{"id":"https://openalex.org/W4416880482","doi":"https://doi.org/10.1681/asn.2025046s42fx","title":"Multimodal Spatial Imaging Reveals Endogenous Adenine as a Metabolic Inducer of Senescence in Aging Kidneys","display_name":"Multimodal Spatial Imaging Reveals Endogenous Adenine as a Metabolic Inducer of Senescence in Aging Kidneys","publication_year":2025,"publication_date":"2025-10-01","ids":{"openalex":"https://openalex.org/W4416880482","doi":"https://doi.org/10.1681/asn.2025046s42fx"},"language":"en","primary_location":{"id":"doi:10.1681/asn.2025046s42fx","doi":"https://doi.org/10.1681/asn.2025046s42fx","is_oa":false,"landing_page_url":"https://doi.org/10.1681/asn.2025046s42fx","pdf_url":null,"source":{"id":"https://openalex.org/S115693375","display_name":"Journal of the American Society of Nephrology","issn_l":"1046-6673","issn":["1046-6673","1533-3450"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315620","host_organization_name":"American Society of Nephrology","host_organization_lineage":["https://openalex.org/P4310315620"],"host_organization_lineage_names":["American Society of Nephrology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society of Nephrology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014467800","display_name":"Soumya Maity","orcid":"https://orcid.org/0000-0002-3407-1734","observed_orcids":["https://orcid.org/0000-0002-3407-1734"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Soumya Maity","raw_affiliation_strings":["The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108143837","display_name":"Afaf Saliba","orcid":"https://orcid.org/0000-0003-2576-4069","observed_orcids":["https://orcid.org/0000-0003-2576-4069"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Afaf Saliba","raw_affiliation_strings":["The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101672859","display_name":"Hak Joo Lee","orcid":"https://orcid.org/0000-0003-3954-3216","observed_orcids":["https://orcid.org/0000-0003-3954-3216"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hak Joo Lee","raw_affiliation_strings":["The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102725800","display_name":"Ian M. Tamayo","orcid":"https://orcid.org/0009-0009-1521-2915","observed_orcids":["https://orcid.org/0009-0009-1521-2915"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ian M. Tamayo","raw_affiliation_strings":["The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111154486","display_name":"Alan Shaji","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alan Shaji","raw_affiliation_strings":["The University of Texas at Austin, Austin, Texas, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas at Austin, Austin, Texas, United States","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071634737","display_name":"Guanshi Zhang","orcid":"https://orcid.org/0000-0001-6557-4521","observed_orcids":["https://orcid.org/0000-0001-6557-4521"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Guanshi Zhang","raw_affiliation_strings":["The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045990646","display_name":"Adam B. Salmon","orcid":"https://orcid.org/0000-0002-1475-7843","observed_orcids":["https://orcid.org/0000-0002-1475-7843"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Adam Salmon","raw_affiliation_strings":["The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088903449","display_name":"Kumar Sharma","orcid":"https://orcid.org/0000-0002-7550-8525","observed_orcids":["https://orcid.org/0000-0002-7550-8525"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kumar Sharma","raw_affiliation_strings":["The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States;","institution_ids":["https://openalex.org/I165951966"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.2877,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.39587791,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":91,"max":95},"biblio":{"volume":"36","issue":"10S","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10937","display_name":"Telomeres, Telomerase, and Senescence","score":0.6542999744415283,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10937","display_name":"Telomeres, Telomerase, and