{"id":"https://openalex.org/W4415879334","doi":"https://doi.org/10.22541/essoar.176227299.97348672/v1","title":"Metal stable isotope probing reveals pH driven microbial copper uptake rates in a continental hydrothermal system","display_name":"Metal stable isotope probing reveals pH driven microbial copper uptake rates in a continental hydrothermal system","publication_year":2025,"publication_date":"2025-11-04","ids":{"openalex":"https://openalex.org/W4415879334","doi":"https://doi.org/10.22541/essoar.176227299.97348672/v1"},"language":"en","primary_location":{"id":"doi:10.22541/essoar.176227299.97348672/v1","doi":"https://doi.org/10.22541/essoar.176227299.97348672/v1","is_oa":false,"landing_page_url":"https://doi.org/10.22541/essoar.176227299.97348672/v1","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5093464873","display_name":"Eva Andrade\u2010Barahona","orcid":"https://orcid.org/0009-0003-9096-0768","observed_orcids":["https://orcid.org/0009-0003-9096-0768"]},"institutions":[{"id":"https://openalex.org/I917168660","display_name":"Montana Technological University","ror":"https://ror.org/03ttqt747","country_code":"US","type":"education","lineage":["https://openalex.org/I917168660"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eva Andrade-Barahona","raw_affiliation_strings":["Montana Technological University"],"raw_orcid":"https://orcid.org/0009-0003-9096-0768","affiliations":[{"raw_affiliation_string":"Montana Technological University","institution_ids":["https://openalex.org/I917168660"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036623707","display_name":"Paul Helfrich","orcid":"https://orcid.org/0000-0002-1601-9289","observed_orcids":["https://orcid.org/0000-0002-1601-9289"]},"institutions":[{"id":"https://openalex.org/I917168660","display_name":"Montana Technological University","ror":"https://ror.org/03ttqt747","country_code":"US","type":"education","lineage":["https://openalex.org/I917168660"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paul Goldsborough Helfrich","raw_affiliation_strings":["Montana Technological University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Montana Technological University","institution_ids":["https://openalex.org/I917168660"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Jacqueline Timmer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405262450","display_name":"Montana Bureau of Mines and Geology","ror":"https://ror.org/03db6jy14","country_code":"US","type":"government","lineage":["https://openalex.org/I4405262450","https://openalex.org/I917168660"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jacqueline Timmer","raw_affiliation_strings":["Montana Bureaur of Mines and Geology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Montana Bureaur of Mines and Geology","institution_ids":["https://openalex.org/I4405262450"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101773115","display_name":"Alysia D. Cox","orcid":"https://orcid.org/0000-0001-9108-143X","observed_orcids":["https://orcid.org/0000-0001-9108-143X"]},"institutions":[{"id":"https://openalex.org/I917168660","display_name":"Montana Technological University","ror":"https://ror.org/03ttqt747","country_code":"US","type":"education","lineage":["https://openalex.org/I917168660"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alysia Cox","raw_affiliation_strings":["Montana Technological University"],"raw_orcid":"https://orcid.org/0000-0001-9108-143X","affiliations":[{"raw_affiliation_string":"Montana Technological University","institution_ids":["https://openalex.org/I917168660"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.7070000171661377,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.7070000171661377,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11073","display_name":"Metal Extraction and Bioleaching","score":0.10859999805688858,"subfield":{"id":"https://openalex.org/subfields/2506","display_name":"Metals and Alloys"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11740","display_name":"Geochemistry and Elemental Analysis","score":0.08250000327825546,"subfield":{"id":"https://openalex.org/subfields/1906","display_name":"Geochemistry and Petrology"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ph","display_name":"pH","score":0.4480000138282776},{"id":"https://openalex.org/keywords/hydrothermal-springs","display_name":"hydrothermal springs","score":0.7379999756813049},{"id":"https://openalex.org/keywords/copper-speciation","display_name":"copper speciation","score":0.8349999785423279},{"id":"https://openalex.org/keywords/dissolved-oxygen","display_name":"dissolved oxygen","score":0.9449999928474426}],"concepts":[{"id":"https://openalex.org/C544778455","wikidata":"https://www.wikidata.org/wiki/Q753","display_name":"Copper","level":2,"score":0.8392000198364258},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6941999793052673},{"id":"https://openalex.org/C22117777","wikidata":"https://www.wikidata.org/wiki/Q17148629","display_name":"Stable