{"id":"https://openalex.org/W5484105","doi":"https://doi.org/10.1042/bj2340237","title":"Evidence that lysosomes are not involved in the degradation of myofibrillar proteins in rat skeletal muscle","display_name":"Evidence that lysosomes are not involved in the degradation of myofibrillar proteins in rat skeletal muscle","publication_year":1986,"publication_date":"1986-02-15","ids":{"openalex":"https://openalex.org/W5484105","doi":"https://doi.org/10.1042/bj2340237","mag":"5484105","pmid":"https://pubmed.ncbi.nlm.nih.gov/3707546","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1146553"},"language":"en","primary_location":{"id":"doi:10.1042/bj2340237","doi":"https://doi.org/10.1042/bj2340237","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj2340237","pdf_url":"https://portlandpress.com/biochemj/article-pdf/234/1/237/586121/bj2340237.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W5484105"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://portlandpress.com/biochemj/article-pdf/234/1/237/586121/bj2340237.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5024782501","display_name":"Bradford B. Lowell","orcid":"https://orcid.org/0000-0002-0436-3760","observed_orcids":["https://orcid.org/0000-0002-0436-3760"]},"institutions":[{"id":"https://openalex.org/I111088046","display_name":"Boston University","ror":"https://ror.org/05qwgg493","country_code":"US","type":"education","lineage":["https://openalex.org/I111088046"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"B B Lowell","raw_affiliation_strings":["Departments of *Medicine and tPhysiology, Boston University School of Medicine, Boston, MA 02118, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of *Medicine and tPhysiology, Boston University School of Medicine, Boston, MA 02118, U.S.A","institution_ids":["https://openalex.org/I111088046"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055882378","display_name":"Neil B. Ruderman","orcid":"https://orcid.org/0000-0002-6589-6587","observed_orcids":["https://orcid.org/0000-0002-6589-6587"]},"institutions":[{"id":"https://openalex.org/I111088046","display_name":"Boston University","ror":"https://ror.org/05qwgg493","country_code":"US","type":"education","lineage":["https://openalex.org/I111088046"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"N B Ruderman","raw_affiliation_strings":["Departments of *Medicine and tPhysiology, Boston University School of Medicine, Boston, MA 02118, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of *Medicine and tPhysiology, Boston University School of Medicine, Boston, MA 02118, U.S.A","institution_ids":["https://openalex.org/I111088046"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103367307","display_name":"M. N. Goodman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111088046","display_name":"Boston University","ror":"https://ror.org/05qwgg493","country_code":"US","type":"education","lineage":["https://openalex.org/I111088046"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M N Goodman","raw_affiliation_strings":["Departments of *Medicine and tPhysiology, Boston University School of Medicine, Boston, MA 02118, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of *Medicine and tPhysiology, Boston University School of Medicine, Boston, MA 02118, U.S.A","institution_ids":["https://openalex.org/I111088046"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I111088046"],"apc_list":null,"apc_paid":null,"fwci":15.7982,"has_fulltext":true,"cited_by_count":222,"citation_normalized_percentile":{"value":0.99708455,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"234","issue":"1","first_page":"237","last_page":"240"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11399","display_name":"Muscle metabolism and nutrition","score":0.7979000210762024,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11399","display_name":"Muscle metabolism and nutrition","score":0.7979000210762024,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.16380000114440918,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.0066999997943639755,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lysosomes","display_name":"lysosomes","score":0.925000011920929},{"id":"https://openalex.org/keywords/myofibrillar-protein-degradation","display_name":"myofibrillar protein degradation","score":0.9190000295639038},{"id":"https://openalex.org/keywords/skeletal-muscle","display_name":"skeletal muscle","score":0.9509999752044678},{"id":"https://openalex.org/keywords/lysosomal-proteases","display_name":"lysosomal proteases","score":0.8080000281333923},{"id":"https://openalex.org/keywords/starvation","display_name":"starvation","score":0.7519999742507935},{"id":"https://openalex.org/keywords/protein-degradation","display_name":"protein degradation","score":0.5070000290870667},{"id":"https://openalex.org/keywords/insulin","display_name":"insulin","score":0.5860000252723694},{"id":"https://openalex.org/keywords/amino-acids","display_name":"amino acids","score":0.9319999814033508}],"concepts":[{"id":"https://openalex.org/C7301908","wikidata":"https://www.wikidata.org/wiki/Q318623","display_name":"Myofibril","level":2,"score":0.9402294158935547},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.7485803365707397},{"id":"https://openalex.org/C2779959927","wikidata":"https://www.wikidata.org/wiki/Q1048687","display_name":"Skeletal muscle","level":2,"score":0.6268405318260193},{"id":"https://openalex.org/C197683979","wikidata":"https://www.wikidata.org/wiki/Q14876077","display_name":"Protein catabolism","level":3,"score":0.5941743850708008},{"id":"https://openalex.org/C2992195973","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Protein