{"id":"https://openalex.org/W6246819","doi":null,"title":"Applications of retrovirus-mediated expression cloning.","display_name":"Applications of retrovirus-mediated expression cloning.","publication_year":1996,"publication_date":"1996-02-01","ids":{"openalex":"https://openalex.org/W6246819","mag":"6246819","pmid":"https://pubmed.ncbi.nlm.nih.gov/8641361"},"language":"en","primary_location":{"id":"pmid:8641361","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8641361","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental hematology","raw_type":"Comparative Study"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108295290","display_name":"Misa Onishi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Onishi","raw_affiliation_strings":["DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109113819","display_name":"Shigemi M. Kinoshita","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Kinoshita","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046621656","display_name":"Yoshihiro Morikawa","orcid":"https://orcid.org/0009-0003-3221-6914","observed_orcids":["https://orcid.org/0009-0003-3221-6914"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Y Morikawa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"A Shibuya","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Shibuya","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109884689","display_name":"Joseph H. Phillips","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J Phillips","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000763253","display_name":"Lewis L. Lanier","orcid":"https://orcid.org/0000-0003-1308-3952","observed_orcids":["https://orcid.org/0000-0003-1308-3952"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L L Lanier","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112547010","display_name":"D M Gorman","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D M Gorman","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033995613","display_name":"Garry P. Nolan","orcid":"https://orcid.org/0000-0002-8862-9043","observed_orcids":["https://orcid.org/0000-0002-8862-9043"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G P Nolan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069121285","display_name":"Atsushi Miyajima","orcid":"https://orcid.org/0000-0003-3176-5906","observed_orcids":["https://orcid.org/0000-0003-3176-5906"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Miyajima","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5054497666","display_name":"Toshio Kitamura","orcid":"https://orcid.org/0000-0001-7855-1767","observed_orcids":["https://orcid.org/0000-0001-7855-1767"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Kitamura","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":8.9858,"has_fulltext":false,"cited_by_count":332,"citation_normalized_percentile":{"value":0.98458628,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"24","issue":"2","first_page":"324","last_page":"9"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/retroviral-vectors","display_name":"retroviral vectors","score":0.9710000157356262},{"id":"https://openalex.org/keywords/cdna-library","display_name":"cDNA library","score":0.753000020980835},{"id":"https://openalex.org/keywords/transduction-efficiency","display_name":"transduction efficiency","score":0.550000011920929},{"id":"https://openalex.org/keywords/hematopoietic-cell-lines","display_name":"hematopoietic cell lines","score":0.8619999885559082},{"id":"https://openalex.org/keywords/nih-3t3-cells","display_name":"NIH3T3 cells","score":0.8489999771118164},{"id":"https://openalex.org/keywords/functional-selection","display_name":"functional selection","score":0.5490000247955322},{"id":"https://openalex.org/keywords/functional-mutations","display_name":"functional mutations","score":0.4480000138282776}],"concepts":[{"id":"https://openalex.org/C2778431562","wikidata":"https://www.wikidata.org/wiki/Q189308","display_name":"Retrovirus","level":3,"score":0.8810408115386963},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.7674382328987122},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.7529624700546265},{"id":"https://openalex.org/C2777187973","wikidata":"https://www.wikidata.org/wiki/Q5421703","display_name":"Expression cloning","level":4,"score":0.7072263956069946},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.6476043462753296},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.63114994764328},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.5199317336082458},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.48219457268714905},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.4787032902240753},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4377537965774536},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.4248456358909607},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.4137776494026184},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.34633293747901917},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.26406991481781006},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2352096140384674},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.10261410474777222},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.08413368463516235},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000954","descriptor_name":"Antigens, Surface","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D000954","descriptor_name":"Antigens, Surface","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004252","descriptor_name":"DNA Mutational Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004274","descriptor_name":"DNA, Recombinant","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010714","descriptor_name":"Phosphatidylethanolamines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012190","descriptor_name":"Retroviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015967","descriptor_name":"Gene Expression Regulation, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016475","descriptor_name":"3T3 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016662","descriptor_name":"Virus Integration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018076","descriptor_name":"DNA, Complementary","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:8641361","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8641361","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental hematology","raw_type":"Comparative Study"},{"id":"mag:6246819","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8641361","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.428600013256073,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G5785214559","display_name":"INTRACELLULAR T CELL REGULATORS OF HIV-1","funder_award_id":"R01AI035304","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W17552899","https://openalex.org/W7096884026","https://openalex.org/W1971244996","https://openalex.org/W1493944826","https://openalex.org/W2133835604","https://openalex.org/W1983112635","https://openalex.org/W4388386324","https://openalex.org/W1967613983","https://openalex.org/W2005341748","https://openalex.org/W2125688006"],"abstract_inverted_index":{"We":[0],"have":[1],"recently":[2],"established":[3],"a":[4,17,24,114,130],"novel":[5],"expression":[6,84,106],"cloning":[7,85,107],"system":[8,13,148],"using":[9],"retroviral":[10,26],"vectors.":[11],"The":[12,46,60],"is":[14,90,149],"based":[15],"on":[16],"high-efficiency":[18],"packaging":[19],"cell":[20,77,99,118],"line,":[21],"BOSC23,":[22],"and":[23,70],"simplified":[25],"vector,":[27,39],"pBabeX,":[28],"carrying":[29],"no":[30],"selection":[31],"marker.":[32],"cDNA":[33,58,94,131],"libraries,":[34],"constructed":[35],"in":[36,67,74,87,152,162],"the":[37,82],"pBabeX":[38],"are":[40],"transiently":[41,96],"transfected":[42],"into":[43,97,113],"BOSC23":[44],"cells.":[45],"supernatant":[47],"contains":[48],"more":[49],"than":[50],"3X10(6)/mL,":[51],"which":[52,88],"would":[53],"cover":[54],"large":[55],"complexities":[56],"of":[57,117,132,156,164],"libraries.":[59],"retrovirus":[61],"stock":[62],"gave":[63],"100%":[64],"infection":[65,72],"efficiency":[66,73],"NIH3T3":[68],"cells":[69,128],"5-40%":[71],"various":[75,135,157],"hematopoietic":[76],"lines.":[78],"In":[79],"contrast":[80],"to":[81,92,110,126],"conventional":[83],"system,":[86],"it":[89,124],"necessary":[91],"transfect":[93],"libraries":[95],"particular":[98],"types":[100],"such":[101],"as":[102],"COS":[103],"cells,":[104],"retrovirus-mediated":[105],"allows":[108],"us":[109],"transduce":[111],"cDNAs":[112],"wide":[115],"variety":[116],"types.":[119],"This":[120],"method":[121],"therefore":[122],"makes":[123],"possible":[125],"select":[127],"expressing":[129],"interest":[133],"by":[134],"functional":[136],"assays.":[137],"When":[138],"combined":[139],"with":[140],"polymerase":[141],"chain":[142],"reaction":[143],"(PCR)-driven":[144],"random":[145],"mutagenesis,":[146],"this":[147],"also":[150],"useful":[151],"searching":[153],"for":[154],"mutations":[155],"molecules":[158],"that":[159],"will":[160],"result":[161],"alterations":[163],"their":[165],"functions.":[166]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":5},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":11},{"year":2012,"cited_by_count":13}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2016-06-24T00:00:00"}