{"id":"https://openalex.org/W4992181","doi":null,"title":"Primary culture of rat cerebral microvascular endothelial cells. Isolation, growth, and characterization.","display_name":"Primary culture of rat cerebral microvascular endothelial cells. Isolation, growth, and characterization.","publication_year":1982,"publication_date":"1982-06-01","ids":{"openalex":"https://openalex.org/W4992181","mag":"4992181","pmid":"https://pubmed.ncbi.nlm.nih.gov/7045518"},"language":"en","primary_location":{"id":"pmid:7045518","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7045518","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Laboratory investigation; a journal of technical methods and pathology","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5018091007","display_name":"Clement A. Diglio","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C A Diglio","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088789109","display_name":"Paula Grammas","orcid":"https://orcid.org/0000-0002-2636-3550","observed_orcids":["https://orcid.org/0000-0002-2636-3550"]},"institutions":[{"id":"https://openalex.org/I185443292","display_name":"Wayne State University","ror":"https://ror.org/01070mq45","country_code":"US","type":"education","lineage":["https://openalex.org/I185443292"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P Grammas","raw_affiliation_strings":["Wayne State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wayne State University","institution_ids":["https://openalex.org/I185443292"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002161137","display_name":"Filiberto Giacomelli","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185443292","display_name":"Wayne State University","ror":"https://ror.org/01070mq45","country_code":"US","type":"education","lineage":["https://openalex.org/I185443292"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"F Giacomelli","raw_affiliation_strings":["Wayne State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wayne State University","institution_ids":["https://openalex.org/I185443292"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5053286483","display_name":"Joseph Wiener","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185443292","display_name":"Wayne State University","ror":"https://ror.org/01070mq45","country_code":"US","type":"education","lineage":["https://openalex.org/I185443292"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Wiener","raw_affiliation_strings":["Wayne State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wayne State University","institution_ids":["https://openalex.org/I185443292"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.2035,"has_fulltext":false,"cited_by_count":138,"citation_normalized_percentile":{"value":0.93381389,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"46","issue":"6","first_page":"554","last_page":"63"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11872","display_name":"Barrier Structure and Function Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11872","display_name":"Barrier Structure and Function Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10422","display_name":"Angiogenesis and VEGF in Cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/brain-microvascular-endothelial-cells","display_name":"brain microvascular endothelial cells","score":0.9890000224113464},{"id":"https://openalex.org/keywords/primary-culture","display_name":"primary cell culture","score":0.8889999985694885},{"id":"https://openalex.org/keywords/endothelial-cell-isolation","display_name":"endothelial cell isolation","score":0.7829999923706055},{"id":"https://openalex.org/keywords/collagenase-digestion","display_name":"collagenase digestion","score":0.7860000133514404},{"id":"https://openalex.org/keywords/factor-viii","display_name":"factor VIII","score":0.6000000238418579},{"id":"https://openalex.org/keywords/angiotensin-converting-enzyme","display_name":"angiotensin-converting enzyme","score":0.9959999918937683},{"id":"https://openalex.org/keywords/smooth-muscle-myosin","display_name":"smooth muscle myosin","score":0.8759999871253967},{"id":"https://openalex.org/keywords/gap-junctions","display_name":"gap junctions","score":0.7799999713897705},{"id":"https://openalex.org/keywords/tight-junctions","display_name":"tight junctions","score":1.0},{"id":"https://openalex.org/keywords/endothelial-cell-morphology","display_name":"endothelial cell morphology","score":0.5260000228881836},{"id":"https://openalex.org/keywords/brain-capillary-endothelium","display_name":"brain capillary endothelium","score":0.8149999976158142},{"id":"https://openalex.org/keywords/rat-cerebral-cortex","display_name":"rat cortex","score":0.6209999918937683}],"concepts":[{"id":"https://openalex.org/C123012128","wikidata":"https://www.wikidata.org/wiki/Q5376430","display_name":"Endothelial stem