{"id":"https://openalex.org/W4416632490","doi":"https://doi.org/10.5256/f1000research.181081.r410896","title":"Peer Review Report For: Unveiling the Fertility Potential of Brassica oleracea: In Silico and In vivo Insights into Protein Kinase B (PKB/AKT1) and Epidermal Growth Factor Receptor (EGFR) Inhibition [version 1; peer review: 1 approved with reservations]","display_name":"Peer Review Report For: Unveiling the Fertility Potential of Brassica oleracea: In Silico and In vivo Insights into Protein Kinase B (PKB/AKT1) and Epidermal Growth Factor Receptor (EGFR) Inhibition [version 1; peer review: 1 approved with reservations]","publication_year":2025,"publication_date":"2025-10-03","ids":{"openalex":"https://openalex.org/W4416632490","doi":"https://doi.org/10.5256/f1000research.181081.r410896"},"language":"en","primary_location":{"id":"doi:10.5256/f1000research.181081.r410896","doi":"https://doi.org/10.5256/f1000research.181081.r410896","is_oa":true,"landing_page_url":"https://doi.org/10.5256/f1000research.181081.r410896","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"University of Maryland, College Park. USA","raw_type":"peer-review"},"volume":null,"type":"peer-review","indexed_in":["crossref","datacite"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.5256/f1000research.181081.r410896","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5098955412","display_name":"Emmanuel Orire Ikuomola","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2752562790","display_name":"Kampala International University","ror":"https://ror.org/017g82c94","country_code":"UG","type":"education","lineage":["https://openalex.org/I2752562790"]}],"countries":["UG"],"is_corresponding":true,"raw_author_name":"Emmanuel  Orire Ikuomola","raw_affiliation_strings":["Department of Physiology, Kampala International University - Western Campus, Bushenyi, Western Region, 20000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, Kampala International University - Western Campus, Bushenyi, Western Region, 20000","institution_ids":["https://openalex.org/I2752562790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012119442","display_name":"Daniel Udofia Owu","orcid":"https://orcid.org/0000-0002-8264-9131","observed_orcids":["https://orcid.org/0000-0002-8264-9131"]},"institutions":[{"id":"https://openalex.org/I2752562790","display_name":"Kampala International University","ror":"https://ror.org/017g82c94","country_code":"UG","type":"education","lineage":["https://openalex.org/I2752562790"]}],"countries":["UG"],"is_corresponding":false,"raw_author_name":"Daniel Udofia Owu","raw_affiliation_strings":["Department of Physiology, Kampala International University - Western Campus, Bushenyi, Western Region, 20000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, Kampala International University - Western Campus, Bushenyi, Western Region, 20000","institution_ids":["https://openalex.org/I2752562790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049191688","display_name":"Victor Otu Oka","orcid":"https://orcid.org/0000-0001-7230-6293","observed_orcids":["https://orcid.org/0000-0001-7230-6293"]},"institutions":[{"id":"https://openalex.org/I2752562790","display_name":"Kampala International University","ror":"https://ror.org/017g82c94","country_code":"UG","type":"education","lineage":["https://openalex.org/I2752562790"]}],"countries":["UG"],"is_corresponding":false,"raw_author_name":"Victor Otu Oka","raw_affiliation_strings":["Department of Physiology, Kampala International University - Western Campus, Bushenyi, Western Region, 20000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, Kampala International University - Western Campus, Bushenyi, Western Region, 20000","institution_ids":["https://openalex.org/I2752562790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115665406","display_name":"Umar Uthman Shehu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2752562790","display_name":"Kampala International University","ror":"https://ror.org/017g82c94","country_code":"UG","type":"education","lineage":["https://openalex.org/I2752562790"]}],"countries":["UG"],"is_corresponding":false,"raw_author_name":"Umar Uthman