{"id":"https://openalex.org/W569752124","doi":"https://doi.org/10.1016/j.jchromb.2015.06.003","title":"Development of an aptamer-affinity chromatography for efficient single step purification of Concanavalin A from Canavalia ensiformis","display_name":"Development of an aptamer-affinity chromatography for efficient single step purification of Concanavalin A from Canavalia ensiformis","publication_year":2015,"publication_date":"2015-06-12","ids":{"openalex":"https://openalex.org/W569752124","doi":"https://doi.org/10.1016/j.jchromb.2015.06.003","mag":"569752124","pmid":"https://pubmed.ncbi.nlm.nih.gov/26102634"},"language":"en","primary_location":{"id":"doi:10.1016/j.jchromb.2015.06.003","doi":"https://doi.org/10.1016/j.jchromb.2015.06.003","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jchromb.2015.06.003","pdf_url":null,"source":{"id":"https://openalex.org/S103248676","display_name":"Journal of Chromatography B","issn_l":"1570-0232","issn":["1570-0232","1873-376X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chromatography B","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009297315","display_name":"Rajesh Ahirwar","orcid":"https://orcid.org/0000-0002-3406-7436","observed_orcids":["https://orcid.org/0000-0002-3406-7436"]},"institutions":[{"id":"https://openalex.org/I177828482","display_name":"Institute of Genomics and Integrative Biology","ror":"https://ror.org/05ef28661","country_code":"IN","type":"facility","lineage":["https://openalex.org/I177828482","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I66760702","display_name":"Council of Scientific and Industrial Research","ror":"https://ror.org/021wm7p51","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Rajesh Ahirwar","raw_affiliation_strings":["CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India; Academy of Scientific and Innovative Research, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India","Academy of Scientific and Innovative Research, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India","CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India; Academy of Scientific and Innovative Research, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India","institution_ids":["https://openalex.org/I177828482","https://openalex.org/I66760702"]},{"raw_affiliation_string":"Academy of Scientific and Innovative Research, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India","institution_ids":["https://openalex.org/I177828482"]},{"raw_affiliation_string":"CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India","institution_ids":["https://openalex.org/I177828482"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112305301","display_name":"Pradip Nahar","orcid":"https://orcid.org/0000-0003-3027-9455","observed_orcids":["https://orcid.org/0000-0003-3027-9455"]},"institutions":[{"id":"https://openalex.org/I177828482","display_name":"Institute of Genomics and Integrative Biology","ror":"https://ror.org/05ef28661","country_code":"IN","type":"facility","lineage":["https://openalex.org/I177828482","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I66760702","display_name":"Council of Scientific and Industrial Research","ror":"https://ror.org/021wm7p51","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Pradip Nahar","raw_affiliation_strings":["CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India; Academy of Scientific and Innovative Research, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India. Electronic address: pnahar@igib.res.in","Academy of Scientific and Innovative Research, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India","CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India; Academy of Scientific and Innovative Research, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India. Electronic address: pnahar@igib.res.in","institution_ids":["https://openalex.org/I177828482","https://openalex.org/I66760702"]},{"raw_affiliation_string":"Academy of Scientific and Innovative Research, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India","institution_ids":["https://openalex.org/I177828482"]},{"raw_affiliation_string":"CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110 007, India","institution_ids":["https://openalex.org/I177828482"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5112305301"],"corresponding_institution_ids":["https://openalex.org/I177828482","https://openalex.org/I66760702"],"apc_list":{"value":3640,"currency":"USD","value_usd":3640},"apc_paid":null,"fwci":1.694,"has_fulltext":false,"cited_by_count":33,"citation_normalized_percentile":{"value":0.83793848,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"997","issue":null,"first_page":"105","last_page":"109"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9458000063896179,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9458000063896179,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13128","display_name":"Chromatography in Natural Products","score":0.008899999782443047,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.007000000216066837,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/concanavalin-a","display_name":"concanavalin A","score":0.9300000071525574},{"id":"https://openalex.org/keywords/dna-aptamers","display_name":"DNA aptamers","score":0.8159999847412109},{"id":"https://openalex.org/keywords/canavalia-ensiformis","display_name":"Canavalia ensiformis","score":0.8479999899864197},{"id":"https://openalex.org/keywords/agarose-beads","display_name":"agarose beads","score":0.6800000071525574},{"id":"https://openalex.org/keywords/column-reusability","display_name":"column reusability","score":0.8619999885559082},{"id":"https://openalex.org/keywords/glycoprotein-isolation","display_name":"glycoprotein isolation","score":0.7459999918937683}],"concepts":[{"id":"https://openalex.org/C128972844","wikidata":"https://www.wikidata.org/wiki/Q418722","display_name":"Aptamer","level":2,"score":0.9242300987243652},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.8875687122344971},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8520342111587524},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.8313033580780029},{"id":"https://openalex.org/C2776490341","wikidata":"https://www.wikidata.org/wiki/Q1677262","display_name":"Canavalia ensiformis","level":2,"score":0.7003110647201538},{"id":"https://openalex.org/C2778476535","wikidata":"https://www.wikidata.org/wiki/Q390697","display_name":"Agarose","level":2,"score":0.6627393364906311},{"id":"https://openalex.org/C19869320","wikidata":"https://www.wikidata.org/wiki/Q419277","display_name":"Concanavalin A","level":3,"score":0.6348506212234497},{"id":"https://openalex.org/C40875361","wikidata":"https://www.wikidata.org/wiki/Q2356542","display_name":"Reagent","level":2,"score":0.4559205174446106},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14900371432304382},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.07127141952514648},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.05987417697906494},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0578514039516449},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D003208","descriptor_name":"Concanavalin A","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D003208","descriptor_name":"Concanavalin A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003208","descriptor_name":"Concanavalin A","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012965","descriptor_name":"Sodium Chloride","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D032449","descriptor_name":"Canavalia","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.jchromb.2015.06.003","doi":"https://doi.org/10.1016/j.jchromb.2015.06.003","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jchromb.2015.06.003","pdf_url":null,"source":{"id":"https://openalex.org/S103248676","display_name":"Journal of Chromatography B","issn_l":"1570-0232","issn":["1570-0232","1873-376X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chromatography B","raw_type":"journal-article"},{"id":"pmid:26102634","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26102634","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320721","display_name":"Council of Scientific and Industrial Research, India","ror":"https://ror.org/021wm7p51"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W428785392","https://openalex.org/W1579019422","https://openalex.org/W1903005968","https://openalex.org/W1976259945","https://openalex.org/W2029220534","https://openalex.org/W2069356720","https://openalex.org/W2073058586","https://openalex.org/W2076388440","https://openalex.org/W2078196016","https://openalex.org/W2089432463","https://openalex.org/W2100181290","https://openalex.org/W2124395350","https://openalex.org/W2133282256","https://openalex.org/W2154604015","https://openalex.org/W2169750556","https://openalex.org/W2170679909","https://openalex.org/W2299098059","https://openalex.org/W2806718487","https://openalex.org/W4298162434","https://openalex.org/W4317640874","https://openalex.org/W6697553227"],"related_works":["https://openalex.org/W2078196016","https://openalex.org/W2599979587","https://openalex.org/W4317640874","https://openalex.org/W106949509","https://openalex.org/W2038378843","https://openalex.org/W2069356720","https://openalex.org/W2018818367","https://openalex.org/W1559682908","https://openalex.org/W7204904231","https://openalex.org/W1978823642"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":4},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":7},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}