W2157208987","https://openalex.org/W2165011536","https://openalex.org/W2165460636","https://openalex.org/W2168148516","https://openalex.org/W2168909179","https://openalex.org/W2170949754","https://openalex.org/W2172116519","https://openalex.org/W2316323923","https://openalex.org/W2774846296","https://openalex.org/W3023173633","https://openalex.org/W3150248096"],"related_works":["https://openalex.org/W212081039","https://openalex.org/W306778785","https://openalex.org/W2017766056","https://openalex.org/W127833257","https://openalex.org/W1976658182","https://openalex.org/W167931038","https://openalex.org/W302496292","https://openalex.org/W1977093496","https://openalex.org/W2519712108","https://openalex.org/W2092956762"],"abstract_inverted_index":{"Proximity":[0,60,170],"ligation":[1,80,118,171,263],"is":[2,51],"a":[3,30,207],"recently":[4],"established":[5,243],"technique":[6],"that":[7],"can":[8,165],"provide":[9,166],"answers":[10],"to":[11,53,67,70,74,175,193,267,281],"questions":[12],"about":[13],"the":[14,34,45,54,87,98,106,110,113,116,162,261,283],"concentration,":[15],"localization,":[16],"interactions,":[17],"modifications":[18],"and":[19,73,130,133,180,209,227,233,247,255,272,278,280],"functions":[20],"of":[21,47,56,89,100,115,122,126,134,136,145,157,178,184,195,220,229,238,251,274,285],"proteins.":[22,189],"The":[23],"method":[24],"enables":[25],"sensitive":[26,248],"protein":[27],"measurements":[28],"with":[29,147,222],"detection":[31,121,144,183,198],"limit":[32],"in":[33,38,83,105,203,224],"low":[35],"femtomolar":[36],"range":[37],"complex":[39],"biological":[40],"samples.":[41],"In":[42,108],"proximity":[43,95,117,262],"ligation,":[44],"challenge":[46],"detecting":[48],"specific":[49,57,143,246,256],"proteins":[50,129,221,232,254],"converted":[52],"analysis":[55,125,156,250],"DNA":[58],"sequences.":[59,259],"probes":[61,96],"containing":[62],"oligonucleotide":[63,101],"extensions":[64],"are":[65,103,151,216,236],"designed":[66],"bind":[68],"pairwise":[69],"target":[71],"proteins,":[72],"form":[75],"amplifiable":[76],"tag":[77],"sequences":[78,102],"upon":[79],"when":[81,155],"brought":[82],"proximity.":[84],"Protocols":[85,241],"for":[86,120,206,210,244],"conversion":[88],"monoclonal":[90],"or":[91,161,186],"polyclonal":[92],"antibodies":[93],"into":[94],"through":[97],"attachment":[99],"described":[104],"thesis.":[107],"addition,":[109],"thesis":[111],"describes":[112],"adaptation":[114],"technology":[119],"microbial":[123],"pathogens,":[124],"interactions":[127,219,230,252,269],"between":[128,231,253,270],"nucleic":[131,257],"acids,":[132],"inhibition":[135],"receptor-ligand":[137],"interactions.":[138],"Nucleic":[139],"acid":[140,258],"amplification":[141],"allows":[142],"pathogens":[146],"single-copy":[148],"sensitivity.":[149],"There":[150],"many":[152],"circumstances,":[153],"however,":[154],"pathogen":[158],"surface":[159,188],"antigens":[160],"antibody":[163],"response":[164],"increased":[167],"diagnostic":[168],"value.":[169],"reactions":[172,199],"were":[173,200,242],"used":[174,266],"measure":[176],"numbers":[177],"virus":[179],"bacteria":[181],"by":[182,218],"viral":[185],"bacterial":[187],"Detection":[190],"sensitivities":[191],"similar":[192],"those":[194],"nuclear":[196],"acid-based":[197],"achieved":[201],"directly":[202],"infected":[204],"samples":[205],"parvovirus":[208],"an":[211],"intracellular":[212],"bacterium.":[213],"Biological":[214],"processes":[215],"orchestrated":[217],"molecules":[223],"their":[225],"environment,":[226],"investigations":[228],"other":[234],"biomolecules":[235],"thus":[237],"great":[239],"importance.":[240],"very":[245],"homogeneous-phase":[249],"Finally,":[260],"mechanism":[264],"was":[265],"monitor":[268],"VEGF-A":[271],"two":[273],"its":[275],"receptors,":[276],"VEGFR-1":[277],"VEGFR-2,":[279],"characterize":[282],"effects":[284],"agents":[286],"disrupting":[287],"this":[288],"interaction.":[289]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2016-06-24T00:00:00"}
