{"id":"https://openalex.org/W62020240","doi":null,"title":"Immunolocalization of the retinoblastoma protein in the human eye and in retinoblastoma.","display_name":"Immunolocalization of the retinoblastoma protein in the human eye and in retinoblastoma.","publication_year":1994,"publication_date":"1994-05-01","ids":{"openalex":"https://openalex.org/W62020240","mag":"62020240","pmid":"https://pubmed.ncbi.nlm.nih.gov/8188462"},"language":"en","primary_location":{"id":"pmid:8188462","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8188462","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5072222944","display_name":"T. Michael Nork","orcid":"https://orcid.org/0000-0002-0687-373X","observed_orcids":["https://orcid.org/0000-0002-0687-373X"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T M Nork","raw_affiliation_strings":["Department of Ophthalmology, University of Wisconsin Medical School, Madison 53792-3220"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ophthalmology, University of Wisconsin Medical School, Madison 53792-3220","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113686367","display_name":"Lyndell L. Millecchia","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L L Millecchia","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5059608917","display_name":"Gretchen L. Poulsen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G Poulsen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.2231,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":{"value":0.76460708,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"35","issue":"6","first_page":"2682","last_page":"92"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11328","display_name":"Ocular Oncology and Treatments","score":0.9717000126838684,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11328","display_name":"Ocular Oncology and Treatments","score":0.9717000126838684,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.024000000208616257,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12989","display_name":"Ocular Disorders and Treatments","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/retinoblastoma-protein","display_name":"retinoblastoma protein","score":0.9729999899864197},{"id":"https://openalex.org/keywords/retinoblastoma","display_name":"retinoblastoma","score":0.9290000200271606},{"id":"https://openalex.org/keywords/immunolocalization","display_name":"immunolocalization","score":0.8659999966621399},{"id":"https://openalex.org/keywords/antibody-specificity","display_name":"antibody specificity","score":0.5720000267028809},{"id":"https://openalex.org/keywords/formalin-fixed-paraffin-embedded-tissue","display_name":"formalin-fixed paraffin-embedded tissue","score":0.9129999876022339},{"id":"https://openalex.org/keywords/corneal-epithelium","display_name":"corneal epithelium","score":0.7979999780654907},{"id":"https://openalex.org/keywords/lens-epithelium","display_name":"lens epithelium","score":0.9779999852180481},{"id":"https://openalex.org/keywords/cell-cycle","display_name":"cell cycle","score":0.7139999866485596},{"id":"https://openalex.org/keywords/nuclear-binding","display_name":"nuclear binding","score":0.5230000019073486},{"id":"https://openalex.org/keywords/tumor-suppressor-genes","display_name":"tumor suppressor","score":0.531000018119812},{"id":"https://openalex.org/keywords/human-eye","display_name":"human eye","score":0.4350000023841858}],"concepts":[{"id":"https://openalex.org/C2776577112","wikidata":"https://www.wikidata.org/wiki/Q500695","display_name":"Retinoblastoma","level":3,"score":0.8253510594367981},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.7137514352798462},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.645591139793396},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.6455374956130981},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.5857892632484436},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5588790774345398},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.4899829924106598},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.485241174697876},{"id":"https://openalex.org/C188261085","wikidata":"https://www.wikidata.org/wiki/Q5380862","display_name":"Enucleation","level":2,"score":0.45886608958244324},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.40700283646583557},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.15295308828353882},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1494901180267334},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.11995288729667664},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.06847718358039856},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0546930730342865}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002675","descriptor_name":"Child, Preschool","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005123","descriptor_name":"Eye","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005134","descriptor_name":"Eye Neoplasms","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007124","descriptor_name":"Immunoenzyme Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008253","descriptor_name":"Macaca mulatta","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012175","descriptor_name":"Retinoblastoma","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016160","descriptor_name":"Retinoblastoma