cell","level":3,"score":0.7662665843963623},{"id":"https://openalex.org/C2776992346","wikidata":"https://www.wikidata.org/wiki/Q111140","display_name":"Endothelium","level":2,"score":0.7080507278442383},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6129447221755981},{"id":"https://openalex.org/C2778939058","wikidata":"https://www.wikidata.org/wiki/Q4046167","display_name":"CD31","level":3,"score":0.5563960671424866},{"id":"https://openalex.org/C2776436680","wikidata":"https://www.wikidata.org/wiki/Q1632335","display_name":"Microvessel","level":3,"score":0.5392938852310181},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.47956913709640503},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4732323884963989},{"id":"https://openalex.org/C2780394083","wikidata":"https://www.wikidata.org/wiki/Q539568","display_name":"Angiogenesis","level":2,"score":0.4521035850048065},{"id":"https://openalex.org/C2778394429","wikidata":"https://www.wikidata.org/wiki/Q304956","display_name":"Collagenase","level":3,"score":0.4402342438697815},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.41148820519447327},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.40779048204421997},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.27601805329322815},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.16277718544006348},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13017547130584717},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.11771148443222046},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002196","descriptor_name":"Capillaries","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002560","descriptor_name":"Cerebrovascular Circulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004727","descriptor_name":"Endothelium","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D005455","descriptor_name":"Fluorescent Antibody Technique","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008855","descriptor_name":"Microscopy, Electron, Scanning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011919","descriptor_name":"Rats, Inbred Strains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:7045518","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7045518","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Laboratory investigation; a journal of technical methods and pathology","raw_type":"Journal Article"},{"id":"mag:4992181","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7045518","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8560000061988831}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2386561880","https://openalex.org/W2009902709","https://openalex.org/W2036004582","https://openalex.org/W2414796754","https://openalex.org/W2371195261","https://openalex.org/W235851718","https://openalex.org/W1983236757","https://openalex.org/W2360610956","https://openalex.org/W2336536176","https://openalex.org/W2348583313"],"abstract_inverted_index":{"A":[0],"procedure":[1],"for":[2,113,143],"the":[3,76,86,187],"isolation":[4,47],"and":[5,38,48,59,70,125,147,150,177,195,199],"long-term":[6,181],"in":[7,34,196,200,219],"vitro":[8],"cultivation":[9],"of":[10,61,75,88,96,127,134,152,154,162,172,203,222],"endothelial":[11,62,77,89,103,111,132,164,182,192],"cells":[12,21,63,78,136],"from":[13,23,186],"rat":[14,155],"cerebral":[15,188],"cortical":[16],"microvasculature":[17,189],"is":[18],"described.":[19],"Migrating":[20],"emerged":[22],"collagenase-treated":[24],"microvessel":[25],"fragments":[26],"as":[27,29,64,210],"early":[28],"1":[30],"to":[31,43,51,53,83,115,215],"2":[32],"days":[33,45],"culture.":[35],"Migration":[36],"continued":[37,49],"marked":[39],"proliferation":[40],"began":[41],"5":[42],"7":[44],"after":[46,118],"up":[50],"12":[52],"14":[54],"days.":[55],"Cell":[56],"colonies":[57],"developed":[58],"consisted":[60],"determined":[65],"by":[66,139],"phase":[67],"contrast":[68],"microscopy":[69],"cell":[71,90,104,165,183,193],"culture":[72],"behavior.":[73],"Proliferation":[74],"was":[79,137],"significantly":[80],"enhanced":[81],"(3-":[82],"4-fold)":[84],"with":[85,102],"addition":[87],"growth":[91,105],"supplement":[92,106],"at":[93],"a":[94,122,211,220],"concentration":[95],"150":[97],"microgram.":[98],"per":[99],"ml.":[100],"Cultures":[101],"(ECGS)":[107],"retained":[108],"their":[109,128],"characteristic":[110,191],"morphology":[112],"6":[114],"8":[116],"weeks,":[117],"which":[119],"they":[120,207],"exhibited":[121],"gradual":[123],"deterioration":[124],"loss":[126],"phenotypic":[129],"appearance.":[130],"The":[131],"origin":[133],"these":[135],"demonstrated":[138],"positive":[140],"immunofluorescent":[141],"staining":[142],"factor":[144],"VIII":[145],"antigen":[146],"angiotensin-converting":[148],"enzyme":[149],"lack":[151],"binding":[153],"smooth":[156],"muscle":[157],"myosin":[158],"antibody.":[159],"Ultrastructural":[160],"examination":[161],"confluent":[163],"cultures":[166,184],"revealed":[167],"intercellular":[168],"junctional":[169],"complexes":[170],"consisting":[171],"gap":[173],"junctions,":[174],"punctate":[175],"fusions,":[176],"tight":[178],"junctions.":[179],"Since":[180],"derived":[185],"retain":[190],"markers":[194,198],"vivo":[197,201],"features":[202],"brain":[204],"capillary":[205],"endothelium,":[206],"can":[208],"serve":[209],"useful":[212],"model":[213],"system":[214],"characterize":[216],"microvascular":[217],"endothelium":[218],"variety":[221],"disease":[223],"states.":[224]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
