{"id":"https://openalex.org/W58911136","doi":"https://doi.org/10.1007/978-1-60327-192-9_9","title":"Methylation Analysis by Microarray","display_name":"Methylation Analysis by Microarray","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W58911136","doi":"https://doi.org/10.1007/978-1-60327-192-9_9","mag":"58911136","pmid":"https://pubmed.ncbi.nlm.nih.gov/19488875","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2838393"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-60327-192-9_9","doi":"https://doi.org/10.1007/978-1-60327-192-9_9","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-60327-192-9_9","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology\u2122","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2478325122","display_name":"Microarray Analysis of the Physical Genome"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2838393/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068012765","display_name":"Daniel E. Deatherage","orcid":"https://orcid.org/0000-0001-8914-8359","observed_orcids":["https://orcid.org/0000-0001-8914-8359"]},"institutions":[{"id":"https://openalex.org/I2799885155","display_name":"The Ohio State University Comprehensive Cancer Center \u2013 Arthur G. James Cancer Hospital and Richard J. Solove Research Institute","ror":"https://ror.org/028t46f04","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799885155","https://openalex.org/I2802841970"]},{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel E. Deatherage","raw_affiliation_strings":["Human Cancer Genetics Program, The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA","The Ohio State University Comprehensive Cancer Center, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Human Cancer Genetics Program, The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA","institution_ids":["https://openalex.org/I2799885155","https://openalex.org/I52357470"]},{"raw_affiliation_string":"The Ohio State University Comprehensive Cancer Center, The Ohio State University","institution_ids":["https://openalex.org/I2799885155","https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000357133","display_name":"Dustin Potter","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2799885155","display_name":"The Ohio State University Comprehensive Cancer Center \u2013 Arthur G. James Cancer Hospital and Richard J. Solove Research Institute","ror":"https://ror.org/028t46f04","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799885155","https://openalex.org/I2802841970"]},{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dustin Potter","raw_affiliation_strings":["Human Cancer Genetics Program, The Ohio State University Comprehensive Cancer Center and the Mathematical Biosciences Institute, The Ohio State University, Columbus, OH, USA","The Ohio State University Comprehensive Cancer Center and the Mathematical Biosciences Institute, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Human Cancer Genetics Program, The Ohio State University Comprehensive Cancer Center and the Mathematical Biosciences Institute, The Ohio State University, Columbus, OH, USA","institution_ids":["https://openalex.org/I2799885155","https://openalex.org/I52357470"]},{"raw_affiliation_string":"The Ohio State University Comprehensive Cancer Center and the Mathematical Biosciences Institute, The Ohio State University","institution_ids":["https://openalex.org/I2799885155","https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019710145","display_name":"Pearlly S. Yan","orcid":"https://orcid.org/0000-0003-1965-4920","observed_orcids":["https://orcid.org/0000-0003-1965-4920"]},"institutions":[{"id":"https://openalex.org/I2799885155","display_name":"The Ohio State University Comprehensive Cancer Center \u2013 Arthur G. James Cancer Hospital and Richard J. Solove Research Institute","ror":"https://ror.org/028t46f04","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799885155","https://openalex.org/I2802841970"]},{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Pearlly S. Yan","raw_affiliation_strings":["Human Cancer Genetics Program, The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA","The Ohio State University Comprehensive Cancer Center, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Human Cancer Genetics Program, The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA","institution_ids":["https://openalex.org/I2799885155","https://openalex.org/I52357470"]},{"raw_affiliation_string":"The Ohio State University Comprehensive Cancer Center, The Ohio State University","institution_ids":["https://openalex.org/I2799885155","https://openalex.org/I52357470"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049119185","display_name":"Tim H.