{"id":"https://openalex.org/W570583199","doi":null,"title":"Physiological response of the methylotrophic yeast Pichia pastoris to recombinant protein expression","display_name":"Physiological response of the methylotrophic yeast Pichia pastoris to recombinant protein expression","publication_year":2004,"publication_date":"2004-01-01","ids":{"openalex":"https://openalex.org/W570583199","mag":"570583199"},"language":"en","primary_location":{"id":"pmh:oai:digitalcommons.unl.edu:dissertations-10372","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.unl.edu/dissertations/AAI3159558","pdf_url":null,"source":{"id":"https://openalex.org/S4306400577","display_name":"Lincoln (University of Nebraska)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114395901","host_organization_name":"University of Nebraska\u2013Lincoln","host_organization_lineage":["https://openalex.org/I114395901"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ETD collection for University of Nebraska-Lincoln","raw_type":"text"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044333313","display_name":"Bradley A. Plantz","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Bradley A. Plantz","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5044333313"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/komagataella-phaffii","display_name":"Pichia pastoris","score":1.0},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9769999980926514},{"id":"https://openalex.org/keywords/interferon-tau","display_name":"interferon-tau","score":0.7670000195503235},{"id":"https://openalex.org/keywords/metabolic-burden","display_name":"metabolic burden","score":0.6230000257492065},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.6729999780654907},{"id":"https://openalex.org/keywords/decoupling-point","display_name":"decoupling point","score":0.7160000205039978},{"id":"https://openalex.org/keywords/protease-degradation","display_name":"protease degradation","score":0.8149999976158142},{"id":"https://openalex.org/keywords/metalloproteinases","display_name":"metalloproteinases","score":0.8460000157356262},{"id":"https://openalex.org/keywords/aspartic-protease","display_name":"aspartic protease","score":0.9229999780654907},{"id":"https://openalex.org/keywords/serine-protease","display_name":"serine protease","score":0.9679999947547913},{"id":"https://openalex.org/keywords/adenylate-energy-charge","display_name":"energy charge","score":0.878000020980835},{"id":"https://openalex.org/keywords/energy-balance","display_name":"energy balance","score":0.7689999938011169},{"id":"https://openalex.org/keywords/beta-galactosidase","display_name":"beta-galactosidase","score":0.7509999871253967},{"id":"https://openalex.org/keywords/cytokine-production","display_name":"cytokine production","score":0.460999995470047}],"concepts":[{"id":"https://openalex.org/C2781372317","wikidata":"https://www.wikidata.org/wiki/Q148697","display_name":"Pichia pastoris","level":4,"score":0.9752276539802551},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.7991340160369873},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.739530086517334},{"id":"https://openalex.org/C2779703480","wikidata":"https://www.wikidata.org/wiki/Q125153","display_name":"Pichia","level":5,"score":0.6180526614189148},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4435684084892273},{"id":"https://openalex.org/C6856738","wikidata":"https://www.wikidata.org/wiki/Q331672","display_name":"Protein expression","level":3,"score":0.4151095449924469},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38211917877197266},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3684998154640198},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1760563850402832}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:digitalcommons.unl.edu:dissertations-10372","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.unl.edu/dissertations/AAI3159558","pdf_url":null,"source":{"id":"https://openalex.org/S4306400577","display_name":"Lincoln (University of Nebraska)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114395901","host_organization_name":"University of Nebraska\u2013Lincoln","host_organization_lineage":["https://openalex.org/I114395901"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ETD collection for University of