{"id":"https://openalex.org/W4416265373","doi":"https://doi.org/10.1016/j.jbc.2025.110946","title":"Scalable purification enables high-quality virus-like particles for therapeutic translation","display_name":"Scalable purification enables high-quality virus-like particles for therapeutic translation","publication_year":2025,"publication_date":"2025-11-17","ids":{"openalex":"https://openalex.org/W4416265373","doi":"https://doi.org/10.1016/j.jbc.2025.110946","pmid":"https://pubmed.ncbi.nlm.nih.gov/41241097","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/12753231"},"language":"en","primary_location":{"id":"doi:10.1016/j.jbc.2025.110946","doi":"https://doi.org/10.1016/j.jbc.2025.110946","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbc.2025.110946","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.jbc.2025.110946","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029538270","display_name":"Rafa\u0142 Ho\u0142ubowicz","orcid":"https://orcid.org/0000-0002-2555-2069","observed_orcids":["https://orcid.org/0000-0002-2555-2069"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Rafal Ho\u0142ubowicz","raw_affiliation_strings":["Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA. Electronic address: rholubow@uci.edu"],"raw_orcid":"https://orcid.org/0000-0002-2555-2069","affiliations":[{"raw_affiliation_string":"Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA. Electronic address: rholubow@uci.edu","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076071784","display_name":"Fangyuan Gao","orcid":"https://orcid.org/0000-0002-1270-8781","observed_orcids":["https://orcid.org/0000-0002-1270-8781"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Fangyuan Gao","raw_affiliation_strings":["Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017998058","display_name":"Samuel W. Du","orcid":"https://orcid.org/0000-0002-9655-3659","observed_orcids":["https://orcid.org/0000-0002-9655-3659"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Samuel W. Du","raw_affiliation_strings":["Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA; Department of Physiology and Biophysics, University of California Irvine, Irvine, California, USA"],"raw_orcid":"https://orcid.org/0000-0002-9655-3659","affiliations":[{"raw_affiliation_string":"Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA; Department of Physiology and Biophysics, University of California Irvine, Irvine, California, USA","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075544482","display_name":"Carolline Rodrigues Menezes","orcid":"https://orcid.org/0000-0001-6381-0543","observed_orcids":["https://orcid.org/0000-0001-6381-0543"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Carolline Rodrigues Menezes","raw_affiliation_strings":["Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA; Department of Physiology and Biophysics, University of California Irvine, Irvine, California, USA"],"raw_orcid":"https://orcid.org/0000-0001-6381-0543","affiliations":[{"raw_affiliation_string":"Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA; Department of Physiology and Biophysics, University of California Irvine, Irvine, California, USA","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100693219","display_name":"Jianye Zhang","orcid":"https://orcid.org/0000-0002-9579-1399","observed_orcids":["https://orcid.org/0000-0002-9579-1399"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jianye Zhang","raw_affiliation_strings":["Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114872626","display_name":"Maria W. Ho\u0142ubowicz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maria W. Ho\u0142ubowicz","raw_affiliation_strings":["Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5105665614","display_name":"Paul Chen","orcid":"https://orcid.org/0000-0001-5261-1610","observed_orcids":["https://orcid.org/0000-0001-5261-1610"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]},{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4390039253","display_name":"Koch Institute for Integrative Cancer Research At MIT","ror":"https://ror.org/01xd6q208","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039253","https://openalex.org/I63966007"]},{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paul Z. Chen","raw_affiliation_strings":["Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I1344073410","https://openalex.org/I136199984","https://openalex.org/I4390039253","https://openalex.org/I63966007"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068420939","display_name":"Niklas Armbrust","orcid":"https://orcid.org/0000-0002-7396-4949","observed_orcids":["https://orcid.org/0000-0002-7396-4949"]},"institutions":[{"id":"https://openalex.org/I3018134672","display_name":"Helmholtz Munich","ror":"https://ror.org/00cfam450","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I3018134672"]},{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Niklas Armbrust","raw_affiliation_strings":["Institute for Synthetic Biomedicine, Helmholtz Munich, Neuherberg, Germany; Department of Bioscience, TUM School of Natural Sciences, Technical University of Munich, Munich, Germany"],"raw_orcid":"https://orcid.org/0000-0002-7396-4949","affiliations":[{"raw_affiliation_string":"Institute for Synthetic Biomedicine, Helmholtz