{"id":"https://openalex.org/W625659430","doi":null,"title":"Identification of Novel Regulators in Hematopoiesis: Roles for Gfer in Hematopoietic Stem Cell Proliferation and CaMKK2 in the Restriction of Granulopoiesis","display_name":"Identification of Novel Regulators in Hematopoiesis: Roles for Gfer in Hematopoietic Stem Cell Proliferation and CaMKK2 in the Restriction of Granulopoiesis","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W625659430","mag":"625659430"},"language":"en","primary_location":{"id":"pmh:oai:dukespace.lib.duke.edu:10161/5713","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10161/5713","pdf_url":null,"source":{"id":"https://openalex.org/S4306400687","display_name":"DukeSpace (Duke University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I170897317","host_organization_name":"Duke University","host_organization_lineage":["https://openalex.org/I170897317"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061420340","display_name":"Ellen C. Teng","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ellen C. Teng","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5061420340"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12919","display_name":"Blood disorders and treatments","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hematopoietic-stem-cells","display_name":"hematopoietic stem cells","score":0.9240000247955322},{"id":"https://openalex.org/keywords/camkiv","display_name":"CaMKIV","score":0.7379999756813049},{"id":"https://openalex.org/keywords/camkk2","display_name":"CAMKK2","score":0.8420000076293945},{"id":"https://openalex.org/keywords/granulopoiesis","display_name":"granulopoiesis","score":0.890999972820282},{"id":"https://openalex.org/keywords/p27","display_name":"p27","score":0.8840000033378601},{"id":"https://openalex.org/keywords/jab1","display_name":"Jab1","score":0.9430000185966492},{"id":"https://openalex.org/keywords/cop9-signalosome","display_name":"COP9 signalosome","score":0.9259999990463257},{"id":"https://openalex.org/keywords/ubiquitination","display_name":"ubiquitination","score":0.9139999747276306},{"id":"https://openalex.org/keywords/hsc-self-renewal","display_name":"HSC self-renewal","score":0.6840000152587891},{"id":"https://openalex.org/keywords/hsc-exhaustion","display_name":"HSC exhaustion","score":0.6610000133514404},{"id":"https://openalex.org/keywords/hsc-proliferation","display_name":"HSC proliferation","score":0.41600000858306885},{"id":"https://openalex.org/keywords/calcium-calmodulin-signaling","display_name":"calcium/calmodulin signaling","score":0.6299999952316284},{"id":"https://openalex.org/keywords/hematopoiesis-regulation","display_name":"hematopoietic regulation","score":0.5339999794960022},{"id":"https://openalex.org/keywords/rnai-screen","display_name":"RNAi screen","score":0.4790000021457672}],"concepts":[{"id":"https://openalex.org/C2779452902","wikidata":"https://www.wikidata.org/wiki/Q5596845","display_name":"Granulopoiesis","level":4,"score":0.9157187938690186},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.7697938680648804},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.6354354023933411},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5381819009780884},{"id":"https://openalex.org/C2778086970","wikidata":"https://www.wikidata.org/wiki/Q514525","display_name":"Hematopoietic stem cell","level":4,"score":0.475763738155365},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.475715696811676},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.4103999435901642},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3344535827636719},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:dukespace.lib.duke.edu:10161/5713","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10161/5713","pdf_url":null,"source":{"id":"https://openalex.org/S4306400687","display_name":"DukeSpace (Duke University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I170897317","host_organization_name":"Duke University","host_organization_lineage":["https://openalex.org/I170897317"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dissertation"},{"id":"mag:625659430","doi":null,"is_oa":false,"landing_page_url":"https://dukespace.lib.duke.edu/dspace/bitstream/10161/5713/1/Teng_duke_0066D_11089.