{"id":"https://openalex.org/W6375506","doi":"https://doi.org/10.1007/978-1-61779-876-4_2","title":"In-Fusion\u00ae Cloning with Vaccinia Virus DNA Polymerase","display_name":"In-Fusion\u00ae Cloning with Vaccinia Virus DNA Polymerase","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W6375506","doi":"https://doi.org/10.1007/978-1-61779-876-4_2","mag":"6375506","pmid":"https://pubmed.ncbi.nlm.nih.gov/22688759"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-61779-876-4_2","doi":"https://doi.org/10.1007/978-1-61779-876-4_2","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-61779-876-4_2","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2500395158","display_name":"Vaccinia Virus and Poxvirology"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088666106","display_name":"Chad R. Irwin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154425047","display_name":"University of Alberta","ror":"https://ror.org/0160cpw27","country_code":"CA","type":"education","lineage":["https://openalex.org/I154425047"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Chad R. Irwin","raw_affiliation_strings":["Department of Medical Microbiology and Immunology, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Microbiology and Immunology, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada","institution_ids":["https://openalex.org/I154425047"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060924502","display_name":"Andrew A. Farmer","orcid":"https://orcid.org/0000-0002-6170-4402","observed_orcids":["https://orcid.org/0000-0002-6170-4402","https://orcid.org/0000-0002-4224-2433"]},"institutions":[{"id":"https://openalex.org/I4210131176","display_name":"Takara (United States)","ror":"https://ror.org/02js6ft04","country_code":"US","type":"company","lineage":["https://openalex.org/I4210113000","https://openalex.org/I4210131176"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew Farmer","raw_affiliation_strings":["Clontech Laboratories, Inc., Mountain View, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clontech Laboratories, Inc., Mountain View, CA, USA","institution_ids":["https://openalex.org/I4210131176"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000670265","display_name":"David O. Willer","orcid":"https://orcid.org/0000-0002-9763-8988","observed_orcids":["https://orcid.org/0000-0002-9763-8988"]},"institutions":[{"id":"https://openalex.org/I1291836623","display_name":"Mount Sinai Hospital","ror":"https://ror.org/05deks119","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I1291836623","https://openalex.org/I4210130843"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"David O. Willer","raw_affiliation_strings":["Department of Microbiology, Mt. Sinai Hospital, Toronto, ON, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Mt. Sinai Hospital, Toronto, ON, Canada","institution_ids":["https://openalex.org/I1291836623"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5020926082","display_name":"David H. Evans","orcid":"https://orcid.org/0000-0001-5871-299X","observed_orcids":["https://orcid.org/0000-0001-5871-299X"]},"institutions":[{"id":"https://openalex.org/I154425047","display_name":"University of Alberta","ror":"https://ror.org/0160cpw27","country_code":"CA","type":"education","lineage":["https://openalex.org/I154425047"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"David H. Evans","raw_affiliation_strings":["Department of Medical Microbiology and Immunology, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Microbiology and Immunology, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, AB, Canada","institution_ids":["https://openalex.org/I154425047"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5020926082"],"corresponding_institution_ids":["https://openalex.org/I154425047"],"apc_list":null,"apc_paid":null,"fwci":8.6018,"has_fulltext":false,"cited_by_count":46,"citation_normalized_percentile":{"value":0.98136646,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"890","issue":null,"first_page":"23","last_page":"35"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11767","display_name":"Poxvirus research and outbreaks","score":0.8935999870300293,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11767","display_name":"Poxvirus research and outbreaks","score":0.8935999870300293,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.042500000447034836,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.03880000114440918,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/recombineering","display_name":"recombineering","score":0.8379999995231628},{"id":"https://openalex.org/keywords/directional-cloning","display_name":"directional cloning","score":0.8619999885559082},{"id":"https://openalex.org/keywords/pcr-cloning","display_name":"PCR cloning","score":0.7319999933242798},{"id":"https://openalex.org/keywords/proofreading-exonuclease","display_name":"proofreading exonuclease","score":0.8159999847412109},{"id":"https://openalex.org/keywords/single-strand-annealing","display_name":"single-strand annealing","score":0.8690000176429749},{"id":"https://openalex.org/keywords/joint-molecules","display_name":"joint molecules","score":0.7990000247955322},{"id":"https://openalex.org/keywords/single-stranded-dna-binding-protein","display_name":"single-stranded DNA binding