Munich, Neuherberg, Germany; Department of Bioscience, TUM School of Natural Sciences, Technical University of Munich, Munich, Germany","institution_ids":["https://openalex.org/I3018134672","https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045097933","display_name":"Julian Geilenkeuser","orcid":"https://orcid.org/0000-0001-5714-7466","observed_orcids":["https://orcid.org/0000-0001-5714-7466"]},"institutions":[{"id":"https://openalex.org/I3018134672","display_name":"Helmholtz Munich","ror":"https://ror.org/00cfam450","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I3018134672"]},{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Julian Geilenkeuser","raw_affiliation_strings":["Institute for Synthetic Biomedicine, Helmholtz Munich, Neuherberg, Germany; Department of Bioscience, TUM School of Natural Sciences, Technical University of Munich, Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Synthetic Biomedicine, Helmholtz Munich, Neuherberg, Germany; Department of Bioscience, TUM School of Natural Sciences, Technical University of Munich, Munich, Germany","institution_ids":["https://openalex.org/I3018134672","https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065535610","display_name":"David R. Liu","orcid":"https://orcid.org/0000-0002-9943-7557","observed_orcids":["https://orcid.org/0000-0002-9943-7557","https://orcid.org/0000-0003-0346-5033","https://orcid.org/0000-0002-6078-6506"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]},{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David R. Liu","raw_affiliation_strings":["Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA","institution_ids":["https://openalex.org/I107606265","https://openalex.org/I1344073410","https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058816989","display_name":"Dong\u2010Jiunn Jeffery Truong","orcid":"https://orcid.org/0000-0003-1722-8573","observed_orcids":["https://orcid.org/0000-0003-1722-8573"]},"institutions":[{"id":"https://openalex.org/I3018134672","display_name":"Helmholtz Munich","ror":"https://ror.org/00cfam450","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I3018134672"]},{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Dong-Jiunn Jeffery Truong","raw_affiliation_strings":["Institute for Synthetic Biomedicine, Helmholtz Munich, Neuherberg, Germany; Department of Bioscience, TUM School of Natural Sciences, Technical University of Munich, Munich, Germany"],"raw_orcid":"https://orcid.org/0000-0003-1722-8573","affiliations":[{"raw_affiliation_string":"Institute for Synthetic Biomedicine, Helmholtz Munich, Neuherberg, Germany; Department of Bioscience, TUM School of Natural Sciences, Technical University of Munich, Munich, Germany","institution_ids":["https://openalex.org/I3018134672","https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039343293","display_name":"Gil G. Westmeyer","orcid":"https://orcid.org/0000-0001-7224-8919","observed_orcids":["https://orcid.org/0000-0001-7224-8919"]},"institutions":[{"id":"https://openalex.org/I3018134672","display_name":"Helmholtz Munich","ror":"https://ror.org/00cfam450","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I3018134672"]},{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Gil Gregor Westmeyer","raw_affiliation_strings":["Institute for Synthetic Biomedicine, Helmholtz Munich, Neuherberg, Germany; Department of Bioscience, TUM School of Natural Sciences, Technical University of Munich, Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Synthetic Biomedicine, Helmholtz Munich, Neuherberg, Germany; Department of Bioscience, TUM School of Natural Sciences, Technical University of Munich, Munich, Germany","institution_ids":["https://openalex.org/I3018134672","https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035243943","display_name":"Gra\u017cyna Palczewska","orcid":"https://orcid.org/0000-0003-4030-4685","observed_orcids":["https://orcid.org/0000-0003-4030-4685"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Grazyna Palczewska","raw_affiliation_strings":["Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA","institution_ids":["https://openalex.org/I204250578"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045657649","display_name":"Krzysztof Palczewski","orcid":"https://orcid.org/0000-0002-0788-545X","observed_orcids":["https://orcid.org/0000-0002-0788-545X"]},"institutions":[{"id":"https://openalex.org/I204250578","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771","country_code":"US","type":"education","lineage":["https://openalex.org/I204250578"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Krzysztof Palczewski","raw_affiliation_strings":["Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA; Department of Physiology and Biophysics, University of California Irvine, Irvine, California, USA; Department of Chemistry, University of California Irvine, Irvine, California, USA; Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, USA. Electronic address: kpalczew@uci.edu"],"raw_orcid":"https://orcid.org/0000-0002-0788-545X","affiliations":[{"raw_affiliation_string":"Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, California, USA; Department of Physiology and Biophysics, University of California Irvine, Irvine, California, USA; Department of Chemistry, University of