{"id":"https://openalex.org/W48028041","doi":null,"title":"Withaferin A Targets Hsp90 in Pancreatic Cancer Cells.","display_name":"Withaferin A Targets Hsp90 in Pancreatic Cancer Cells.","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W48028041","mag":"48028041"},"language":"en","primary_location":{"id":"pmh:oai:deepblue.lib.umich.edu:2027.42/89629","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/2027.42/89629","pdf_url":"https://hdl.handle.net/2027.42/89629","source":{"id":"https://openalex.org/S4306400393","display_name":"Deep Blue (University of Michigan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I27837315","host_organization_name":"University of Michigan","host_organization_lineage":["https://openalex.org/I27837315"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://hdl.handle.net/2027.42/89629","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101745077","display_name":"Yanke Yu","orcid":"https://orcid.org/0000-0002-3276-0130","observed_orcids":["https://orcid.org/0000-0002-3276-0130"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Yanke Yu","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-3276-0130","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5101745077"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.5922999978065491,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.5922999978065491,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12298","display_name":"Phytochemicals and Medicinal Plants","score":0.39309999346733093,"subfield":{"id":"https://openalex.org/subfields/2707","display_name":"Complementary and alternative medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10231","display_name":"Pancreatic and Hepatic Oncology Research","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/withaferin-a","display_name":"withaferin A","score":0.9679999947547913},{"id":"https://openalex.org/keywords/hsp90-inhibition","display_name":"HSP90 inhibition","score":0.9739999771118164},{"id":"https://openalex.org/keywords/pancreatic-cancer","display_name":"pancreatic cancer","score":0.9919999837875366},{"id":"https://openalex.org/keywords/withanolides","display_name":"withanolides","score":0.777999997138977},{"id":"https://openalex.org/keywords/hsp70","display_name":"HSP70","score":0.703000009059906},{"id":"https://openalex.org/keywords/myricetin","display_name":"myricetin","score":0.9599999785423279},{"id":"https://openalex.org/keywords/hsp90-client-proteins","display_name":"Hsp90 client proteins","score":0.7749999761581421},{"id":"https://openalex.org/keywords/akt","display_name":"Akt","score":0.703000009059906},{"id":"https://openalex.org/keywords/cdk4","display_name":"CDK4","score":0.9940000176429749},{"id":"https://openalex.org/keywords/glucocorticoid-receptor","display_name":"glucocorticoid receptor","score":0.9559999704360962},{"id":"https://openalex.org/keywords/structure-activity-relationship","display_name":"structure-activity relationship","score":0.8050000071525574},{"id":"https://openalex.org/keywords/synergistic-drug-combination","display_name":"synergistic drug combination","score":0.7160000205039978},{"id":"https://openalex.org/keywords/tumor-growth-inhibition","display_name":"tumor growth inhibition","score":0.6370000243186951},{"id":"https://openalex.org/keywords/natural-products","display_name":"natural products","score":0.4519999921321869},{"id":"https://openalex.org/keywords/ashwagandha","display_name":"Withania somnifera","score":0.8899999856948853}],"concepts":[{"id":"https://openalex.org/C2776598214","wikidata":"https://www.wikidata.org/wiki/Q6606395","display_name":"Withaferin A","level":4,"score":0.7672246694564819},{"id":"https://openalex.org/C2780210213","wikidata":"https://www.wikidata.org/wiki/Q212961","display_name":"Pancreatic cancer","level":3,"score":0.6770154237747192},{"id":"https://openalex.org/C2775932338","wikidata":"https://www.wikidata.org/wiki/Q24772687","display_name":"Hsp90","level":4,"score":0.4977417290210724},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.4746288061141968},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36884474754333496},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.3565729260444641},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.349856972694397},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.28816869854927063},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.203078955411911},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12929165363311768},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.1283833384513855},{"id":"https://openalex.org/C205260736","wikidata":"https://www.wikidata.org/wiki/Q418939","display_name":"Heat