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":131,"referenced_works":["https://openalex.org/W215245711","https://openalex.org/W1484384039","https://openalex.org/W1487054855","https://openalex.org/W1496297920","https://openalex.org/W1500240510","https://openalex.org/W1508593718","https://openalex.org/W1517420663","https://openalex.org/W1544012135","https://openalex.org/W1549203021","https://openalex.org/W1569324890","https://openalex.org/W1583066631","https://openalex.org/W1588399796","https://openalex.org/W1669954301","https://openalex.org/W1782908816","https://openalex.org/W1875254393","https://openalex.org/W1930917734","https://openalex.org/W1952592959","https://openalex.org/W1966382091","https://openalex.org/W1969477930","https://openalex.org/W1970205234","https://openalex.org/W1970634781","https://openalex.org/W1973035870","https://openalex.org/W1975886438","https://openalex.org/W1982085282","https://openalex.org/W1983925806","https://openalex.org/W1987598864","https://openalex.org/W1991962444","https://openalex.org/W1995286997","https://openalex.org/W1998566378","https://openalex.org/W2001627351","https://openalex.org/W2003371248","https://openalex.org/W2004491895","https://openalex.org/W2005727437","https://openalex.org/W2008680658","https://openalex.org/W2009728195","https://openalex.org/W2009758262","https://openalex.org/W2010238890","https://openalex.org/W2011174877","https://openalex.org/W2011458540","https://openalex.org/W2012968622","https://openalex.org/W2015632708","https://openalex.org/W2016810616","https://openalex.org/W2017456229","https://openalex.org/W2017583361","https://openalex.org/W2018716983","https://openalex.org/W2021440803","https://openalex.org/W2026417885","https://openalex.org/W2027309262","https://openalex.org/W2027397115","https://openalex.org/W2029189134","https://openalex.org/W2031338420","https://openalex.org/W2035502959","https://openalex.org/W2035559275","https://openalex.org/W2035635176","https://openalex.org/W2038405912","https://openalex.org/W2039748263","https://openalex.org/W2040213449","https://openalex.org/W2040927716","https://openalex.org/W2041926656","https://openalex.org/W2046274893","https://openalex.org/W2046839020","https://openalex.org/W2048201722","https://openalex.org/W2054595891","https://openalex.org/W2057347947","https://openalex.org/W2057396381","https://openalex.org/W2058483875","https://openalex.org/W2061341693","https://openalex.org/W2061412811","https://openalex.org/W2062162181","https://openalex.org/W2062353253","https://openalex.org/W2063185070","https://openalex.org/W2063842496","https://openalex.org/W2064641679","https://openalex.org/W2068412840","https://openalex.org/W2070154483","https://openalex.org/W2070362241","https://openalex.org/W2074112908","https://openalex.org/W2076493773","https://openalex.org/W2077330278","https://openalex.org/W2079352097","https://openalex.org/W2081654176","https://openalex.org/W2083456111","https://openalex.org/W2083483484","https://openalex.org/W2083632015","https://openalex.org/W2084203912","https://openalex.org/W2086830518","https://openalex.org/W2088238130","https://openalex.org/W2089639332","https://openalex.org/W2091077356","https://openalex.org/W2091994574","https://openalex.org/W2095454212","https://openalex.org/W2095535075","https://openalex.org/W2098947405","https://openalex.org/W2099093659","https://openalex.org/W2100378064","https://openalex.org/W2102891343","https://openalex.org/W2103546044","https://openalex.org/W2108244937","https://openalex.org/W2110103733","https://openalex.org/W2110905385","