California Irvine, Irvine, California, USA; Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, USA. Electronic address: kpalczew@uci.edu","institution_ids":["https://openalex.org/I204250578"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":8,"corresponding_author_ids":["https://openalex.org/A5029538270","https://openalex.org/A5045657649"],"corresponding_institution_ids":["https://openalex.org/I204250578"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":3.4723,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.93711567,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":98,"max":99},"biblio":{"volume":"301","issue":"12","first_page":"110946","last_page":"110946"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.4657999873161316,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.4657999873161316,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.2313999980688095,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10063","display_name":"Nanoparticle-Based Drug Delivery","score":0.1080000028014183,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/virus-like-particles","display_name":"virus-like particles","score":0.9900000095367432},{"id":"https://openalex.org/keywords/vlp-purification","display_name":"VLP purification","score":0.847000002861023},{"id":"https://openalex.org/keywords/chromatographic-purification","display_name":"chromatographic purification","score":0.8059999942779541},{"id":"https://openalex.org/keywords/host-cell-protein-removal","display_name":"host cell protein removal","score":0.7680000066757202},{"id":"https://openalex.org/keywords/scalable-fabrication","display_name":"scalable fabrication","score":0.4970000088214874},{"id":"https://openalex.org/keywords/intracellular-protein-delivery","display_name":"intracellular protein delivery","score":0.8149999976158142},{"id":"https://openalex.org/keywords/clinical-translation","display_name":"clinical translation","score":0.6029999852180481}],"concepts":[{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.4975000023841858},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.4893999993801117},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4862000048160553},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4684999883174896},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.4372999966144562},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4124000072479248},{"id":"https://openalex.org/C8985478","wikidata":"https://www.wikidata.org/wiki/Q903485","display_name":"Protein purification","level":2,"score":0.39980000257492065},{"id":"https://openalex.org/C54689828","wikidata":"https://www.wikidata.org/wiki/Q2029259","display_name":"Ultracentrifuge","level":2,"score":0.3937000036239624},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.3684999942779541},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.3262999951839447},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.3215999901294708},{"id":"https://openalex.org/C49853544","wikidata":"https://www.wikidata.org/wiki/Q206229","display_name":"Biomolecule","level":2,"score":0.3181999921798706},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.3050000071525574},{"id":"https://openalex.org/C155672457","wikidata":"https://www.wikidata.org/wiki/Q61231","display_name":"Nanoparticle","level":2,"score":0.30329999327659607},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.290800005197525},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.288100004196167},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28139999508857727},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.2799000144004822},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.27090001106262207},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.26269999146461487},{"id":"https://openalex.org/C2780430158","wikidata":"https://www.wikidata.org/wiki/Q7685902","display_name":"Target protein","level":3,"score":0.2590000033378601},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.25780001282691956},{"id":"https://openalex.org/C15083742","wikidata":"https://www.wikidata.org/wiki/Q261659","display_name":"Nanomedicine","level":3,"score":0.2572000026702881},{"id":"https://openalex.org/C48044578","wikidata":"https://www.wikidata.org/wiki/Q727490","display_name":"Scalability","level":2,"score":0.2549000084400177}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009052","descriptor_name":"Leukemia Virus, Murine","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014461","descriptor_name":"Ultracentrifugation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014771","descriptor_name":"Virion","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058425","descriptor_name":"Vaccines, Virus-Like Particle","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1016/j.jbc.2025.110946","doi":"https://doi.org/10.1016/j.jbc.2025.110946","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbc.2025.110946","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:41241097","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41241097","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12753231","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12753231/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biol