{"id":"https://openalex.org/W59372","doi":"https://doi.org/10.1042/bj2860793","title":"Decreased affinity of recombinant antithrombin for heparin due to increased glycosylation","display_name":"Decreased affinity of recombinant antithrombin for heparin due to increased glycosylation","publication_year":1992,"publication_date":"1992-09-15","ids":{"openalex":"https://openalex.org/W59372","doi":"https://doi.org/10.1042/bj2860793","mag":"59372","pmid":"https://pubmed.ncbi.nlm.nih.gov/1417738","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1132973"},"language":"en","primary_location":{"id":"doi:10.1042/bj2860793","doi":"https://doi.org/10.1042/bj2860793","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj2860793","pdf_url":"https://portlandpress.com/biochemj/article-pdf/286/3/793/608166/bj2860793.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://portlandpress.com/biochemj/article-pdf/286/3/793/608166/bj2860793.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111442019","display_name":"Ingemar Bj\u00f6rk","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I298625061","display_name":"Swedish University of Agricultural Sciences","ror":"https://ror.org/02yy8x990","country_code":"SE","type":"education","lineage":["https://openalex.org/I298625061"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"I Bj\u00f6rk","raw_affiliation_strings":["Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala Biomedical Center, Box 575, S-751 23 Uppsala, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala Biomedical Center, Box 575, S-751 23 Uppsala, Sweden","institution_ids":["https://openalex.org/I298625061"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036551400","display_name":"Karin Ylinenj\u00e4rvi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I298625061","display_name":"Swedish University of Agricultural Sciences","ror":"https://ror.org/02yy8x990","country_code":"SE","type":"education","lineage":["https://openalex.org/I298625061"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"K Ylinenj\u00e4rvi","raw_affiliation_strings":["Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala Biomedical Center, Box 575, S-751 23 Uppsala, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala Biomedical Center, Box 575, S-751 23 Uppsala, Sweden","institution_ids":["https://openalex.org/I298625061"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055990354","display_name":"Steven T. Olson","orcid":"https://orcid.org/0000-0002-6083-7510","observed_orcids":["https://orcid.org/0000-0002-6083-7510"]},"institutions":[{"id":"https://openalex.org/I154057602","display_name":"Henry Ford Health","ror":"https://ror.org/02kwnkm68","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I154057602"]},{"id":"https://openalex.org/I2803043754","display_name":"Henry Ford Hospital","ror":"https://ror.org/0193sb042","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I154057602","https://openalex.org/I2803043754"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S T Olson","raw_affiliation_strings":["Division of Biochemical Research, Henry Ford Hospital, 2799 W. Grand Boulevard, Detroit, MI 48202, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biochemical Research, Henry Ford Hospital, 2799 W. Grand Boulevard, Detroit, MI 48202, U.S.A","institution_ids":["https://openalex.org/I154057602","https://openalex.org/I2803043754"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028154637","display_name":"Peter Hermentin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210105121","display_name":"Behringwerke (Germany)","ror":"https://ror.org/01647vc39","country_code":"DE","type":"company","lineage":["https://openalex.org/I4210105121"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"P Hermentin","raw_affiliation_strings":["Research Laboratories of Behringwerke AG, P.O. Box 1140, D-3550 Marburg/Lahn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Laboratories of Behringwerke AG, P.O. Box 1140, D-3550 Marburg/Lahn, Germany","institution_ids":["https://openalex.org/I4210105121"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068457915","display_name":"Harald S. Conradt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210124929","display_name":"Helmholtz Centre for Infection Research","ror":"https://ror.org/03d0p2685","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I4210124929"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"H S Conradt","raw_affiliation_strings":["Gesellschaft f\u00fcr Biotechnologische Forschung, Mascheroder Weg 1, D-3300 Braunschweig, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gesellschaft f\u00fcr Biotechnologische Forschung, Mascheroder Weg 1, D-3300 Braunschweig, Germany","institution_ids":["https://openalex.org/I4210124929"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088878291","display_name":"Gerd Zettlmei\u00dfl","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210105121","display_name":"Behringwerke (Germany)","ror":"https://ror.org/01647vc39","country_code":"DE","type":"company","lineage":["https://openalex.org/I4210105121"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"G Zettlmeissl","raw_affiliation_strings":["Research Laboratories of Behringwerke AG, P.O. Box 1140, D-3550 