{"id":"https://openalex.org/W45809337","doi":"https://doi.org/10.1007/978-1-59745-352-3_2","title":"Isolation and Primary Culture of Human Proximal Tubule Cells","display_name":"Isolation and Primary Culture of Human Proximal Tubule Cells","publication_year":2008,"publication_date":"2008-10-03","ids":{"openalex":"https://openalex.org/W45809337","doi":"https://doi.org/10.1007/978-1-59745-352-3_2","mag":"45809337","pmid":"https://pubmed.ncbi.nlm.nih.gov/19148604"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-59745-352-3_2","doi":"https://doi.org/10.1007/978-1-59745-352-3_2","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-352-3_2","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4236068561","display_name":"Kidney Research"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045147978","display_name":"David Alan Vesey","orcid":"https://orcid.org/0000-0002-2856-6650","observed_orcids":["https://orcid.org/0000-0002-2856-6650"]},"institutions":[{"id":"https://openalex.org/I165143802","display_name":"The University of Queensland","ror":"https://ror.org/00rqy9422","country_code":"AU","type":"education","lineage":["https://openalex.org/I165143802"]},{"id":"https://openalex.org/I2801814493","display_name":"Princess Alexandra Hospital","ror":"https://ror.org/04mqb0968","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I1302211766","https://openalex.org/I2801244131","https://openalex.org/I2801814493","https://openalex.org/I4210089726"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"David A. Vesey","raw_affiliation_strings":["Centre for Kidney Disease Research, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia","University of Queensland Princess Alexandra Hospital"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Kidney Disease Research, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, Queensland, Australia","institution_ids":["https://openalex.org/I165143802","https://openalex.org/I2801814493"]},{"raw_affiliation_string":"University of Queensland Princess Alexandra Hospital","institution_ids":["https://openalex.org/I165143802","https://openalex.org/I2801814493"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100868205","display_name":"Weier Qi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]},{"id":"https://openalex.org/I2800079252","display_name":"Royal North Shore Hospital","ror":"https://ror.org/02gs2e959","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2800079252"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Weier Qi","raw_affiliation_strings":["Department of Medicine, University of Sydney, Kolling Institute, Royal North Shore Hospital, Sydney, New South Wales, Australia","University of Sydney, Kolling Institute, Royal North Shore Hospital"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Sydney, Kolling Institute, Royal North Shore Hospital, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602","https://openalex.org/I2800079252"]},{"raw_affiliation_string":"University of Sydney, Kolling Institute, Royal North Shore Hospital","institution_ids":["https://openalex.org/I129604602","https://openalex.org/I2800079252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101543871","display_name":"Xinming Chen","orcid":"https://orcid.org/0000-0003-3212-7172","observed_orcids":["https://orcid.org/0000-0003-3212-7172"]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]},{"id":"https://openalex.org/I2800079252","display_name":"Royal North Shore Hospital","ror":"https://ror.org/02gs2e959","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2800079252"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Xinming Chen","raw_affiliation_strings":["Department of Medicine, University of Sydney, Kolling Institute, Royal North Shore Hospital, Sydney, New South Wales, Australia","University of Sydney, Kolling Institute, Royal North Shore Hospital"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Sydney, Kolling Institute, Royal North Shore Hospital, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602","https://openalex.org/I2800079252"]},{"raw_affiliation_string":"University of Sydney, Kolling Institute, Royal North Shore Hospital","institution_ids":["https://openalex.org/I129604602","https://openalex.org/I2800079252"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016584401","display_name":"Carol A. Pollock","orcid":"https://orcid.org/0000-0001-7167-6495","observed_orcids":["https://orcid.org/0000-0001-7167-6495"]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]},{"id":"https://openalex.org/I2800079252","display_name":"Royal