Shehu","raw_affiliation_strings":["Department of Physiology, Kampala International University - Western Campus, Bushenyi, Western Region, 20000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, Kampala International University - Western Campus, Bushenyi, Western Region, 20000","institution_ids":["https://openalex.org/I2752562790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115942556","display_name":"Ismahil Adekunle Adeniyi","orcid":"https://orcid.org/0000-0003-3279-2711","observed_orcids":["https://orcid.org/0000-0003-3279-2711"]},"institutions":[{"id":"https://openalex.org/I2752562790","display_name":"Kampala International University","ror":"https://ror.org/017g82c94","country_code":"UG","type":"education","lineage":["https://openalex.org/I2752562790"]}],"countries":["UG"],"is_corresponding":false,"raw_author_name":"Ismahil Adekunle Adeniyi","raw_affiliation_strings":["Department of Physiology, Kampala International University - Western Campus, Bushenyi, Western Region, 20000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, Kampala International University - Western Campus, Bushenyi, Western Region, 20000","institution_ids":["https://openalex.org/I2752562790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054419695","display_name":"Ilemobayo Victor Fasogbon","orcid":"https://orcid.org/0000-0003-4362-9004","observed_orcids":["https://orcid.org/0000-0003-4362-9004"]},"institutions":[{"id":"https://openalex.org/I2752562790","display_name":"Kampala International University","ror":"https://ror.org/017g82c94","country_code":"UG","type":"education","lineage":["https://openalex.org/I2752562790"]}],"countries":["UG"],"is_corresponding":false,"raw_author_name":"Ilemobayo Victor Fasogbon","raw_affiliation_strings":["Department of Biochemistry, Kampala International University - Western Campus, Bushenyi, Western Region, 20000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Kampala International University - Western Campus, Bushenyi, Western Region, 20000","institution_ids":["https://openalex.org/I2752562790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5121695066","display_name":"Ibe Micheal Usman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2752562790","display_name":"Kampala International University","ror":"https://ror.org/017g82c94","country_code":"UG","type":"education","lineage":["https://openalex.org/I2752562790"]}],"countries":["UG"],"is_corresponding":false,"raw_author_name":"Ibe Micheal Usman","raw_affiliation_strings":["Department of Anatomy, Kampala International University - Western Campus, Bushenyi, Western Region, 20000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy, Kampala International University - Western Campus, Bushenyi, Western Region, 20000","institution_ids":["https://openalex.org/I2752562790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033686768","display_name":"Ekom Monday Etukudo","orcid":"https://orcid.org/0009-0002-3527-715X","observed_orcids":["https://orcid.org/0009-0002-3527-715X"]},"institutions":[{"id":"https://openalex.org/I2752562790","display_name":"Kampala International University","ror":"https://ror.org/017g82c94","country_code":"UG","type":"education","lineage":["https://openalex.org/I2752562790"]}],"countries":["UG"],"is_corresponding":false,"raw_author_name":"Ekom Monday Etukudo","raw_affiliation_strings":["Department of Anatomy, Kampala International University - Western Campus, Bushenyi, Western Region, 20000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy, Kampala International University - Western Campus, Bushenyi, Western Region, 20000","institution_ids":["https://openalex.org/I2752562790"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5093899539","display_name":"Patrick Maduabuchi Aja","orcid":"https://orcid.org/0000-0001-7805-9027","observed_orcids":["https://orcid.org/0000-0001-7805-9027"]},"institutions":[{"id":"https://openalex.org/I2752562790","display_name":"Kampala International University","ror":"https://ror.org/017g82c94","country_code":"UG","type":"education","lineage":["https://openalex.org/I2752562790"]}],"countries":["UG"],"is_corresponding":false,"raw_author_name":"Patrick Maduabuchi Aja","raw_affiliation_strings":["Department of