{"id":"https://openalex.org/W55772983","doi":null,"title":"[Effects of oxidative stress on barrier function of human retina pigment epithelium and its molecular mechanisms].","display_name":"[Effects of oxidative stress on barrier function of human retina pigment epithelium and its molecular mechanisms].","publication_year":2012,"publication_date":"2012-05-01","ids":{"openalex":"https://openalex.org/W55772983","mag":"55772983","pmid":"https://pubmed.ncbi.nlm.nih.gov/22932331"},"language":"zh","primary_location":{"id":"pmid:22932331","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22932331","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"[Zhonghua yan ke za zhi] Chinese journal of ophthalmology","raw_type":"English Abstract"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101434324","display_name":"Ling Bai","orcid":"https://orcid.org/0000-0003-3685-5548","observed_orcids":["https://orcid.org/0000-0003-3685-5548"]},"institutions":[{"id":"https://openalex.org/I4210131893","display_name":"Second Affiliated Hospital of Xi'an Jiaotong University","ror":"https://ror.org/03aq7kf18","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210131893"]},{"id":"https://openalex.org/I87445476","display_name":"Xi'an Jiaotong University","ror":"https://ror.org/017zhmm22","country_code":"CN","type":"education","lineage":["https://openalex.org/I87445476"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ling Bai","raw_affiliation_strings":["Department of Ophthalmology, the Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China. larkling@163.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ophthalmology, the Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710004, China. larkling@163.com","institution_ids":["https://openalex.org/I4210131893","https://openalex.org/I87445476"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079526895","display_name":"Huanhuan Yan","orcid":"https://orcid.org/0000-0002-0095-6307","observed_orcids":["https://orcid.org/0000-0002-0095-6307"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Huan-huan Yan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066599376","display_name":"De-xiu Zhang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"De-xiu Zhang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035598952","display_name":"Jianming Wang","orcid":"https://orcid.org/0000-0003-4118-6289","observed_orcids":["https://orcid.org/0000-0003-4118-6289"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jian-ming Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5109693362","display_name":"Nai-xue Sun","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nai-xue Sun","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.5646,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.67673122,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"48","issue":"5","first_page":"417","last_page":"22"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10170","display_name":"Retinal Diseases and Treatments","score":0.9398999810218811,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10170","display_name":"Retinal Diseases and Treatments","score":0.9398999810218811,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11872","display_name":"Barrier Structure and Function Studies","score":0.04659999907016754,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.9390000104904175},{"id":"https://openalex.org/keywords/hydrogen-peroxide","display_name":"hydrogen peroxide","score":0.9710000157356262},{"id":"https://openalex.org/keywords/retinal-pigment-epithelium","display_name":"retinal pigment epithelium","score":0.9890000224113464},{"id":"https://openalex.org/keywords/tight-junction-proteins","display_name":"tight junction proteins","score":0.8009999990463257},{"id":"https://openalex.org/keywords/barrier-function","display_name":"barrier function","score":0.6710000038146973},{"id":"https://openalex.org/keywords/transepithelial-electrical-resistance","display_name":"transepithelial electrical resistance","score":0.9490000009536743},{"id":"https://openalex.org/keywords/occludin","display_name":"occludin","score":0.8289999961853027},{"id":"https://openalex.org/keywords/claudins","display_name":"claudins","score":0.9350000023841858},{"id":"https://openalex.org/keywords/cell-viability","display_name":"cell viability","score":0.5899999737739563},{"id":"https://openalex.org/keywords/macular-edema","display_name":"macular edema","score":0.6710000038146973},{"id":"https://openalex.org/keywords/macular-hole","display_name":"macular hole","score":0.47699999809265137},{"id":"https://openalex.org/keywords/macular-degeneration","display_name":"macular