{"id":"https://openalex.org/W58895165","doi":null,"title":"Massively parallel analysis of cells and nucleic acids","display_name":"Massively parallel analysis of cells and nucleic acids","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W58895165","mag":"58895165"},"language":"en","primary_location":{"id":"pmh:oai:DiVA.org:kth-45671","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-45671","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-45671","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017664172","display_name":"Julia Sandberg","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Julia Sandberg","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5017664172"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.22280000150203705,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.22280000150203705,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.21979999542236328,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.21279999613761902,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/single-cell-analysis","display_name":"single-cell analysis","score":0.8960000276565552},{"id":"https://openalex.org/keywords/neuronal-differentiation","display_name":"neuronal differentiation","score":0.7960000038146973},{"id":"https://openalex.org/keywords/pluripotency","display_name":"pluripotency","score":0.8849999904632568},{"id":"https://openalex.org/keywords/emulsion-pcr","display_name":"emulsion PCR","score":0.6710000038146973},{"id":"https://openalex.org/keywords/454-pyrosequencing","display_name":"454 pyrosequencing","score":0.7580000162124634},{"id":"https://openalex.org/keywords/amplicon-sequencing","display_name":"amplicon sequencing","score":0.7429999709129333},{"id":"https://openalex.org/keywords/cell-heterogeneity","display_name":"cellular heterogeneity","score":0.45399999618530273},{"id":"https://openalex.org/keywords/cell-morphology","display_name":"cell morphology","score":0.38999998569488525},{"id":"https://openalex.org/keywords/cell-differentiation","display_name":"cell differentiation","score":0.5860000252723694},{"id":"https://openalex.org/keywords/reprogramming","display_name":"reprogramming","score":0.5580000281333923}],"concepts":[{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.7237319946289062},{"id":"https://openalex.org/C190475519","wikidata":"https://www.wikidata.org/wiki/Q544384","display_name":"Massively parallel","level":2,"score":0.6937870979309082},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5357747673988342},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4363260269165039},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3952130675315857},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.26618698239326477},{"id":"https://openalex.org/C173608175","wikidata":"https://www.wikidata.org/wiki/Q232661","display_name":"Parallel computing","level":1,"score":0.23863372206687927},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18813511729240417}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:DiVA.org:kth-45671","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-45671","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},{"id":"mag:58895165","doi":null,"is_oa":false,"landing_page_url":"http://kth.diva-portal.org/smash/get/diva2:452821/FULLTEXT01.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:DiVA.org:kth-45671","doi":null,"is_oa":true,"landing_page_url":"http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-45671","pdf_url":null,"source":{"id":"https://openalex.org/S4306401559","display_name":"KTH Publication Database DiVA (KTH Royal Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86987016","host_organization_name":"KTH Royal Institute of Technology","host_organization_lineage":["https://openalex.org/I86987016"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/doctoralThesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":313,"referenced_works":["https://openalex.org/W13383624","https://openalex.org/W114395171","https://openalex.org/W183254587","https://openalex.org/W1501017887","https://openalex.org/W1506928604","https://openalex.org/W1552123405","https://openalex.org/W1555016068","https://openalex.org/W1555039120","https://openalex.org/W1575228817","https://openalex.org/W1602222357","https://openalex.org/W1658221551","https://openalex.org/W1701654383","https://openalex.org/W1767380958","https://openalex.org/W1859302788