Marburg/Lahn, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Laboratories of Behringwerke AG, P.O. Box 1140, D-3550 Marburg/Lahn, Germany","institution_ids":["https://openalex.org/I4210105121"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.6123,"has_fulltext":true,"cited_by_count":55,"citation_normalized_percentile":{"value":0.88444409,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"286","issue":"3","first_page":"793","last_page":"800"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.3296000063419342,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.3296000063419342,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.23360000550746918,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.19900000095367432,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heparin-affinity","display_name":"heparin affinity","score":0.9100000262260437},{"id":"https://openalex.org/keywords/glycosylation","display_name":"glycosylation","score":0.8289999961853027},{"id":"https://openalex.org/keywords/n-glycans","display_name":"N-glycans","score":0.9120000004768372},{"id":"https://openalex.org/keywords/biantennary-oligosaccharides","display_name":"biantennary oligosaccharides","score":0.9660000205039978},{"id":"https://openalex.org/keywords/thrombin-inhibition","display_name":"thrombin inhibition","score":0.6740000247955322},{"id":"https://openalex.org/keywords/heparin-binding-site","display_name":"heparin-binding site","score":0.7760000228881836},{"id":"https://openalex.org/keywords/cho-cells","display_name":"CHO cells","score":0.6420000195503235},{"id":"https://openalex.org/keywords/bhk-cells","display_name":"BHK cells","score":0.9789999723434448},{"id":"https://openalex.org/keywords/deglycosylation","display_name":"deglycosylation","score":0.8759999871253967},{"id":"https://openalex.org/keywords/protein-heterogeneity","display_name":"protein heterogeneity","score":0.5799999833106995}],"concepts":[{"id":"https://openalex.org/C2779026020","wikidata":"https://www.wikidata.org/wiki/Q416336","display_name":"Antithrombin","level":3,"score":0.8854392766952515},{"id":"https://openalex.org/C2777557582","wikidata":"https://www.wikidata.org/wiki/Q190016","display_name":"Heparin","level":2,"score":0.7616830468177795},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.7424342632293701},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.7270761132240295},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.682542085647583},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5622835159301758},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5440970063209534},{"id":"https://openalex.org/C2777292125","wikidata":"https://www.wikidata.org/wiki/Q409166","display_name":"Thrombin","level":3,"score":0.5421676635742188},{"id":"https://openalex.org/C33263850","wikidata":"https://www.wikidata.org/wiki/Q4838438","display_name":"Baby hamster kidney cell","level":3,"score":0.5320855975151062},{"id":"https://openalex.org/C2778575167","wikidata":"https://www.wikidata.org/wiki/Q6573","display_name":"Hamster","level":2,"score":0.5147964358329773},{"id":"https://openalex.org/C2777313579","wikidata":"https://www.wikidata.org/wiki/Q898365","display_name":"Glycosylation","level":2,"score":0.4851975739002228},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3874436020851135},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.21520301699638367},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.16099610924720764},{"id":"https://openalex.org/C89560881","wikidata":"https://www.wikidata.org/wiki/Q101026","display_name":"Platelet","level":2,"score":0.14370200037956238},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.142306387424469},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.08673658967018127},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.0656629204750061},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.06484004855155945},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000991","descriptor_name":"Antithrombins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005453","descriptor_name":"Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006493","descriptor_name":"Heparin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009844","descriptor_name":"Oligosaccharides","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1042/bj2860793","doi":"https://doi.org/10.1042/bj2860793","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj2860793","pdf_url":"https://portlandpress.com/biochemj/article-pdf/286/3/793/608166/bj2860793.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1132973","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1132973","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem J","raw_type":"Text"},{"id":"pmid:1417738","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1417738","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Biochemical journal","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1042/bj2860793","doi":"https://doi.org/10.1042/bj2860793","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj2860793","pdf_url":"https://portlandpress.com/biochemj/article-pdf/286/3/793/608166/bj2860793.