Protein","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D016161","descriptor_name":"Genes, Retinoblastoma","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmid:8188462","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8188462","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.316.5020","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.316.5020","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.iovs.org/content/35/6/2682.full.pdf","raw_type":"text"},{"id":"mag:62020240","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8188462","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3201896520","display_name":"CELLULAR MECHANISMS OF ACQUIRED COLOR VISION LOSS","funder_award_id":"R01EY008724","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"}],"funders":[{"id":"https://openalex.org/F4320337350","display_name":"National Eye Institute","ror":"https://ror.org/03wkg3b53"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W16019424","https://openalex.org/W30802970","https://openalex.org/W1533970620","https://openalex.org/W1542993965","https://openalex.org/W1754218853","https://openalex.org/W1970057173","https://openalex.org/W1975652386","https://openalex.org/W1977442544","https://openalex.org/W1988433137","https://openalex.org/W1994167061","https://openalex.org/W2001493299","https://openalex.org/W2004535553","https://openalex.org/W2012034467","https://openalex.org/W2014418134","https://openalex.org/W2024387238","https://openalex.org/W2034440981","https://openalex.org/W2038500217","https://openalex.org/W2040834490","https://openalex.org/W2052086914","https://openalex.org/W2066980099","https://openalex.org/W2077858999","https://openalex.org/W2083181195","https://openalex.org/W2099742439","https://openalex.org/W2114738441","https://openalex.org/W2115664188","https://openalex.org/W2117855786","https://openalex.org/W2155009866","https://openalex.org/W2293762542","https://openalex.org/W2396011600","https://openalex.org/W2418142884","https://openalex.org/W2436471163"],"related_works":["https://openalex.org/W7096631443","https://openalex.org/W2036002002","https://openalex.org/W2413695242","https://openalex.org/W7097385196","https://openalex.org/W1992773474","https://openalex.org/W3036068938","https://openalex.org/W2126987893","https://openalex.org/W2011023685","https://openalex.org/W2018659693","https://openalex.org/W2130624857"],"abstract_inverted_index":{"PURPOSE:":[0],"To":[1],"determine":[2,41],"whether,":[3],"by":[4,169,271],"employing":[5],"recent":[6],"advances":[7],"in":[8,26,46,52,144,159,164,189,194,232,243,252],"immunocytochemical":[9,226],"technique,":[10],"it":[11,287],"is":[12,278],"possible":[13],"to":[14,40,62,134,281],"identify":[15],"reliably":[16],"the":[17,20,37,42,111,120,129,138,145,149,174,190,195,202,253,272,289],"product":[18,275],"of":[19,44,54,110,137,148,201,265,291],"retinoblastoma":[21,244],"(RB)":[22],"susceptibility":[23],"gene,":[24],"p110RB1,":[25],"formalin-fixed,":[27],"paraffin-embedded":[28,233],"eyes":[29,49,140],"with":[30,100,211,235],"commercially":[31,236],"available":[32,237],"primary":[33],"antibodies.":[34,238],"If":[35],"so,":[36],"authors":[38],"sought":[39],"distribution":[43],"p110RB1":[45,63,221,228],"normal":[47,67,139,293],"human":[48,68,77,80],"and":[50,69,84,123,141,151,181,184,207,256],"retinoblastomas":[51],"hopes":[53],"better":[55],"understanding":[56],"its":[57],"function.":[58],"METHODS:":[59],"Four":[60],"antibodies":[61,113],"were":[64,216],"tested":[65],"on":[66,75],"monkey":[70,95],"eyes,":[71],"as":[72,74,167],"well":[73],"six":[76,175],"retinoblastomas.":[78],"The":[79,94,239],"tissue":[81],"was":[82,88,132,142,157],"formalin-fixed":[83],"paraffin-embedded.":[85],"Free":[86],"antigen":[87],"used":[89],"for":[90,171,219],"an":[91,212],"absorbed":[92],"control.":[93],"eye":[96],"had":[97,114,178,204],"been":[98],"injected":[99],"tritiated":[101],"(H3)":[102],"thymidine":[103],"24":[104],"hours":[105],"before":[106],"enucleation.":[107],"RESULTS:":[108],"Three":[109],"four":[112],"acceptable":[115],"reactivity":[116,156],"(a":[117],"polyclonal":[118],"against":[119,126],"carboxyl-terminal":[121,220],"epitope":[122],"two":[124],"monoclonals":[125],"epitopes":[127],"near":[128],"amino-terminus).":[130],"Staining":[131],"confined":[133],"nucleated":[135],"cells":[136,147,163,192,255],"strongest":[143],"cycling":[146],"lenticular":[150],"corneal":[152,161],"epithelia.":[153],"Somewhat":[154],"weaker":[155,187],"seen":[158],"those":[160],"epithelial":[162],"S":[165],"phase":[166],"determined":[168],"autoradiography":[170],"H3-thymidine.":[172],"Of":[173],"retinoblastomas,":[176],"three":[177],"strong":[179],"nuclear":[180,209,266,269],"cytoplasmic":[182,206],"staining":[183,188,210,241],"one":[185],"showed":[186],"tumor":[191,254,283],"than":[193],"adjacent":[196],"vascular":[197],"endothelial":[198],"cells.":[199],"Two":[200],"tumors":[203],"positive":[205],"negative":[208,218],"amino-terminal":[213],"antibody":[214],"but":[215,261],"completely":[217],"reactivity.":[222],"CONCLUSIONS:":[223],"Using":[224],"appropriate":[225],"techniques,":[227],"can":[229],"be":[230],"identified":[231],"tissues":[234],"observed":[240],"pattern":[242],"suggests":[245],"that":[246,257],"RB1":[247,273],"transcripts":[248],"are":[249,259],"commonly":[250],"produced":[251],"they":[258],"sometimes,":[260],"not":[262,279],"always,":[263],"capable":[264],"binding.":[267],"Thus,":[268],"binding":[270],"gene":[274],"per":[276],"se":[277],"sufficient":[280],"prevent":[282],"growth,":[284],"nor":[285],"does":[286],"indicate":[288],"presence":[290],"a":[292],"transcript.":[294]},"counts_by_year":[{"year":2018,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}