{"id":"https://openalex.org/W54546147","doi":null,"title":"Characterization of the 70,000 dalton heat shock proteins of Euglena gracilis","display_name":"Characterization of the 70,000 dalton heat shock proteins of Euglena gracilis","publication_year":1989,"publication_date":"1989-04-01","ids":{"openalex":"https://openalex.org/W54546147","mag":"54546147"},"language":"en","primary_location":{"id":"mag:54546147","doi":null,"is_oa":false,"landing_page_url":"http://www.osti.gov/scitech/biblio/6644105","pdf_url":null,"source":{"id":"https://openalex.org/S4306522614","display_name":"Plant Physiology, Supplement; (USA)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Plant Physiology, Supplement; (USA)","raw_type":null},"volume":null,"type":"article","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076703398","display_name":"Thomas Leustek","orcid":"https://orcid.org/0000-0002-8479-6557","observed_orcids":["https://orcid.org/0000-0002-8479-6557"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Thomas Leustek","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-8479-6557","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038074267","display_name":"Dekel Amir-Shapira","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dekel Amir-Shapira","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088128263","display_name":"Barbara L. Dalie","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Barbara Dalie","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041730312","display_name":"Herbert Weissbach","orcid":"https://orcid.org/0000-0002-8977-084X","observed_orcids":["https://orcid.org/0000-0002-8977-084X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Herbert Weissbach","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110074559","display_name":"N Brot","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"N Brot","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5109146473","display_name":"Welch Wj","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Welch Wj","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0022988,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heat-shock-proteins","display_name":"heat shock proteins","score":0.8999999761581421},{"id":"https://openalex.org/keywords/euglena-gracilis","display_name":"Euglena gracilis","score":0.8840000033378601},{"id":"https://openalex.org/keywords/hsp70","display_name":"HSP70","score":0.8539999723434448},{"id":"https://openalex.org/keywords/heat-shock-response","display_name":"heat shock response","score":0.9350000023841858},{"id":"https://openalex.org/keywords/protein-synthesis","display_name":"protein synthesis","score":0.699999988079071},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.7239999771118164},{"id":"https://openalex.org/keywords/nuclear-cytoplasmic-localization","display_name":"nuclear-cytoplasmic localization","score":0.6890000104904175},{"id":"https://openalex.org/keywords/atpase-activity","display_name":"ATPase activity","score":0.871999979019165},{"id":"https://openalex.org/keywords/protein-phosphorylation","display_name":"protein phosphorylation","score":0.9010000228881836},{"id":"https://openalex.org/keywords/calcium-dependent-phosphorylation","display_name":"calcium-dependent phosphorylation","score":0.7760000228881836}],"concepts":[{"id":"https://openalex.org/C2780472036","wikidata":"https://www.wikidata.org/wiki/Q309852","display_name":"Euglena gracilis","level":4,"score":0.833467960357666},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5509037375450134},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.5354784727096558},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5184513926506042},{"id":"https://openalex.org/C2780437362","wikidata":"https://www.wikidata.org/wiki/Q236001","display_name":"Euglena","level":4,"score":0.4927809536457062},{"id":"https://openalex.org/C205260736","wikidata":"https://www.wikidata.org/wiki/Q418939","display_name":"Heat shock protein","level":3,"score":0.48758333921432495},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.4628635346889496},{"id":"https://openalex.org/C69305403","wikidata":"https://www.wikidata.org/wiki/Q47263","display_name":"Chloroplast","level":3,"score":0.461757630109787},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44812530279159546},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.4180336892604828},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.412426620721817},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.38217073678970337},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07358509302139282},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:54546147","doi":null,"is_oa":false,"landing_page_url":"http://www.osti.gov/scitech/biblio/6644105","pdf_url":null,"source":{"id":"https://openalex.org/S4306522614","display_name":"Plant Physiology, Supplement; (USA)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Plant Physiology, Supplement; (USA)","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2085648943","https://openalex.org/W2028767374","https://openalex.org/W1977537470","https://openalex.org/W2091025904","https://openalex.org/W2060974318","https://openalex.org/W2321651359","https://openalex.org/W2138629856","https://openalex.org/W1897990850","https://openalex.org/W1972300355","https://openalex.org/W1987723016"],"abstract_inverted_index":{"The":[0,36,115],"heat":[1,88,118],"shock":[2,89],"response":[3],"of":[4,38,40,106,166],"Euglena":[5],"gracilis":[6],"was":[7,121],"studied":[8],"and":[9,26,45,60,68,85,95,126,144,169],"the":[10,27,92,104,108,124,130,157,164],"proteins":[11,28,98,147,171],"synthesized":[12,29],"were":[13,16,30,71,99],"identified.":[14],"Cells":[15],"pulse-labeled":[17],"with":[18,78],"({sup":[19],"35}S)":[20],"sulfate":[21],"at":[22,41,101,113,135],"21{degrees}C":[23,136],"or":[24,137],"36{degrees}C":[25],"analyzed":[31],"by":[32,75],"acrylamide":[33],"gel":[34],"analysis.":[35],"rate":[37],"synthesis":[39,105],"least":[42],"4":[43],"major":[44],"6":[46],"minor":[47],"polypeptides,":[48,65],"ranging":[49],"from":[50],"25":[51],"Kd":[52,55,67,87,94,97,110,117,143,146,159],"to":[53,73,139],"116":[54],"increased":[56,112],"in":[57,123,132,163],"both":[58],"light":[59],"dark":[61],"grown":[62,134],"cultures.":[63],"Two":[64],"68":[66,93,109,116,142,158],"69":[69,96,145],"Kd,":[70],"found":[72],"cross-react":[74],"immunoblot":[76],"analysis":[77],"monoclonal":[79],"antibodies":[80],"raised":[81],"against":[82],"human":[83],"72":[84],"73":[86],"proteins.":[90],"Both":[91,141],"expressed":[100],"21{degrees}C;":[102],"however,":[103],"only":[107],"protein":[111,120,160],"36{degrees}C.":[114,140],"inducible":[119],"present":[122],"cytoplasm":[125],"nucleus,":[127],"but":[128],"not":[129],"chloroplast,":[131],"cells":[133],"exposed":[138],"copurified":[148],"during":[149],"ATP-agarose":[150],"affinity":[151],"chromatography.":[152],"Preliminary":[153],"evidence":[154],"indicates":[155],"that":[156],"is":[161],"phosphorylated":[162],"presence":[165],"Ca{sup":[167],"++}":[168],"these":[170],"exhibit":[172],"ATPase":[173],"activity.":[174]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}