{"id":"https://openalex.org/W55416773","doi":"https://doi.org/10.1007/978-1-59745-019-5_9","title":"Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures","display_name":"Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures","publication_year":2010,"publication_date":"2010-01-01","ids":{"openalex":"https://openalex.org/W55416773","doi":"https://doi.org/10.1007/978-1-59745-019-5_9","mag":"55416773","pmid":"https://pubmed.ncbi.nlm.nih.gov/20204624"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-59745-019-5_9","doi":"https://doi.org/10.1007/978-1-59745-019-5_9","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-019-5_9","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4247898593","display_name":"Mouse Cell Culture"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001792574","display_name":"Nigel J. Brand","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Nigel J. Brand","raw_affiliation_strings":["Imperial College London, National Heart & Lung Institute, Heart Science Centre, Harefield, UB9 6JH, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Imperial College London, National Heart & Lung Institute, Heart Science Centre, Harefield, UB9 6JH, UK","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075485449","display_name":"Enrique Lara\u2010Pezzi","orcid":"https://orcid.org/0000-0002-2743-1033","observed_orcids":["https://orcid.org/0000-0002-2743-1033"]},"institutions":[{"id":"https://openalex.org/I2809107791","display_name":"Spanish National Centre for Cardiovascular Research","ror":"https://ror.org/02qs1a797","country_code":"ES","type":"facility","lineage":["https://openalex.org/I2801357902","https://openalex.org/I2809107791","https://openalex.org/I4387152914"]},{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["ES","GB"],"is_corresponding":false,"raw_author_name":"Enrique Lara-Pezzi","raw_affiliation_strings":["Cardiovascular Biology Department, Fundaci\u00f3n Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, 28029, Spain","Imperial College London, National Heart & Lung Institute, Heart Science Centre, Harefield, UB9 6JH, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cardiovascular Biology Department, Fundaci\u00f3n Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, 28029, Spain","institution_ids":["https://openalex.org/I2809107791"]},{"raw_affiliation_string":"Imperial College London, National Heart & Lung Institute, Heart Science Centre, Harefield, UB9 6JH, UK","institution_ids":["https://openalex.org/I47508984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042049867","display_name":"Nadia A Rosenthal","orcid":"https://orcid.org/0000-0002-7599-7365","observed_orcids":["https://orcid.org/0000-0002-7599-7365"]},"institutions":[{"id":"https://openalex.org/I4210111367","display_name":"European Molecular Biology Laboratory","ror":"https://ror.org/01yr73893","country_code":"IT","type":"government","lineage":["https://openalex.org/I4210111367","https://openalex.org/I4210138560"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Nadia Rosenthal","raw_affiliation_strings":["EMBL Mouse Unit, Monterotondo, Rome, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"EMBL Mouse Unit, Monterotondo, Rome, Italy","institution_ids":["https://openalex.org/I4210111367"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5027925387","display_name":"Paul J.R. Barton","orcid":"https://orcid.org/0000-0002-1165-7767","observed_orcids":["https://orcid.org/0000-0002-1165-7767"]},"institutions":[{"id":"https://openalex.org/I47508984","display_name":"Imperial College London","ror":"https://ror.org/041kmwe10","country_code":"GB","type":"education","lineage":["https://openalex.org/I47508984"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Paul J.R. Barton","raw_affiliation_strings":["Imperial College London, National Heart & Lung Institute, Heart Science Centre, Harefield, UB9 6JH, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Imperial College London, National Heart & Lung Institute, Heart Science Centre, Harefield, UB9 6JH, UK","institution_ids":["https://openalex.org/I47508984"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.5544,"has_fulltext":false,"cited_by_count":23,"citation_normalized_percentile":{"value":0.82744132,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"633","issue":null,"first_page":"113","last_page":"124"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11677","display_name":"Congenital heart defects research","score":0.43479999899864197,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11677","display_name":"Congenital heart defects research","score":0.43479999899864197,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10882","display_name":"Cardiomyopathy and Myosin Studies","score":0.2371000051498413,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10217","display_name":"Cardiac electrophysiology and arrhythmias","score":0.17520000040531158,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neonatal-cardiomyocytes","display_name":"neonatal cardiomyocytes","score":0.9649999737739563},{"id":"https://openalex.org/keywords/primary-culture","display_name":"primary cell culture","score":0.75},{"id":"https://openalex.org/keywords/ventricular-myocardium","display_name":"ventricular myocardium","score":0.8619999885559082},{"id":"https://openalex.org/keywords/transgenic-mice","display_name":"transgenic mice","score":0.7730000019073486},{"id":"https://openalex.org/keywords/gene-transcription","display_name":"gene transcription","score":0.6589999794960022},{"id":"https://openalex.org/keywords/signal-transduction","display_name":"signal