","https://openalex.org/W1925060465","https://openalex.org/W1965175512","https://openalex.org/W1968852642","https://openalex.org/W1968957319","https://openalex.org/W1969267177","https://openalex.org/W1969447706","https://openalex.org/W1969596239","https://openalex.org/W1969757078","https://openalex.org/W1970919055","https://openalex.org/W1971403296","https://openalex.org/W1971680669","https://openalex.org/W1972073219","https://openalex.org/W1973878786","https://openalex.org/W1973950074","https://openalex.org/W1974646727","https://openalex.org/W1975581000","https://openalex.org/W1975687263","https://openalex.org/W1978660097","https://openalex.org/W1979072451","https://openalex.org/W1979440527","https://openalex.org/W1980104332","https://openalex.org/W1980221373","https://openalex.org/W1980388530","https://openalex.org/W1980461160","https://openalex.org/W1980606681","https://openalex.org/W1980873478","https://openalex.org/W1980951162","https://openalex.org/W1981509058","https://openalex.org/W1983477307","https://openalex.org/W1984248485","https://openalex.org/W1984731778","https://openalex.org/W1984783889","https://openalex.org/W1984857185","https://openalex.org/W1986203126","https://openalex.org/W1986474467","https://openalex.org/W1987068563","https://openalex.org/W1987666378","https://openalex.org/W1988146848","https://openalex.org/W1988290769","https://openalex.org/W1988444808","https://openalex.org/W1988834469","https://openalex.org/W1988929438","https://openalex.org/W1989191870","https://openalex.org/W1989215251","https://openalex.org/W1989906952","https://openalex.org/W1990275625","https://openalex.org/W1990601349","https://openalex.org/W1991342096","https://openalex.org/W1991637670","https://openalex.org/W1991742987","https://openalex.org/W1991872434","https://openalex.org/W1992505985","https://openalex.org/W1992595091","https://openalex.org/W1992861885","https://openalex.org/W1993009001","https://openalex.org/W1994057432","https://openalex.org/W1994248362","https://openalex.org/W1995530621","https://openalex.org/W1995616824","https://openalex.org/W1996547052","https://openalex.org/W1996568337","https://openalex.org/W1996811680","https://openalex.org/W1997437810","https://openalex.org/W2000402409","https://openalex.org/W2000894445","https://openalex.org/W2000905326","https://openalex.org/W2001002816","https://openalex.org/W2001662737","https://openalex.org/W2001725958","https://openalex.org/W2005830991","https://openalex.org/W2006645462","https://openalex.org/W2009071040","https://openalex.org/W2010318757","https://openalex.org/W2010847848","https://openalex.org/W2011152694","https://openalex.org/W2012016911","https://openalex.org/W2012142685","https://openalex.org/W2012986985","https://openalex.org/W2013167503","https://openalex.org/W2013590760","https://openalex.org/W2015507028","https://openalex.org/W2016294282","https://openalex.org/W2017350973","https://openalex.org/W2018246047","https://openalex.org/W2019490156","https://openalex.org/W2021729201","https://openalex.org/W2022824615","https://openalex.org/W2023839145","https://openalex.org/W2023885786","https://openalex.org/W2024692616","https://openalex.org/W2024975965","https://openalex.org/W2026710381","https://openalex.org/W2026916707","https://openalex.org/W2028008883","https://openalex.org/W2030365976","https://openalex.org/W2030692069","https://openalex.org/W2031212650","https://openalex.org/W2031722560","https://openalex.org/W2032118018","https://openalex.org/W2032251969","https://openalex.org/W2032518018","https://openalex.org/W2032711662","https://openalex.org/W2036210716","https://openalex.org/W2037064308","https://openalex.org/W2039403461","https://openalex.org/W2041834375","https://openalex.org/W2043935483","https://openalex.org/W2044002275","https://openalex.org/W2044893057","https://openalex.org/W2045071699","https://openalex.org/W2045654196","https://openalex.org/W2047745484","https://openalex.org/W2048352787","https://openalex.org/W2048723772","https://openalex.org/W2050076318","https://openalex.org/W2050163545","https://openalex.org/W2050717506","https://openalex.org/W2051815061","https://openalex.org/W2051824898","https://openalex.org/W2053184946","https://openalex.org/W2054180938","https://openalex.org/W2055214702","https://openalex.org/W2058096164","https://openalex.org/W2058140004","https://openalex.org/W2059188100","https://openalex.org/W2059198