protein","score":0.8069999814033508}],"concepts":[{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.5760317444801331},{"id":"https://openalex.org/C2777501222","wikidata":"https://www.wikidata.org/wiki/Q411664","display_name":"Exonuclease","level":4,"score":0.5733128786087036},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.5663045644760132},{"id":"https://openalex.org/C2781356689","wikidata":"https://www.wikidata.org/wiki/Q1986297","display_name":"Vaccinia","level":4,"score":0.514865517616272},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5126404166221619},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.4785609543323517},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4688739776611328},{"id":"https://openalex.org/C203284983","wikidata":"https://www.wikidata.org/wiki/Q17087215","display_name":"In vitro recombination","level":5,"score":0.42552995681762695},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.42391934990882874},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4235181212425232},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.41195547580718994},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.35051149129867554},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3366636633872986},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.29887154698371887},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2886914908885956},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.1728675365447998},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.07969272136688232},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002522","descriptor_name":"Chlorocebus aethiops","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004259","descriptor_name":"DNA-Directed DNA Polymerase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004274","descriptor_name":"DNA, Recombinant","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005814","descriptor_name":"Genes, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014616","descriptor_name":"Vaccinia virus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014776","descriptor_name":"Virus Cultivation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-61779-876-4_2","doi":"https://doi.org/10.1007/978-1-61779-876-4_2","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-61779-876-4_2","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:22688759","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22688759","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334506","display_name":"Canadian Institutes of Health Research","ror":"https://ror.org/01gavpb45"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W1559291368","https://openalex.org/W1951707121","https://openalex.org/W1997972699","https://openalex.org/W2013970428","https://openalex.org/W2016728250","https://openalex.org/W2043052104","https://openalex.org/W2052107881","https://openalex.org/W2063723654","https://openalex.org/W2063730556","https://openalex.org/W2066298830","https://openalex.org/W2100856120","https://openalex.org/W2103747393","https://openalex.org/W2123786479","https://openalex.org/W2132788601","https://openalex.org/W2133034192","https://openalex.org/W2144564648","https://openalex.org/W2160873483","https://openalex.org/W2322672979"],"related_works":["https://openalex.org/W2013970428","https://openalex.org/W2063730556","https://openalex.org/W3163801064","https://openalex.org/W2123786479","https://openalex.org/W7099634244","https://openalex.org/W2144564648","https://openalex.org/W30659429","https://openalex.org/W2133034192","https://openalex.org/W2011152880","https://openalex.org/W2042693796"],"abstract_inverted_index":{"Vaccinia":[0,158],"virus":[1,31,159],"DNA":[2,17,21,161],"polymerase":[3],"(VVpol)":[4],"encodes":[5],"a":[6,26,64,135],"3'-to-5'":[7],"proofreading":[8],"exonuclease":[9],"that":[10,55,88,114,127],"can":[11,39,58,164],"degrade":[12],"the":[13,68,103,124,131,150,208,211,216],"ends":[14,133,152],"of":[15,71,77,82,93,118,123,134,210],"duplex":[16],"and":[18,141,153],"expose":[19],"single-stranded":[20,160],"tails.":[22],"The":[23,138,178],"reaction":[24,57],"plays":[25],"critical":[27],"role":[28],"in":[29,33,61,104],"promoting":[30],"recombination":[32],"vivo":[34],"because":[35],"single-strand":[36],"annealing":[37],"reactions":[38],"then":[40],"fuse":[41],"molecules":[42,181,193],"sharing":[43],"complementary":[44,151],"tails":[45],"into":[46,74,194,215],"recombinant":[47],"precursors":[48],"called":[49,86],"joint":[50,155,180],"molecules.":[51],"We":[52,197],"have":[53],"shown":[54],"this":[56,83,169,200],"also":[59],"occur":[60],"vitro,":[62],"providing":[63],"simple":[65],"method":[66],"for":[67],"directional":[69,91],"cloning":[70,92,106,209],"PCR":[72,94,108,125,142],"products":[73,95,109,143],"any":[75],"vector":[76,140,217],"interest.":[78],"A":[79],"commercial":[80],"form":[81],"recombineering":[84],"technology":[85,201],"In-Fusion(\u00ae)":[87],"facilitates":[89],"high-throughput":[90],"has":[96],"been":[97],"commercialized":[98],"by":[99,203],"Clontech.":[100],"To":[101],"effect":[102],"vitro":[105],"reaction,":[107,170],"are":[110,128,144,182],"prepared":[111],"using":[112],"primers":[113],"add":[115],"16-18":[116],"bp":[117],"sequence":[119],"to":[120,130,167,184],"each":[121],"end":[122],"amplicon":[126],"homologous":[129],"two":[132],"linearized":[136,139],"vector.":[137],"coincubated":[145],"with":[146],"VVpol,":[147],"which":[148,188],"exposes":[149],"promotes":[154],"molecule":[156],"formation.":[157],"binding":[162],"protein":[163],"be":[165],"added":[166],"enhance":[168],"although":[171],"it":[172],"is":[173],"not":[174],"an":[175,206],"essential":[176],"component.":[177],"resulting":[179],"used":[183],"transform":[185],"E.":[186],"coli,":[187],"convert":[189],"these":[190],"noncovalently":[191],"joined":[192],"stable":[195],"recombinants.":[196],"illustrate":[198],"how":[199],"works":[202],"using,":[204],"as":[205],"example,":[207],"vaccinia":[212],"N2L":[213],"gene":[214],"pETBlue-2.":[218]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}