{"id":"https://openalex.org/W54512339","doi":null,"title":"Optimization of parameters for isolation of protoplasts from Gracilaria verrucosa (Rhodophyta).","display_name":"Optimization of parameters for isolation of protoplasts from Gracilaria verrucosa (Rhodophyta).","publication_year":1998,"publication_date":"1998-08-01","ids":{"openalex":"https://openalex.org/W54512339","mag":"54512339","pmid":"https://pubmed.ncbi.nlm.nih.gov/9701645"},"language":"en","primary_location":{"id":"pmid:9701645","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9701645","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of marine biotechnology","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Araki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I178574317","display_name":"Mie University","ror":"https://ror.org/01529vy56","country_code":"JP","type":"education","lineage":["https://openalex.org/I178574317"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Araki","raw_affiliation_strings":["Department of Chemistry of Fishery Resources, Faculty of Bioresources, Mie University, 1515 Kamihama Tsu 514, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry of Fishery Resources, Faculty of Bioresources, Mie University, 1515 Kamihama Tsu 514, Japan","institution_ids":["https://openalex.org/I178574317"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Lu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"Morishita","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Morishita","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":56,"citation_normalized_percentile":{"value":0.00483019,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"6","issue":"3","first_page":"193","last_page":"7"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10643","display_name":"Marine and coastal plant biology","score":0.6977999806404114,"subfield":{"id":"https://openalex.org/subfields/1910","display_name":"Oceanography"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10643","display_name":"Marine and coastal plant biology","score":0.6977999806404114,"subfield":{"id":"https://openalex.org/subfields/1910","display_name":"Oceanography"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.1103999987244606,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11390","display_name":"Seaweed-derived Bioactive Compounds","score":0.04879999905824661,"subfield":{"id":"https://openalex.org/subfields/1104","display_name":"Aquatic Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gracilaria-verrucosa","display_name":"Gracilaria verrucosa","score":0.9559999704360962},{"id":"https://openalex.org/keywords/protoplast-isolation","display_name":"protoplast isolation","score":0.8740000128746033},{"id":"https://openalex.org/keywords/agarase","display_name":"agarase","score":0.9419999718666077},{"id":"https://openalex.org/keywords/polysaccharide-degrading-enzymes","display_name":"polysaccharide-degrading enzymes","score":0.9459999799728394}],"concepts":[{"id":"https://openalex.org/C173749725","wikidata":"https://www.wikidata.org/wiki/Q1126500","display_name":"Protoplast","level":2,"score":0.9227708578109741},{"id":"https://openalex.org/C141603559","wikidata":"https://www.wikidata.org/wiki/Q410324","display_name":"Cellulase","level":3,"score":0.9171721935272217},{"id":"https://openalex.org/C2778445172","wikidata":"https://www.wikidata.org/wiki/Q407646","display_name":"Mannitol","level":2,"score":0.8085023164749146},{"id":"https://openalex.org/C2779832904","wikidata":"https://www.wikidata.org/wiki/Q410420","display_name":"Papain","level":3,"score":0.7850965857505798},{"id":"https://openalex.org/C2775936685","wikidata":"https://www.wikidata.org/wiki/Q3087307","display_name":"Gracilaria","level":3,"score":0.6284850835800171},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4935969114303589},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.471798837184906},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4694235920906067},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.46580344438552856},{"id":"https://openalex.org/C100817775","wikidata":"https://www.wikidata.org/wiki/Q134219","display_name":"Polysaccharide","level":2,"score":0.4466427266597748},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.4123344123363495},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.3963962495326996},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3767288327217102},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.297467976808548},{"id":"https://openalex.org/C559758991","wikidata":"https://www.wikidata.org/wiki/Q37868","display_name":"Algae","level":2,"score":0.25087034702301025}],"mesh":[],"locations_count":2,"locations":[{"id":"pmid:9701645","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9701645","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of marine biotechnology","raw_type":"Journal Article"},{"id":"mag:54512339","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9701645","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2138200843","https://openalex.org/W2061482145","https://openalex.org/W2023333774","https://openalex.org/W1967632137","https://openalex.org/W2059881636","https://openalex.org/W2089840257","https://openalex.org/W2065318316","https://openalex.org/W2061203468","https://openalex.org/W2052715908","https://openalex.org/W2126487177"],"abstract_inverted_index":{"A":[0],"systematic":[1],"method":[2],"was":[3,78,111],"designed":[4],"for":[5,48,64,142],"the":[6,40,57,65,87],"isolation":[7],"of":[8,12,39,93,118,121],"a":[9,23],"large":[10],"number":[11],"protoplasts":[13,151],"from":[14,22],"an":[15,82,114],"agarophyte":[16],"alga":[17],"Gracilaria":[18],"verrucosa":[19,95],"using":[20],"agarase":[21],"marine":[24],"bacterium":[25],"Vibrio":[26],"sp.":[27],"PO-303":[28],"and":[29,35,62,71,128],"commercial":[30],"enzymes":[31,55],"(Cellulase":[32],"Onozuka":[33],"RS":[34],"Macerozyme":[36],"R-10).":[37],"Pretreatment":[38],"tissue":[41,88],"with":[42,53,97,113,139],"5%":[43,98],"papain":[44,99],"at":[45,145],"22":[46,72,146],"degreesC":[47],"30":[49],"min":[50,144],"before":[51],"digestion":[52],"polysaccharide-degrading":[54,66],"increased":[56],"protoplast":[58],"yield.":[59],"Suitable":[60],"pH":[61,105],"temperature":[63],"enzyme":[67,115],"reaction":[68],"were":[69,152],"6.5":[70],"degreesC,":[73,147],"respectively.":[74],"Mannitol":[75],"(0.7":[76],"M)":[77],"found":[79],"to":[80],"be":[81],"excellent":[83],"osmotic":[84],"stabilizer.":[85],"When":[86],"(1":[89],"g,":[90],"fresh":[91],"wt.)":[92],"G.":[94],"pretreated":[96],"solution":[100],"(20":[101],"mM":[102,134],"MES":[103,135],"buffer,":[104],"7.5,":[106],"containing":[107],"0.7":[108,129],"M":[109,130],"mannitol)":[110],"digested":[112],"mixture":[116],"consisting":[117],"4":[119],"units":[120],"agarase,":[122],"4%":[123],"Cellulase":[124],"Onozuka,":[125],"2%":[126],"Macerozyme,":[127],"mannitol":[131],"in":[132],"20":[133],"buffer":[136],"(pH":[137],"6.5)":[138],"gentle":[140],"agitation":[141],"150":[143],"1.03":[148],"x":[149],"10(8)":[150],"obtained.":[153]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":5}],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2016-06-24T00:00:00"}