040","https://openalex.org/W2059362154","https://openalex.org/W2063866314","https://openalex.org/W2064063117","https://openalex.org/W2064200890","https://openalex.org/W2065213545","https://openalex.org/W2065324308","https://openalex.org/W2065709676","https://openalex.org/W2065999427","https://openalex.org/W2066310505","https://openalex.org/W2066789848","https://openalex.org/W2067280146","https://openalex.org/W2067286104","https://openalex.org/W2067655540","https://openalex.org/W2067737436","https://openalex.org/W2069203904","https://openalex.org/W2069392341","https://openalex.org/W2070181423","https://openalex.org/W2070418129","https://openalex.org/W2070479548","https://openalex.org/W2070583923","https://openalex.org/W2070721758","https://openalex.org/W2071199144","https://openalex.org/W2072094636","https://openalex.org/W2073934376","https://openalex.org/W2075672295","https://openalex.org/W2075922438","https://openalex.org/W2076034830","https://openalex.org/W2076424822","https://openalex.org/W2076961967","https://openalex.org/W2077213730","https://openalex.org/W2077248448","https://openalex.org/W2077926602","https://openalex.org/W2078281201","https://openalex.org/W2078776318","https://openalex.org/W2079286282","https://openalex.org/W2081332370","https://openalex.org/W2081412654","https://openalex.org/W2083674311","https://openalex.org/W2083974946","https://openalex.org/W2087242711","https://openalex.org/W2087464200","https://openalex.org/W2089746511","https://openalex.org/W2089828850","https://openalex.org/W2090308810","https://openalex.org/W2091151261","https://openalex.org/W2091167042","https://openalex.org/W2091290287","https://openalex.org/W2091443952","https://openalex.org/W2092182592","https://openalex.org/W2092600280","https://openalex.org/W2092779913","https://openalex.org/W2093116784","https://openalex.org/W2093331431","https://openalex.org/W2094900722","https://openalex.org/W2095340238","https://openalex.org/W2095875473","https://openalex.org/W2095909724","https://openalex.org/W2096144171","https://openalex.org/W2097097085","https://openalex.org/W2097318087","https://openalex.org/W2097337885","https://openalex.org/W2097348296","https://openalex.org/W2099319506","https://openalex.org/W2101301189","https://openalex.org/W2102753907","https://openalex.org/W2103740144","https://openalex.org/W2104708641","https://openalex.org/W2105776281","https://openalex.org/W2106335939","https://openalex.org/W2108058966","https://openalex.org/W2109178214","https://openalex.org/W2109550992","https://openalex.org/W2109700388","https://openalex.org/W2109847775","https://openalex.org/W2110246558","https://openalex.org/W2111435467","https://openalex.org/W2112304461","https://openalex.org/W2112515280","https://openalex.org/W2113649367","https://openalex.org/W2114752404","https://openalex.org/W2115678156","https://openalex.org/W2115767337","https://openalex.org/W2115939443","https://openalex.org/W2117201299","https://openalex.org/W2117273677","https://openalex.org/W2117753040","https://openalex.org/W2118246164","https://openalex.org/W2118526609","https://openalex.org/W2119276480","https://openalex.org/W2120512069","https://openalex.org/W2121531763","https://openalex.org/W2122068661","https://openalex.org/W2122421045","https://openalex.org/W2122438081","https://openalex.org/W2123370218","https://openalex.org/W2124649685","https://openalex.org/W2126466006","https://openalex.org/W2126816036","https://openalex.org/W2127230663","https://openalex.org/W2127469308","https://openalex.org/W2128114769","https://openalex.org/W2128227827","https://openalex.org/W2129461125","https://openalex.org/W2129907266","https://openalex.org/W2130001957","https://openalex.org/W2130077037","https://openalex.org/W2130494035","https://openalex.org/W2130701492","https://openalex.org/W2131145551","https://openalex.org/W2131153078","https://openalex.org/W2131629478","https://openalex.org/W2132238722","https://openalex.org/W2132341951","https://openalex.org/W2133604043","https://openalex.org/W2133980123","https://openalex.org/W2134800079","https://openalex.org/W2135565251","https://openalex.org/W2136796229","https://openalex.org/W2137124858","https://openalex.org/W2138190969","https://openalex.org/W2138270253","https://openalex.org/W2139216037","https://openalex.org/W2140869203","https://openalex.org/W2141093691","https://openalex.org/W2141688036","https://openalex.org/W2141955