https://openalex.org/W2114341896","https://openalex.org/W2118139721","https://openalex.org/W2118444707","https://openalex.org/W2119062616","https://openalex.org/W2119711962","https://openalex.org/W2120839885","https://openalex.org/W2121864465","https://openalex.org/W2123194197","https://openalex.org/W2123480703","https://openalex.org/W2124647664","https://openalex.org/W2125118099","https://openalex.org/W2126995384","https://openalex.org/W2127621383","https://openalex.org/W2132362370","https://openalex.org/W2132757051","https://openalex.org/W2132954098","https://openalex.org/W2134237864","https://openalex.org/W2135135539","https://openalex.org/W2137932281","https://openalex.org/W2139516400","https://openalex.org/W2140925666","https://openalex.org/W2151024354","https://openalex.org/W2154776006","https://openalex.org/W2160855812","https://openalex.org/W2165625463","https://openalex.org/W2170411747","https://openalex.org/W2171070506","https://openalex.org/W2301127604","https://openalex.org/W2399252479","https://openalex.org/W2417180220","https://openalex.org/W4245703403"],"related_works":["https://openalex.org/W2017456229","https://openalex.org/W1975886438","https://openalex.org/W2070847551","https://openalex.org/W2588831272","https://openalex.org/W2551381301","https://openalex.org/W2012886518","https://openalex.org/W7163507997","https://openalex.org/W2981023329","https://openalex.org/W7160069660","https://openalex.org/W2127851311"],"abstract_inverted_index":{"Hematopoiesis":[0],"is":[1,17,24,44,88],"the":[2,63,104,117,149,235,276,321,385,392,396,406,434,441,475,565,573,587,603],"process":[3,23],"in":[4,97,112,131,173,198,202,247,250,300,312,328,333,347,374,395,405,433,440,474,482,497,507,530,551,564,568,572,583],"which":[5],"billions":[6],"of":[7,41,45,50,106,140,151,160,190,196,234,245,266,297,303,309,320,326,359,366,387,408,414,437,463,479,524,589,609],"blood":[8],"cells":[9,229,431,499],"are":[10,72,597],"produced":[11],"on":[12,148,178,384],"a":[13,28,81,165,187,208,342,367,401,508,516,543,580],"daily":[14],"basis,":[15],"and":[16,37,39,70,95,134,185,206,225,254,293,318,371,376,412,424,444,468,494,528,541,546,585,594,602,605],"vital":[18],"for":[19,91,119,341,391,403,519,599],"sustaining":[20],"life.":[21],"This":[22],"tightly":[25],"regulated":[26],"by":[27,221,428],"dynamic":[29],"balance":[30,43],"between":[31],"hematopoietic":[32,55,113,377],"stem":[33],"cell":[34,452],"(HSC)":[35],"self-renewal":[36],"differentiation,":[38,493],"maintenance":[40],"this":[42,141,360],"critical":[46],"importance":[47,105],"as":[48,60,125,521,570],"dysregulation":[49],"HSCs":[51,133,159,176,199,220,248,330,348,375,569],"can":[52],"lead":[53,289],"to":[54,128,156,170,213,262,290,305,316,351,455,489,503,539,549],"deficiencies":[56],"or":[57,561],"malignancies":[58],"such":[59,124],"leukemogenesis.":[61],"While":[62,534],"signaling":[64,111],"mechanisms":[65],"that":[66,87,194,211,227,286,295,349,426,457,559,596],"regulate":[67],"HSC":[68,600],"homeostasis":[69],"function":[71,563],"not":[73,102,557],"well":[74],"understood,":[75],"our":[76,364,513,535,553,577],"previous":[77],"studies":[78,365,380,423],"have":[79],"identified":[80,400],"calcium/calmodulin":[82],"(CaM)-dependent":[83],"protein":[84,283],"kinase,":[85,369],"CaMKIV,":[86],"intrinsically":[89],"required":[90],"regulating":[92,132],"normal":[93],"proliferation":[94,335,601],"survival":[96],"HSCs.":[98],"These":[99,337,379],"findings":[100,578],"suggest":[101,558],"only":[103],"calcium-initiated":[107],"pathways":[108],"including":[109],"CaMKIV-dependent":[110],"cells,":[114,467],"but":[115],"also":[116,501],"potential":[118,188,205],"other":[120,126],"calcium/CaM-dependent":[121],"effector":[122],"proteins":[123],"CaM-kinases":[127],"be":[129,171],"involved":[130],"hematopoiesis.":[135],"The":[136,355],"first":[137],"major":[138,357],"section":[139,358],"dissertation":[142,361],"work":[143,362],"presented