{"id":"https://openalex.org/W59602671","doi":"https://doi.org/10.2144/97234st06","title":"Specific Inhibition of PCR by Non-Extendable Oligonucleotides Using a 5\u2032 to 3\u2032 Exonuclease-Deficient DNA Polymerase","display_name":"Specific Inhibition of PCR by Non-Extendable Oligonucleotides Using a 5\u2032 to 3\u2032 Exonuclease-Deficient DNA Polymerase","publication_year":1997,"publication_date":"1997-10-01","ids":{"openalex":"https://openalex.org/W59602671","doi":"https://doi.org/10.2144/97234st06","mag":"59602671","pmid":"https://pubmed.ncbi.nlm.nih.gov/9343698"},"language":"en","primary_location":{"id":"doi:10.2144/97234st06","doi":"https://doi.org/10.2144/97234st06","is_oa":true,"landing_page_url":"https://doi.org/10.2144/97234st06","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.2144/97234st06","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101680112","display_name":"Dan Yu","orcid":"https://orcid.org/0009-0005-4905-0484","observed_orcids":["https://orcid.org/0009-0005-4905-0484","https://orcid.org/0009-0009-5738-6710","https://orcid.org/0000-0002-1720-3432"]},"institutions":[{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dan Yu","raw_affiliation_strings":["State University of New York at Stony Brook, Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State University of New York at Stony Brook, Stony Brook, NY, USA","institution_ids":["https://openalex.org/I59553526"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Masaya Mukai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Masaya Mukai","raw_affiliation_strings":["State University of New York at Stony Brook, Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State University of New York at Stony Brook, Stony Brook, NY, USA","institution_ids":["https://openalex.org/I59553526"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101976451","display_name":"Qingli Liu","orcid":"https://orcid.org/0009-0007-4665-376X","observed_orcids":["https://orcid.org/0009-0007-4665-376X"]},"institutions":[{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Qingli Liu","raw_affiliation_strings":["State University of New York at Stony Brook, Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State University of New York at Stony Brook, Stony Brook, NY, USA","institution_ids":["https://openalex.org/I59553526"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016332917","display_name":"Charles R. Steinman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Charles R. Steinman","raw_affiliation_strings":["State University of New York at Stony Brook, Stony Brook, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State University of New York at Stony Brook, Stony Brook, NY, USA","institution_ids":["https://openalex.org/I59553526"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I59553526"],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":0.0992,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.32266546,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"23","issue":"4","first_page":"714","last_page":"720"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9663000106811523,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9663000106811523,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.024299999698996544,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pcr-inhibition","display_name":"PCR inhibition","score":0.9399999976158142},{"id":"https://openalex.org/keywords/taq-polymerase","display_name":"Taq DNA polymerase","score":0.9670000076293945},{"id":"https://openalex.org/keywords/primer-extension","display_name":"primer extension","score":0.6840000152587891},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.871999979019165},{"id":"https://openalex.org/keywords/bacillus-subtilis","display_name":"Bacillus subtilis","score":1.0},{"id":"https://openalex.org/keywords/neisseria-gonorrhoeae","display_name":"Neisseria gonorrhoeae","score":0.9950000047683716}],"concepts":[{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.7078632116317749},{"id":"https://openalex.org/C2777501222","wikidata":"https://www.wikidata.org/wiki/Q411664","display_name":"Exonuclease","level":4,"score":0.6840707063674927},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.6403172016143799},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.6041010618209839},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5590953826904297},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5366968512535095},{"id":"https://openalex.org/C28134486","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Applications of PCR","level":5,"score":0.5036055445671082},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.4855806231498718},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.47317177057266235},{"id":"https://openalex.org/C54415193","wikidata":"https://www.wikidata.org/wiki/Q424989","display_name":"Klenow fragment","level":5,"score":0.4409276247024536},{"id":"https://openalex.org/C2781243718","wikidata":"https://www.wikidata.org/wiki/Q5420625","display_name":"Exonuclease