{"id":"https://openalex.org/W61598353","doi":null,"title":"Cellular Membranes as a Playground for Semliki Forest Virus Replication Complex","display_name":"Cellular Membranes as a Playground for Semliki Forest Virus Replication Complex","publication_year":2010,"publication_date":"2010-09-24","ids":{"openalex":"https://openalex.org/W61598353","mag":"61598353"},"language":"en","primary_location":{"id":"pmh:oai:helda.helsinki.fi:10138/21914","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10138/21914","pdf_url":"http://hdl.handle.net/10138/21914","source":{"id":"https://openalex.org/S4210213322","display_name":"Ty\u00f6v\u00e4entutkimus Vuosikirja","issn_l":"0784-1272","issn":["0784-1272","1459-7780"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["ddh","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"doctoralThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10138/21914","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045890332","display_name":"Pirjo Spuul","orcid":"https://orcid.org/0000-0001-9410-721X","observed_orcids":["https://orcid.org/0000-0001-9410-721X"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Pirjo Spuul","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5045890332"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.8618000149726868,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.8618000149726868,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.051500000059604645,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":0.024399999529123306,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/semliki-forest-virus","display_name":"Semliki Forest virus","score":0.9909999966621399},{"id":"https://openalex.org/keywords/alphavirus","display_name":"alphavirus","score":0.7350000143051147},{"id":"https://openalex.org/keywords/viral-replication-complex","display_name":"viral replication complex","score":0.7940000295639038},{"id":"https://openalex.org/keywords/spherules","display_name":"spherules","score":0.9139999747276306},{"id":"https://openalex.org/keywords/membrane-invagination","display_name":"membrane invagination","score":0.7919999957084656},{"id":"https://openalex.org/keywords/amphipathic-helix","display_name":"amphipathic helix","score":0.8330000042915344},{"id":"https://openalex.org/keywords/membrane-targeting","display_name":"membrane targeting","score":0.5879999995231628},{"id":"https://openalex.org/keywords/homotypic-membrane-fusion","display_name":"homotypic membrane fusion","score":0.7570000290870667}],"concepts":[{"id":"https://openalex.org/C2780963514","wikidata":"https://www.wikidata.org/wiki/Q428413","display_name":"Semliki Forest virus","level":4,"score":0.9337064027786255},{"id":"https://openalex.org/C12590798","wikidata":"https://www.wikidata.org/wiki/Q3933199","display_name":"Replication (statistics)","level":2,"score":0.6343876123428345},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5668649673461914},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.4497600793838501},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4318050742149353},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.4209219813346863},{"id":"https://openalex.org/C140704245","wikidata":"https://www.wikidata.org/wiki/Q3933202","display_name":"Viral replication","level":3,"score":0.4194096624851227},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.09149491786956787},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08350268006324768},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.062134355306625366}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:helda.helsinki.fi:10138/21914","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10138/21914","pdf_url":"http://hdl.handle.net/10138/21914","source":{"id":"https://openalex.org/S4210213322","display_name":"Ty\u00f6v\u00e4entutkimus Vuosikirja","issn_l":"0784-1272","issn":["0784-1272","1459-7780"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["ddh","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"doctoralThesis"},{"id":"mag:61598353","doi":null,"is_oa":false,"landing_page_url":"https://helda.helsinki.fi/bitstream/10138/21914/2/cellular.