Nebraska-Lincoln","raw_type":"text"},{"id":"mag:570583199","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.unl.edu/cgi/viewcontent.cgi?article=10372&context=dissertations","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2171213278","https://openalex.org/W7080972064","https://openalex.org/W2131510511","https://openalex.org/W7080968845","https://openalex.org/W7099102474","https://openalex.org/W2094302069","https://openalex.org/W7098987139","https://openalex.org/W2414668847","https://openalex.org/W3201597320","https://openalex.org/W153546705"],"abstract_inverted_index":{"The":[0,65,99,215,231,258],"production":[1,52,96,139,227],"of":[2,46,67,84,108,140,150,208,223,228,281,381],"recombinant":[3,229],"proteins":[4],"for":[5,94,236,243,254,268],"use":[6],"as":[7,24,303],"human":[8],"or":[9,241,251],"animal":[10],"therapeutic":[11],"purposes":[12],"requires":[13],"a":[14,56,75,111,121,129,179,346,374],"manufacturing":[15],"process":[16,57,80,124],"that":[17,58,136,147,190,201,287],"is":[18],"both":[19],"in":[20,38,55,61,205,299,357,377],"control":[21,62],"and":[22,30,77,88,97,173,193,253,273,351,371],"rugged":[23],"mandated":[25],"by":[26],"the":[27,35,39,44,68,85,91,106,138,148,157,163,170,174,212,220,224,269,278,300,309,316,331,336,358,364,378,382],"US":[28,86],"Food":[29,87],"Drug":[31,89],"Administration.":[32],"When":[33,307,328],"expressing":[34,256,329],"cytokine":[36],"roIFN-\u03c4":[37,109,199],"methylotrophic":[40],"yeast":[41],"Pichia":[42],"pastoris":[43,289],"yield":[45,149,332],"product":[47,141,151,177],"was":[48,59,72,125,142,155,185,263,290,373],"highly":[49],"variable":[50],"between":[51],"runs":[53],"resulting":[54],"neither":[60],"nor":[63],"rugged.":[64],"purpose":[66],"work":[69,104,189],"presented":[70],"herein":[71],"to":[73,265,275,293,312,325,343,363,369],"develop":[74],"stable":[76,122],"predictable":[78],"fermentation":[79,123,134],"which":[81],"meets":[82],"requirements":[83],"Administration,":[90],"governing":[92],"body":[93],"biologic":[95],"distribution.":[98],"central":[100],"hypothesis":[101,120,217],"guiding":[102],"this":[103,119,188],"was:":[105,219],"expression":[107],"places":[110],"metabolic":[112],"burden":[113],"on":[114,239,248],"P.":[115,288],"pastoris.":[116],"To":[117],"test":[118],"developed":[126],"based":[127],"upon":[128],"quasi-steady":[130],"state":[131],"fed":[132],"batch":[133],"method":[135],"assumed":[137],"growth":[143,247,271,279,350],"associated.":[144],"We":[145],"observed":[146],"dropped":[152,311],"after":[153,169,211,246,339],"what":[154],"coined":[156],"decoupling":[158,171,213],"point.":[159,214],"Therefore,":[160],"we":[161],"tested":[162,218],"working":[164],"hypothesis:":[165],"protease":[166],"levels":[167],"increase":[168,204],"point":[172],"enzymes":[175],"degrade":[176],"at":[178],"greater":[180],"rate":[181,207],"than":[182],"synthesis.":[183],"It":[184],"determined":[186],"from":[187,341,366],"metallo,":[191],"aspartyl":[192],"serinyl":[194],"proteases":[195],"are":[196,322],"active":[197],"against":[198],"but":[200,315],"no":[202],"significant":[203],"their":[206],"synthesis":[209],"occurs":[210],"final":[216],"energy":[221,260,297],"balance":[222],"cell":[225],"regulates":[226],"proteins.":[230],"adenylate":[232,259,296,383],"pools":[233],"were":[234],"measured":[235],"cultures":[237,244,255],"growing":[238],"glycerol":[240,250,342],"methanol,":[242,252,344],"starved":[245],"either":[249],"\u03b2-galactosidase.":[257],"charge":[261,298],"(AEC)":[262],"found":[264,274],"be":[266],"different":[267],"two":[270],"conditions":[272],"vary":[276],"over":[277],"curve":[280],"each":[282],"culture.":[283],"These":[284,320],"results":[285,321],"show":[286],"not":[291],"able":[292],"sustain":[294],"it's":[295],"same":[301],"manner":[302],"Saccharomyces":[304],"cerevisiae":[305],".":[306],"starving,":[308],"AEC":[310,359],"near":[313],"0.15":[314],"cells":[317],"remained":[318],"viable.":[319],"very":[323],"similar":[324],"S.":[326],"cerevisiae.":[327],"\u03b2-galactosidase":[330,367],"increased":[333],"sharply":[334],"during":[335,348],"lag":[337],"phase":[338],"transition":[340],"reached":[345],"peak":[347],"mid-log":[349],"then":[352],"declined.":[353],"A":[354],"small":[355],"drop":[356,376],"occurred":[360],"just":[361],"prior":[362],"shift":[365],"accumulation":[368],"loss,":[370],"there":[372],"concomitant":[375],"total":[379],"size":[380],"pool.":[384]},"counts_by_year":[],"updated_date":"2026-08-26T07:47:46.906454","created_date":"2016-06-24T00:00:00"}