Biochemistry, Kampala International University - Western Campus, Bushenyi, Western Region, 20000"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Kampala International University - Western Campus, Bushenyi, Western Region, 20000","institution_ids":["https://openalex.org/I2752562790"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5098955412"],"corresponding_institution_ids":["https://openalex.org/I2752562790"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10211","display_name":"Computational Drug Discovery Methods","score":0.004000000189989805,"subfield":{"id":"https://openalex.org/subfields/1703","display_name":"Computational Theory and Mathematics"},"field":{"id":"https://openalex.org/fields/17","display_name":"Computer Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T14521","display_name":"Medicinal Plant Research","score":0.00279999990016222,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/brassica-oleracea-var-viridis","display_name":"Brassica oleracea var. viridis","score":0.9950000047683716},{"id":"https://openalex.org/keywords/collard-greens","display_name":"collard greens","score":0.9539999961853027},{"id":"https://openalex.org/keywords/male-infertility","display_name":"male infertility","score":0.8500000238418579},{"id":"https://openalex.org/keywords/egfr-inhibition","display_name":"EGFR inhibition","score":0.9959999918937683},{"id":"https://openalex.org/keywords/spermatogenesis","display_name":"spermatogenesis","score":0.8450000286102295},{"id":"https://openalex.org/keywords/gamma-sitosterol","display_name":"\u03b3-sitosterol","score":0.8230000138282776},{"id":"https://openalex.org/keywords/sperm-motility","display_name":"sperm motility","score":0.9120000004768372},{"id":"https://openalex.org/keywords/sperm-viability","display_name":"sperm viability","score":0.42899999022483826},{"id":"https://openalex.org/keywords/phytochemicals","display_name":"phytochemicals","score":0.7630000114440918},{"id":"https://openalex.org/keywords/ethanolic-leaf-extract","display_name":"ethanolic leaf extract","score":0.5580000281333923}],"concepts":[{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.8568000197410583},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.6707000136375427},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6445000171661377},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4943000078201294},{"id":"https://openalex.org/C2776747608","wikidata":"https://www.wikidata.org/wiki/Q58677","display_name":"Brassica","level":2,"score":0.4528999924659729},{"id":"https://openalex.org/C518429986","wikidata":"https://www.wikidata.org/wiki/Q964401","display_name":"Fertility","level":3,"score":0.4339999854564667},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.41830000281333923},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.4122999906539917},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3472999930381775},{"id":"https://openalex.org/C6902291","wikidata":"https://www.wikidata.org/wiki/Q417889","display_name":"Maturation promoting factor","level":5,"score":0.32839998602867126},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.32100000977516174},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.3190000057220459},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.30230000615119934},{"id":"https://openalex.org/C2779438470","wikidata":"https://www.wikidata.org/wiki/Q424401","display_name":"Epidermal growth factor receptor","level":3,"score":0.28940001130104065},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.27799999713897705},{"id":"https://openalex.org/C26375932","wikidata":"https://www.wikidata.org/wiki/Q118226","display_name":"Growth factor receptor","level":3,"score":0.273499995470047},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.26739999651908875},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26100000739097595}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.5256/f1000research.181081.r410896","doi":"https://doi.org/10.5256/f1000research.181081.r410896","is_oa":true,"landing_page_url":"https://doi.org/10.5256/f1000research.181081.r410896","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"University of Maryland, College