{"id":"https://openalex.org/W607903967","doi":null,"title":"Gold nanoparticle-DNA conjugates for oligonucleotide vectorization towards gene silencing","display_name":"Gold nanoparticle-DNA conjugates for oligonucleotide vectorization towards gene silencing","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W607903967","mag":"607903967"},"language":"en","primary_location":{"id":"pmh:oai:run.unl.pt:10362/6212","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/6212","pdf_url":"http://hdl.handle.net/10362/6212","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10362/6212","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032050597","display_name":"Carina Almeida","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Carina Almeida","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5032050597"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9520999789237976,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9520999789237976,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.021800000220537186,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.013799999840557575,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gold-nanoparticles","display_name":"gold nanoparticles","score":0.9929999709129333},{"id":"https://openalex.org/keywords/triplex-dna","display_name":"triplex DNA","score":0.7110000252723694},{"id":"https://openalex.org/keywords/gene-silencing","display_name":"gene silencing","score":0.7929999828338623},{"id":"https://openalex.org/keywords/laser-heating","display_name":"laser heating","score":0.44200000166893005},{"id":"https://openalex.org/keywords/dna-functionalization","display_name":"DNA functionalization","score":0.5270000100135803},{"id":"https://openalex.org/keywords/fluorescence-resonance-energy-transfer","display_name":"fluorescence resonance energy transfer","score":0.4690000116825104},{"id":"https://openalex.org/keywords/melting-point","display_name":"melting point","score":0.7080000042915344},{"id":"https://openalex.org/keywords/gene-expression-inhibition","display_name":"gene expression inhibition","score":0.6389999985694885},{"id":"https://openalex.org/keywords/in-vitro-transcription-and-translation","display_name":"in vitro transcription and translation","score":0.7129999995231628}],"concepts":[{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.8151556253433228},{"id":"https://openalex.org/C41681595","wikidata":"https://www.wikidata.org/wiki/Q7917855","display_name":"Vectorization (mathematics)","level":2,"score":0.7826253175735474},{"id":"https://openalex.org/C197336794","wikidata":"https://www.wikidata.org/wiki/Q5161150","display_name":"Conjugate","level":2,"score":0.6236509084701538},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5793248414993286},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.5601248145103455},{"id":"https://openalex.org/C104819515","wikidata":"https://www.wikidata.org/wiki/Q900961","display_name":"Colloidal gold","level":3,"score":0.5476922988891602},{"id":"https://openalex.org/C155672457","wikidata":"https://www.wikidata.org/wiki/Q61231","display_name":"Nanoparticle","level":2,"score":0.5422114133834839},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4747500717639923},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.40542274713516235},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3616427779197693},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.35673046112060547},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.34370195865631104},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3317528963088989},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.2827606201171875},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.20597496628761292},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.09633561968803406},{"id":"https://openalex.org/C173608175","wikidata":"https://www.wikidata.org/wiki/Q232661","display_name":"Parallel computing","level":1,"score":0.07763510942459106},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:run.unl.pt:10362/6212","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/6212","pdf_url":"http://hdl.handle.net/10362/6212","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},{"id":"mag:607903967","doi":null,"is_oa":false,"landing_page_url":"https://run.unl.pt/bitstream/10362/6212/1/Almeida_2011.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:run.unl.pt:10362/6212","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/6212","pdf_url":"http://hdl.handle.net/10362/6212","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W607903967.pdf","grobid_xml":"https://content.openalex.org/works/W607903967.grobid-xml"},"referenced_works_count":89,"referenced_works":["https://openalex.org/W231234596","https://openalex.org/W277569163","https://openalex.org/W1518586105","https://openalex.org/W1589258126","https://openalex.org/W1604428153","https://openalex.org/W1906181797","https://openalex.org/W1964146045","https://openalex.org/W1967413924","https://openalex.org/W1968021883","https://openalex.org/W1968202919","https://openalex.org/W1968567751","https://openalex.org/W1969464729","https://openalex.org/W1973579525","https://openalex.org/W1973640908","https://openalex.org/W1979884986","https://openalex.org/W1987147367","https://openalex.org/W1988177186","https://openalex.org/W1989195046","https://openalex.org/W1989454102","https://openalex.org/W1989822610","https://openalex.org/W1995057502","https://openalex.org/W1995734581","https://openalex.org/W1995950979","https://openalex.org/W2003801012","https://openalex.org/W2004396117","https://openalex.org/W2009993736","https://openalex.org/W2014468482","https://openalex.org/W2015440560","https://openalex.org/