transduction","score":0.8939999938011169}],"concepts":[{"id":"https://openalex.org/C2778394429","wikidata":"https://www.wikidata.org/wiki/Q304956","display_name":"Collagenase","level":3,"score":0.8918380737304688},{"id":"https://openalex.org/C207200792","wikidata":"https://www.wikidata.org/wiki/Q428914","display_name":"Myocyte","level":2,"score":0.863642692565918},{"id":"https://openalex.org/C3018686881","wikidata":"https://www.wikidata.org/wiki/Q10334846","display_name":"Cardiac myocyte","level":3,"score":0.7813374996185303},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5363579392433167},{"id":"https://openalex.org/C141035611","wikidata":"https://www.wikidata.org/wiki/Q5532934","display_name":"Genetically modified mouse","level":4,"score":0.4878983199596405},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4436710476875305},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.43920257687568665},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.37608951330184937},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34200519323349},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17072051763534546},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16419383883476257},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1610962450504303},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0941748321056366},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0615198016166687}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000831","descriptor_name":"Animals, Newborn","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002448","descriptor_name":"Cell Adhesion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004210","descriptor_name":"Dissection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D008822","descriptor_name":"Mice, Transgenic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011247","descriptor_name":"Pregnancy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D032383","descriptor_name":"Myocytes, Cardiac","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D032383","descriptor_name":"Myocytes, Cardiac","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-59745-019-5_9","doi":"https://doi.org/10.1007/978-1-59745-019-5_9","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-019-5_9","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:20204624","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20204624","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6147832690","display_name":"Regeneration of the mammalian heart with cell and gene therapy","funder_award_id":"PG/10/19/28218","funder_id":"https://openalex.org/F4320319992","funder_display_name":"British Heart Foundation"}],"funders":[{"id":"https://openalex.org/F4320319992","display_name":"British Heart Foundation","ror":"https://ror.org/02wdwnk04"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W1562427827","https://openalex.org/W1585719361","https://openalex.org/W1979359086","https://openalex.org/W1982884285","https://openalex.org/W2022188551","https://openalex.org/W2033468087","https://openalex.org/W2058465745","https://openalex.org/W2091866015","https://openalex.org/W2096725497","https://openalex.org/W2098520536","https://openalex.org/W2119596286","https://openalex.org/W2139689863"],"related_works":["https://openalex.org/W1927971138","https://openalex.org/W212024047","https://openalex.org/W2373706102","https://openalex.org/W2349657784","https://openalex.org/W2362853210","https://openalex.org/W4404627084","https://openalex.org/W4405571103","https://openalex.org/W6904964021","https://openalex.org/W2354438346","https://openalex.org/W1965022856"],"abstract_inverted_index":{"We":[0],"describe":[1],"a":[2,39,94,113],"method":[3],"of":[4,10,33,58,101,115,155],"isolating":[5],"and":[6,47,55,65,103,109,128,166,171],"maintaining":[7],"primary":[8,159],"cultures":[9,26,134,162],"mouse":[11,43],"neonatal":[12],"cardiac":[13,160],"myocytes":[14,146],"(NCM).":[15],"This":[16,151],"is":[17,70],"derived":[18],"from":[19,27,80,163],"the":[20,48,59,66,99],"well-established":[21],"procedure":[22],"for":[23,112,138,157,167],"making":[24,158],"NCM":[25],"rat":[28,34],"neonates":[29,44],"by":[30],"sequential":[31],"digestion":[32,92,117],"ventricular":[35,60,68],"myocardial":[36],"pieces":[37,79],"using":[38],"collagenase/pancreatin":[40],"mixture.":[41],"One-day-old":[42],"are":[45,62,85,107,121],"taken":[46,108],"heart":[49],"excised.":[50],"The":[51,119],"great":[52],"vessels,":[53],"atria,":[54],"top":[56],"section":[57],"chambers":[61],"cut":[63,71],"away":[64],"remaining":[67],"myocardium":[69],"into":[72],"small":[73],"cubes":[74],"(about":[75],"1-2":[76],"mm(3)).":[77],"Heart":[78],"at":[81],"least":[82],"30":[83],"animals":[84],"then":[86,122],"subjected":[87],"to":[88,130,140],"short":[89],"(15-25":[90],"min)":[91],"in":[93,98],"shaking":[95],"water":[96],"bath":[97],"presence":[100],"collagenase":[102],"pancreatin.":[104],"Cell":[105],"supernatants":[106],"pooled":[110],"together":[111],"total":[114],"five":[116],"steps.":[118],"cells":[120],"plated":[123],"on":[124],"gelatinized":[125],"culture":[126],"dishes":[127],"allowed":[129],"attach":[131],"overnight.":[132],"Myocyte":[133],"were":[135],"inspected":[136],"microscopically":[137],"up":[139],"4":[141],"days,":[142],"revealing":[143],"that":[144],"many":[145],"beat":[147],"throughout":[148],"this":[149],"period.":[150],"protocol":[152],"may":[153],"be":[154],"use":[156],"myocyte":[161],"transgenic":[164],"mice":[165],"investigating":[168],"gene":[169],"transcription":[170],"cell":[172],"signalling.":[173]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}