{"id":"https://openalex.org/W45547304","doi":null,"title":"DEVELOPMENT OF PRIMARY NEURONAL CULTURE OF EMBRYONIC RABBIT DORSAL ROOT GANGLIA FOR MICROFLUIDIC CHAMBER ANALYSIS OF AXON MEDIATED NEURONAL SPREAD OF BOVINE HERPESVIRUS TYPE 1.","display_name":"DEVELOPMENT OF PRIMARY NEURONAL CULTURE OF EMBRYONIC RABBIT DORSAL ROOT GANGLIA FOR MICROFLUIDIC CHAMBER ANALYSIS OF AXON MEDIATED NEURONAL SPREAD OF BOVINE HERPESVIRUS TYPE 1.","publication_year":2010,"publication_date":"2010-01-01","ids":{"openalex":"https://openalex.org/W45547304","mag":"45547304"},"language":"en","primary_location":{"id":"pmh:oai:krex.k-state.edu:2097/4115","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2097/4115","pdf_url":"http://hdl.handle.net/2097/4115","source":{"id":"https://openalex.org/S4306402339","display_name":"K-State Research Exchange (Kansas State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I189590672","host_organization_name":"Kansas State University","host_organization_lineage":["https://openalex.org/I189590672"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/2097/4115","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5018541296","display_name":"C. Coats","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"C. Coats","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5018541296"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10625","display_name":"Herpesvirus Infections and Treatments","score":0.9824000000953674,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10625","display_name":"Herpesvirus Infections and Treatments","score":0.9824000000953674,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.009399999864399433,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bovine-herpesvirus-1","display_name":"bovine herpesvirus 1","score":0.9779999852180481},{"id":"https://openalex.org/keywords/dorsal-root-ganglia","display_name":"dorsal root ganglia","score":0.902999997138977},{"id":"https://openalex.org/keywords/primary-neuronal-cultures","display_name":"primary neuronal cultures","score":0.8500000238418579},{"id":"https://openalex.org/keywords/microfluidic-chamber","display_name":"microfluidic chamber","score":0.9210000038146973},{"id":"https://openalex.org/keywords/infectious-bovine-rhinotracheitis","display_name":"infectious bovine rhinotracheitis","score":0.9120000004768372},{"id":"https://openalex.org/keywords/rabbit-embryo","display_name":"rabbit embryo","score":0.38600000739097595},{"id":"https://openalex.org/keywords/axonal-transport","display_name":"axonal transport","score":0.6039999723434448},{"id":"https://openalex.org/keywords/live-cell-imaging","display_name":"live-cell imaging","score":0.5989999771118164}],"concepts":[{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.5926060676574707},{"id":"https://openalex.org/C2779884254","wikidata":"https://www.wikidata.org/wiki/Q492162","display_name":"Rabbit (cipher)","level":2,"score":0.5481548309326172},{"id":"https://openalex.org/C2779530196","wikidata":"https://www.wikidata.org/wiki/Q178999","display_name":"Axon","level":2,"score":0.5471134185791016},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5437851548194885},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.509339451789856},{"id":"https://openalex.org/C140530291","wikidata":"https://www.wikidata.org/wiki/Q1225063","display_name":"Dorsum","level":2,"score":0.503678023815155},{"id":"https://openalex.org/C2994367422","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Primary culture","level":3,"score":0.4897494316101074},{"id":"https://openalex.org/C69125201","wikidata":"https://www.wikidata.org/wiki/Q2034716","display_name":"Axon guidance","level":3,"score":0.4525132477283478},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.44194042682647705},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.3355105221271515},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.19115585088729858},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07776206731796265},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.05827540159225464},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C38652104","wikidata":"https://www.wikidata.org/wiki/Q3510521","display_name":"Computer security","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:krex.k-state.edu:2097/4115","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2097/4115","pdf_url":"http://hdl.handle.net/2097/4115","source":{"id":"https://openalex.org/S4306402339","display_name":"K-State Research Exchange (Kansas State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I189590672","host_organization_name":"Kansas State University","host_organization_lineage":["https://openalex.org/I189590672"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:45547304","doi":null,"is_oa":false,"landing_page_url":"https://krex.k-state.edu/dspace/handle/2097/4115","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:krex.k-state.edu:2097/4115","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2097/4115","pdf_url":"http://hdl.handle.net/2097/4115","source":{"id":"https://openalex.org/S4306402339","display_name":"K-State Research Exchange (Kansas State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I189590672","host_organization_name":"Kansas State