Chem","raw_type":"Text"},{"id":"pmh:oai:escholarship.org:ark:/13030/qt9mw572rg","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/9mw572rg","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Biological Chemistry, vol 301, iss 12","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.jbc.2025.110946","doi":"https://doi.org/10.1016/j.jbc.2025.110946","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbc.2025.110946","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8199999928474426}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2533832979","display_name":"Delivery Technologies for In Vivo Genome Editing","funder_award_id":"UG3AI150551","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G269652695","display_name":null,"funder_award_id":"TA-GT-0423\\u20130847-UCI-TRAP","funder_id":"https://openalex.org/F4320306340","funder_display_name":"Foundation Fighting Blindness"},{"id":"https://openalex.org/G3570214930","display_name":null,"funder_award_id":"R01EY036994","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G4237274230","display_name":"Expanding the Scope of Base Editing","funder_award_id":"U01AI142756","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G694063290","display_name":"Medical Scientist Training Program","funder_award_id":"T32GM008620","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G7032010522","display_name":null,"funder_award_id":"TA-GT-0624-0887-UCI","funder_id":"https://openalex.org/F4320306340","funder_display_name":"Foundation Fighting Blindness"},{"id":"https://openalex.org/G853170728","display_name":null,"funder_award_id":"P30EY034070","funder_id":"https://openalex.org/F4320306811","funder_display_name":"Research to Prevent Blindness"},{"id":"https://openalex.org/G9000858696","display_name":"Editing AMD Risk Alleles in Human Cells","funder_award_id":"1r01ey036994-01","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000892366","display_name":"Retinoids in Vision","funder_award_id":"R01EY009339","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G9000899223","display_name":"microRNA-204 and microRNA-211 regulation of RPE phagocytosis","funder_award_id":"F30EY033642","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G9000908732","display_name":"Integrating Chemistry and Evolution to Illuminate Biology and Enable Novel Therapeutics","funder_award_id":"R35GM118062","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G9000912514","display_name":"Center for Genomic Editing and Recording: Development and Application of Next-Generation Genome and Epigenome Editing Methods to Advance the Study and Treatment of Human Disease","funder_award_id":"RM1HG009490","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G9000913315","display_name":"Precision genome editing in vivo to treat retinal diseases","funder_award_id":"R01EY034501","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"},{"id":"https://openalex.org/G9000915136","display_name":"NEI UCI Center Core Grant for Vision Research","funder_award_id":"P30EY034070","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"}],"funders":[{"id":"https://openalex.org/F4320306082","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90"},{"id":"https://openalex.org/F4320306253","display_name":"Arnold and Mabel Beckman Foundation","ror":"https://ror.org/05avmtm72"},{"id":"https://openalex.org/F4320306340","display_name":"Foundation Fighting Blindness","ror":"https://ror.org/011728q79"},{"id":"https://openalex.org/F4320306404","display_name":"Knights Templar Eye Foundation","ror":"https://ror.org/03mq5pg65"},{"id":"https://openalex.org/F4320306811","display_name":"Research to Prevent Blindness","ror":"https://ror.org/04drjs621"},{"id":"https://openalex.org/F4320316572","display_name":"Schmidt Family Foundation","ror":"https://ror.org/014bj2y47"},{"id":"https://openalex.org/F4320316902","display_name":"UC Irvine Materials Research Institute","ror":null},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332688","display_name":"University of California, Irvine","ror":"https://ror.org/04gyf1771"},{"id":"https://openalex.org/F4320334593","display_name":"Natural Sciences and Engineering Research Council of Canada","ror":"https://ror.org/01h531d29"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"},{"id":"https://openalex.org/F4320337350","display_name":"National Eye Institute","ror":"https://ror.org/03wkg3b53"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"},{"id":"https://openalex.org/F4320337890","display_name":"School of Medicine, University of California, Irvine","ror":null}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":69,"referenced_works":["https://openalex.org/W1971792228","https://openalex.org/W2036176224","https://openalex.org/W2041725513","https://openalex.org/W2045435533","https://openalex.org/W2048038866","https://openalex.org/W2092706417","https://openalex.org/W2096655776","https://openalex.org/W2104010513","https://openalex.org/W2162349087","https://openalex.org/W2336828812","https://openalex.org/W2613479531","https://openalex.org/W2766599608","https://openalex.org/W2874925241","https://openalex.org/W2949383259","https://openalex.org/W2977139863","https://openalex.org/W2981137429","https://openalex.org/W3007934773","https://openalex.org/W3038068331","https://openalex.org/W3081420496","https://openalex.org/W3092667519","https