Senescence","score":0.6542999744415283,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.12080000340938568,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10676","display_name":"Genetics, Aging, and Longevity in Model Organisms","score":0.08089999854564667,"subfield":{"id":"https://openalex.org/subfields/1302","display_name":"Aging"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cellular-senescence","display_name":"cellular senescence","score":0.9850000143051147},{"id":"https://openalex.org/keywords/kidney-aging","display_name":"kidney aging","score":0.7549999952316284},{"id":"https://openalex.org/keywords/chronic-kidney-disease","display_name":"chronic kidney disease","score":0.9380000233650208},{"id":"https://openalex.org/keywords/senescence-associated-secretory-phenotype","display_name":"senescence-associated secretory phenotype","score":0.949999988079071},{"id":"https://openalex.org/keywords/p16","display_name":"p16","score":0.6299999952316284},{"id":"https://openalex.org/keywords/p21","display_name":"p21","score":0.781000018119812},{"id":"https://openalex.org/keywords/mtorc1","display_name":"mTORC1","score":0.7749999761581421},{"id":"https://openalex.org/keywords/ischemia-reperfusion-injury","display_name":"ischemia-reperfusion injury","score":0.8790000081062317},{"id":"https://openalex.org/keywords/cisplatin-induced-acute-kidney-injury","display_name":"cisplatin-induced acute kidney injury","score":0.9580000042915344},{"id":"https://openalex.org/keywords/spatial-metabolomics","display_name":"spatial metabolomics","score":0.7509999871253967},{"id":"https://openalex.org/keywords/maldi-mass-spectrometry-imaging","display_name":"MALDI mass spectrometry imaging","score":0.7979999780654907},{"id":"https://openalex.org/keywords/multimodal-imaging","display_name":"multimodal imaging","score":0.7490000128746033}],"concepts":[{"id":"https://openalex.org/C522857546","wikidata":"https://www.wikidata.org/wiki/Q2070979","display_name":"Senescence","level":2,"score":0.8253999948501587},{"id":"https://openalex.org/C16613235","wikidata":"https://www.wikidata.org/wiki/Q285982","display_name":"Endogeny","level":2,"score":0.7822999954223633},{"id":"https://openalex.org/C2225953","wikidata":"https://www.wikidata.org/wiki/Q1661899","display_name":"Inducer","level":3,"score":0.734499990940094},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5478000044822693},{"id":"https://openalex.org/C2779523775","wikidata":"https://www.wikidata.org/wiki/Q9075999","display_name":"Cellular senescence","level":4,"score":0.5340999960899353},{"id":"https://openalex.org/C2780091579","wikidata":"https://www.wikidata.org/wiki/Q9377","display_name":"Kidney","level":2,"score":0.4756999909877777},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4453999996185303},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36899998784065247},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2924000024795532},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2906000018119812},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.2709999978542328},{"id":"https://openalex.org/C500499127","wikidata":"https://www.wikidata.org/wiki/Q332154","display_name":"Ageing","level":2,"score":0.257999986410141},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.25519999861717224}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1681/asn.2025046s42fx","doi":"https://doi.org/10.1681/asn.2025046s42fx","is_oa":false,"landing_page_url":"https://doi.org/10.1681/asn.2025046s42fx","pdf_url":null,"source":{"id":"https://openalex.org/S115693375","display_name":"Journal of the American Society of Nephrology","issn_l":"1046-6673","issn":["1046-6673","1533-3450"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315620","host_organization_name":"American Society of Nephrology","host_organization_lineage":["https://openalex.org/P4310315620"],"host_organization_lineage_names":["American Society of Nephrology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society of