University","host_organization_lineage":["https://openalex.org/I189590672"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W45547304.pdf","grobid_xml":"https://content.openalex.org/works/W45547304.grobid-xml"},"referenced_works_count":74,"referenced_works":["https://openalex.org/W24286860","https://openalex.org/W120643878","https://openalex.org/W201594725","https://openalex.org/W1513464990","https://openalex.org/W1546841203","https://openalex.org/W1581748227","https://openalex.org/W1851549849","https://openalex.org/W1906146321","https://openalex.org/W1917226481","https://openalex.org/W1934714969","https://openalex.org/W1943430075","https://openalex.org/W1949837426","https://openalex.org/W1967437498","https://openalex.org/W1970182993","https://openalex.org/W1970245110","https://openalex.org/W1974604937","https://openalex.org/W1980548298","https://openalex.org/W1981791822","https://openalex.org/W1990538398","https://openalex.org/W1999772832","https://openalex.org/W2021851305","https://openalex.org/W2030434282","https://openalex.org/W2032311916","https://openalex.org/W2033117797","https://openalex.org/W2036564583","https://openalex.org/W2037242552","https://openalex.org/W2038784718","https://openalex.org/W2043549364","https://openalex.org/W2043749168","https://openalex.org/W2044386529","https://openalex.org/W2048451279","https://openalex.org/W2051414005","https://openalex.org/W2051853081","https://openalex.org/W2061272721","https://openalex.org/W2066005663","https://openalex.org/W2072565410","https://openalex.org/W2073284464","https://openalex.org/W2078782272","https://openalex.org/W2078837666","https://openalex.org/W2082631326","https://openalex.org/W2086341057","https://openalex.org/W2088339137","https://openalex.org/W2092373729","https://openalex.org/W2093310901","https://openalex.org/W2099382752","https://openalex.org/W2101269168","https://openalex.org/W2107971958","https://openalex.org/W2109244138","https://openalex.org/W2118600936","https://openalex.org/W2119336074","https://openalex.org/W2121232238","https://openalex.org/W2122380105","https://openalex.org/W2123193533","https://openalex.org/W2127117847","https://openalex.org/W2130154563","https://openalex.org/W2132223883","https://openalex.org/W2135039865","https://openalex.org/W2138002961","https://openalex.org/W2139021209","https://openalex.org/W2141363673","https://openalex.org/W2142404794","https://openalex.org/W2143226256","https://openalex.org/W2152786021","https://openalex.org/W2157275850","https://openalex.org/W2159921922","https://openalex.org/W2163837394","https://openalex.org/W2164447047","https://openalex.org/W2165864728","https://openalex.org/W2167040184","https://openalex.org/W2168364438","https://openalex.org/W2169191388","https://openalex.org/W2170701797","https://openalex.org/W2171667802","https://openalex.org/W2409323839"],"related_works":["https://openalex.org/W4292589547","https://openalex.org/W2398333835","https://openalex.org/W4396219718","https://openalex.org/W2366872661","https://openalex.org/W2742336540","https://openalex.org/W4401236608","https://openalex.org/W4254766791","https://openalex.org/W2205104871","https://openalex.org/W2368281601","https://openalex.org/W2560708291"],"abstract_inverted_index":{"Bovine":[0,35],"herpesvirus":[1],"type":[2,230],"1":[3],"(BHV-1)":[4],"is":[5,29],"an":[6,30],"important":[7,31],"pathogen":[8],"of":[9,33,43,64,70,115,128,148,162,188],"cattle":[10,66,78],"that":[11,211],"can":[12],"cause":[13],"severe":[14],"respiratory":[15],"tract":[16],"infection":[17,163],"known":[18],"as":[19,164,166],"infectious":[20],"bovine":[21],"rhinotracheitis":[22],"(IBR),":[23],"abortion":[24],"in":[25,52,58,73,94,101,139,169,191],"pregnant":[26],"cows,":[27],"and":[28,61,76,81,104,143,180,183,198,215],"component":[32],"the":[34,59,68,71,95,102,112,123,140,151,184,202],"Respiratory":[36],"Disease":[37],"Complex":[38],"(BRDC,":[39],"\"Shipping":[40],"fever\").The":[41],"ability":[42],"BHV-1":[44,178,219,228],"to":[45,55,98,136,156,177,227],"transport":[46,168,225],"anterogradely":[47],"from":[48,91],"neuron":[49,206],"cell":[50,92,196],"bodies":[51,93],"trigeminal":[53,96],"ganglia":[54,97,126],"axon":[56,158],"termini":[57,100],"nasal":[60],"ocular":[62],"epithelia":[63],"infected":[65,77],"complicates":[67],"control":[69],"disease":[72],"both":[74],"vaccinated":[75],"populations.In":[79],"calves":[80],"rabbits,":[82],"Us9":[83,212,216,232],"deleted":[84],"viruses":[85,220],"have":[86],"defective":[87,222],"anterograde":[88,223],"neuronal":[89,113,120,160,173,224],"spread":[90,108,114,161,190],"nerve":[99],"nose":[103],"eye":[105],"but":[106],"retrograde":[107],"remains":[109],"unaffected.To":[110],"characterize":[111],"BHV-1,":[116],"we":[117,209],"developed":[118],"primary":[119],"cultures":[121,193],"using":[122,194,201],"dorsal":[124],"root":[125],"(DRG)":[127],"rabbit":[129,171],"embryos.We":[130],"successfully":[131],"used":[132],"microfluidic":[133,203],"chamber":[134,192,204],"devices":[135],"isolate":[137],"DRG":[138,172],"somal":[141],"compartment":[142],"allowed":[144,186],"for":[145],"efficient":[146],"growth":[147],"axons":[149],"into":[150],"axonal":[152],"compartment.This":[153],"enabled":[154],"us":[155],"study":[157],"mediated":[159],"well":[165],"viral":[167,189],"axons.Thus,":[170],"culture":[174,207],"was":[175],"susceptible":[176],"mutant":[179,218],"wild-type":[181],"infection,":[182],"method":[185],"visualization":[187],"live":[195],"imaging":[197],"fluorescent":[199],"microscopy.Lastly,":[200],"compartmentalized":[205],"system":[208],"showed":[210],"acidic":[213],"domain-deleted":[214],"null":[217],"had":[221],"relative":[226],"wild":[229],"and/or":[231],"rescued":[233],"viruses.":[234]},"counts_by_year":[{"year":2017,"cited_by_count":1}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}
