{"id":"https://openalex.org/W4414459798","doi":"https://doi.org/10.1002/ps.70249","title":"Fluazinam binds with the ATP synthase subunit FfATPh but not with FfATP5 or FfATPb of Fusarium fujikuroi","display_name":"Fluazinam binds with the ATP synthase subunit FfATPh but not with FfATP5 or FfATPb of Fusarium fujikuroi","publication_year":2025,"publication_date":"2025-09-24","ids":{"openalex":"https://openalex.org/W4414459798","doi":"https://doi.org/10.1002/ps.70249","pmid":"https://pubmed.ncbi.nlm.nih.gov/40991829"},"language":"en","primary_location":{"id":"doi:10.1002/ps.70249","doi":"https://doi.org/10.1002/ps.70249","is_oa":false,"landing_page_url":"https://doi.org/10.1002/ps.70249","pdf_url":null,"source":{"id":"https://openalex.org/S31777576","display_name":"Pest Management Science","issn_l":"1526-498X","issn":["1526-498X","1526-4998"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-2","medline","norway-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pest Management Science","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Zelong Peng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119454577","display_name":"Nanjing Agricultural University","ror":"https://ror.org/05td3s095","country_code":"CN","type":"education","lineage":["https://openalex.org/I119454577"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zelong Peng","raw_affiliation_strings":["State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China","State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China","These authors contributed equally"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China","institution_ids":["https://openalex.org/I119454577"]},{"raw_affiliation_string":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China","institution_ids":["https://openalex.org/I119454577"]},{"raw_affiliation_string":"These authors contributed equally","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Weidong Wen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119454577","display_name":"Nanjing Agricultural University","ror":"https://ror.org/05td3s095","country_code":"CN","type":"education","lineage":["https://openalex.org/I119454577"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Weidong Wen","raw_affiliation_strings":["State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China","State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China","These authors contributed equally"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China","institution_ids":["https://openalex.org/I119454577"]},{"raw_affiliation_string":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China","institution_ids":["https://openalex.org/I119454577"]},{"raw_affiliation_string":"These authors contributed equally","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047626379","display_name":"Chongjing Xu","orcid":"https://orcid.org/0000-0002-8182-2625","observed_orcids":["https://orcid.org/0000-0002-8182-2625"]},"institutions":[{"id":"https://openalex.org/I119454577","display_name":"Nanjing Agricultural University","ror":"https://ror.org/05td3s095","country_code":"CN","type":"education","lineage":["https://openalex.org/I119454577"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chongjing Xu","raw_affiliation_strings":["State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China","State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China","institution_ids":["https://openalex.org/I119454577"]},{"raw_affiliation_string":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China","institution_ids":["https://openalex.org/I119454577"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065441101","display_name":"Pengwei Wang","orcid":"https://orcid.org/0000-0001-8882-3447","observed_orcids":["https://orcid.org/0000-0001-8882-3447","https://orcid.org/0000-0002-0362-878X"]},"institutions":[{"id":"https://openalex.org/I119454577","display_name":"Nanjing Agricultural University","ror":"https://ror.org/05td3s095","country_code":"CN","type":"education","lineage":["https://openalex.org/I119454577"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Pengwei Wang","raw_affiliation_strings":["State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China","State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China","institution_ids":["https://openalex.org/I119454577"]},{"raw_affiliation_string":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China","institution_ids":["https://openalex.org/I119454577"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062172696","display_name":"Tingting Ran","orcid":"https://orcid.org/0000-0002-5541-3553","observed_orcids":["https://orcid.org/0000-0002-5541-3553","https://orcid.org/0009-0003-9512-9092"]},"institutions":[{"id":"https://openalex.org/I119454577","display_name":"Nanjing Agricultural University","ror":"https://ror.org/05td3s095","country_code":"CN","type":"education","lineage":["https://openalex.org/I119454577"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Tingting Ran","raw_affiliation_strings":["College of Life Sciences Nanjing Agricultural University  Nanjing China","College of Life Sciences, Nanjing Agricultural University, Nanjing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Life Sciences Nanjing Agricultural University  Nanjing China","institution_ids":["https://openalex.org/I119454577"]},{"raw_affiliation_string":"College of Life Sciences, Nanjing Agricultural University, Nanjing, China","institution_ids":["https://openalex.org/I119454577"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103012319","display_name":"Yiping Hou","orcid":"https://orcid.org/0000-0002-9985-6153","observed_orcids":["https://orcid.org/0000-0002-9985-6153"]},"institutions":[{"id":"https://openalex.org/I119454577","display_name":"Nanjing Agricultural