":[144],"herein":[145],"was":[146,487,500,538],"based":[147,177,383],"usage":[150,386],"RNA":[152],"interference":[153],"(RNAi)":[154],"technology":[155],"specifically":[157],"deplete":[158],"growth":[161],"factor":[162],"erv1-like":[163],"(Gfer),":[164],"gene":[166,394],"whose":[167],"expression":[168],"appeared":[169],"absent":[172],"CaMKIV":[174,368,550],"null":[175],"comparative":[179],"microarray":[180],"analysis":[181],"with":[182,273],"wild-type":[183],"HSCs,":[184,552],"seemed":[186],"target":[189,545],"CaMKIV.":[191],"We":[192,216,257,418],"showed":[193],"depletion":[195,296],"Gfer":[197,246,267,298,310,560,593],"compromised":[200],"their":[201,214,332],"vivo":[203],"engraftment":[204,427],"triggered":[207],"hyper-proliferative":[209],"response":[210],"led":[212],"exhaustion.":[215],"further":[217],"assessed":[218],"Gfer-depleted":[219],"using":[222],"microscopy":[223],"techniques":[224],"found":[226],"these":[228],"possessed":[230,460],"significantly":[231,251],"reduced":[232],"levels":[233,268],"cyclin-dependent":[236],"kinase":[237,509,548],"inhibitor":[238,523],"(CDKI)":[239],"p27kip1.":[240,256],"In":[241],"contrast,":[242],"ectopic":[243,480],"over-expression":[244,308],"resulted":[249,299,311,432],"elevated":[252,461],"total":[253],"nuclear":[255],"next":[258],"performed":[259,419],"immunoprecipitation-immunoblot":[260],"analyses":[261],"determine":[263,456],"whether":[264],"alteration":[265],"would":[269,287],"affect":[270],"p27kip1's":[271],"binding":[272,282,302,315],"its":[274,291,313,372],"inhibitor,":[275],"COP9":[277],"signalosome":[278],"subunit":[279],"jun":[280],"activation-domain":[281],"1":[284],"(Jab1),":[285],"subsequently":[288],"ubiquitination,":[292],"determined":[294],"enhanced":[301,314],"p27kip1":[304,327],"Jab1.":[306],"Conversely,":[307],"Jab1":[317],"inhibition":[319],"Jab1-p27kip1":[322],"interaction.":[323],"Furthermore,":[324],"normalization":[325],"Gfer-KD":[329],"rescued":[331],"vitro":[334],"deficits.":[336],"results":[338,514,554],"provide":[339],"evidence":[340],"novel":[343,517,591],"Gfer-Jab1-p27kip1":[344],"pathway":[345,567],"present":[346],"functions":[350,588],"restrict":[352],"abnormal":[353],"proliferation.":[354],"second":[356],"describes":[363],"CaMKK2,":[370,595],"role":[373,402,518],"development.":[378],"were":[381],"largely":[382],"mice":[388,459],"genetically":[389],"ablated":[390],"Camkk2":[393],"germline.":[397],"Herein,":[398],"we":[399,448],"CaMKK2":[404,481,520,562],"restriction":[407],"granulocytic":[409,525],"fate":[410,526],"commitment":[411,527,604],"differentiation":[413,506,529,607],"myeloid":[415,465,485,532],"progenitor":[416,466],"cells.":[417],"bone":[420,442,476],"marrow":[421,443],"transplantation":[422],"discovered":[425],"Camkk2-/-":[429,458,483],"donor":[430],"increased":[435],"production":[436],"mature":[438],"granulocytes":[439],"peripheral":[445],"blood.":[446],"Similarly,":[447],"used":[449],"fluorescence":[450],"activated":[451],"sorting":[453],"(FACS)":[454],"numbers":[462],"common":[464,484],"exhibited":[469],"an":[470,522],"accelerated":[471],"granulopoietic":[472],"phenotype":[473],"marrow.":[477],"Expression":[478],"progenitors":[486],"sufficient":[488,502],"rescue":[490],"aberrant":[491],"granulocyte":[492,505],"when":[495],"over-expressed":[496],"32Dcl3":[498],"impede":[504],"activity-dependent":[510],"manner.":[511],"Collectively,":[512],"reveal":[515],"early":[531,606],"progenitors.":[533],"original":[536],"intent":[537],"identify":[540],"link":[542],"downstream":[544],"upstream":[547],"ultimately":[555],"did":[556],"same":[566],"discussed":[571],"following":[574],"chapters.":[575],"Nonetheless,":[576],"represent":[579],"considerable":[581],"advance":[582],"identifying":[584],"characterizing":[586],"two":[590],"regulators,":[592],"important":[598],"steps":[608],"granulopoiesis,":[610],"respectively.":[611]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}