{"id":"https://openalex.org/W579084071","doi":null,"title":"Functional characterization of glucocorticoid receptor sequences required for steroid-induced cell death.","display_name":"Functional characterization of glucocorticoid receptor sequences required for steroid-induced cell death.","publication_year":1991,"publication_date":"1991-01-01","ids":{"openalex":"https://openalex.org/W579084071","mag":"579084071"},"language":"en","primary_location":{"id":"pmh:oai:repository.arizona.edu:10150/185651","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10150/185651","pdf_url":null,"source":{"id":"https://openalex.org/S4306400271","display_name":"UA Campus Repository (The University of Arizona)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I138006243","host_organization_name":"University of Arizona","host_organization_lineage":["https://openalex.org/I138006243"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation-Reproduction (electronic)"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079956465","display_name":"Ellen S. Dieken","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ellen S. Dieken","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5079956465"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10756","display_name":"Estrogen and related hormone effects","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10756","display_name":"Estrogen and related hormone effects","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glucocorticoid-receptor","display_name":"glucocorticoid receptor","score":0.9729999899864197},{"id":"https://openalex.org/keywords/glucocorticoid-resistance","display_name":"glucocorticoid resistance","score":0.8289999961853027},{"id":"https://openalex.org/keywords/s49-lymphoma-cells","display_name":"S49 lymphoma cells","score":0.7310000061988831},{"id":"https://openalex.org/keywords/n-terminal-domain","display_name":"N-terminal domain","score":0.6200000047683716},{"id":"https://openalex.org/keywords/transcriptional-activation","display_name":"transcriptional activation","score":0.703000009059906},{"id":"https://openalex.org/keywords/vp16","display_name":"VP16","score":0.47200000286102295},{"id":"https://openalex.org/keywords/herpes-simplex-virus-type-1","display_name":"herpes simplex virus type 1","score":0.6949999928474426},{"id":"https://openalex.org/keywords/lymphocyte-apoptosis","display_name":"lymphocyte apoptosis","score":0.7110000252723694},{"id":"https://openalex.org/keywords/steroid-hormone-action","display_name":"steroid hormone action","score":0.4339999854564667},{"id":"https://openalex.org/keywords/rna-splicing","display_name":"RNA splicing","score":0.453000009059906},{"id":"https://openalex.org/keywords/complementation-tests","display_name":"complementation assay","score":0.7149999737739563}],"concepts":[{"id":"https://openalex.org/C59493245","wikidata":"https://www.wikidata.org/wiki/Q14877410","display_name":"Glucocorticoid receptor","level":3,"score":0.6148281693458557},{"id":"https://openalex.org/C2780841215","wikidata":"https://www.wikidata.org/wiki/Q422549","display_name":"Glucocorticoid","level":2,"score":0.6104024052619934},{"id":"https://openalex.org/C2780902042","wikidata":"https://www.wikidata.org/wiki/Q177911","display_name":"Steroid","level":3,"score":0.5408144593238831},{"id":"https://openalex.org/C124170894","wikidata":"https://www.wikidata.org/wiki/Q14914591","display_name":"Nuclear receptor coactivator 2","level":5,"score":0.4519609212875366},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.4269619584083557},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3971380889415741},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38086801767349243},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3687201738357544},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.28860652446746826},{"id":"https://openalex.org/C63932345","wikidata":"https://www.wikidata.org/wiki/Q422500","display_name":"Nuclear receptor","level":4,"score":0.23004496097564697},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.19429990649223328},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18571043014526367},{"id":"https://openalex.org/C71315377","wikidata":"https://www.wikidata.org/wiki/Q11364","display_name":"Hormone","level":2,"score":0.1585196852684021},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.14415323734283447}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:repository.arizona.edu:10150/185651","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10150/185651","pdf_url":null,"source":{"id":"https://openalex.org/S4306400271","display_name":"UA