Nephrology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9537000060081482,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4295123530","https://openalex.org/W4397045233","https://openalex.org/W7133433594","https://openalex.org/W7140323727","https://openalex.org/W7207635961","https://openalex.org/W4411875730","https://openalex.org/W4210452950","https://openalex.org/W3197580024","https://openalex.org/W7133412450","https://openalex.org/W4399332193"],"abstract_inverted_index":{"Background:":[0],"Cellular":[1],"senescence":[2,229,274],"is":[3],"a":[4,269,285],"key":[5],"contributor":[6],"to":[7,49,60,129,205,222,235,289],"age-related":[8],"kidney":[9,67,102,180],"dysfunction,":[10],"yet":[11],"its":[12,281],"underlying":[13],"mechanisms":[14],"remain":[15],"poorly":[16],"understood.":[17],"Senescent":[18],"cells":[19,56,81,89],"are":[20],"characterized":[21],"by":[22],"p53":[23],"activation,":[24],"accumulation":[25,168,233],"of":[26,54,65,87,123,134,148,158,211,244,272],"cyclin-dependent":[27],"kinase":[28],"inhibitors":[29],"(p21":[30],"and":[31,37,108,126,198,214,251,257,277],"p16),":[32],"elevated":[33],"\u03b2-galactosidase":[34],"(\u03b2-gal)":[35],"activity,":[36],"senescence-associated":[38],"secretory":[39],"phenotype":[40],"(SASP).":[41],"Given":[42],"their":[43],"distinct":[44],"metabolic":[45,63,132,270],"profile,":[46],"we":[47,118],"aimed":[48],"characterize":[50],"the":[51,131,138,149],"metabolomic":[52],"signatures":[53],"senescent":[55,88,135,156,171],"in":[57,70,78,90,137,155,169,179,254,275],"aged":[58],"kidneys":[59,159,220,237],"identify":[61,265],"novel":[62,286],"drivers":[64],"chronic":[66],"disease":[68],"(CKD)":[69],"aging.":[71,298],"Methods:":[72],"We":[73],"used":[74],"p16-tdTomato":[75,162],"reporter":[76,196],"mice,":[77,106,197,219],"which":[79],"p16-expressing":[80],"fluoresce":[82],"red,":[83],"enabling":[84],"precise":[85],"localization":[86],"situ.":[91],"Matrix-assisted":[92],"laser":[93],"desorption/ionization":[94],"mass":[95],"spectrometry":[96],"imaging":[97,165],"(MALDI-MSI)":[98],"was":[99],"performed":[100],"on":[101],"tissues":[103],"from":[104,160,183],"these":[105],"marmosets,":[107],"KPMP":[109],"participants.":[110],"By":[111],"integrating":[112],"spatial":[113,142],"metabolomics":[114,143],"with":[115,230,294],"optical":[116],"imaging,":[117],"generated":[119],"high-resolution":[120],"multimodal":[121],"maps":[122],"tissue":[124],"architecture":[125],"metabolite":[127],"distribution":[128],"profile":[130],"landscape":[133],"regions":[136,157],"kidneys.":[139,279],"Results:":[140],"Untargeted":[141],"identified":[144],"adenine":[145,167,174,232,246,267],"as":[146,268],"one":[147,238],"top":[150],"four":[151],"altered":[152],"small-molecule":[153],"metabolites":[154],"15-month-old":[161,193],"mice.":[163],"Multimodal":[164],"revealed":[166],"\u03b2-gal-positive":[170],"regions.":[172],"Elevated":[173],"levels":[175],"were":[176],"also":[177],"observed":[178],"cortex":[181],"samples":[182],"older":[184],"(>60":[185],"years)":[186,190],"vs.":[187,194,200],"younger":[188],"(<40":[189],"human":[191],"donors,":[192],"3-month-old":[195],"16-year-old":[199],"3-year-old":[201],"marmosets.":[202],"Adenine":[203],"administration":[204],"young":[206],"mice":[207],"induced":[208],"mTORC1-mediated":[209],"expression":[210],"p21,":[212],"p16,":[213],"SASP":[215,253],"markers.":[216],"In":[217],"p16-reporter":[218],"subjected":[221],"unilateral":[223],"ischemia-reperfusion":[224],"injury":[225],"(IRI)":[226],"exhibited":[227],"increased":[228],"colocalized":[231],"compared":[234],"contralateral":[236],"year":[239],"post-injury.":[240],"Notably,":[241],"pharmacologic":[242],"inhibition":[243],"endogenous":[245,266],"synthesis":[247],"conferred":[248],"renal":[249],"protection":[250],"suppressed":[252],"bilateral":[255],"IRI":[256],"cisplatin-induced":[258],"AKI":[259],"mouse":[260],"models.":[261],"Conclusion:":[262],"These":[263],"findings":[264],"driver":[271],"cellular":[273],"aging":[276],"injured":[278],"Inhibiting":[280],"production":[282],"may":[283],"provide":[284],"therapeutic":[287],"strategy":[288],"mitigate":[290],"CKD":[291],"progression":[292],"associated":[293],"normal":[295],"or":[296],"accelerated":[297],"Funding:":[299],"Other":[300],"NIH":[301],"Support":[302,309],"-":[303],"SA":[304],"OAIC-RL5":[305],"award,":[306],"Veterans":[307],"Affairs":[308]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2025-12-01T00:00:00"}