571","https://openalex.org/W2142403957","https://openalex.org/W2143187571","https://openalex.org/W2144725782","https://openalex.org/W2145452684","https://openalex.org/W2145903120","https://openalex.org/W2146517120","https://openalex.org/W2147008415","https://openalex.org/W2147061117","https://openalex.org/W2147354890","https://openalex.org/W2149093225","https://openalex.org/W2149573313","https://openalex.org/W2149615023","https://openalex.org/W2149770991","https://openalex.org/W2150677022","https://openalex.org/W2150896151","https://openalex.org/W2151732271","https://openalex.org/W2152745352","https://openalex.org/W2154431984","https://openalex.org/W2155055077","https://openalex.org/W2156896172","https://openalex.org/W2158297569","https://openalex.org/W2159322582","https://openalex.org/W2159946869","https://openalex.org/W2160444236","https://openalex.org/W2161203530","https://openalex.org/W2161546116","https://openalex.org/W2162578061","https://openalex.org/W2163111452","https://openalex.org/W2164095252","https://openalex.org/W2164112721","https://openalex.org/W2164981820","https://openalex.org/W2165748644","https://openalex.org/W2166884865","https://openalex.org/W2167434988","https://openalex.org/W2168142506","https://openalex.org/W2168156874","https://openalex.org/W2168909179","https://openalex.org/W2169035256","https://openalex.org/W2169600089","https://openalex.org/W2171426009","https://openalex.org/W2171956120","https://openalex.org/W2315884304","https://openalex.org/W2493581502","https://openalex.org/W2964792145","https://openalex.org/W6948519597","https://openalex.org/W7096002534","https://openalex.org/W7098124190"],"related_works":["https://openalex.org/W112041751","https://openalex.org/W1975318085","https://openalex.org/W7096660144","https://openalex.org/W2258305572","https://openalex.org/W2969677523","https://openalex.org/W4402470635","https://openalex.org/W3002396101","https://openalex.org/W2945489451","https://openalex.org/W2418139410","https://openalex.org/W1992413107"],"abstract_inverted_index":{"Recent":[0],"proceedings":[1],"in":[2,9,31,57,69,86,96,143,156,165,240,262,332,371,381,417,454,492,508,519,526,550,581,667],"biotechnology":[3],"have":[4,253],"enabled":[5],"completely":[6],"new":[7],"avenues":[8],"life":[10,97,220],"science":[11],"research":[12,102,217],"to":[13,45,119,292,303,334,342,392,434,474,482,552,663],"be":[14,29,330,390,415,490,661,679],"explored.":[15],"By":[16,607,636],"allowing":[17],"increased":[18,583],"parallelization":[19],"an":[20,440,582],"ever-increasing":[21],"complexity":[22],"of":[23,77,93,112,151,194,227,270,274,324,345,354,367,409,456,469,486,501,522,540,556,603,614,669],"cell":[24,47,52,101,106,108,123,208,589,605],"samples":[25,318],"or":[26,79,571],"experiments":[27,126,487],"can":[28,61,414,489,660,678],"investigated":[30,597,627],"shorter":[32,387],"time":[33],"and":[34,110,118,145,149,161,172,190,197,237,299,319,384,402,461,476,510,536,567,628,639],"at":[35,49],"a":[36,139,166,289,378,424,450,592,675],"lower":[37],"cost.":[38],"This":[39,328],"facilitates":[40],"for":[41,147,178,186,206,258,305,364,430,437,538,647],"example":[42],"large-scale":[43],"efforts":[44],"study":[46,664],"heterogeneity":[48,124,590],"the":[50,87,94,173,219,234,268,272,281,293,335,350,355,407,445,484,499,523,527,546,554,576,610,615,649],"single":[51,132,195,604,645],"level,":[53],"by":[54,169,222,395,598],"analyzing":[55],"cells":[56,78,158,625,646],"parallel":[58,75,211,243,359,658],"that":[59,307,413,488,656],"also":[60,300,362,497,563,579],"include":[62],"global":[63],"genomic":[64],"analyses.":[65],"The":[66,182],"work":[67],"presented":[68],"this":[70,448],"thesis":[71],"focuses":[72],"on":[73,122,131,255,280,444,545,575,609,673],"massively":[74,242,657],"analysis":[76,193],"nucleic":[80],"acid":[81],"samples,":[82,561],"demonstrating":[83],"technology":[84,95],"developments":[85],"field":[88],"as":[89,91,105,278,399],"well":[90],"use":[92,301],"sciences.":[98],"In":[99,134,249,284,421,530,586],"stem":[100,152,157],"issues":[103],"such":[104,398],"morphology,":[107],"differentiation":[109],"effects":[111],"reprogramming":[113],"factors":[114],"are":[115,127,246,634],"frequently":[116],"studied,":[117],"obtain":[120],"information":[121],"these":[125,386,670],"preferably":[128],"carried":[129],"out":[130],"cells.":[133,153],"paper":[135,250,285,422,531,587],"I":[136],"we":[137,252,287,654],"used