{"id":"https://openalex.org/W46774609","doi":"https://doi.org/10.1055/s-0038-1647037","title":"A Simple Method to Measure Dermatan Sulfate at Sub-Microgram Concentrations in Plasma","display_name":"A Simple Method to Measure Dermatan Sulfate at Sub-Microgram Concentrations in Plasma","publication_year":1988,"publication_date":"1988-01-01","ids":{"openalex":"https://openalex.org/W46774609","doi":"https://doi.org/10.1055/s-0038-1647037","mag":"46774609","pmid":"https://pubmed.ncbi.nlm.nih.gov/3217919"},"language":"en","primary_location":{"id":"doi:10.1055/s-0038-1647037","doi":"https://doi.org/10.1055/s-0038-1647037","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0038-1647037","pdf_url":null,"source":{"id":"https://openalex.org/S206235894","display_name":"Thrombosis and Haemostasis","issn_l":"0340-6245","issn":["0340-6245","2567-689X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thrombosis and Haemostasis","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061839168","display_name":"Dominique Dupouy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210138343","display_name":"H\u00f4pital Purpan","ror":"https://ror.org/03vcx3f97","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I3019448017","https://openalex.org/I4210138343"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"D Dupouy","raw_affiliation_strings":["The Laboratoire d'H\u00e9mostase, Centre de Transfusion Sanguine, H\u00f4pital Purpan, Toulouse, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Laboratoire d'H\u00e9mostase, Centre de Transfusion Sanguine, H\u00f4pital Purpan, Toulouse, France","institution_ids":["https://openalex.org/I4210138343"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075961319","display_name":"Pi\u00e9rre Si\u00e8","orcid":"https://orcid.org/0000-0003-0050-990X","observed_orcids":["https://orcid.org/0000-0003-0050-990X"]},"institutions":[{"id":"https://openalex.org/I4210138343","display_name":"H\u00f4pital Purpan","ror":"https://ror.org/03vcx3f97","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I3019448017","https://openalex.org/I4210138343"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"P Si\u00e9","raw_affiliation_strings":["The Laboratoire d'H\u00e9mostase, Centre de Transfusion Sanguine, H\u00f4pital Purpan, Toulouse, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Laboratoire d'H\u00e9mostase, Centre de Transfusion Sanguine, H\u00f4pital Purpan, Toulouse, France","institution_ids":["https://openalex.org/I4210138343"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111823626","display_name":"F. Dol","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210138343","display_name":"H\u00f4pital Purpan","ror":"https://ror.org/03vcx3f97","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I3019448017","https://openalex.org/I4210138343"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"F Dol","raw_affiliation_strings":["The Laboratoire d'H\u00e9mostase, Centre de Transfusion Sanguine, H\u00f4pital Purpan, Toulouse, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Laboratoire d'H\u00e9mostase, Centre de Transfusion Sanguine, H\u00f4pital Purpan, Toulouse, France","institution_ids":["https://openalex.org/I4210138343"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5001480006","display_name":"B Boneu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210138343","display_name":"H\u00f4pital Purpan","ror":"https://ror.org/03vcx3f97","country_code":"FR","type":"healthcare","lineage":["https://openalex.org/I3019448017","https://openalex.org/I4210138343"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"B Boneu","raw_affiliation_strings":["The Laboratoire d'H\u00e9mostase, Centre de Transfusion Sanguine, H\u00f4pital Purpan, Toulouse, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Laboratoire d'H\u00e9mostase, Centre de Transfusion Sanguine, H\u00f4pital Purpan, Toulouse, France","institution_ids":["https://openalex.org/I4210138343"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210138343"],"apc_list":null,"apc_paid":null,"fwci":0.7366,"has_fulltext":false,"cited_by_count":43,"citation_normalized_percentile":{"value":0.65258597,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":96},"biblio":{"volume":"60","issue":"02","first_page":"236","last_page":"239"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.6381999850273132,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.6381999850273132,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.3499000072479248,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10746","display_name":"Platelet Disorders and Treatments","score":0.002899999963119626,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dermatan-sulfate","display_name":"dermatan sulfate","score":1.0},{"id":"https://openalex.org/keywords/heparin-cofactor-ii","display_name":"heparin cofactor II","score":0.9639999866485596},{"id":"https://openalex.org/keywords/thrombin-inhibition","display_name":"thrombin inhibition","score":0.9629999995231628},{"id":"https://openalex.org/keywords/ionic-strength","display_name":"ionic strength","score":0.6499999761581421},{"id":"https://openalex.org/keywords/heparin","display_name":"heparin","score":0.6589999794960022},{"id":"https://openalex.org/keywords/pentosan-polysulfate-sodium","display_name":"pentosan polysulfate","score":0.9980000257492065},{"id":"https://openalex.org/keywords/glycosaminoglycans","display_name":"glycosaminoglycans","score":0.9649999737739563},{"id":"https://openalex.org/keywords/thrombosis","display_name":"thrombosis","score":0.6850000023841858}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7372627258300781},{"id":"https://openalex.org/C2778066728","wikidata":"https://www.wikidata.org/wiki/Q2335618","display_name":"Dermatan