\u2010M. Huang","orcid":"https://orcid.org/0000-0001-5985-9176","observed_orcids":["https://orcid.org/0000-0001-5985-9176"]},"institutions":[{"id":"https://openalex.org/I2799885155","display_name":"The Ohio State University Comprehensive Cancer Center \u2013 Arthur G. James Cancer Hospital and Richard J. Solove Research Institute","ror":"https://ror.org/028t46f04","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I2799885155","https://openalex.org/I2802841970"]},{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tim H.-M. Huang","raw_affiliation_strings":["Human Cancer Genetics Program, The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA","The Ohio State University Comprehensive Cancer Center, The Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Human Cancer Genetics Program, The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA","institution_ids":["https://openalex.org/I2799885155","https://openalex.org/I52357470"]},{"raw_affiliation_string":"The Ohio State University Comprehensive Cancer Center, The Ohio State University","institution_ids":["https://openalex.org/I2799885155","https://openalex.org/I52357470"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091196188","display_name":"Shili Lin","orcid":"https://orcid.org/0000-0003-2467-4491","observed_orcids":["https://orcid.org/0000-0003-2467-4491"]},"institutions":[{"id":"https://openalex.org/I52357470","display_name":"The Ohio State University","ror":"https://ror.org/00rs6vg23","country_code":"US","type":"education","lineage":["https://openalex.org/I52357470"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shili Lin","raw_affiliation_strings":["Department of Statistics and the Mathematical Biosciences Institute, The Ohio State University, Columbus, OH, USA","the Ohio State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Statistics and the Mathematical Biosciences Institute, The Ohio State University, Columbus, OH, USA","institution_ids":["https://openalex.org/I52357470"]},{"raw_affiliation_string":"the Ohio State University","institution_ids":["https://openalex.org/I52357470"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.9475,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.84391577,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"556","issue":null,"first_page":"117","last_page":"139"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.0024999999441206455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11928","display_name":"Genetic Syndromes and Imprinting","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-methylation","display_name":"DNA methylation","score":0.7599999904632568},{"id":"https://openalex.org/keywords/cpg-islands","display_name":"CpG islands","score":0.8650000095367432},{"id":"https://openalex.org/keywords/methylation-sensitive-restriction-enzymes","display_name":"methylation-sensitive restriction enzymes","score":0.8970000147819519},{"id":"https://openalex.org/keywords/dna-microarrays","display_name":"DNA microarrays","score":0.5849999785423279},{"id":"https://openalex.org/keywords/quality-control","display_name":"quality control","score":0.7070000171661377},{"id":"https://openalex.org/keywords/quantitative-image-analysis","display_name":"quantitative image analysis","score":0.9279999732971191},{"id":"https://openalex.org/keywords/background-correction","display_name":"background correction","score":0.9980000257492065}],"concepts":[{"id":"https://openalex.org/C33288867","wikidata":"https://www.wikidata.org/wiki/Q518328","display_name":"Methylation","level":3,"score":0.7006199955940247},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5984513163566589},{"id":"https://openalex.org/C186836561","wikidata":"https://www.wikidata.org/wiki/Q1931516","display_name":"Microarray","level":4,"score":0.5863435864448547},{"id":"https://openalex.org/C190727270","wikidata":"https://www.wikidata.org/wiki/Q874745","display_name":"DNA methylation","level":4,"score":0.5434539318084717},{"id":"https://openalex.org/C2780385302","wikidata":"https://www.wikidata.org/wiki/Q367158","display_name":"Protocol (science)","level":3,"score":0.5363707542419434},{"id":"https://openalex.org/C140173407","wikidata":"https://www.wikidata.org/wiki/Q1138358","display_name":"CpG site","level":5,"score":0.5252040028572083},{"id":"https://openalex.org/C116114238","wikidata":"https://www.wikidata.org/wiki/Q6823960","display_name":"Methylated DNA immunoprecipitation","level":5,"score":0.521784782409668},{"id":"https://openalex.org/C2781148417","wikidata":"https://www.wikidata.org/wiki/Q5533486","display_name":"Genome browser","level":5,"score":0.507628321647644},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.47615763545036316},{"id":"https://openalex.org/C136953436","wikidata":"https://www.wikidata.org/wiki/Q6000286","display_name":"Illumina Methylation