{"id":"https://openalex.org/W56690807","doi":null,"title":"Using error prone PCR in directed evolution to selected novel antibiotic resistances","display_name":"Using error prone PCR in directed evolution to selected novel antibiotic resistances","publication_year":2014,"publication_date":"2014-02-07","ids":{"openalex":"https://openalex.org/W56690807","mag":"56690807"},"language":"en","primary_location":{"id":"pmh:oai:wiredspace.wits.ac.za:10539/13691","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net10539/13691","pdf_url":null,"source":{"id":"https://openalex.org/S4306400603","display_name":"University of the Witwatersrand, Johannesburg Institutional Repository on DSpace (University of the Witwatersrand, Johannesburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I192619145","host_organization_name":"University of the Witwatersrand","host_organization_lineage":["https://openalex.org/I192619145"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057151779","display_name":"Phokela Apollonarius Comet Mogashoa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Phokela Apollonarius Comet Mogashoa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5057151779"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.8877000212669373,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.8877000212669373,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.03229999914765358,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.02979999966919422,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/error-prone-pcr","display_name":"error-prone PCR","score":0.8619999885559082},{"id":"https://openalex.org/keywords/directed-evolution","display_name":"directed evolution","score":0.9539999961853027},{"id":"https://openalex.org/keywords/antibiotic-resistance","display_name":"antibiotic resistance","score":0.9179999828338623},{"id":"https://openalex.org/keywords/rifampicin-resistance","display_name":"rifampicin resistance","score":0.8740000128746033},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9700000286102295},{"id":"https://openalex.org/keywords/minimum-inhibitory-concentration","display_name":"minimum inhibitory concentration","score":0.9190000295639038},{"id":"https://openalex.org/keywords/random-mutagenesis","display_name":"random mutagenesis","score":0.7120000123977661},{"id":"https://openalex.org/keywords/mobile-genetic-elements","display_name":"mobile genetic elements","score":0.6439999938011169},{"id":"https://openalex.org/keywords/transposable-elements","display_name":"transposable elements","score":0.8330000042915344},{"id":"https://openalex.org/keywords/plasmids","display_name":"plasmids","score":0.9850000143051147}],"concepts":[{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.45487555861473083},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.39909282326698303},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3785741925239563},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3622891902923584},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.33803316950798035}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:wiredspace.wits.ac.za:10539/13691","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net10539/13691","pdf_url":null,"source":{"id":"https://openalex.org/S4306400603","display_name":"University of the Witwatersrand, Johannesburg Institutional Repository on DSpace (University of the Witwatersrand, Johannesburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I192619145","host_organization_name":"University of the Witwatersrand","host_organization_lineage":["https://openalex.org/I192619145"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:56690807","doi":null,"is_oa":false,"landing_page_url":"https://wiredspace.wits.ac.za/bitstream/handle/10539/13691/MOGASHOA%20MSC.pdf;sequence=1","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5683000087738037,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W1500910904","https://openalex.org/W1545162664","https://openalex.org/W1928192356","https://openalex.org/W1969441047","https://openalex.org/W1998495168","https://openalex.org/W2005237155","https://openalex.org/W2011363330","https://openalex.org/W2011626631","https://openalex.org/W2026701298","https://openalex.org/W2044550428","https://openalex.org/W2058058143","https://openalex.org/W2076279677","https://openalex.org/W2080397181","https://openalex.org/W2081660660","https://openalex.org/W2082734817","https://openalex.org/W2108638835","https://openalex.org/W2109692334","https://openalex.org/W2118363342","https://openalex.org/W2128692282","https://openalex.org/W2129000413","https://openalex.org/W2129493063","https://openalex.org/W2131429608","https://openalex.org/W2135636849","https://openalex.org/W2142325518","https://openalex.org/W2144857212","https://openalex.org/W2144889532","https://openalex.org/W2149353210","https://openalex.org/W2158658436","https://openalex.org/W2159469354","https://openalex.org/W2162151303","https://openalex.org/W2166662833","https://openalex.org/W2170111718","https://openalex.org/W2316159096"],"related_works":["https://openalex.org/W7017290720","https://openalex.org/W3007364750","https://openalex.org/W2743143684","https://openalex.org/W2041075524","https://openalex.org/W2807173586","https://openalex.org/W4281774081","https://openalex.org/W2212271228","https://openalex.org/W2103385286","https://openalex.org/W1997437467","https://openalex.org/W7097057044"],"abstract_inverted_index":{"The":[0,118],"evolution":[1],"of":[2,12,20,48,64,73,79,160,173],"antibiotic":[3,21,91,176],"resistance":[4,22,92,112,161],"presents":[5],"an":[6,105,132,139],"escalating":[7],"problem":[8],"in":[9,104,115,131,171],"the":[10,18,71,148,174],"treatment":[11],"various":[13],"infectious":[14],"diseases":[15],"worldwide.":[16],"Although":[17],"origin":[19],"genes":[23,66],"is":[24,78],"not":[25],"generally":[26],"clearly":[27],"documented,":[28],"it":[29],"has":[30],"been":[31],"thought":[32],"that":[33],"they":[34],"evolved":[35],"from":[36,121],"specific":[37],"genetic":[38],"elements":[39],"which":[40,108],"eventually":[41],"managed":[42],"to":[43,45,100,113,134,147],"spread":[44],"other":[46,175],"microorganism":[47],"different":[49,126],"strains":[50],"and":[51,58,67,76,87],"species":[52],"through":[53],"mobile":[54],"genetics":[55],"elements,":[56],"transposons":[57],"plasmids.":[59],"Extensively":[60],"studying":[61],"all":[62],"aspects":[63],"these":[65],"their":[68],"impact":[69],"on":[70,85,125],"development":[72],"new":[74],"treatments":[75],"drugs":[77],"extreme":[80],"importance.":[81],"This":[82],"study":[83],"focuses":[84],"evolving":[86],"understanding":[88],"how":[89],"novel":[90],"develops.":[93],"Error":[94],"prone":[95],"PCR":[96],"(EP-PCR)":[97],"was":[98],"used":[99],"introduce":[101],"random":[102],"mutation":[103],"arr":[106],"gene":[107],"confers":[109],"high":[110],"level":[111],"rifampicin":[114,163],"E.":[116],"coli.":[117],"clones":[119,156],"obtained":[120],"EP-PCR":[122],"were":[123],"screened":[124],"antibiotics":[127],"with":[128,138],"varying":[129],"concentration":[130,143],"attempt":[133],"isolate":[135],"a":[136],"clone":[137],"increased":[140],"minimum":[141],"inhibitory":[142],"(MIC)":[144],"as":[145],"compared":[146],"wild":[149],"type":[150],"parent":[151],"strain":[152],"(pBstN49).":[153],"\\n":[154],"\\nSeveral":[155],"showed":[157,167],"decreased":[158],"levels":[159],"against":[162],"but":[164],"however":[165],"none":[166],"any":[168,172],"significant":[169],"increase":[170],"MICs":[177],"tested.":[178]},"counts_by_year":[],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2016-06-24T00:00:00"}