://openalex.org/W3111914259","https://openalex.org/W3132217836","https://openalex.org/W3172542442","https://openalex.org/W3176695533","https://openalex.org/W4206390074","https://openalex.org/W4214657247","https://openalex.org/W4224000672","https://openalex.org/W4295760639","https://openalex.org/W4298089037","https://openalex.org/W4366823157","https://openalex.org/W4366998803","https://openalex.org/W4385442675","https://openalex.org/W4389333938","https://openalex.org/W4390670214","https://openalex.org/W4390750892","https://openalex.org/W4391648976","https://openalex.org/W4392546250","https://openalex.org/W4401794452","https://openalex.org/W4403325123","https://openalex.org/W4404046121","https://openalex.org/W4404144042","https://openalex.org/W4404469966","https://openalex.org/W4404824804","https://openalex.org/W4406061191","https://openalex.org/W4406097465","https://openalex.org/W4406512367","https://openalex.org/W4406681254","https://openalex.org/W4406822201","https://openalex.org/W4406870678","https://openalex.org/W4406872946","https://openalex.org/W4407295816","https://openalex.org/W4408059497","https://openalex.org/W4408121735","https://openalex.org/W4408579919","https://openalex.org/W4409283383","https://openalex.org/W4409570679","https://openalex.org/W4410051645","https://openalex.org/W4410396192","https://openalex.org/W4410521520","https://openalex.org/W4410967089","https://openalex.org/W4411451896","https://openalex.org/W4411966605","https://openalex.org/W4412397486","https://openalex.org/W4412486643","https://openalex.org/W4412491500","https://openalex.org/W4412556518","https://openalex.org/W4412650647","https://openalex.org/W4413051598","https://openalex.org/W4413817845"],"related_works":["https://openalex.org/W7152985603","https://openalex.org/W4408260639","https://openalex.org/W4307702578","https://openalex.org/W4406512367","https://openalex.org/W7148414079","https://openalex.org/W7223175139","https://openalex.org/W1981752210","https://openalex.org/W1813361023","https://openalex.org/W2289453066","https://openalex.org/W2792390893"],"abstract_inverted_index":{"Emerging":[0],"molecular":[1],"therapies":[2],"introduce":[3],"enzymatic":[4],"activity":[5],"into":[6],"cells":[7],"by":[8,50,152],"delivering":[9],"genes,":[10],"transcripts,":[11],"or":[12],"proteins.":[13],"Owing":[14],"to":[15,148],"their":[16],"robust":[17,207],"cell-entry":[18],"capacity,":[19],"virus-like":[20],"particles":[21],"(VLPs)":[22],"represent":[23],"a":[24,74,111,160,197],"technology":[25],"of":[26,34,45,73,86,93,106,170,203,209],"choice":[27],"in":[28,163],"genome":[29,217],"editing,":[30,218],"where":[31],"low":[32],"doses":[33],"heterologous":[35],"proteins":[36,124,167],"and":[37,65,83,99,116,125,132,143,195,221],"nucleic":[38],"acids":[39],"are":[40],"essential.":[41],"However,":[42],"clinical":[43],"translation":[44],"VLP":[46,130,204],"vectors":[47],"is":[48],"hindered":[49],"inadequate":[51],"purification":[52,77,189],"methods.":[53,155],"Current":[54],"approaches,":[55],"relying":[56],"primarily":[57],"on":[58],"ultracentrifugation,":[59],"suffer":[60],"from":[61],"inconsistent":[62],"product":[63],"quality":[64],"poor":[66],"scalability.":[67],"Here,":[68],"we":[69,109],"report":[70],"the":[71,81,90,171],"development":[72,202],"broadly":[75],"applicable":[76],"strategy":[78],"that":[79,113,224],"improves":[80],"purity":[82],"therapeutic":[84,179],"efficacy":[85],"genome-editing":[87],"VLPs.":[88],"Considering":[89],"characteristic":[91],"properties":[92],"murine":[94],"leukemia":[95],"virus-derived":[96],"engineered":[97,101],"VLPs":[98,137,149,184,211],"HIV-derived":[100],"nucleocytosolic":[102],"vehicles":[103],"for":[104,200,212],"loading":[105],"programmable":[107],"editors,":[108],"developed":[110],"workflow":[112],"involves":[114],"single-modal":[115],"multimodal":[117],"chromatographic":[118],"steps,":[119],"effectively":[120],"removing":[121],"host":[122],"cell":[123],"cell-culture":[126],"contaminants":[127],"while":[128],"improving":[129],"integrity":[131],"biological":[133],"activity.":[134],"Our":[135,187],"purified":[136,151,183],"displayed":[138],"superior":[139],"protein":[140,227],"composition,":[141],"consistency,":[142],"enhanced":[144],"functional":[145],"delivery":[146],"compared":[147],"partially":[150],"conventional":[153],"ultracentrifugation":[154],"Mass":[156],"spectrometric":[157],"analysis":[158],"revealed":[159],"substantial":[161],"decrease":[162],"contaminants,":[164],"with":[165],"VLP-specific":[166],"comprising":[168],">90%":[169],"final":[172],"product.":[173],"In":[174],"vivo":[175],"studies":[176],"confirmed":[177],"improved":[178],"outcomes":[180],"when":[181],"chromatographically":[182],"were":[185],"used.":[186],"scalable":[188],"platform":[190],"addresses":[191],"critical":[192],"manufacturing":[193],"bottlenecks":[194],"constitutes":[196],"starting":[198],"point":[199],"further":[201],"therapeutics,":[205],"enabling":[206],"production":[208],"pure":[210],"diverse":[213],"applications":[214],"such":[215],"as":[216],"vaccine":[219],"development,":[220],"other":[222],"uses":[223],"require":[225],"intracellular":[226],"delivery.":[228]},"counts_by_year":[{"year":2026,"cited_by_count":6}],"updated_date":"2026-11-03T10:50:19.383937","created_date":"2025-11-17T00:00:00"}