degeneration","score":0.5889999866485596},{"id":"https://openalex.org/keywords/retinal-detachment","display_name":"retinal detachment","score":0.4449999928474426},{"id":"https://openalex.org/keywords/retinal-ischemia","display_name":"retinal ischemia","score":0.3569999933242798}],"concepts":[{"id":"https://openalex.org/C2775962179","wikidata":"https://www.wikidata.org/wiki/Q2013337","display_name":"Occludin","level":3,"score":0.6987612247467041},{"id":"https://openalex.org/C2778024200","wikidata":"https://www.wikidata.org/wiki/Q2346242","display_name":"Retinal pigment epithelium","level":3,"score":0.6771824359893799},{"id":"https://openalex.org/C51911345","wikidata":"https://www.wikidata.org/wiki/Q4863604","display_name":"Barrier function","level":2,"score":0.6662718057632446},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.5983080863952637},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.5968680381774902},{"id":"https://openalex.org/C2776151105","wikidata":"https://www.wikidata.org/wiki/Q898814","display_name":"Oxidative stress","level":2,"score":0.585518479347229},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5450270175933838},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.5339027643203735},{"id":"https://openalex.org/C177779419","wikidata":"https://www.wikidata.org/wiki/Q1053832","display_name":"Tight junction","level":2,"score":0.516312301158905},{"id":"https://openalex.org/C2780827179","wikidata":"https://www.wikidata.org/wiki/Q422001","display_name":"Retinal","level":2,"score":0.4853740930557251},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.43538758158683777},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.43226301670074463},{"id":"https://openalex.org/C529295009","wikidata":"https://www.wikidata.org/wiki/Q41301","display_name":"Epithelium","level":2,"score":0.4232605993747711},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4064604640007019},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.30626583099365234},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2677588164806366},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.22346526384353638},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.20929491519927979},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.16202884912490845},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002463","descriptor_name":"Cell Membrane Permeability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006861","descriptor_name":"Hydrogen Peroxide","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018384","descriptor_name":"Oxidative Stress","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019108","descriptor_name":"Tight Junctions","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D055213","descriptor_name":"Retinal Pigment Epithelium","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D055213","descriptor_name":"Retinal Pigment Epithelium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D055213","descriptor_name":"Retinal Pigment Epithelium","qualifier_ui":"Q000503","qualifier_name":"physiopathology","is_major_topic":true},{"descriptor_ui":"D062445","descriptor_name":"Claudin-1","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D062446","descriptor_name":"Claudin-2","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D062465","descriptor_name":"Claudin-3","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D062506","descriptor_name":"Claudin-4","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D062793","descriptor_name":"Occludin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:22932331","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22932331","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"[Zhonghua yan ke za zhi] Chinese journal of ophthalmology","raw_type":"English Abstract"},{"id":"mag:55772983","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22932331","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3030361868","https://openalex.org/W2410253399","https://openalex.org/W2382965564","https://openalex.org/W2092980812","https://openalex.org/W2169950408","https://openalex.org/W3032054322","https://openalex.org/W2385974182","https://openalex.org/W4384616272","https://openalex.org/W1975369096","https://openalex.org/W2004554703"],"abstract_inverted_index":{"OBJECTIVE:":[0],"To":[1],"investigate":[2],"the":[3,10,103,156,180,186,205,260,348,443,491,503,513],"effects":[4],"of":[5,15,51,56,69,81,92,102,144,147,161,194,198,214,263,277,329,350,445,494,507],"hydrogen":[6],"peroxide":[7],"(H2O2)":[8],"on":[9,53,502],"barrier":[11],"function":[12],"and":[13,37,105,116,120,132,150,170,216,331,336,455],"expression