isotope ratio","level":2,"score":0.6710000038146973},{"id":"https://openalex.org/C544153396","wikidata":"https://www.wikidata.org/wiki/Q11426","display_name":"Metal","level":2,"score":0.64410001039505},{"id":"https://openalex.org/C107872376","wikidata":"https://www.wikidata.org/wiki/Q321355","display_name":"Environmental chemistry","level":1,"score":0.4952999949455261},{"id":"https://openalex.org/C164304813","wikidata":"https://www.wikidata.org/wiki/Q25276","display_name":"Isotope","level":2,"score":0.4417000114917755},{"id":"https://openalex.org/C156622251","wikidata":"https://www.wikidata.org/wiki/Q1036221","display_name":"Hydrothermal circulation","level":2,"score":0.4088999927043915},{"id":"https://openalex.org/C14522933","wikidata":"https://www.wikidata.org/wiki/Q531507","display_name":"Micronutrient","level":2,"score":0.33799999952316284},{"id":"https://openalex.org/C179104552","wikidata":"https://www.wikidata.org/wiki/Q11165","display_name":"Inorganic chemistry","level":1,"score":0.3377000093460083},{"id":"https://openalex.org/C2777624702","wikidata":"https://www.wikidata.org/wiki/Q17096563","display_name":"Stable-isotope probing","level":4,"score":0.31619998812675476},{"id":"https://openalex.org/C2776838516","wikidata":"https://www.wikidata.org/wiki/Q187787","display_name":"Genetic algorithm","level":2,"score":0.2971999943256378},{"id":"https://openalex.org/C106773901","wikidata":"https://www.wikidata.org/wiki/Q19588","display_name":"Transition metal","level":3,"score":0.27399998903274536},{"id":"https://openalex.org/C2777822432","wikidata":"https://www.wikidata.org/wiki/Q216681","display_name":"In situ","level":2,"score":0.2702000141143799}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.22541/essoar.176227299.97348672/v1","doi":"https://doi.org/10.22541/essoar.176227299.97348672/v1","is_oa":false,"landing_page_url":"https://doi.org/10.22541/essoar.176227299.97348672/v1","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Clean water and sanitation","score":0.6618000268936157,"id":"https://metadata.un.org/sdg/6"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7124520829","https://openalex.org/W4408506450","https://openalex.org/W4404918561","https://openalex.org/W2973114171","https://openalex.org/W1992222257","https://openalex.org/W1989180099","https://openalex.org/W2039415891","https://openalex.org/W2364055006","https://openalex.org/W2765849583","https://openalex.org/W2100482552"],"abstract_inverted_index":{"Copper":[0],"(Cu)":[1],"is":[2],"an":[3],"essential":[4],"micronutrient":[5],"to":[6,26,50,64,72,86,110],"microorganisms.":[7],"Microbes":[8],"take":[9],"up":[10],"and":[11,76,161,171,194,201],"regulate":[12],"intracellular":[13],"copper":[14,31,77,94,120,125,146,155,167,212],"using":[15],"metal":[16,21,113],"trafficking":[17],"pathways.":[18],"We":[19],"used":[20,109],"stable":[22],"isotope":[23],"probing":[24],"(MSIP)":[25],"track":[27,111],"in":[28,38,145,178,215],"situ":[29],"microbial":[30,93,112,119,166,211],"uptake":[32,95,114,121,126,147,213],"rates":[33,96,148,214],"at":[34,135,149],"five":[35],"hydrothermal":[36,217],"springs":[37],"the":[39,59,81,179,182,204],"Yellowstone":[40],"Plateau":[41],"Volcanic":[42],"Field":[43],"(YPVF).":[44],"Spring":[45,116],"temperatures":[46],"ranged":[47],"between":[48],"29.5":[49],"92.7":[51],"\u00b1":[52,74,88,130],"0.1oC,":[53],"dissolved":[54,163,191],"oxygen":[55,164],"concentrations":[56,78],"from":[57,70,79],"below":[58,80],"3.13x10-7":[60],"mol/L":[61],"detection":[62,84],"limit":[63,85],"8.33":[65],"x10-2":[66],"mol/L,":[67],"pH":[68,117,137,150,208],"values":[69,151],"2.73":[71],"8.81":[73],"0.05,":[75],"0.094":[82],"nM":[83],"4.78":[87],"0.19":[89],"nM.":[90],"Obtaining":[91],"reasonable":[92],"across":[97],"this":[98],"wide":[99],"range":[100],"of":[101,128,174],"geochemical":[102],"conditions":[103],"showed":[104],"that":[105],"MSIP":[106],"can":[107],"be":[108],"rates.":[115,122],"drives":[118],"The":[123],"highest":[124],"rate":[127],"32":[129],"7":[131],"nmol":[132],"d-1L-1":[133],"occurred":[134],"a":[136,142],"5.81":[138],"spring":[139],"source,":[140],"with":[141,198],"sharp":[143],"decrease":[144],"\u2265":[152],"6.14.":[153],"More":[154],"speciated":[156],"as":[157],"Cu2+":[158],"free":[159],"ion":[160],"increased":[162],"enhanced":[165],"uptake.":[168],"Additionally,":[169],"enrichment":[170,200],"depletion":[172,202],"patterns":[173],"46":[175],"elements":[176],"varied":[177],"microbes":[180],"following":[181],"65Cu-enriched":[183],"spike.":[184],"Distance-based":[185],"redundancy":[186],"analysis":[187],"(db-RDA)":[188],"indicated":[189],"pH,":[190],"inorganic":[192],"carbon,":[193],"various":[195],"anions":[196],"correlated":[197],"element":[199],"by":[203],"site\u2019s":[205],"location.":[206],"Overall,":[207],"best":[209],"predicts":[210],"continental":[216],"systems.":[218]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2025-11-04T00:00:00"}