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:helda.helsinki.fi:10138/21914","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10138/21914","pdf_url":"http://hdl.handle.net/10138/21914","source":{"id":"https://openalex.org/S4210213322","display_name":"Ty\u00f6v\u00e4entutkimus Vuosikirja","issn_l":"0784-1272","issn":["0784-1272","1459-7780"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["ddh","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"doctoralThesis"},"sustainable_development_goals":[{"score":0.5683000087738037,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W61598353.pdf","grobid_xml":"https://content.openalex.org/works/W61598353.grobid-xml"},"referenced_works_count":195,"referenced_works":["https://openalex.org/W162053295","https://openalex.org/W564175456","https://openalex.org/W1257336135","https://openalex.org/W1483657247","https://openalex.org/W1498658961","https://openalex.org/W1509422165","https://openalex.org/W1512392705","https://openalex.org/W1512460569","https://openalex.org/W1525285670","https://openalex.org/W1542581570","https://openalex.org/W1544160017","https://openalex.org/W1551249770","https://openalex.org/W1568604491","https://openalex.org/W1572639795","https://openalex.org/W1573314457","https://openalex.org/W1606156328","https://openalex.org/W1630196113","https://openalex.org/W1638093133","https://openalex.org/W1657414671","https://openalex.org/W1722861930","https://openalex.org/W1735561674","https://openalex.org/W1826069933","https://openalex.org/W1833364882","https://openalex.org/W1855484651","https://openalex.org/W1889186300","https://openalex.org/W1902051377","https://openalex.org/W1903849019","https://openalex.org/W1908565627","https://openalex.org/W1931935783","https://openalex.org/W1957657705","https://openalex.org/W1967519713","https://openalex.org/W1968072008","https://openalex.org/W1970510268","https://openalex.org/W1970541648","https://openalex.org/W1971168306","https://openalex.org/W1971214320","https://openalex.org/W1972488902","https://openalex.org/W1972977209","https://openalex.org/W1973006797","https://openalex.org/W1973742536","https://openalex.org/W1974806147","https://openalex.org/W1977253956","https://openalex.org/W1978293682","https://openalex.org/W1978436940","https://openalex.org/W1979324769","https://openalex.org/W1980609900","https://openalex.org/W1980651773","https://openalex.org/W1982254939","https://openalex.org/W1982260888","https://openalex.org/W1984250381","https://openalex.org/W1985523856","https://openalex.org/W1985767121","https://openalex.org/W1986059049","https://openalex.org/W1987135866","https://openalex.org/W1987462880","https://openalex.org/W1987999872","https://openalex.org/W1989780904","https://openalex.org/W1989930178","https://openalex.org/W1991353963","https://openalex.org/W1991470625","https://openalex.org/W1992987492","https://openalex.org/W1996355353","https://openalex.org/W1996883458","https://openalex.org/W1998079639","https://openalex.org/W1999232604","https://openalex.org/W2000766338","https://openalex.org/W2000928784","https://openalex.org/W2001115857","https://openalex.org/W2001465532","https://openalex.org/W2001826761","https://openalex.org/W2003279213","https://openalex.org/W2003528745","https://openalex.org/W2005132603","https://openalex.org/W2005466661","https://openalex.org/W2007372000","https://openalex.org/W2007818370","https://openalex.org/W2012893948","https://openalex.org/W2013136727","https://openalex.org/W2015132539","https://openalex.org/W2016894006","https://openalex.org/W2017919672","https://openalex.org/W2017998270","https://openalex.org/W2018402225","https://openalex.org/W2021199429","https://openalex.org/W2021663275","https://openalex.org/W2022125636","https://openalex.org/W2024161219","https://openalex.org/W2025290907","https://openalex.org/W2026260520","https://openalex.org/W2026961097","https://openalex.org/W2031220581","https://openalex.org/W2032100189","https://openalex.org/W2032661450","https://openalex.org/W2034377840","https://openalex.org/W2035750069","https://openalex.org/W2037691712","https://openalex.org/W2038382149","https://openalex.org/W2039667276","https://openalex.org/W2040231337","https://openalex.org/W2042969893","https://openalex.org/W2043875683","https://openalex.org/W2043990095","https://openalex.org/W2044320361","https://openalex.org/W2047678584","https://openalex.org/W2049169511","https://openalex.org/W2049227607","https://openalex.org/W2049637624","https://openalex.org/W2050723214","https://openalex.org/W2050922197","https://openalex.org/W2051394809","https://openalex.org/W2052214918","https://openalex.org/W2052505961","https://openalex.org/W2054537646","https://openalex.org/W2054813108","https://openalex.org/W2054853563","https://openalex.org/W2055047899","https://openalex.org/W2058988384","https://openalex.org/W2059530064","https://openalex.org/W2064867196","https://openalex.org/W2065171527","https://openalex.org/W2065717236","https://openalex.org/W2067743388","https://openalex.org/W2067818732","https://openalex.org/W2068106824","https://openalex.org/W2070919514","https://openalex.org/W2073058781","https://openalex.org/W2075069125","https://openalex.org/W2076694007","https://openalex.org/W2078057187","https://openalex.org/W2079046408","https://openalex.org/W2081423576","https://openalex.org/W2083480759","https://openalex.org/W2084754847","https://openalex.org/W2086171773","https://openalex.org/W2086871300","https://openalex.org/W2088632182","https://openalex.org/W2090610404","https://openalex.org/W2090822075","https://openalex.org/W2095928865","https://openalex.org/W2096133555","https://openalex.org/W2098683222","https://openalex.org/W2100029135","https://openalex.org/W2100155449","https://openalex.org/W2101627414","https://openalex.org/W2102610618","https://openalex.org/W2102799805","https://openalex.org/W2103480506","https://openalex.org/W2104152706","https://openalex.org/W2105529329","https://openalex.org/W2106039034","https://openalex.org/W2107250397","https://openalex.org/W2107954865","https://openalex.org/W2108243013","https://openalex.org/W2111755318","https://openalex.org/W2113806981","https://openalex.org/W2115248165","https://openalex.org/W2116640791","https://openalex.org/W2118120234","https://openalex.org/W2118814257","https://openalex.org/W2120345534","https://openalex.org/W2121822849","https://openalex.org/W2122278014","https://openalex.org/W2122550126","https://openalex.org/W2125545448","https://openalex.org/W2125872855","https://openalex.org/W2127658975","https://openalex.org/W2129277563","https://openalex.org/W2132444008","https://openalex.org/W2133706960","https://openalex.org/W2134708393","https://openalex.org/W2136373859","https://openalex.org/W2136997489","https://openalex.org/W2137294277","https://openalex.org/W2138087452","https://openalex.org/W2141322529","https://openalex.org/W2143210808","https://openalex.org/W2144734658","https://openalex.org/W2148769634","https://openalex.org/W2149694036","https://openalex.org/W2149929756","https://openalex.org/W2150120689","https://openalex.org/W2152665657","https://openalex.org/W2156163646","https://openalex.org/W2158603948","https://openalex.org/W2162292304","https://openalex.org/W2162807364","https://openalex.org/W2165402419","https://openalex.org/W2166456692","https://openalex.org/W2168200110","https://openalex.org/W2169274959","https://openalex.org/W2169559002","https://openalex.org/W2171635777","https://openalex.org/W2183670135","https://openalex.org/W2208366175","https://openalex.org/W4299790770"],"related_works":["https://openalex.org/W1606156328","https://openalex.org/W2343372256","https://openalex.org/W2017919672","https://openalex.org/W2784418368","https://openalex.org/W2153161821","https://openalex.org/W7095562429","https://openalex.org/W2064867196","https://openalex.org/W1998951072","https://openalex.org/W2075069125","https://openalex.org/W2140589618"],"abstract_inverted_index":{"All":[0],"positive-strand":[1,50],"RNA":[2,33,41,51,101,129],"viruses":[3,52],"utilize":[4],"cellular":[5,55,189,418],"membranes":[6,72,105,190,196,416],"for":[7,32,37,128,209,216,259,367,402],"the":[8,38,58,61,64,74,77,80,108,125,167,171,181,184,192,195,207,218,222,225,228,232,251,264,267,278,293,298,313,326,347,359,369,372,394,408,429,434],"assembly":[9],"of":[10,60,79,96,170