{"id":"https://openalex.org/W55157269","doi":"https://doi.org/10.2144/01302pf01","title":"Serum-Free Recombinant Production of Adenovirus Using a Hollow Fiber Capillary System","display_name":"Serum-Free Recombinant Production of Adenovirus Using a Hollow Fiber Capillary System","publication_year":2001,"publication_date":"2001-02-01","ids":{"openalex":"https://openalex.org/W55157269","doi":"https://doi.org/10.2144/01302pf01","mag":"55157269","pmid":"https://pubmed.ncbi.nlm.nih.gov/11233612"},"language":"en","primary_location":{"id":"doi:10.2144/01302pf01","doi":"https://doi.org/10.2144/01302pf01","is_oa":true,"landing_page_url":"https://doi.org/10.2144/01302pf01","pdf_url":"https://www.future-science.com/doi/pdf/10.2144/01302pf01","source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.future-science.com/doi/pdf/10.2144/01302pf01","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027707597","display_name":"Thomas A. Gardner","orcid":"https://orcid.org/0000-0002-0124-4176","observed_orcids":["https://orcid.org/0000-0002-0124-4176"]},"institutions":[{"id":"https://openalex.org/I51556381","display_name":"University of Virginia","ror":"https://ror.org/0153tk833","country_code":"US","type":"education","lineage":["https://openalex.org/I51556381"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T.A. Gardner","raw_affiliation_strings":["Indiana University Medical Center, Indianapolis, IN","University of Virginia Health Sciences Center, Charlottesville, VA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Indiana University Medical Center, Indianapolis, IN","institution_ids":[]},{"raw_affiliation_string":"University of Virginia Health Sciences Center, Charlottesville, VA","institution_ids":["https://openalex.org/I51556381"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112830650","display_name":"Song-Chu Ko","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I51556381","display_name":"University of Virginia","ror":"https://ror.org/0153tk833","country_code":"US","type":"education","lineage":["https://openalex.org/I51556381"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S.-C. Ko","raw_affiliation_strings":["University of Virginia Health Sciences Center, Charlottesville, VA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Virginia Health Sciences Center, Charlottesville, VA","institution_ids":["https://openalex.org/I51556381"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073673612","display_name":"Ling Yang","orcid":"https://orcid.org/0000-0003-4385-0024","observed_orcids":["https://orcid.org/0000-0003-4385-0024"]},"institutions":[{"id":"https://openalex.org/I51556381","display_name":"University of Virginia","ror":"https://ror.org/0153tk833","country_code":"US","type":"education","lineage":["https://openalex.org/I51556381"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"L. Yang","raw_affiliation_strings":["University of Virginia Health Sciences Center, Charlottesville, VA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Virginia Health Sciences Center, Charlottesville, VA","institution_ids":["https://openalex.org/I51556381"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065673544","display_name":"John James Stewart Cadwell","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J.J.S. Cadwell","raw_affiliation_strings":["FiberCell Systems, New Market, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"FiberCell Systems, New Market, MD, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010686592","display_name":"Leland W.K. Chung","orcid":"https://orcid.org/0000-0003-0072-6939","observed_orcids":["https://orcid.org/0000-0003-0072-6939","https://orcid.org/0000-0001-9445-3569"]},"institutions":[{"id":"https://openalex.org/I51556381","display_name":"University of Virginia","ror":"https://ror.org/0153tk833","country_code":"US","type":"education","lineage":["https://openalex.org/I51556381"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"L.W.K. Chung","raw_affiliation_strings":["University of Virginia Health Sciences Center, Charlottesville, VA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Virginia Health Sciences Center, Charlottesville, VA","institution_ids":["https://openalex.org/I51556381"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111365848","display_name":"Chinghai H. Kao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I51556381","display_name":"University of Virginia","ror":"https://ror.org/0153tk833","country_code":"US","type":"education","lineage":["https://openalex.org/I51556381"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. Kao","raw_affiliation_strings":["Indiana University Medical Center, Indianapolis, IN","University of Virginia Health Sciences Center, Charlottesville, VA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Indiana University Medical Center, Indianapolis, IN","institution_ids":[]},{"raw_affiliation_string":"University of Virginia Health Sciences Center, Charlottesville, VA","institution_ids":["https://openalex.org/I51556381"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":0.0,"has_fulltext":true,"cited_by_count":18,"citation_normalized_percentile":{"value":0.00520963,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"30","issue":"2","first_page":"422","last_page":"427"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.7279999852180481,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.7279999852180481,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.267300009727478,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adenovirus-vectors","display_name":"adenoviral