Campus Repository (The University of Arizona)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I138006243","host_organization_name":"University of Arizona","host_organization_lineage":["https://openalex.org/I138006243"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation-Reproduction (electronic)"},{"id":"mag:579084071","doi":null,"is_oa":false,"landing_page_url":"https://arizona.openrepository.com/handle/10150/185651","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.46810001134872437}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":115,"referenced_works":["https://openalex.org/W7532581","https://openalex.org/W65199599","https://openalex.org/W143316405","https://openalex.org/W200955117","https://openalex.org/W1213045642","https://openalex.org/W1482755017","https://openalex.org/W1493507586","https://openalex.org/W1509433670","https://openalex.org/W1523542926","https://openalex.org/W1538933671","https://openalex.org/W1541971708","https://openalex.org/W1550589075","https://openalex.org/W1552240535","https://openalex.org/W1558227941","https://openalex.org/W1558812914","https://openalex.org/W1571259701","https://openalex.org/W1589415602","https://openalex.org/W1591168462","https://openalex.org/W1593007620","https://openalex.org/W1598811569","https://openalex.org/W1600085067","https://openalex.org/W1648720631","https://openalex.org/W1828569124","https://openalex.org/W1842900458","https://openalex.org/W1864683138","https://openalex.org/W1889314752","https://openalex.org/W1964461239","https://openalex.org/W1965268115","https://openalex.org/W1968713580","https://openalex.org/W1969331809","https://openalex.org/W1970038537","https://openalex.org/W1971960644","https://openalex.org/W1972364920","https://openalex.org/W1977347668","https://openalex.org/W1978799355","https://openalex.org/W1980722886","https://openalex.org/W1987189376","https://openalex.org/W1987557547","https://openalex.org/W1988432444","https://openalex.org/W1993125057","https://openalex.org/W1996251138","https://openalex.org/W1998809543","https://openalex.org/W1999198359","https://openalex.org/W1999745373","https://openalex.org/W2002910558","https://openalex.org/W2003054104","https://openalex.org/W2003600809","https://openalex.org/W2004376767","https://openalex.org/W2005914718","https://openalex.org/W2008317513","https://openalex.org/W2010234214","https://openalex.org/W2012324003","https://openalex.org/W2013567194","https://openalex.org/W2014107736","https://openalex.org/W2014435603","https://openalex.org/W2015617558","https://openalex.org/W2016683567","https://openalex.org/W2018796983","https://openalex.org/W2021956984","https://openalex.org/W2025267983","https://openalex.org/W2026891958","https://openalex.org/W2027216583","https://openalex.org/W2029475329","https://openalex.org/W2030315227","https://openalex.org/W2030666031","https://openalex.org/W2036931150","https://openalex.org/W2037653294","https://openalex.org/W2038217645","https://openalex.org/W2041781038","https://openalex.org/W2041807810","https://openalex.org/W2042607958","https://openalex.org/W2042818355","https://openalex.org/W2045128899","https://openalex.org/W2048427461","https://openalex.org/W2049425355","https://openalex.org/W2053700118","https://openalex.org/W2057257609","https://openalex.org/W2060484288","https://openalex.org/W2060903877","https://openalex.org/W2063110155","https://openalex.org/W2064345521","https://openalex.org/W2065679706","https://openalex.org/W2065867057","https://openalex.org/W2069179692","https://openalex.org/W2071525868","https://openalex.org/W2071961761","https://openalex.org/W2075310540","https://openalex.org/W2075507370","https://openalex.org/W2075788654","https://openalex.org/W2077993951","https://openalex.org/W2078038295","https://openalex.org/W2078667106","https://openalex.org/W2080214680","https://openalex.org/W2081085542","https://openalex.org/W2089126365","https://openalex.org/W2090112051","https://openalex.org/W2097228095","https://openalex.org/W2097483036","https://openalex.org/W2100837269","https://openalex.org/W2104360623","https://openalex.org/W2105014300","https://openalex.org/W2109133792","https://openalex.org/W2119725374","https://openalex.org/W2129972061","https://openalex.org/W2132781864","https://openalex.org/W2144634347","https://openalex.org/W2145981915","https://openalex.org/W2146346171","https://openalex.org/W2148021183","https://openalex.org/W2160277752","https://openalex.org/W2161628453","https://openalex.org/W2168847939","https://openalex.org/W2438965929","https://openalex.org/W2439919751","https://openalex.