University","ror":"https://ror.org/05td3s095","country_code":"CN","type":"education","lineage":["https://openalex.org/I119454577"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Yiping Hou","raw_affiliation_strings":["State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China","State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China"],"raw_orcid":"https://orcid.org/0000-0002-9985-6153","affiliations":[{"raw_affiliation_string":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China","institution_ids":["https://openalex.org/I119454577"]},{"raw_affiliation_string":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing, China","institution_ids":["https://openalex.org/I119454577"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5062172696","https://openalex.org/A5103012319"],"corresponding_institution_ids":["https://openalex.org/I119454577"],"apc_list":{"value":5360,"currency":"USD","value_usd":5360},"apc_paid":null,"fwci":0.9577,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.77642518,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":96},"biblio":{"volume":"82","issue":"1","first_page":"920","last_page":"927"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11900","display_name":"Fungal Plant Pathogen Control","score":0.7753999829292297,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11900","display_name":"Fungal Plant Pathogen Control","score":0.7753999829292297,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":0.16869999468326569,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12858","display_name":"Plant Disease Resistance and Genetics","score":0.003599999938160181,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluazinam","display_name":"fluazinam","score":0.9990000128746033},{"id":"https://openalex.org/keywords/fusarium-fujikuroi","display_name":"Fusarium fujikuroi","score":0.953000009059906},{"id":"https://openalex.org/keywords/rice-bakanae-disease","display_name":"rice bakanae disease","score":0.9160000085830688},{"id":"https://openalex.org/keywords/binding-affinity","display_name":"binding affinity","score":0.5540000200271606},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.734000027179718},{"id":"https://openalex.org/keywords/microscale-thermophoresis","display_name":"microscale thermophoresis","score":0.8180000185966492},{"id":"https://openalex.org/keywords/molecular-docking","display_name":"molecular docking","score":0.9120000004768372},{"id":"https://openalex.org/keywords/antifungal-mechanism-of-action","display_name":"antifungal mechanism","score":0.652999997138977}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6751000285148621},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.6040999889373779},{"id":"https://openalex.org/C121587506","wikidata":"https://www.wikidata.org/wiki/Q6888113","display_name":"Mode of action","level":2,"score":0.5242999792098999},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5160999894142151},{"id":"https://openalex.org/C2776120743","wikidata":"https://www.wikidata.org/wiki/Q3271540","display_name":"Mechanism of action","level":3,"score":0.48539999127388},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4496000111103058},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3959999978542328},{"id":"https://openalex.org/C112243037","wikidata":"https://www.wikidata.org/wiki/Q22327117","display_name":"ATP synthase","level":3,"score":0.3822000026702881},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.3790999948978424},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.36169999837875366},{"id":"https://openalex.org/C2780791683","wikidata":"https://www.wikidata.org/wiki/Q846785","display_name":"Action (physics)","level":2,"score":0.34150001406669617},{"id":"https://openalex.org/C26517878","wikidata":"https://www.wikidata.org/wiki/Q228039","display_name":"Key (lock)","level":2,"score":0.30809998512268066},{"id":"https://openalex.org/C178910836","wikidata":"https://www.wikidata.org/wiki/Q2196961","display_name":"Molecular model","level":2,"score":0.3027999997138977},{"id":"https://openalex.org/C2776980637","wikidata":"https://www.wikidata.org/wiki/Q427492","display_name":"Enzyme inhibitor","level":3,"score":0.29750001430511475},{"id":"https://openalex.org/C193042331","wikidata":"https://www.wikidata.org/wiki/Q1772003","display_name":"Structure\u2013activity relationship","level":3,"score":0.27639999985694885},{"id":"https://openalex.org/C3020299119","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Binding pocket","level":3,"score":0.275299996137619}],"mesh":[{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005659","descriptor_name":"Fungicides, Industrial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005659","descriptor_name":"Fungicides, Industrial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005659","descriptor_name":"Fungicides, Industrial","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D005659","descriptor_name":"Fungicides, Industrial","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D005670","descriptor_name":"Fusarium","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D005670","descriptor_name":"Fusarium","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D005670","descriptor_name":"Fusarium","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D005670","descriptor_name":"Fusarium","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D005670","descriptor_name":"Fusarium","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005670","descriptor_name":"Fusarium","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005670","descriptor_name":"Fusarium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005670","descriptor_name":"Fusarium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D025261","descriptor_name":"Mitochondrial Proton-Translocating