III","level":4,"score":0.4159048795700073},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3913380205631256},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.2613283395767212},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.253400981426239},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.062433868646621704}],"mesh":[{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005090","descriptor_name":"Exodeoxyribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009344","descriptor_name":"Neisseria gonorrhoeae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019914","descriptor_name":"Taq Polymerase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D043211","descriptor_name":"Exodeoxyribonuclease V","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.2144/97234st06","doi":"https://doi.org/10.2144/97234st06","is_oa":true,"landing_page_url":"https://doi.org/10.2144/97234st06","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"pmid:9343698","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9343698","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Research Support, Non-U.S. Gov't"},{"id":"pmh:oai:doaj.org/article:1c409085ae9c4e27977ebde1f98a2f86","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/1c409085ae9c4e27977ebde1f98a2f86","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 23, Iss 4, Pp 714-720 (1997)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.2144/97234st06","doi":"https://doi.org/10.2144/97234st06","is_oa":true,"landing_page_url":"https://doi.org/10.2144/97234st06","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2134779779","display_name":"EUBACTERIAL INFECTION IN IDIOPATHIC HUMAN DISEASE","funder_award_id":"R01AR039939","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G4052703792","display_name":"ETIOLOGY OF RHEUMATOID ARTHRITIS","funder_award_id":"R01AR033278","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"}],"funders":[{"id":"https://openalex.org/F4320337362","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":2,"referenced_works":["https://openalex.org/W2070871769","https://openalex.org/W2137295250"],"related_works":["https://openalex.org/W4319165005","https://openalex.org/W1208025800","https://openalex.org/W2002496929","https://openalex.org/W2020273991","https://openalex.org/W2087777856","https://openalex.org/W71565974","https://openalex.org/W2195193764","https://openalex.org/W7101002003","https://openalex.org/W2807009804","https://openalex.org/W2112725402"],"abstract_inverted_index":{"The":[0],"Stoffel":[1],"fragment":[2,30],"of":[3,55,69,73,95,110,116,122,128,148,169,204],"Taq":[4,156],"DNA":[5,18,157],"polymerase":[6],"lacks":[7],"the":[8,32,49,92,117,123,129,177,184],"5'":[9],"to":[10,91,107,175,183,198],"3'":[11],"exonuclease":[12],"activity":[13],"that":[14,179,192],"hydrolyzes":[15],"potentially":[16],"blocking":[17,160],"strands":[19],"during":[20],"primer":[21],"extension.":[22],"We":[23],"there-fore":[24],"asked":[25],"whether":[26],"by":[27,137],"using":[28,201,205],"this":[29],"in":[31,40,150],"PCR,":[33],"non-extendable,":[34],"base-paired":[35],"oligonucleotides":[36,85],"could":[37,212],"inhibit":[38],"amplification":[39,109,188],"a":[41,75,151],"sequence-dependent":[42],"manner.":[43],"Model":[44],"targets":[45,120,126,149],"were":[46,86,104,173],"chosen":[47],"from":[48,79],"partially":[50],"conserved":[51],"ribosomal":[52],"16S":[53],"rDNA":[54,172],"three":[56],"bacterial":[57],"species:":[58],"E.":[59,96],"coli,":[60],"Bacillus":[61],"subtilis":[62],"and":[63,98,164],"Neisseria":[64],"gonorrhoea.":[65],"A":[66],"single":[67,152],"pair":[68],"primers":[70,202],"was":[71,134,144,161],"capable":[72,203],"amplifying":[74],"homologous":[76],"240-bp":[77],"region":[78],"all":[80],"three.":[81],"Two":[82],"non-extendable":[83],"\"blocking\"":[84],"synthesized":[87],"with":[88,146],"sequences":[89],"complementary":[90,112],"inter-primer":[93,185],"regions":[94,168],"coli":[97],"B.":[99],"subtilis,":[100],"respectively.":[101],"Both":[102],"blockers":[103,165,180],"shown":[105],"specifically":[106],"prevent":[108],"their":[111],"targets,":[113,207],"but":[114],"not":[115],"reciprocal":[118,124],"control":[119,125],"or":[121,127],"non-complementary":[130],"N.":[131,170],"gonorrhea.":[132],"Specificity":[133],"further":[135],"confirmed":[136],"an":[138],"internal":[139],"positive":[140],"control.":[141],"Similar":[142],"inhibition":[143,211],"seen":[145],"mixtures":[147],"reaction.":[153],"With":[154],"intact":[155],"polymerase,":[158],"no":[159],"observed.":[162],"Primers":[163],"targeting":[166],"specific":[167],"gonorrhoea":[171],"used":[174],"confirm":[176],"requirement":[178],"be":[181,196,213],"directed":[182],"region.":[186],"Sequence-dependent":[187],"inhibition,":[189],"such":[190,209],"as":[191],"demonstrated":[193],"here,":[194],"would":[195],"applicable":[197],"PCR-related":[199],"strategies":[200],"multiple":[206],"where":[208],"selective":[210],"useful.":[214]},"counts_by_year":[{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}