Park. USA","raw_type":"peer-review"},{"id":"doi:10.5256/f1000research.181081.r410896","doi":"https://doi.org/10.5256/f1000research.181081.r410896","is_oa":true,"landing_page_url":"https://doi.org/10.5256/f1000research.181081.r410896","pdf_url":null,"source":{"id":"https://openalex.org/S7407050752","display_name":"Faculty of 1000 Research Ltd","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":null,"raw_source_name":null,"raw_type":"PeerReview"}],"best_oa_location":{"id":"doi:10.5256/f1000research.181081.r410896","doi":"https://doi.org/10.5256/f1000research.181081.r410896","is_oa":true,"landing_page_url":"https://doi.org/10.5256/f1000research.181081.r410896","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"University of Maryland, College Park. USA","raw_type":"peer-review"},"sustainable_development_goals":[{"score":0.8999999761581421,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"},{"score":0.5365999937057495,"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Background":[0],"Infertility":[1],"is":[2],"a":[3,82],"global":[4],"health":[5,248],"issue,":[6],"with":[7,49],"male":[8,246],"factor":[9],"infertility":[10],"contributing":[11],"to":[12,111,148,157],"nearly":[13],"50%":[14],"of":[15,18,125,190,197,205],"cases.":[16],"Dysregulation":[17],"Protein":[19],"Kinase":[20],"B":[21],"(PKB/AKT1)":[22],"and":[23,51,71,77,98,103,113,128,137,152,166,182,200,218,233],"Epidermal":[24],"Growth":[25],"Factor":[26],"Receptor":[27],"(EGFR)":[28],"signaling":[29],"impairs":[30],"spermatogenesis.":[31],"Bioactive":[32,90],"compounds":[33,63,91,226],"offer":[34],"promising":[35],"alternatives":[36],"for":[37,100,169,245],"targeting":[38],"these":[39],"pathways.":[40],"Brassica":[41,191],"oleracea":[42,66,175,222,243],"var.":[43,67,223],"viridis":[44,68,224],"(collard":[45],"greens)":[46],"contains":[47],"phytochemicals":[48],"antioxidant":[50],"anti-inflammatory":[52],"properties,":[53],"suggesting":[54],"potential":[55],"reproductive":[56,86,247],"benefits.":[57],"Objective":[58],"This":[59],"study":[60],"evaluates":[61],"bioactive":[62,225],"from":[64],"B.":[65,174,221,242],"as":[69],"AKT1":[70,112,149,232],"EGFR":[72,153,234],"inhibitors":[73],"through":[74],"molecular":[75],"docking":[76,107],"in":[78,81,117,120],"vivo":[79,118],"validation":[80,119],"cimetidine":[83],"(Cemet":[84],"\u00ae)-induced":[85],"toxicity":[87,168],"model.":[88],"Methods":[89],"were":[92,139],"identified":[93],"via":[94,231],"Gas":[95],"Chromatography-Mass":[96],"Spectrometry":[97],"analyzed":[99],"physicochemical,":[101],"pharmacokinetic,":[102],"pharmacodynamic":[104],"properties.":[105],"Molecular":[106],"assessed":[108],"binding":[109,146],"affinity":[110,147],"EGFR,":[114],"followed":[115],"by":[116],"cimetidine-exposed":[121],"Wistar":[122],"rats.":[123],"Effects":[124],"ethanol":[126],"extracts":[127,176],"solvent":[129],"fractions":[130,196,204,208],"on":[131],"sperm":[132,179,216],"motility,":[133,180],"viability,":[134,181],"morphology,":[135],"count,":[136,183],"agglutination":[138],"examined.":[140],"Results":[141],"Gamma-sitosterol":[142],"showed":[143],"the":[144,210],"strongest":[145],"(-8.0":[150],"kcal/mol)":[151],"(-6.5":[154],"kcal/mol),":[155],"comparable":[156],"co-crystallized":[158],"ligands.":[159],"Computational":[160],"analysis":[161],"indicated":[162],"high":[163],"Gastro-intestinal":[164],"absorption":[165],"low":[167],"most":[170,211],"compounds.":[171],"In":[172],"vivo,":[173],"significantly":[177],"improved":[178],"mitigating":[184],"cimetidine-induced":[185],"toxicity.":[186],"Ethanol":[187],"Leaf":[188],"Extract":[189],"Oleracea":[192],"(ELEBO),":[193],"AFBO":[194],"(Aqueous":[195],"brassica":[198,206],"Oleracea)":[199,207],"BFBO":[201],"(n-":[202],"Butanol":[203],"had":[209],"pronounced":[212],"protective":[213],"effects,":[214,229],"reducing":[215],"abnormalities":[217],"agglutination.":[219],"Conclusion":[220],"show":[227],"spermatoprotective":[228],"likely":[230],"inhibition.":[235],"These":[236],"findings":[237],"support":[238],"further":[239],"research":[240],"into":[241],"derivatives":[244],"applications.":[249]},"counts_by_year":[],"updated_date":"2026-10-13T10:08:12.033832","created_date":"2025-10-10T00:00:00"}