ATPases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D025261","descriptor_name":"Mitochondrial Proton-Translocating ATPases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D025261","descriptor_name":"Mitochondrial Proton-Translocating ATPases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D025261","descriptor_name":"Mitochondrial Proton-Translocating ATPases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D025261","descriptor_name":"Mitochondrial Proton-Translocating ATPases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D025261","descriptor_name":"Mitochondrial Proton-Translocating ATPases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/ps.70249","doi":"https://doi.org/10.1002/ps.70249","is_oa":false,"landing_page_url":"https://doi.org/10.1002/ps.70249","pdf_url":null,"source":{"id":"https://openalex.org/S31777576","display_name":"Pest Management Science","issn_l":"1526-498X","issn":["1526-498X","1526-4998"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-2","medline","norway-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pest Management Science","raw_type":"journal-article"},{"id":"pmid:40991829","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/40991829","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pest management science","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.40450000762939453,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320335777","display_name":"National Key Research and Development Program of China","ror":null}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W1527039050","https://openalex.org/W1967118662","https://openalex.org/W1993321915","https://openalex.org/W2026451326","https://openalex.org/W2041080023","https://openalex.org/W2072535841","https://openalex.org/W2077989537","https://openalex.org/W2091936020","https://openalex.org/W2142444629","https://openalex.org/W2146103350","https://openalex.org/W2190501140","https://openalex.org/W2562200138","https://openalex.org/W2777947468","https://openalex.org/W2790505177","https://openalex.org/W2801374362","https://openalex.org/W2806001393","https://openalex.org/W2890338373","https://openalex.org/W2908816474","https://openalex.org/W2912007564","https://openalex.org/W3096448727","https://openalex.org/W3114271647","https://openalex.org/W4288443361","https://openalex.org/W4321374284","https://openalex.org/W4366293320","https://openalex.org/W4386221499","https://openalex.org/W4386704663","https://openalex.org/W4386885752","https://openalex.org/W4406669171","https://openalex.org/W4409439087"],"related_works":[],"abstract_inverted_index":{"Abstract":[0],"BACKGROUND":[1],"Rice":[2],"bakanae":[3,29],"disease,":[4,30],"a":[5,209,255],"devastating":[6],"disease":[7],"caused":[8],"by":[9],"Fusarium":[10,55],"fujikuroi":[11,56],"infection,":[12],"is":[13,24,69],"primarily":[14],"managed":[15],"through":[16],"chemical":[17],"agents.":[18],"Fluazinam,":[19],"an":[20],"oxidative":[21],"phosphorylation":[22],"uncoupler,":[23],"registered":[25],"for":[26,124,224,231,270],"controlling":[27],"rice":[28],"yet":[31],"its":[32,120,271],"precise":[33,272],"mechanism":[34],"of":[35,46,71,143,175,180,190,219,262,264,278],"action":[36,263],"remains":[37],"unclear.":[38],"Our":[39],"previous":[40],"studies":[41],"found":[42],"that":[43,104,142,154,165,186,240],"deletion":[44],"mutants":[45],"ATP":[47,83],"synthase":[48,84],"subunits":[49,85],"FfATPh,":[50,74,86,244],"FfATP5,":[51,75,87],"and":[52,76,88,95,109,126,133,173,196,226,266],"FfATPb":[53,89],"in":[54,213],"showed":[57,103,164,185],"reduced":[58,199],"sensitivity":[59],"to":[60,65,141,200,208],"fluazinam,":[61],"therefore":[62],"we":[63,238],"aimed":[64],"investigate":[66],"whether":[67],"fluazinam":[68,105,155,166,191,241,265],"capable":[70],"binding":[72,188,250],"with":[73,107,159,171,192,215,243,245],"FfATPb.":[77],"RESULTS":[78],"In":[79,118],"this":[80],"study,":[81],"the":[82,110,144,187,260,268],"were":[90,128],"obtained":[91],"via":[92],"prokaryotic":[93],"expression":[94],"nickel\u2010column":[96],"affinity":[97,158,178,189],"purification.":[98],"Microscale":[99],"thermophoresis":[100],"(MST)":[101],"results":[102],"binds":[106,242],"His9\u2010MBP\u2010TEV\u2010ATPh":[108],"K":[111,121,216],"d":[112,122,217],"was":[113,198],"25.34":[114],"\u00b1":[115,130,135,148,221,228],"11.71":[116],"nM.":[117],"contrast,":[119],"values":[123,218],"His9\u2010MBP\u2010TEV\u2010ATP5":[125],"His9\u2010MBP\u2010TEV\u2010ATPb":[127],"1.49":[129],"1.32":[131],"\u03bcM":[132,230],"1.04":[134],"0.75":[136],"\u03bcM,":[137],"respectively,":[138],"both":[139],"similar":[140],"His9\u2010MBP\u2010TEV":[145],"control":[146],"(2.57":[147],"2.02":[149],"\u03bcM).":[150],"These":[151,252],"findings":[152,253],"indicate":[153],"exhibits":[156],"specific":[157],"FfATPh.":[160,176],"Molecular":[161],"docking":[162],"analysis":[163],"can":[167],"form":[168],"hydrogen":[169],"bonds":[170],"Asp42":[172,206,246],"Phe43":[174],"Further":[177],"measurements":[179],"purified":[181],"FfATPh":[182],"point\u2010mutant":[183],"proteins":[184],"His9\u2010MBP\u2010TEV\u2010ATPh\u2010D42A,":[193],"D42H,":[194],"F43A,":[195],"F43S":[197],"varying":[201],"degrees.":[202],"Mutations":[203],"at":[204],"residue":[205],"led":[207],"more":[210],"significant":[211],"decrease":[212],"affinity,":[214],"639":[220],"431":[222],"nM":[223],"D42A":[225],"1.61":[227],"1.36":[229],"D42H.":[232],"CONCLUSION":[233],"Based":[234],"on":[235,259],"these":[236],"data,":[237],"speculate":[239],"serving":[247],"as":[248],"key":[249],"sites.":[251],"provide":[254],"new":[256],"molecular":[257],"perspective":[258],"mode":[261],"lay":[267],"foundation":[269],"structural":[273],"optimization.":[274],"\u00a9":[275],"2025":[276],"Society":[277],"Chemical":[279],"Industry.":[280]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2025-09-24T00:00:00"}