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.7246000170707703,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2519008473","display_name":null,"funder_award_id":"HL 39888","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W59372.pdf","grobid_xml":"https://content.openalex.org/works/W59372.grobid-xml"},"referenced_works_count":52,"referenced_works":["https://openalex.org/W84979909","https://openalex.org/W1488573465","https://openalex.org/W1497028439","https://openalex.org/W1499963171","https://openalex.org/W1514571277","https://openalex.org/W1525638648","https://openalex.org/W1528449587","https://openalex.org/W1534757774","https://openalex.org/W1537777030","https://openalex.org/W1539046821","https://openalex.org/W1543914169","https://openalex.org/W1563465184","https://openalex.org/W1563674350","https://openalex.org/W1574724167","https://openalex.org/W1577984877","https://openalex.org/W1581586115","https://openalex.org/W1582894915","https://openalex.org/W1584663505","https://openalex.org/W1595277218","https://openalex.org/W1608901332","https://openalex.org/W1618720360","https://openalex.org/W1664950994","https://openalex.org/W1926739401","https://openalex.org/W1989125896","https://openalex.org/W1992551721","https://openalex.org/W1994305199","https://openalex.org/W1994477505","https://openalex.org/W1996658025","https://openalex.org/W1998023794","https://openalex.org/W2004303300","https://openalex.org/W2004724393","https://openalex.org/W2009929707","https://openalex.org/W2014281268","https://openalex.org/W2018289835","https://openalex.org/W2020631948","https://openalex.org/W2031119708","https://openalex.org/W2038363212","https://openalex.org/W2051797083","https://openalex.org/W2069112575","https://openalex.org/W2073474412","https://openalex.org/W2089341748","https://openalex.org/W2097447907","https://openalex.org/W2100160042","https://openalex.org/W2100837269","https://openalex.org/W2110981947","https://openalex.org/W2111301002","https://openalex.org/W2122748572","https://openalex.org/W2396342198","https://openalex.org/W2521914066","https://openalex.org/W4206206536","https://openalex.org/W6631194830","https://openalex.org/W6832386920"],"related_works":["https://openalex.org/W1964816512","https://openalex.org/W1919050777","https://openalex.org/W1489224366","https://openalex.org/W2412130670","https://openalex.org/W2062596317","https://openalex.org/W2036286114","https://openalex.org/W2144247419","https://openalex.org/W2016107045","https://openalex.org/W2338482884","https://openalex.org/W2043360085"],"abstract_inverted_index":{"Recombinant":[0],"antithrombin":[1,48,110,128,231,283],"produced":[2,232],"by":[3,24,149,233,284],"baby":[4],"hamster":[5,10],"kidney":[6],"(BHK)":[7],"or":[8,102,190],"Chinese":[9],"ovary":[11],"(CHO)":[12],"cells":[13,207],"was":[14,62,147],"separated":[15],"into":[16],"two":[17,235],"fractions,":[18],"containing":[19],"comparable":[20],"amounts":[21],"of":[22,46,56,71,75,77,98,106,153,168,194,212,218,247,251,262,270,281],"protein,":[23],"affinity":[25,42,55,88,115,125,167,261],"chromatography":[26],"on":[27],"matrix-linked":[28,178],"heparin.":[29,266],"Fluorescence":[30],"titrations":[31],"showed":[32,80,199],"that":[33,45,81,174,200,228,242],"the":[34,54,57,72,82,94,100,103,121,150,154,159,165,169,185,201,222,229,234,243,252,260,278],"more":[35,58,117],"tightly":[36,187,223],"binding":[37,60,171,188,203,224],"fraction":[38,61,111,122,189,204],"had":[39,208],"a":[40,136,181,209,215,248,256],"heparin":[41,90,114,166,179],"similar":[43,182],"to":[44,184],"plasma":[47,127,191],"(Kd":[49,66],"approximately":[50,67],"20":[51],"nM),":[52],"whereas":[53],"weakly":[59,170,202],"nearly":[63],"10-fold":[64],"lower":[65,113,216],"175":[68],"nM).":[69],"Analyses":[70,193],"heparin-catalysed":[73,104],"rate":[74,96],"inhibition":[76],"thrombin":[78],"further":[79],"fractions":[83],"differed":[84],"only":[85],"in":[86,93,129,255,259],"their":[87],"for":[89,265,273],"and":[91,126,214,241],"not":[92],"intrinsic":[95],"constant":[97],"either":[99],"uncatalysed":[101],"inactivation":[105],"thrombin.":[107],"The":[108],"recombinant":[109,230,282],"with":[112,123,135],"migrated":[116],"slowly":[118],"than":[119,221],"both":[120],"higher":[124,138,210],"SDS/PAGE":[130],"under":[131],"reducing":[132],"conditions,":[133],"consistent":[134],"slightly":[137],"apparent":[139,144],"relative":[140],"molecular":[141],"mass.":[142],"This":[143],"size":[145],"difference":[146],"abolished":[148],"enzymic":[151],"removal":[152,162],"carbohydrate":[155,196,245],"side":[156,197],"chains":[157,198],"from":[158,177,205],"proteins.":[160],"Such":[161],"also":[163],"increased":[164,244],"fraction,":[172],"so":[173],"it":[175],"eluted":[176],"at":[180,276],"position":[183],"deglycosylated":[186],"antithrombin.":[192],"N-linked":[195],"CHO":[206],"proportion":[211,217,250],"tetra-antennary":[213],"biantennary":[219],"oligosaccharides":[220],"fraction.":[225],"We":[226],"conclude":[227],"cell":[236],"lines":[237],"is":[238],"heterogeneously":[239],"glycosylated":[240],"content":[246],"large":[249],"molecules":[253,264],"results":[254],"substantial":[257],"decrease":[258],"these":[263],"These":[267],"findings":[268],"are":[269],"particular":[271],"relevance":[272],"studies":[274],"aimed":[275],"characterizing":[277],"heparin-binding":[279],"site":[280],"site-directed":[285],"mutagenesis.":[286]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}