W2017380185","https://openalex.org/W2021713080","https://openalex.org/W2031432800","https://openalex.org/W2031662478","https://openalex.org/W2033885096","https://openalex.org/W2034002429","https://openalex.org/W2039233074","https://openalex.org/W2040933302","https://openalex.org/W2042551540","https://openalex.org/W2049628338","https://openalex.org/W2052312685","https://openalex.org/W2055222641","https://openalex.org/W2055242795","https://openalex.org/W2055928878","https://openalex.org/W2055944585","https://openalex.org/W2059248731","https://openalex.org/W2059638803","https://openalex.org/W2060502007","https://openalex.org/W2062498920","https://openalex.org/W2065185078","https://openalex.org/W2066468809","https://openalex.org/W2073658360","https://openalex.org/W2073691911","https://openalex.org/W2077863403","https://openalex.org/W2078211790","https://openalex.org/W2086696748","https://openalex.org/W2088014043","https://openalex.org/W2090913926","https://openalex.org/W2091883424","https://openalex.org/W2092347182","https://openalex.org/W2093279818","https://openalex.org/W2094005944","https://openalex.org/W2094938672","https://openalex.org/W2096071764","https://openalex.org/W2096618772","https://openalex.org/W2097709285","https://openalex.org/W2099412187","https://openalex.org/W2102686303","https://openalex.org/W2105541749","https://openalex.org/W2106853492","https://openalex.org/W2107435981","https://openalex.org/W2119457682","https://openalex.org/W2119970960","https://openalex.org/W2120183510","https://openalex.org/W2122935177","https://openalex.org/W2123017921","https://openalex.org/W2128816083","https://openalex.org/W2137756244","https://openalex.org/W2138436760","https://openalex.org/W2149933383","https://openalex.org/W2151399844","https://openalex.org/W2158390129","https://openalex.org/W2164071029","https://openalex.org/W2165719898","https://openalex.org/W2166915947","https://openalex.org/W2167278675","https://openalex.org/W2168052283","https://openalex.org/W2171458052","https://openalex.org/W2323270466","https://openalex.org/W2329345175","https://openalex.org/W2480766469"],"related_works":["https://openalex.org/W2943852895","https://openalex.org/W7132366209","https://openalex.org/W4231668218","https://openalex.org/W624910494","https://openalex.org/W7011642632","https://openalex.org/W7009755285","https://openalex.org/W2950624643","https://openalex.org/W2951293912","https://openalex.org/W329171683","https://openalex.org/W7135297531"],"abstract_inverted_index":{"The":[0,30,147,233],"main":[1],"objective":[2],"of":[3,20,27,37,65,79,95,104,140,149,164,170,180,199,204,223,226,228],"the":[4,21,35,46,63,72,77,87,96,102,105,116,120,135,150,156,165,168,178,197,202,221,224,252,258],"work":[5],"presented":[6],"in":[7,115,138,145,201,248,268,269],"this":[8,181,229],"thesis":[9,121],"was":[10,245,261],"to":[11,48,89,237,263,278],"develop":[12],"a":[13,38,42,57],"gene":[14,64,110,132,266],"silencing":[15,62,111],"system":[16],"by":[17,92],"taking":[18],"advantage":[19],"nanovectorization":[22],"capability":[23],"and":[24,108,128,131,173,190,272,280],"optical":[25],"properties":[26],"gold":[28,97,238],"nanoparticles.":[29],"idea":[31],"is":[32],"based":[33],"on":[34],"construction":[36],"DNA":[39,58,235],"structure":[40,126,152,214],"containing":[41],"therapeutic":[43,106,205,259],"oligonucleotide":[44,107,206,230,260],"with":[45,53,86,208,275],"ability":[47,88],"form":[49],"Hoogsteen":[50,67,171],"hydrogen":[51],"bonds":[52,172],"double-stranded":[54,234],"DNA,":[55],"producing":[56],"triple":[59],"helix,":[60],"besides":[61],"interest.":[66],"bonds,":[68,75],"more":[69],"unstable":[70],"than":[71],"conventional":[73],"Watson-Crick":[74],"permit":[76],"achievement":[78],"lower":[80],"melting":[81,158,192,218],"temperatures.":[82],"This":[83],"attribute,":[84],"coupled":[85],"generate":[90],"heat":[91],"laser":[93],"irradiation":[94],"nanoparticles":[98,239],"used,":[99],"will":[100],"allow":[101],"release":[103,227],"subsequent":[109],"without":[112],"significant":[113],"increase":[114],"medium\u2019s":[117],"temperature.":[118],"Thus,":[119],"comprises":[122],"three":[123,253],"major":[124],"sections:":[125],"design":[127,148],"formation,":[129],"vectorization,":[130],"expression":[133,267],"silencing;":[134],"tasks":[136],"involved":[137],"each":[139],"these":[141],"sections":[142],"were":[143,185,255],"conducted":[144],"parallel.":[146],"obtained":[151],"took":[153],"into":[154],"account":[155],"desired":[157],"temperature,":[159],"stability":[160],"at":[161],"physiological":[162],"conditions":[163],"sequence-forming":[166],"nucleotides,":[167],"number":[169],"ionic":[174],"conditions.":[175],"To":[176],"evaluate":[177],"formation":[179],"structure,":[182],"spectroscopic":[183],"techniques":[184],"mainly":[186],"used:":[187],"FRET":[188],"analysis":[189],"ultraviolet":[191],"curves.":[193],"Both":[194],"approaches":[195],"allowed":[196,220],"identification":[198],"interactions":[200],"presence":[203],"compared":[207],"its":[209],"absence,":[210],"which":[211],"may":[212],"indicate":[213],"formation.":[215],"In":[216],"addition,":[217],"curves":[219],"determination":[222],"temperature":[225],"\u2013":[231],"40\u00baC.":[232],"functionalization":[236],"has":[240],"been":[241],"achieved,":[242],"but":[243],"there":[244],"no":[246],"difference":[247],"electrophoretic":[249],"migration":[250],"when":[251],"oligonucleotides":[254],"present.":[256],"However,":[257],"able":[262],"efficiently":[264],"inhibit":[265],"vitro":[270],"transcription":[271],"translation":[273],"assays":[274],"efficiency":[276],"up":[277],"95%":[279],"60%":[281],"respectively.":[282]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}