shock protein","level":3,"score":0.05434414744377136},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.04717230796813965},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0},{"id":"https://openalex.org/C2780069950","wikidata":"https://www.wikidata.org/wiki/Q852660","display_name":"Withania somnifera","level":3,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:deepblue.lib.umich.edu:2027.42/89629","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/2027.42/89629","pdf_url":"https://hdl.handle.net/2027.42/89629","source":{"id":"https://openalex.org/S4306400393","display_name":"Deep Blue (University of Michigan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I27837315","host_organization_name":"University of Michigan","host_organization_lineage":["https://openalex.org/I27837315"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:48028041","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/2027.42/89629","pdf_url":null,"source":{"id":"https://openalex.org/S4306400393","display_name":"Deep Blue (University of Michigan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I27837315","host_organization_name":"University of Michigan","host_organization_lineage":["https://openalex.org/I27837315"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Deep Blue (University of Michigan)","raw_type":null}],"best_oa_location":{"id":"pmh:oai:deepblue.lib.umich.edu:2027.42/89629","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/2027.42/89629","pdf_url":"https://hdl.handle.net/2027.42/89629","source":{"id":"https://openalex.org/S4306400393","display_name":"Deep Blue (University of Michigan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I27837315","host_organization_name":"University of Michigan","host_organization_lineage":["https://openalex.org/I27837315"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9537000060081482}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W48028041.pdf","grobid_xml":"https://content.openalex.org/works/W48028041.grobid-xml"},"referenced_works_count":215,"referenced_works":["https://openalex.org/W19177013","https://openalex.org/W74998615","https://openalex.org/W155715949","https://openalex.org/W1510613202","https://openalex.org/W1545086492","https://openalex.org/W1546539693","https://openalex.org/W1607384859","https://openalex.org/W1656338766","https://openalex.org/W1827539236","https://openalex.org/W1964483457","https://openalex.org/W1964826325","https://openalex.org/W1965951866","https://openalex.org/W1966199634","https://openalex.org/W1967627896","https://openalex.org/W1968731005","https://openalex.org/W1970168078","https://openalex.org/W1970435434","https://openalex.org/W1970547401","https://openalex.org/W1970562215","https://openalex.org/W1972578057","https://openalex.org/W1972582749","https://openalex.org/W1973328141","https://openalex.org/W1975707478","https://openalex.org/W1975749637","https://openalex.org/W1976139499","https://openalex.org/W1976220825","https://openalex.org/W1976402708","https://openalex.org/W1977479596","https://openalex.org/W1978844022","https://openalex.org/W1979377589","https://openalex.org/W1980882927","https://openalex.org/W1981583319","https://openalex.org/W1982450155","https://openalex.org/W1983030304","https://openalex.org/W1984232298","https://openalex.org/W1986347816","https://openalex.org/W1988219667","https://openalex.org/W1989195046","https://openalex.org/W1990374476","https://openalex.org/W1991473822","https://openalex.org/W1992034168","https://openalex.org/W1993646281","https://openalex.org/W1999259621","https://openalex.org/W2000246597","https://openalex.org/W2000929246","https://openalex.org/W2001942810","https://openalex.org/W2002079983","https://openalex.org/W2002468125","https://openalex.org/W2003516044","https://openalex.org/W2004893394","https://openalex.org/W2007603674","https://openalex.org/W2007861835","https://openalex.org/W2009433201","https://openalex.org/W2009482816","https://openalex.org/W2010685904","https://openalex.org/W2011272082","https://openalex.org/W2011690386","https://openalex.org/W2012073666","https://openalex.org/W2012867962","https://openalex.org/W2014922787","https://openalex.org/W2016976719","https://openalex.org/W2017577963","https://openalex.org/W2018657604","https://openalex.org/W2018710810","https://openalex.org/W2019592377","https://openalex.org/W2020623316","https://openalex.org/W2020871254","https://openalex.org/W2020973468","https://openalex.org/W2021521632","https://openalex.org/W2021534705","https://openalex.org/W2023862860","https://openalex