":[14,328,349,444,506],"tight":[16,514],"junction":[17],"protein":[18,332],"in":[19,185,333,341,490,497,505],"human":[20,30],"retinal":[21],"pigment":[22],"epithelium":[23],"(RPE)":[24],"cells.":[25],"METHODS:":[26],"Experimental":[27],"study.":[28,158],"The":[29,49,90,100,159,327],"RPE":[31,57,83,93,495],"cell":[32,54,145],"line":[33],"(D407)":[34],"were":[35,109,123,272,338],"cultured":[36],"treated":[38,41,65,131,266,344,453],"with":[39,66,207,267,345,454,512],"(H2O2":[40],"group)":[42],"or":[43],"without":[44,279,456],"H2O2":[45,52,70,130,137,268,280,457],"(normal":[46],"control":[47,134,181],"group).":[48],"effect":[50,210],"viability":[55,146],"cells":[58,84,94,163,199,264,278,343,452,496],"was":[59,85,98,153,188,217,352],"determined":[60,110],"by":[61,87,111],"MTT":[62],"test.":[63],"After":[64],"low":[67],"concentration":[68,152],"for":[71,155,174,200,269],"24":[72,212,270],"h":[73],"to":[74,95,107,125,179,219],"72":[75,220,237],"h,":[76],"transepithelial":[77],"electrical":[78],"resistance":[79],"(TER)":[80],"confluent":[82],"measured":[86],"epithelial":[88],"voltmeter.":[89],"permeability":[91,261,493],"sodium":[96,258],"fluorescein":[97],"measured.":[99],"expressions":[101],"occludin":[104],"claudin-1":[106,369],"-4":[108,337],"real-time":[112],"polymerase":[113],"chain":[114],"reaction":[115],"Western":[117],"blot":[118],"analysis.t-text":[119],"one-way":[121],"ANOVA":[122],"used":[124],"assess":[126],"statistical":[127],"significance":[128],"between":[129,451],"normal":[133],"groups.":[135],"RESULTS:":[136],"at":[138,167],"0.2":[139],"mmol/L":[140],"showed":[141],"no":[142],"decrease":[143],"D407":[148,162,342],"cells,":[149],"this":[151],"selected":[154],"present":[157],"TER":[160,187],"gradually":[164],"increased,":[165],"peaking":[166],"day":[168],"8":[169],"then":[171],"remained":[172],"stable":[173],"1":[175],"week.":[176],"As":[177],"compared":[178],"group,":[182],"a":[183,208],"reduction":[184],"first":[189],"evident":[190],"after":[191,211,256,265],"3":[192],"hours":[193,213,221,271],"treatment.":[195],"Continuous":[196],"culturing":[197],"longer":[201],"periods":[202],"further":[203],"reduced":[204],"TER,":[206],"maximum":[209],"treatment":[215,281],"maintained":[218],"(24":[222],"h:":[223,238],"11.86":[224],"\u00b1":[225,229,240,244,285,289,300,304,315,319,357,361,372,376,387,390,401,405,416,419,430,434,459,463,473,477],"1.19":[226],"vs.":[227,242,287,302,317,359,374,403,432,461,475],"24.13":[228],"1.26,":[230],"t":[231,246,291,306,321,363,378,392,407,421,436,465,479],"=":[232,235,247,250,292,295,307,310,322,325,364,367,379,382,393,396,408,411,422,425,437,440,466,469,480,483],"12.260,":[233],"P":[234,249,294,309,324,366,381,395,410,424,439,468,482],"0.000;":[236],"11.56":[239],"1.47":[241],"24.33":[243],"1.52,":[245],"10.460,":[248],"0.000).":[251],"At":[252],"any":[253],"time":[254],"point":[255],"adding":[257],"fluorescein,":[259],"values":[262],"significantly":[273],"higher":[274],"than":[275],"those":[276],"(20":[282],"min:":[283,298,313],"25%":[284],"3%":[286],"12%":[288],"4%,":[290],"-4.50,":[293],"0.011;":[296],"40":[297],"36%":[299],"4%":[301],"16%":[303],"5%,":[305],"-5.41,":[308],"0.006;":[311],"60":[312],"51%":[314],"5%":[316],"29%":[318],"6%,":[320],"-4.88,":[323],"0.008).":[326],"mRNA":[330],"claudin-1,":[334],"-3,":[335],"all":[339],"downregulated":[340],"H2O2,":[346],"whereas":[347],"claudin-2":[351],"upregulated":[353],"(claudin-1":[354],"mRNA:":[355,385,414],"0.98":[356],"0.18":[358],"0.28":[360],"0.12,":[362],"5.60,":[365],"0.005,":[368],"protein,":[370,428],"48":[371],"10":[373],"100":[375,404,433,476],"12,":[377],"5.77,":[380],"0.004;":[383,397],"claudin-3":[384,398],"0.37":[386],"0.12":[388],"vs.1.03":[389],"0.15,":[391],"5.95,":[394],"protein:":[399,471],"63":[400],"13":[402],"15,":[406],"3.23,":[409],"0.032;":[412],"claudin-4":[413,427],"0.38":[415],"0.11":[417],"vs.0.99":[418],"0.17,":[420],"5.22,":[423],"0.002,":[426],"57":[429],"12":[431],"13,":[435],"4.21,":[438],"0.014).":[441],"However,":[442],"these":[446],"occluding":[447],"did":[448],"not":[449],"differ":[450],"(mRNA:1.30":[458],"0.21":[460],"1.02":[462],"0.16,":[464],"-1.84,":[467],"0.140;":[470],"109":[472],"15":[474],"14,":[478],"-0.76,":[481],"0.490).":[484],"CONCLUSION:":[485],"Oxidative":[486],"stress":[487],"causes":[488],"increase":[489],"paracellular":[492],"vitro,":[498],"which":[499],"may":[500],"depends":[501],"changes":[504],"certain":[508],"transmembrane":[509],"proteins":[510],"associated":[511],"junction.":[515]},"counts_by_year":[{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-02T09:45:03.842371","created_date":"2016-06-24T00:00:00"}