degradation","level":2,"score":0.5796530246734619},{"id":"https://openalex.org/C2776165026","wikidata":"https://www.wikidata.org/wiki/Q188017","display_name":"Tyrosine","level":2,"score":0.5569543242454529},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.5010042190551758},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4861986041069031},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.485446035861969},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.47124701738357544},{"id":"https://openalex.org/C2780216420","wikidata":"https://www.wikidata.org/wiki/Q83330","display_name":"Lysosome","level":3,"score":0.45282867550849915},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.3440425992012024},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32263290882110596},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.24465259909629822},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.07189148664474487}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008247","descriptor_name":"Lysosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008762","descriptor_name":"Methylhistidines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009124","descriptor_name":"Muscle Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009210","descriptor_name":"Myofibrils","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D009210","descriptor_name":"Myofibrils","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011919","descriptor_name":"Rats, Inbred Strains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013217","descriptor_name":"Starvation","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014443","descriptor_name":"Tyrosine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1042/bj2340237","doi":"https://doi.org/10.1042/bj2340237","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj2340237","pdf_url":"https://portlandpress.com/biochemj/article-pdf/234/1/237/586121/bj2340237.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},{"id":"pmid:3707546","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3707546","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Biochemical journal","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1146553","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC1146553/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochem J","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1042/bj2340237","doi":"https://doi.org/10.1042/bj2340237","is_oa":true,"landing_page_url":"https://doi.org/10.1042/bj2340237","pdf_url":"https://portlandpress.com/biochemj/article-pdf/234/1/237/586121/bj2340237.pdf","source":{"id":"https://openalex.org/S44425019","display_name":"Biochemical Journal","issn_l":"0264-6021","issn":["0264-6021","1470-8728"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310311608","host_organization_name":"Portland Press","host_organization_lineage":["https://openalex.org/P4310311608","https://openalex.org/P4310311715"],"host_organization_lineage_names":["Portland Press","Biochemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemical Journal","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2059022924","display_name":null,"funder_award_id":"AM-19514","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G2080435607","display_name":"PROTEIN SPARING IN STARVATION--EFFECT OF AGE &OBESITY","funder_award_id":"5r01am019469-09","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G435405106","display_name":null,"funder_award_id":"AM 00652","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332505","display_name":"U.S. Public Health Service","ror":"https://ror.org/05xf94514"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W5484105.pdf","grobid_xml":"https://content.openalex.org/works/W5484105.grobid-xml"},"referenced_works_count":26,"referenced_works":["https://openalex.org/W277249932","https://openalex.org/W1491317952","https://openalex.org/W1891164690","https://openalex.org/W1977150324","https://openalex.org/W1977286678","https://openalex.org/W1978986745","https://openalex.org/W1979198879","https://openalex.org/W1996297374","https://openalex.org/W2023550291","https://openalex.org/W2036845363","https://openalex.org/W2036930352","https://openalex.org/W2055944101","https://openalex.org/W2084547059","https://openalex.org/W2095894333","https://openalex.org/W2126787546","https://openalex.org/W2204008620","https://openalex.org/W2272777185","https://openalex.org/W2275486780","https://openalex.org/W2284242947","https://openalex.org/W2401436972","https://openalex.org/W2403229825","https://openalex.org/W2409791365","https://openalex.org/W2417304169","https://openalex.org/W2418458599","https://openalex.org/W6604929115","https://openalex.org/W6756447727"],"related_works":["https://openalex.org/W2055944101","https://openalex.org/W2345499296","https://openalex.org/W2149979005","https://openalex.org/W126860879","https://openalex.org/W2323500496","https://openalex.org/W2408431894","https://openalex.org/W2316710672","https://openalex.org/W1947295314","https://openalex.org/W2038984433","https://openalex.org/W2460098961"],"abstract_inverted_index":{"To":[0],"examine":[1],"the":[2,7,15,29,45,67,101,127],"role":[3],"of":[4,9,17,23,33,43,47,53,85,93,103,109,118,130],"lysosomes":[5],"in":[6,28,153],"degradation":[8],"skeletal-muscle":[10],"myofibrillar":[11,131],"proteins,":[12],"we":[13],"measured":[14],"release":[16,46,102],"N":[18,48,104],"tau-methylhistidine":[19,49,105],"from":[20],"perfused":[21,51,107],"muscle":[22,52,108],"starved":[24,110],"and":[25,56,63,81,111],"fed":[26,112],"rats":[27,58],"presence":[30],"or":[31,95],"absence":[32],"agents":[34],"that":[35,126,141],"inhibit":[36],"lysosomal":[37,86],"proteinase":[38],"activity.":[39],"After":[40],"1":[41],"day":[42],"starvation,":[44],"by":[50,60,74,78,106],"4-,":[54],"8-":[55],"24-week-old":[57],"increased":[59,77],"322,":[61],"159":[62],"134%":[64],"respectively.":[65,83],"On":[66],"other":[68],"hand,":[69],"total":[70,119,142],"protein":[71,120,148],"breakdown,":[72,149],"assessed":[73],"tyrosine":[75],"release,":[76],"62,":[79],"20":[80],"20%":[82],"Inhibitors":[84],"proteinases":[87],"as":[88,90],"well":[89],"high":[91],"concentrations":[92],"insulin":[94],"amino":[96],"acids":[97],"failed":[98],"to":[99],"diminish":[100],"rats,":[113],"despite":[114],"a":[115,135],"25-35%":[116],"inhibition":[117],"breakdown.":[121],"The":[122],"data":[123],"strongly":[124],"suggest":[125,140],"complete":[128],"breakdown":[129],"proteins":[132],"occurs":[133,150],"via":[134],"non-lysosomal":[136],"pathway.":[137],"They":[138],"also":[139],"proteolysis,":[143],"which":[144],"primarily":[145],"reflects":[146],"non-myofibrillar":[147],"at":[151],"least":[152],"part":[154],"within":[155],"lysosomes.":[156]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}