Assay","level":5,"score":0.45465824007987976},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.39264246821403503},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.36142265796661377},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.32146602869033813},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.26622071862220764},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17132624983787537},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.14407259225845337},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.06374171376228333},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0571172833442688},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019175","descriptor_name":"DNA Methylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020411","descriptor_name":"Oligonucleotide Array Sequence Analysis","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D020411","descriptor_name":"Oligonucleotide Array Sequence Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1007/978-1-60327-192-9_9","doi":"https://doi.org/10.1007/978-1-60327-192-9_9","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-60327-192-9_9","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology\u2122","raw_type":"book-chapter"},{"id":"pmid:19488875","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19488875","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2838393","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2838393/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Methods Mol Biol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2838393","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC2838393/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Methods Mol Biol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4941813046","display_name":"Interrogating Epigenetic Changes in Cancer Genomes","funder_award_id":"U54CA113001","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W64738384","https://openalex.org/W79377702","https://openalex.org/W1493454437","https://openalex.org/W1495359568","https://openalex.org/W1500523991","https://openalex.org/W1507742524","https://openalex.org/W1570354362","https://openalex.org/W1649167417","https://openalex.org/W1973614663","https://openalex.org/W1982516880","https://openalex.org/W1987782593","https://openalex.org/W2002810210","https://openalex.org/W2040149948","https://openalex.org/W2053478107","https://openalex.org/W2071650644","https://openalex.org/W2096863518","https://openalex.org/W2103994569","https://openalex.org/W2120865735","https://openalex.org/W2130919037","https://openalex.org/W2132921193","https://openalex.org/W2141222206","https://openalex.org/W2141410747","https://openalex.org/W2150926065","https://openalex.org/W2157795344","https://openalex.org/W2163296727","https://openalex.org/W2168991217","https://openalex.org/W2999729612","https://openalex.org/W4237142329","https://openalex.org/W4252684946","https://openalex.org/W4254711206","https://openalex.org/W4294107304","https://openalex.org/W4299681612"],"related_works":["https://openalex.org/W64738384","https://openalex.org/W2166082587","https://openalex.org/W4245947820","https://openalex.org/W183962763","https://openalex.org/W7098376186","https://openalex.org/W7101071627","https://openalex.org/W2147578952","https://openalex.org/W2129265405","https://openalex.org/W2168794860","https://openalex.org/W2430004919"],"abstract_inverted_index":{"Differential":[0],"methylation":[1,8,68],"hybridization":[2],"(DMH)":[3],"is":[4,90],"a":[5,24,46],"high-throughput":[6],"DNA":[7],"screening":[9],"tool":[10],"that":[11,59],"utilizes":[12],"methylation-sensitive":[13],"restriction":[14],"enzymes":[15],"to":[16,23,62],"profile":[17],"methylated":[18],"fragments":[19],"by":[20],"hybridizing":[21],"them":[22],"CpG":[25],"island":[26],"microarray.":[27],"This":[28],"array":[29],"contains":[30],"probes":[31],"spanning":[32],"all":[33],"the":[34,39,71,74],"27,800":[35],"islands":[36],"annotated":[37],"in":[38],"UCSC":[40],"Genome":[41],"Browser.":[42],"Herein":[43],"we":[44,94],"describe":[45],"DMH":[47,88,98],"protocol":[48,89],"with":[49,82],"clearly":[50],"identified":[51,79],"quality":[52,119],"control":[53,75,120],"points.":[54],"In":[55,92],"this":[56],"manner,":[57],"samples":[58,76],"are":[60,121],"unlikely":[61],"provide":[63,95],"good":[64],"read-outs":[65],"for":[66,109],"differential":[67],"profiles":[69],"between":[70],"test":[72],"and":[73,80,106,113],"will":[77],"be":[78],"repeated":[81],"appropriate":[83],"modifications.":[84],"The":[85],"step-by-step":[86],"laboratory":[87],"described.":[91],"addition,":[93],"descriptions":[96],"regarding":[97,118],"data":[99],"analysis,":[100],"including":[101],"image":[102],"quantification,":[103],"background":[104],"correction,":[105],"statistical":[107],"procedures":[108],"both":[110],"exploratory":[111],"analysis":[112],"more":[114],"formal":[115],"inferences.":[116],"Issues":[117],"addressed":[122],"as":[123],"well.":[124]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}