":[138,177,429,549,662],"high-density":[140],"microwell":[141],"device":[142],"silicon":[144],"glass":[146],"culturing":[148],"screening":[150,167],"Maintained":[154],"pluripotency":[155],"from":[159,229,559,644],"human":[160,593,616],"mouse":[162],"was":[163,175,428,459,548],"demonstrated":[164,279],"assay":[168],"antibody":[170],"staining":[171],"chip":[174,183],"furthermore":[176],"studying":[179],"neural":[180],"differentiation.":[181],"format":[184],"allows":[185],"low":[187],"sample":[188,260,296,346],"volumes":[189],"rapid":[191,290],"high-throughput":[192],"cells,":[196],"is":[198,214,361,377,479,595,626,652],"compatible":[199],"with":[200,267,320,439,462,542,569],"Fluorescence":[201],"Activated":[202],"Cell":[203],"Sorting":[204],"(FACS)":[205],"precise":[207],"selection.":[209],"Massively":[210,358],"DNA":[212,311,325,471],"sequencing":[213,231,244,265,326,356,360,366,383,419,494,528,602,640,659],"revolutionizing":[215],"genomics":[216],"throughout":[218],"sciences":[221],"constantly":[223],"producing":[224],"increasing":[225,271],"amounts":[226],"data":[228,276,352,557],"one":[230,493],"run.":[232,357,420],"However,":[233,373,504],"reagent":[235],"costs":[236],"labor":[238],"requirements":[239],"current":[241],"protocols":[245],"still":[247],"substantial.":[248],"II-IV":[251],"focused":[254],"flow-sorting":[256,302,537],"techniques":[257],"improved":[259],"preparation":[261],"bead-based":[263],"massive":[264],"platforms,":[266],"aim":[269],"amount":[273],"quality":[275,509],"output,":[277],"Roche/454":[282],"platform.":[283],"II":[286],"demonstrate":[288,655],"alternative":[291],"existing":[294],"shotgun":[295],"titration":[297],"protocol":[298],"enrich":[304,436],"beads":[306,438,541,568],"carry":[308],"amplified":[309,441,573],"template":[310],"after":[312],"emulsion":[313,477],"PCR,":[314],"thus":[315,348],"obtaining":[316],"pure":[317],"no":[321,463,570],"downstream":[322],"sacrifice":[323],"quality.":[327,585],"should":[329],"seen":[331],"comparison":[333],"standard":[336,396,480],"454-enrichment":[337],"protocol,":[338],"which":[339,516,648,674],"gives":[340],"rise":[341],"varying":[343],"degrees":[344],"purity,":[347],"affecting":[349],"sequence":[351,411,464,584],"output":[353],"useful":[363],"deep":[365],"specific":[368,534],"PCR-amplified":[369],"targets":[370],"parallel.":[372],"unspecific":[374],"product":[375,574],"formation":[376],"common":[379],"problem":[380],"amplicon":[382],"since":[385],"products":[388],"may":[389,517],"difficult":[391],"fully":[393],"remove":[394],"procedures":[397],"gel":[400],"purification,":[401],"their":[403],"presence":[404],"inevitably":[405],"reduces":[406],"number":[408,485],"target":[410,442],"reads":[412],"obtained":[416,460,558],"each":[418],"III":[423],"gene-specific":[425],"fluorescent":[426],"probe":[427],"target-specific":[431],"FACS":[432],"enrichment":[433],"specifically":[435],"gene":[443],"surface.":[446],"Through":[447],"procedure":[449,481],"nearly":[451],"three-fold":[452],"increase":[453],"fraction":[455],"informative":[457],"sequences":[458],"bias":[465],"introduced.":[466],"Barcode":[467],"labeling":[468,535],"different":[470,524,543,560,624],"libraries":[472,507,525],"prior":[473],"pooling":[475],"PCR":[478],"maximize":[483],"run":[491],"lane,":[495],"while":[496,562],"decreasing":[498],"impact":[500],"technical":[502],"noise.":[503],"variation":[505,666],"between":[506,623],"GC":[511],"content":[512],"affects":[513],"amplification":[514],"efficiency,":[515],"result":[518],"biased":[520],"fractions":[521],"data.":[529],"IV":[532],"barcode":[533],"normalization":[539],"barcodes":[544],"surface":[547],"order":[551],"weigh":[553],"proportion":[555],"removing":[564],"mixed":[565],"beads,":[566],"poorly":[572],"surface,":[577],"hence":[578],"resulting":[580],"V,":[588],"within":[591],"being":[594,596],"low-coverage":[599],"whole":[600],"genome":[601],"material.":[606],"focusing":[608],"most":[611],"variable":[612,630],"portion":[613],"genome,":[617],"polyguanine":[618,631,641],"nucleotide":[619,642],"repeat":[620,632],"regions,":[621],"variability":[622],"highly":[629],"loci":[633],"identified.":[635],"selectively":[637],"amplifying":[638],"repeats":[643],"phylogenetic":[650,676],"relationship":[651],"known,":[653],"cell-cell":[665],"length":[668],"repeats,":[671],"based":[672],"tree":[677],"drawn.":[680]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}