,175,183,194,224,231,244,255,266,328,335,351,364,371,415,433],"their":[11,100,235,338],"replication":[12,68,83,132,151,186,226,261,272,353,373,423,436],"complexes,":[13],"which":[14,141,159,211],"results":[15,243],"in":[16,20,76,102,166,240,250,301,358,376,386,393,421,428],"extensive":[17],"membrane":[18,81,114,280,282,300,348],"modification":[19,193],"infected":[21],"host":[22,43],"cells.":[23],"These":[24],"alterations":[25],"act":[26],"as":[27,137],"structural":[28],"and":[29,73,110,145,191,234,262,305,318,355,390,417,431],"functional":[30],"scaffolds":[31],"replication,":[34],"providing":[35],"protection":[36],"viral":[39,212],"double-stranded":[40],"against":[42],"defences.":[44],"It":[45],"is":[46,142,155,274],"known":[47],"that":[48,66,248],"different":[49,54,384,404],"alter":[53],"membranes.":[56,340],"However,":[57],"origin":[59],"targeted":[62,276],"membranes,":[63],"mechanisms":[65],"direct":[67],"proteins":[69],"to":[70,123,148,179,204,277,312,325,382,425],"specific":[71,126,289],"steps":[75,385],"formation":[78,229,370,430],"bound":[82],"complex":[84,187,273,354,374],"are":[85,135,214,257,285,295],"not":[86],"completely":[87],"understood.":[88],"Alphaviruses":[89],"(including":[90],"Semliki":[91],"Forest":[92],"virus,":[93],"SFV),":[94],"members":[95],"family":[97],"Togaviridae,":[98],"replicate":[99],"association":[103,182],"with":[104,188,320,333],"derived":[106],"from":[107,297],"endosomal":[109],"lysosomal":[111],"compartment,":[112],"inducing":[113,217],"invaginations":[115],"called":[116],"spherules.":[117,219],"Spherule":[118],"structures":[119],"have":[120],"been":[121],"shown":[122],"be":[124,400],"sites":[127],"synthesis.":[130],"Four":[131],"proteins,":[133],"nsP1-nsP4,":[134],"translated":[136],"a":[138,149,288],"polyprotein":[139,268],"(P1234)":[140],"processed":[143],"autocatalytically":[144],"gives":[146],"rise":[147],"membrane-bound":[150],"complex.":[152,437],"Membrane":[153],"binding":[154,252,349],"mediated":[156],"via":[157,307],"nsP1":[158,256],"possesses":[160],"an":[161,308],"amphipathic":[162],"\u03b1-helix":[163],"(binding":[164],"peptide)":[165],"central":[168],"region":[169,254],"protein.":[172],"The":[173,242,271],"aim":[174],"this":[176,377],"thesis":[177,378],"was":[178,202],"characterize":[180],"SFV":[185,352,387,422],"during":[197],"virus":[198,260,360],"infection.":[199],"Therefore,":[200],"it":[201],"necessary":[203],"set":[205],"up":[206,412],"system":[208,366,398],"determining":[210],"components":[213,419],"needed":[215],"In":[220,406],"addition,":[221,407],"targeting":[223],"complex,":[227],"site":[230],"spherules":[233,294,336],"intracellular":[236],"trafficking":[237],"were":[238],"studied":[239],"detail.":[241],"current":[245,409],"work":[246,342,410],"demonstrate":[247],"mutations":[249],"peptide":[253],"lethal":[258],"change":[263],"localization":[265],"precursor":[269],"P123.":[270],"first":[275],"plasma":[279,299],"where":[281],"invaginations,":[283],"spherules,":[284],"induced.":[286],"Using":[287],"regulated":[290],"endocytosis":[291],"event":[292],"internalized":[296],"neutral":[302],"carrier":[303],"vesicles":[304],"transported":[306],"actin-and":[309],"microtubule-dependent":[310],"manner":[311],"pericentriolar":[314],"area.":[315],"Homotypic":[316],"fusions":[317,319],"pre-existing":[321],"acidic":[322],"organelles":[323],"lead":[324],"maturation":[327],"previously":[329],"described":[330,375],"cytopathic":[331],"vacuoles":[332],"hundreds":[334],"on":[337],"limiting":[339],"This":[341,396],"provides":[343],"new":[344,413],"insights":[345],"into":[346],"mechanism":[350],"its":[356],"role":[357],"life":[361,388],"cycle.":[362],"Development":[363],"plasmid-driven":[365],"studying":[368],"allows":[379],"various":[380],"applications":[381],"address":[383],"cycle":[389],"virus-host":[391],"interactions":[392],"future.":[395],"trans-replication":[397],"could":[399],"applied":[401],"many":[403],"viruses.":[405],"brings":[411],"aspects":[414],"involved":[420],"leading":[424],"further":[426],"understanding":[427],"dynamics":[432],"membrane-associated":[435]},"counts_by_year":[{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}