North Shore Hospital","ror":"https://ror.org/02gs2e959","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2800079252"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Carol A. Pollock","raw_affiliation_strings":["Department of Medicine, University of Sydney, Kolling Institute, Royal North Shore Hospital, Sydney, New South Wales, Australia","University of Sydney, Kolling Institute, Royal North Shore Hospital"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Sydney, Kolling Institute, Royal North Shore Hospital, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602","https://openalex.org/I2800079252"]},{"raw_affiliation_string":"University of Sydney, Kolling Institute, Royal North Shore Hospital","institution_ids":["https://openalex.org/I129604602","https://openalex.org/I2800079252"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5043394711","display_name":"David Wayne Johnson","orcid":"https://orcid.org/0000-0001-5491-3460","observed_orcids":["https://orcid.org/0000-0001-5491-3460","https://orcid.org/0000-0001-8453-7849","https://orcid.org/0000-0001-6932-2460"]},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]},{"id":"https://openalex.org/I2800079252","display_name":"Royal North Shore Hospital","ror":"https://ror.org/02gs2e959","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2800079252"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"David W. Johnson","raw_affiliation_strings":["Department of Medicine, University of Sydney, Kolling Institute, Royal North Shore Hospital, Sydney, New South Wales, Australia","University of Sydney, Kolling Institute, Royal North Shore Hospital"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, University of Sydney, Kolling Institute, Royal North Shore Hospital, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602","https://openalex.org/I2800079252"]},{"raw_affiliation_string":"University of Sydney, Kolling Institute, Royal North Shore Hospital","institution_ids":["https://openalex.org/I129604602","https://openalex.org/I2800079252"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":6.2977,"has_fulltext":false,"cited_by_count":39,"citation_normalized_percentile":{"value":0.9538835,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"466","issue":null,"first_page":"19","last_page":"24"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10684","display_name":"Acute Kidney Injury Research","score":0.29420000314712524,"subfield":{"id":"https://openalex.org/subfields/2727","display_name":"Nephrology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10684","display_name":"Acute Kidney Injury Research","score":0.29420000314712524,"subfield":{"id":"https://openalex.org/subfields/2727","display_name":"Nephrology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11029","display_name":"Renal Diseases and Glomerulopathies","score":0.26420000195503235,"subfield":{"id":"https://openalex.org/subfields/2727","display_name":"Nephrology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.1632000058889389,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/proximal-tubular-epithelial-cells","display_name":"proximal tubular epithelial cells","score":0.8769999742507935},{"id":"https://openalex.org/keywords/primary-culture","display_name":"primary cell culture","score":0.9129999876022339},{"id":"https://openalex.org/keywords/cell-isolation","display_name":"cell isolation","score":0.7360000014305115},{"id":"https://openalex.org/keywords/percoll-gradient","display_name":"Percoll density gradient","score":0.8119999766349792},{"id":"https://openalex.org/keywords/collagenase-digestion","display_name":"collagenase digestion","score":0.9890000224113464},{"id":"https://openalex.org/keywords/renal-cortex","display_name":"renal cortex","score":0.7160000205039978},{"id":"https://openalex.org/keywords/cell-monolayers","display_name":"cell monolayers","score":0.6320000290870667},{"id":"https://openalex.org/keywords/human-kidney","display_name":"human kidney","score":0.492000013589859}],"concepts":[{"id":"https://openalex.org/C48679268","wikidata":"https://www.wikidata.org/wiki/Q3375169","display_name":"Percoll","level":3,"score":0.8405193090438843},{"id":"https://openalex.org/C2780498532","wikidata":"https://www.wikidata.org/wiki/Q7850941","display_name":"Tubule","level":3,"score":0.6837233304977417},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5151382684707642},{"id":"https://openalex.org/C2778394429","wikidata":"https://www.wikidata.org/wiki/Q304956","display_name":"Collagenase","level":3,"score":0.4725724756717682},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.46397385001182556},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.46056610345840454},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.45261555910110474},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4509144425392151},{"id":"https://openalex.org/C2780222010","wikidata":"https://www.wikidata.org/wiki/Q2916078","display_name":"Proximal