sulfate","level":4,"score":0.725411057472229},{"id":"https://openalex.org/C2777292125","wikidata":"https://www.wikidata.org/wiki/Q409166","display_name":"Thrombin","level":3,"score":0.6746484041213989},{"id":"https://openalex.org/C2779026020","wikidata":"https://www.wikidata.org/wiki/Q416336","display_name":"Antithrombin","level":3,"score":0.655887246131897},{"id":"https://openalex.org/C2778162025","wikidata":"https://www.wikidata.org/wiki/Q14905348","display_name":"Heparin cofactor II","level":4,"score":0.636111855506897},{"id":"https://openalex.org/C187428577","wikidata":"https://www.wikidata.org/wiki/Q898396","display_name":"Ionic strength","level":3,"score":0.5350357890129089},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5073737502098083},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.47208186984062195},{"id":"https://openalex.org/C2777557582","wikidata":"https://www.wikidata.org/wiki/Q190016","display_name":"Heparin","level":2,"score":0.43915629386901855},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2548626661300659},{"id":"https://openalex.org/C2778041096","wikidata":"https://www.wikidata.org/wiki/Q424668","display_name":"Heparan sulfate","level":3,"score":0.20516490936279297},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.1847410798072815},{"id":"https://openalex.org/C89560881","wikidata":"https://www.wikidata.org/wiki/Q101026","display_name":"Platelet","level":2,"score":0.14269980788230896},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.08691981434822083},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0796651542186737},{"id":"https://openalex.org/C184651966","wikidata":"https://www.wikidata.org/wiki/Q906356","display_name":"Aqueous solution","level":2,"score":0.06999948620796204},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000990","descriptor_name":"Antithrombin III","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000991","descriptor_name":"Antithrombins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D001774","descriptor_name":"Blood Chemical Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002807","descriptor_name":"Chondroitin","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D003871","descriptor_name":"Dermatan Sulfate","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D005337","descriptor_name":"Fibrin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007477","descriptor_name":"Ions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015844","descriptor_name":"Heparin Cofactor II","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1055/s-0038-1647037","doi":"https://doi.org/10.1055/s-0038-1647037","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0038-1647037","pdf_url":null,"source":{"id":"https://openalex.org/S206235894","display_name":"Thrombosis and Haemostasis","issn_l":"0340-6245","issn":["0340-6245","2567-689X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thrombosis and Haemostasis","raw_type":"journal-article"},{"id":"pmid:3217919","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3217919","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thrombosis and haemostasis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2002017476","https://openalex.org/W2413106846","https://openalex.org/W1560801910","https://openalex.org/W2005929205","https://openalex.org/W1998568483","https://openalex.org/W2429081025","https://openalex.org/W2039389052","https://openalex.org/W1972977849","https://openalex.org/W2038576830","https://openalex.org/W2443769547"],"abstract_inverted_index":{"A":[0],"simple":[1],"method":[2,55],"for":[3,98],"biological":[4],"assay":[5,28,159,184],"of":[6,26,41,59,66,69,74,82,147,153,214,222,224,236],"dermatan":[7],"sulfate":[8],"(DS)":[9],"in":[10,46,121,137,164,177,202,216,231],"plasma":[11,48,138,217],"is":[12,29,130,155,185,200],"described.":[13],"DS":[14,67,135,176,199,215,237],"accelerates":[15],"thrombin":[16,35,75,111,149],"inhibition":[17,76],"by":[18,88],"heparin":[19,195],"cofactor":[20],"II":[21,45,96,116,191],"(HC":[22],"II).":[23],"The":[24,151,158,183],"principle":[25],"the":[27,32,72,79,83,105,126,145,220],"to":[30,141,174,188],"measure":[31,175],"residual":[33,148],"amidolytic":[34],"activity":[36],"after":[37],"a":[38,99,119,122,131],"short":[39],"period":[40],"incubation":[42],"with":[43,114],"HC":[44,70,95,115,190],"defibrinated":[47],"at":[49,63],"low":[50],"ionic":[51,80],"strength.":[52],"For":[53],"this":[54,225],"we":[56],"take":[57],"advantage":[58],"two":[60],"observations.":[61],"Firstly,":[62],"fixed":[64],"concentrations":[65,136,213],"and":[68,97,144,167,180,196,238],"II,":[71],"rate":[73],"increases":[77],"when":[78],"strength":[81],"medium":[84],"decreases.":[85],"Secondly,":[86],"defibrination":[87],"bentonite":[89],"absorption":[90],"also":[91,186],"removes":[92],"antithrombin":[93],"III,":[94],"large":[100],"part":[101],"alpha-2":[102],"macroglobulin":[103],"from":[104,139],"plasma,":[106],"so":[107],"that":[108],"no":[109],"other":[110,189],"inhibitor":[112],"competes":[113],"added":[117],"as":[118,194],"reagent":[120],"second":[123],"step.":[124],"In":[125],"conditions":[127],"described,":[128],"there":[129],"linear":[132],"relationship":[133],"between":[134],"0":[140],"2":[142],"micrograms/ml":[143],"log":[146],"activity.":[150],"limit":[152],"sensitivity":[154,223],"0.1":[156],"micrograms/ml.":[157],"displays":[160],"an":[161],"acceptable":[162],"reproducibility":[163],"intra-assay,":[165],"inter-assay":[166],"inter-individual":[168],"experiments.":[169],"It":[170],"can":[171],"be":[172,229],"used":[173],"human,":[178],"rabbit":[179],"rat":[181],"plasmas.":[182],"sensitive":[187],"activators":[192],"such":[193],"pentosan":[197],"polysulfate.":[198],"effective":[201],"experimental":[203,232],"thrombosis":[204],"without":[205],"any":[206],"detectable":[207],"anticoagulant":[208],"effect":[209],"ex":[210],"vivo.":[211],"Pharmacological":[212],"fall":[218],"into":[219],"range":[221],"assay,":[226],"which":[227],"would":[228],"helpful":[230],"or":[233],"clinical":[234],"studies":[235],"related":[239],"glycosaminoglycans.":[240]},"counts_by_year":[{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}