.org/W2023883866","https://openalex.org/W2024208593","https://openalex.org/W2024743028","https://openalex.org/W2025919388","https://openalex.org/W2026934452","https://openalex.org/W2030814766","https://openalex.org/W2031554805","https://openalex.org/W2031918758","https://openalex.org/W2032773633","https://openalex.org/W2033706352","https://openalex.org/W2035408744","https://openalex.org/W2036969572","https://openalex.org/W2037855159","https://openalex.org/W2040594105","https://openalex.org/W2041589924","https://openalex.org/W2041708304","https://openalex.org/W2042319724","https://openalex.org/W2042699875","https://openalex.org/W2043453847","https://openalex.org/W2044136163","https://openalex.org/W2045569977","https://openalex.org/W2045829393","https://openalex.org/W2047006595","https://openalex.org/W2047165821","https://openalex.org/W2047855476","https://openalex.org/W2048393999","https://openalex.org/W2054253522","https://openalex.org/W2054290614","https://openalex.org/W2055758067","https://openalex.org/W2056285452","https://openalex.org/W2056299800","https://openalex.org/W2058013384","https://openalex.org/W2058694919","https://openalex.org/W2059907075","https://openalex.org/W2060194056","https://openalex.org/W2060987284","https://openalex.org/W2061612552","https://openalex.org/W2062764153","https://openalex.org/W2063212792","https://openalex.org/W2064163144","https://openalex.org/W2064548903","https://openalex.org/W2064860928","https://openalex.org/W2064981718","https://openalex.org/W2067848116","https://openalex.org/W2067964115","https://openalex.org/W2069746446","https://openalex.org/W2069928673","https://openalex.org/W2070777173","https://openalex.org/W2071659125","https://openalex.org/W2072359587","https://openalex.org/W2072477221","https://openalex.org/W2075862824","https://openalex.org/W2076664843","https://openalex.org/W2076681068","https://openalex.org/W2077173514","https://openalex.org/W2077228168","https://openalex.org/W2078436717","https://openalex.org/W2078564148","https://openalex.org/W2079529157","https://openalex.org/W2080031538","https://openalex.org/W2081366605","https://openalex.org/W2081577217","https://openalex.org/W2082772537","https://openalex.org/W2083790799","https://openalex.org/W2084871302","https://openalex.org/W2085623438","https://openalex.org/W2086171977","https://openalex.org/W2090002834","https://openalex.org/W2090089216","https://openalex.org/W2093605509","https://openalex.org/W2096088010","https://openalex.org/W2096895785","https://openalex.org/W2096966529","https://openalex.org/W2097112639","https://openalex.org/W2099395117","https://openalex.org/W2099799558","https://openalex.org/W2100647791","https://openalex.org/W2100881236","https://openalex.org/W2102676553","https://openalex.org/W2102845038","https://openalex.org/W2103258118","https://openalex.org/W2108763926","https://openalex.org/W2113545687","https://openalex.org/W2115797520","https://openalex.org/W2118645856","https://openalex.org/W2121149368","https://openalex.org/W2123799923","https://openalex.org/W2123963487","https://openalex.org/W2125753084","https://openalex.org/W2126297234","https://openalex.org/W2126346850","https://openalex.org/W2128106431","https://openalex.org/W2129959630","https://openalex.org/W2131009328","https://openalex.org/W2132919995","https://openalex.org/W2134457997","https://openalex.org/W2134499051","https://openalex.org/W2134670044","https://openalex.org/W2134819724","https://openalex.org/W2135642745","https://openalex.org/W2136395938","https://openalex.org/W2136966549","https://openalex.org/W2137907845","https://openalex.org/W2138436760","https://openalex.org/W2138891766","https://openalex.org/W2139451172","https://openalex.org/W2141862632","https://openalex.org/W2143925916","https://openalex.org/W2145238798","https://openalex.org/W2145342077","https://openalex.org/W2147300476","https://openalex.org/W2148924929","https://openalex.org/W2149934235","https://openalex.org/W2149952617","https://openalex.org/W2150287384","https://openalex.org/W2151354541","https://openalex.org/W2151697521","https://openalex.org/W2152415425","https://openalex.org/W2152732371","https://openalex.org/W2154620120","https://openalex.org/W2155062804","https://openalex.org/W2155976768","https://openalex.org/W2156587383","https://openalex.org/W2156787749","https://openalex.org/W2158407007","https://openalex.org/W2161069579","https://openalex.org/W2164570187","https://openalex.org/W2165918117","https://openalex.org/W2166458266","https://openalex.org/W2166926604","https://openalex.org/W2168663383","https://openalex.org/W2171074560","https://openalex.org/W2183943753","https://openalex.org/W2327867062","https://openalex.org/W2329513153","https://openalex.org/W2329696049","https://openalex.org/W2338517018","https://openalex.org/W2401705493","https://openalex.org/W2408995920","https://openalex.org/W2415013542","https://openalex.org/W2949466761","https://openalex.org/W3023255929","https://openalex.org/W3118072166","https://openalex.org/W3208125024"],"related_works":["https://openalex.org/W2049763771","https://openalex.org/W2026710188","https://openalex.org/W2135576690","https://openalex.org/W7095885020","https://openalex.org/W50706533","https://openalex.org/W7132304140","https://openalex.org/W7095171799","https://openalex.org/W3159378902","https://openalex.org/W2125221090","https://openalex.org/W2037385479"],"abstract_inverted_index":{"Pancreatic":[0],"cancer":[1,47,57,71,169,187,251,275,304],"is":[2],"one":[3],"of":[4,27,30,104,143,161,175,264,268,333],"the":[5,17,39,68,102,119,124,260,281,310,322,329,337],"most":[6],"challenging":[7],"cancers":[8],"to":[9,15,101,130,171,192,214,255],"treat.":[10],"This":[11],"study":[12],"was":[13,277,316],"aimed":[14],"examine":[16],"in":[18,21,45,59,67,138,189,225,245,249,273,302],"vitro":[19,60,190],"and":[20,25,76,86,127,177,221,234,238,270,280,298],"vivo":[22],"anticancer":[23,183,297],"efficacy":[24],"mechanism":[26],"Hsp90":[28,81,227,269,299,319,330],"inhibition":[29,160,248,267,320],"withaferin":[31],"A":[32,50,79,291],"(WA),":[33],"a":[34,139,180,256],"natural":[35],"product":[36],"purified":[37],"from":[38],"Indian":[40],"medicinal":[41],"plant":[42],"Withania":[43],"somnifera,":[44],"pancreatic":[46,56,70,144,168,186,250,274,303],"cells..":[48],"Withaferin":[49,78],"exhibited":[51,179],"potent":[52,153],"antiproliferative":[53],"activity":[54,332],"against":[55,185],"cells":[58,170,188,276],"with":[61,203],"IC50":[62],"values":[63],"below":[64],"3":[65],"microM":[66,205],"three":[69,282],"cell":[72],"lines":[73],"Panc-1,":[74],"MiaPaca-2":[75],"BxPc-3.":[77],"induced":[80,148],"client":[82,228],"protein":[83],"(Akt,":[84],"Cdk4":[85],"glucocorticoid":[87],"receptor)":[88],"degradation":[89],"mediated":[90],"by":[91,111,163,211],"proteasome.":[92],"WA-Biotin":[93],"pull-down":[94],"assay":[95],"showed":[96,242,308],"that":[97,116,309],"WA-biotin":[98],"bound":[99],"directly":[100],"C-terminus":[103],"Hsp90,":[105],"which":[106,151],"could":[107],"be":[108],"competitively":[109],"blocked":[110],"unlabeled":[112],"WA.":[113,172],"Co-immunoprecipitation":[114],"demonstrated":[115],"WA":[117,133,147,178,195,206,215,220,237,279],"disrupted":[118],"Hsp90-Cdc37":[120],"interaction,":[121],"but":[122],"not":[123],"Hsp90-P23":[125],"association":[126],"ATP":[128],"binding":[129],"Hsp90.":[131],"Finally,":[132,259],"significantly":[134],"decreased":[135,207],"tumor":[136,246],"growth":[137,247],"mouse":[140,252],"xenograft":[141],"model":[142],"cancer..":[145],"As":[146],"Hsp70":[149,162,209],"upregulation,":[150],"has":[152],"anti-apoptotic":[154],"activity,":[155],"we":[156],"further":[157],"examined":[158,294],"whether":[159],"myricetin":[164],"(MY)":[165],"would":[166],"sensitize":[167],"Combined":[173,236],"treatment":[174,196,216,240],"MY":[176,193,199,222,239],"much":[181],"higher":[182],"effect":[184],"compared":[191,213,254],"or":[194],"alone.":[197,217],".":[198],"co-treatment":[200],"(10":[201],"microM)":[202],"1":[204],"WA-induced":[208],"expression":[210],"3-fold":[212],"In":[218,335],"addition,":[219],"acted":[223],"synergistically":[224],"downregulating":[226],"proteins,":[229],"including":[230],"mutated":[231],"P53,":[232],"Akt,":[233],"Cdk4.":[235],"also":[241],"significant":[243],"advantage":[244],"xenografts":[253],"single":[257],"treatment.":[258],"structure-activity":[261],"relationship":[262],"(SAR)":[263],"withanolides":[265],"for":[266,295,318],"anti-proliferative":[271],"activities":[272,301],"investigated.":[278],"analogues":[283],"withanolide":[284],"E":[285,288],"(WE),":[286],"4\u03b2-hydroxywithanolide":[287],"(HWE),":[289],"3-aziridinylwithaferin":[290],"(AzWA)":[292],"were":[293],"their":[296],"inhibitory":[300,331],"cells.":[305],"SAR":[306],"analysis":[307],"C-5,":[311],"6":[312],"epoxy":[313],"functional":[314],"group":[315,324],"responsible":[317],"whereas,":[321],"hydroxyl":[323],"at":[325,341],"C-4":[326],"might":[327],"enhance":[328],"withanolide.":[334],"contrast,":[336],"steric":[338],"bulk":[339],"substitution":[340],"C-3":[342],"may":[343],"reduce":[344],"activity.":[345]},"counts_by_year":[{"year":2020,"cited_by_count":1}],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2016-06-24T00:00:00"}