{"id":"https://openalex.org/W4414312955","doi":"https://doi.org/10.1016/j.humimm.2025.111530","title":"Optimizing adsorption elution assays: why antibody saturation matters","display_name":"Optimizing adsorption elution assays: why antibody saturation matters","publication_year":2025,"publication_date":"2025-09-01","ids":{"openalex":"https://openalex.org/W4414312955","doi":"https://doi.org/10.1016/j.humimm.2025.111530"},"language":"en","primary_location":{"id":"doi:10.1016/j.humimm.2025.111530","doi":"https://doi.org/10.1016/j.humimm.2025.111530","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.humimm.2025.111530","pdf_url":null,"source":{"id":"https://openalex.org/S6860681","display_name":"Human Immunology","issn_l":"0198-8859","issn":["0198-8859","1879-1166"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Human Immunology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"D. Austin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"D. Austin","raw_affiliation_strings":["Transplant Immunology, Northwestern University, Chicago, IL, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Transplant Immunology, Northwestern University, Chicago, IL, United States","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"middle","author":{"id":null,"display_name":"C. Maguire","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. Maguire","raw_affiliation_strings":["Transplant Immunology, Northwestern University, Chicago, IL, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Transplant Immunology, Northwestern University, Chicago, IL, United States","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067260564","display_name":"Anat R. Tambur","orcid":"https://orcid.org/0000-0002-0320-9825","observed_orcids":["https://orcid.org/0000-0002-0320-9825"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A. Tambur","raw_affiliation_strings":["Transplant Immunology, Northwestern University, Chicago, IL, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Transplant Immunology, Northwestern University, Chicago, IL, United States","institution_ids":["https://openalex.org/I111979921"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I111979921"],"apc_list":{"value":3600,"currency":"USD","value_usd":3600},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.24324835,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"86","issue":null,"first_page":"111530","last_page":"111530"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.8773999810218811,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.8773999810218811,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.06379999965429306,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.01269999984651804,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/antibody-saturation","display_name":"antibody saturation","score":0.9729999899864197},{"id":"https://openalex.org/keywords/antibody-affinity","display_name":"antibody affinity","score":0.3889999985694885},{"id":"https://openalex.org/keywords/antibody-antigen-interaction","display_name":"antigen-antibody reaction","score":0.6240000128746033},{"id":"https://openalex.org/keywords/antibody-titer-measurement","display_name":"antibody titer measurement","score":0.30799999833106995},{"id":"https://openalex.org/keywords/assay-optimization","display_name":"assay optimization","score":0.4169999957084656}],"concepts":[{"id":"https://openalex.org/C73826308","wikidata":"https://www.wikidata.org/wiki/Q684907","display_name":"Elution","level":2,"score":0.775600016117096},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7405999898910522},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.7060999870300293},{"id":"https://openalex.org/C9930424","wikidata":"https://www.wikidata.org/wiki/Q7426587","display_name":"Saturation (graph theory)","level":2,"score":0.6646999716758728},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6614999771118164},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.3246000111103058},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.28949999809265137},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.22589999437332153},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.17270000278949738},{"id":"https://openalex.org/C42360764","wikidata":"https://www.wikidata.org/wiki/Q83588","display_name":"Chemical engineering","level":1,"score":0.17139999568462372}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1016/j.humimm.2025.111530","doi":"https://doi.org/10.1016/j.humimm.2025.111530","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.humimm.2025.111530","pdf_url":null,"source":{"id":"https://openalex.org/S6860681","display_name":"Human Immunology","issn_l":"0198-8859","issn":["0198-8859","1879-1166"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Human Immunology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W6942397712","https://openalex.org/W1969833891","https://openalex.org/W4210717713","https://openalex.org/W4246158694","https://openalex.org/W2056722396","https://openalex.org/W4249551714","https://openalex.org/W4245801892","https://openalex.org/W7004954323","https://openalex.org/W2979526153","https://openalex.org/W2405257765"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2025-10-10T00:00:00"}