vector","score":0.9660000205039978},{"id":"https://openalex.org/keywords/serum-free-cell-culture","display_name":"serum-free cell culture","score":0.9190000295639038},{"id":"https://openalex.org/keywords/scale-up","display_name":"scale-up","score":0.46399998664855957}],"concepts":[{"id":"https://openalex.org/C519885992","wikidata":"https://www.wikidata.org/wiki/Q161","display_name":"Fiber","level":2,"score":0.5976973176002502},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5960605144500732},{"id":"https://openalex.org/C2776954459","wikidata":"https://www.wikidata.org/wiki/Q193447","display_name":"Adenoviridae","level":4,"score":0.5209135413169861},{"id":"https://openalex.org/C196806460","wikidata":"https://www.wikidata.org/wiki/Q188603","display_name":"Capillary action","level":2,"score":0.46553292870521545},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4599747657775879},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.42481833696365356},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4131106436252594},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.38618049025535583},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.3828703761100769},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3323535919189453},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3166413903236389},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.24383777379989624},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2173037827014923},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13907241821289062},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10090065002441406},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07301262021064758},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000256","descriptor_name":"Adenoviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000256","descriptor_name":"Adenoviridae","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016895","descriptor_name":"Culture Media, Serum-Free","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.2144/01302pf01","doi":"https://doi.org/10.2144/01302pf01","is_oa":true,"landing_page_url":"https://doi.org/10.2144/01302pf01","pdf_url":"https://www.future-science.com/doi/pdf/10.2144/01302pf01","source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"pmid:11233612","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11233612","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:01813a41e97e4c7d9e791112a3a2e6b5","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/01813a41e97e4c7d9e791112a3a2e6b5","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 30, Iss 2, Pp 422-427 (2001)","raw_type":"article"},{"id":"mag:3142551607","doi":null,"is_oa":false,"landing_page_url":"http://www.biotechniques.com/multimedia/archive/00010/01302pf01_10595a.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.2144/01302pf01","doi":"https://doi.org/10.2144/01302pf01","is_oa":true,"landing_page_url":"https://doi.org/10.2144/01302pf01","pdf_url":"https://www.future-science.com/doi/pdf/10.2144/01302pf01","source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.5683000087738037}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W55157269.pdf","grobid_xml":"https://content.openalex.org/works/W55157269.grobid-xml"},"referenced_works_count":7,"referenced_works":["https://openalex.org/W7834821","https://openalex.org/W1485208409","https://openalex.org/W2008071343","https://openalex.org/W2051033051","https://openalex.org/W2065623933","https://openalex.org/W4243184011","https://openalex.org/W4253616735"],"related_works":["https://openalex.org/W1910703605","https://openalex.org/W4251994943","https://openalex.org/W4388263325","https://openalex.org/W2805487117","https://openalex.org/W1533542941","https://openalex.org/W1963933842","https://openalex.org/W4231462180","https://openalex.org/W2084976117","https://openalex.org/W2336692970","https://openalex.org/W2899041754"],"abstract_inverted_index":{"A":[0],"novel":[1],"method":[2,26,77],"for":[3,78,84,96],"the":[4,51,62,79,89,93],"production":[5,80,95],"of":[6,81],"adenoviral":[7,82],"vectors":[8,83],"on":[9],"a":[10,33,57,69],"scale":[11],"sufficient":[12],"to":[13,91],"support":[14],"most":[15],"research":[16],"applications":[17],"and":[18,32,42,50,74,87],"early":[19],"phase":[20],"clinical":[21,97],"trials":[22],"is":[23,54,68],"presented.":[24],"This":[25],"utilizes":[27],"serum-free":[28],"cell":[29,36],"culture":[30,37],"medium":[31],"hollow":[34],"fiber":[35],"apparatus.":[38],"Significantly":[39],"less":[40],"time":[41],"space":[43],"are":[44],"required":[45],"than":[46],"in":[47,56],"conventional":[48],"methods,":[49],"resulting":[52],"adenovirus":[53,94],"collected":[55],"much":[58],"smaller":[59],"volume,":[60],"simplifying":[61],"purification":[63],"steps.":[64],"The":[65],"protocol":[66],"described":[67],"reproducible,":[70],"convenient,":[71],"biologically":[72],"safe,":[73],"environmentally":[75],"sound":[76],"laboratory":[85],"use":[86],"has":[88],"potential":[90],"scale-up":[92],"use.":[98]},"counts_by_year":[{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}