tubule","level":3,"score":0.442050039768219},{"id":"https://openalex.org/C2780091579","wikidata":"https://www.wikidata.org/wiki/Q9377","display_name":"Kidney","level":2,"score":0.41972485184669495},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3737128674983978},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.16344258189201355},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.1613876223564148},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1381479799747467},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10502466559410095},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09028014540672302},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007687","descriptor_name":"Kidney Tubules, Proximal","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-59745-352-3_2","doi":"https://doi.org/10.1007/978-1-59745-352-3_2","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-352-3_2","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:19148604","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19148604","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8048999905586243}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/15","display_name":"Life on land","score":0.47999998927116394}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334705","display_name":"National Health and Medical Research Council","ror":"https://ror.org/011kf5r70"},{"id":"https://openalex.org/F4320334737","display_name":"National Medical Research Council","ror":"https://ror.org/04x3cxs03"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W85899443","https://openalex.org/W1986569619","https://openalex.org/W1990745136","https://openalex.org/W2057683674","https://openalex.org/W2078790282","https://openalex.org/W2122668255","https://openalex.org/W2125917822","https://openalex.org/W2235475950","https://openalex.org/W2335420238","https://openalex.org/W2410719978","https://openalex.org/W2443501177"],"related_works":["https://openalex.org/W2085631433","https://openalex.org/W2411081953","https://openalex.org/W2377616817","https://openalex.org/W2054310798","https://openalex.org/W2387842933","https://openalex.org/W2369163463","https://openalex.org/W2966024039","https://openalex.org/W1980951794","https://openalex.org/W1983095757","https://openalex.org/W161078271"],"abstract_inverted_index":{"Primary":[0],"cultures":[1],"of":[2,25,58,76,111,120,142],"renal":[3,26],"proximal":[4],"tubule":[5,14,112],"cells":[6,94],"(PTC)":[7],"have":[8],"been":[9],"widely":[10],"used":[11,66],"to":[12,39,70,146],"investigate":[13],"cell":[15,28,49],"function.":[16],"They":[17],"provide":[18],"a":[19,64,98,103,115,125,133],"model":[20],"system":[21],"where":[22],"confounding":[23],"influences":[24],"haemodynamics,":[27],"heterogeneity,":[29],"and":[30,56,72,87,100],"neural":[31],"activity":[32],"are":[33,37,52,57,95],"eliminated.":[34],"Additionally":[35],"they":[36,131],"likely":[38],"more":[40],"closely":[41],"resemble":[42],"PTC":[43,143],"in":[44,67,124],"vivo":[45],"than":[46],"established":[47],"kidney":[48],"lines,":[50],"which":[51],"often":[53],"virally":[54],"immortalised":[55],"uncertain":[59],"origin.":[60],"This":[61],"chapter":[62],"describes":[63],"method":[65],"our":[68],"laboratories":[69],"isolate":[71],"culture":[73],"pure":[74,109],"populations":[75],"human":[77],"PTC.":[78],"The":[79],"cortex":[80],"is":[81],"dissected":[82],"away":[83],"from":[84],"the":[85,93,118,121,139],"medulla":[86],"minced":[88],"finely.":[89],"Following":[90],"collagenase":[91],"digestion,":[92],"passed":[96],"through":[97],"sieve":[99],"separated":[101],"on":[102],"Percoll":[104],"density":[105],"gradient.":[106,122],"An":[107],"almost":[108],"population":[110],"fragments":[113],"form":[114,132],"band":[116],"at":[117],"base":[119],"Cultured":[123],"hormonally":[126],"defined":[127],"serum-free":[128],"growth":[129],"media,":[130],"tightly":[134],"packed":[135],"monolayer":[136],"that":[137],"retains":[138],"differentiated":[140],"characteristics":[141],"for":[144],"up":[145],"three":[147],"passages.":[148]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":6},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}