org/W2495416561"],"related_works":["https://openalex.org/W4248091690","https://openalex.org/W2233071446","https://openalex.org/W2130064105","https://openalex.org/W7097954153","https://openalex.org/W2090137967","https://openalex.org/W2014173733","https://openalex.org/W761731857","https://openalex.org/W148604542","https://openalex.org/W2147416879","https://openalex.org/W200955117"],"abstract_inverted_index":{"Glucocorticoid":[0],"induction":[1,296,370],"of":[2,20,36,56,115,151,159,166,192,216,234,270,287,297,371],"cell":[3,45,79,226],"death":[4],"(apoptosis)":[5],"in":[6,27,38,204,259,279,310],"mouse":[7,298],"lymphoma":[8],"S49":[9,43,78,246,366],"cells":[10,131,357],"has":[11,314,320],"long":[12],"been":[13,321],"studied":[14],"as":[15,112],"a":[16,113,138,210,224,276,315],"molecular":[17,66],"genetic":[18],"model":[19],"steroid":[21,40,101],"hormone":[22],"action.":[23],"The":[24,304],"research":[25],"described":[26],"this":[28,260,285],"dissertation":[29],"focuses":[30],"on":[31],"understanding":[32],"the":[33,57,65,75,85,145,157,168,182,190,193,201,251,272,288,311,341,351],"transcriptional":[34,206,369],"control":[35],"apoptosis":[37,247,367],"two":[39,76],"resistant":[41],"nt\u2071":[42,59,77,96,133,147,171,183,205],"mutant":[44,58,219],"lines":[46],"(S49.55r":[47],"and":[48,70,82,84,102,122,137,170,177,218,248,319],"S49.143r).":[49],"These":[50,262],"studies":[51],"extend":[52],"earlier":[53],"biochemical":[54],"analysis":[55,93],"glucocorticoid":[60,139],"receptor":[61,134,148,184,289],"(nt\u2071":[62],"GR)":[63],"to":[64,154,179,199,291,334],"level":[67,158],"by":[68],"isolating":[69],"characterizing":[71],"GR":[72,97,172,220,242,252,273,312,352],"cDNA":[73,221],"from":[74,125,331,340],"lines,":[80],"S49.55r":[81],"S49.143r,":[83],"wild":[86],"type":[87],"(wt)":[88],"parental":[89],"line,":[90],"S49.A2.":[91],"This":[92],"revealed":[94],"that":[95,144,189,241,250,266,355,365],"transcripts":[98],"encode":[99],"intact":[100],"DNA":[103],"binding":[104],"domains":[105],"but":[106],"lack":[107],"404":[108],"amino-terminal":[109],"(N-terminal)":[110],"residues":[111],"result":[114],"aberrant":[116],"RNA":[117],"splicing":[118],"between":[119],"exons":[120],"1":[121],"3.":[123],"Results":[124,330],"transient":[126],"co-transfection":[127],"experiments":[128,332],"into":[129,223],"CV1":[130],"using":[132],"expression":[135],"plasmids":[136],"responsive":[140],"reporter":[141],"gene":[142],"demonstrate":[143,354],"truncated":[146],"is":[149,197,243,254],"capable":[150],"inducing":[152],"transcription":[153],"only":[155],"10%":[156],"wt":[160,169,217],"GR.":[161],"Gene":[162],"fusions":[163,360],"containing":[164],"portions":[165],"both":[167],"coding":[173],"sequences":[174,339],"were":[175],"constructed":[176],"used":[178],"functionally":[180,306],"map":[181],"mutation.":[185],"It":[186],"was":[187,229,239],"found":[188],"loss":[191],"N-terminal":[194],"domain":[195,328],"alone":[196],"sufficient":[198],"cause":[200],"observed":[202],"defect":[203],"transactivation.":[207],"In":[208],"addition,":[209],"complementation":[211,258],"assay":[212],"utilizing":[213],"stable":[214],"transfection":[215],"constructs":[222],"GR-deficient":[225],"line":[227],"(7r)":[228],"developed.":[230],"By":[231],"measuring":[232],"steroid-sensitivity":[233],"various":[235],"7r":[236,356],"derivatives,":[237],"it":[238],"determined":[240],"rate-limiting":[244],"for":[245,257,294,350],"moreover,":[249],"N-terminus":[253,274,313,353],"absolutely":[255],"required":[256],"system.":[261],"data":[263],"also":[264],"indicate":[265],"at":[267],"physiological":[268],"levels":[269],"receptor,":[271],"plays":[275],"crucial":[277],"role":[278],"controlling":[280],"lymphocyte":[281],"apoptosis,":[282],"even":[283],"though":[284],"portion":[286],"seems":[290],"be":[292],"dispensable":[293],"low-level":[295],"mammary":[299],"tumor":[300],"virus":[301],"(MMTV)":[302],"transcription.":[303],"smallest":[305],"defined":[307],"transactivation":[308],"region":[309],"net":[316],"negative":[317],"charge":[318],"named":[322],"enh2,":[323],"tau1":[324],"or":[325],"acidic":[326],"activation":[327],"(AAD).":[329],"designed":[333],"test":[335],"whether":[336],"similar":[337],"activating":[338],"herpes":[342],"simplex":[343],"virus-1":[344],"(HSV-1)":[345],"VP16":[346],"protein":[347],"can":[348],"substitute":[349],"expressing":[358],"VP-GR":[359],"are":[361],"indeed":[362],"steroid-sensitive,":[363],"suggesting":[364],"requires":[368],"specific":[372],"genes.":[373]},"counts_by_year":[],"updated_date":"2026-10-01T08:54:57.152042","created_date":"2016-06-24T00:00:00"}