{"id":"https://openalex.org/W4414112409","doi":"https://doi.org/10.1007/s12033-025-01500-x","title":"Expression, Purification and Biochemical Characterisation of Babesia bigemina Lactate Dehydrogenase","display_name":"Expression, Purification and Biochemical Characterisation of Babesia bigemina Lactate Dehydrogenase","publication_year":2025,"publication_date":"2025-09-10","ids":{"openalex":"https://openalex.org/W4414112409","doi":"https://doi.org/10.1007/s12033-025-01500-x","pmid":"https://pubmed.ncbi.nlm.nih.gov/40931271"},"language":"en","primary_location":{"id":"doi:10.1007/s12033-025-01500-x","doi":"https://doi.org/10.1007/s12033-025-01500-x","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s12033-025-01500-x","pdf_url":null,"source":{"id":"https://openalex.org/S31191957","display_name":"Molecular Biotechnology","issn_l":"1073-6085","issn":["1073-6085","1559-0305"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5013320809","display_name":"Safiye Merve Bostancioglu","orcid":"https://orcid.org/0000-0002-0239-3966","observed_orcids":["https://orcid.org/0000-0002-0239-3966"]},"institutions":[{"id":"https://openalex.org/I74897591","display_name":"Marmara University","ror":"https://ror.org/02kswqa67","country_code":"TR","type":"education","lineage":["https://openalex.org/I74897591"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Safiye Merve Bostancioglu","raw_affiliation_strings":["Department of Biology, Faculty of Science, Marmara University, G\u00f6ztepe, 34722, Istanbul, T\u00fcrkiye"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Faculty of Science, Marmara University, G\u00f6ztepe, 34722, Istanbul, T\u00fcrkiye","institution_ids":["https://openalex.org/I74897591"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035730849","display_name":"\u00d6zkan Dan\u0131\u015f","orcid":"https://orcid.org/0000-0003-1781-0520","observed_orcids":["https://orcid.org/0000-0003-1781-0520"]},"institutions":[{"id":"https://openalex.org/I74897591","display_name":"Marmara University","ror":"https://ror.org/02kswqa67","country_code":"TR","type":"education","lineage":["https://openalex.org/I74897591"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Ozkan Danis","raw_affiliation_strings":["Department of Chemistry, Faculty of Science, Marmara University, G\u00f6ztepe, 34722, Istanbul, T\u00fcrkiye"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Faculty of Science, Marmara University, G\u00f6ztepe, 34722, Istanbul, T\u00fcrkiye","institution_ids":["https://openalex.org/I74897591"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003741402","display_name":"Sinem Ko\u00e7er","orcid":"https://orcid.org/0000-0003-0517-7422","observed_orcids":["https://orcid.org/0000-0003-0517-7422"]},"institutions":[{"id":"https://openalex.org/I311431067","display_name":"Istanbul Yeni Y\u00fczy\u0131l University","ror":"https://ror.org/04z33a802","country_code":"TR","type":"education","lineage":["https://openalex.org/I311431067"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Sinem Kocer","raw_affiliation_strings":["Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, 34010, Istanbul, T\u00fcrkiye"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, 34010, Istanbul, T\u00fcrkiye","institution_ids":["https://openalex.org/I311431067"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5020668039","display_name":"\u00d6zal Mutlu","orcid":"https://orcid.org/0000-0003-4551-5780","observed_orcids":["https://orcid.org/0000-0003-4551-5780"]},"institutions":[{"id":"https://openalex.org/I74897591","display_name":"Marmara University","ror":"https://ror.org/02kswqa67","country_code":"TR","type":"education","lineage":["https://openalex.org/I74897591"]}],"countries":["TR"],"is_corresponding":true,"raw_author_name":"Ozal Mutlu","raw_affiliation_strings":["Department of Biology, Faculty of Science, Marmara University, G\u00f6ztepe, 34722, Istanbul, T\u00fcrkiye. ozal.mutlu@marmara.edu.tr","Department of Biology, Faculty of Science, Marmara University, G\u00f6ztepe, 34722, Istanbul, T\u00fcrkiye"],"raw_orcid":"https://orcid.org/0000-0003-4551-5780","affiliations":[{"raw_affiliation_string":"Department of Biology, Faculty of Science, Marmara University, G\u00f6ztepe, 34722, Istanbul, T\u00fcrkiye. ozal.mutlu@marmara.edu.tr","institution_ids":["https://openalex.org/I74897591"]},{"raw_affiliation_string":"Department of Biology, Faculty of Science, Marmara University, G\u00f6ztepe, 34722, Istanbul, T\u00fcrkiye","institution_ids":["https://openalex.org/I74897591"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5020668039"],"corresponding_institution_ids":["https://openalex.org/I74897591"],"apc_list":{"value":3790,"currency":"USD","value_usd":3790},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.22328595,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"68","issue":"5","first_page":"2410","last_page":"2421"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10890","display_name":"Trypanosoma species research and implications","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/babesia-bigemina","display_name":"Babesia bigemina","score":1.0},{"id":"https://openalex.org/keywords/lactate-dehydrogenase","display_name":"lactate dehydrogenase","score":1.0},{"id":"https://openalex.org/keywords/bovine-babesiosis","display_name":"bovine babesiosis","score":0.9779999852180481},{"id":"https://openalex.org/keywords/tick-borne-protozoan-parasites","display_name":"tick-borne protozoan parasites","score":0.7390000224113464},{"id":"https://openalex.org/keywords/anaerobic-glycolysis","display_name":"anaerobic glycolysis","score":0.8429999947547913},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.7200000286102295},{"id":"https://openalex.org/keywords/allosteric-enzymes","display_name":"allosteric enzymes","score":0.7799999713897705},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.7960000038146973},{"id":"https://openalex.org/keywords/optimal-ph","display_name":"pH optimum","score":0.7919999957084656},{"id":"https://openalex.org/keywords/pyruvate","display_name":"pyruvate","score":0.7699999809265137},{"id":"https://openalex.org/keywords/nadh","display_name":"NADH","score":0.9950000047683716},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.5460000038146973}],"concepts":[{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7269999980926514},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.7059999704360962},{"id":"https://openalex.org/C2777318727","wikidata":"https://www.wikidata.org/wiki/Q409464","display_name":"Lactate dehydrogenase","level":3,"score":0.6503000259399414},{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.6003999710083008},{"id":"https://openalex.org/C20251656","wikidata":"https://www.wikidata.org/wiki/Q162643","display_name":"Glycolysis","level":3,"score":0.5795000195503235},{"id":"https://openalex.org/C2910449918","wikidata":"https://www.wikidata.org/wiki/Q797641","display_name":"Babesia bigemina","level":3,"score":0.5724999904632568},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5285000205039978},{"id":"https://openalex.org/C28212737","wikidata":"https://www.wikidata.org/wiki/Q5381543","display_name":"Enzyme assay","level":3,"score":0.45339998602867126},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.42669999599456787},{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.41449999809265137},{"id":"https://openalex.org/C165135838","wikidata":"https://www.wikidata.org/wiki/Q133895","display_name":"Citric acid cycle","level":3,"score":0.3465000092983246},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.3346000015735626},{"id":"https://openalex.org/C125209161","wikidata":"https://www.wikidata.org/wiki/Q422816","display_name":"Enzyme activator","level":3,"score":0.30390000343322754},{"id":"https://openalex.org/C2777795018","wikidata":"https://www.wikidata.org/wiki/Q134734","display_name":"Cryptosporidium parvum","level":2,"score":0.3021000027656555},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.30079999566078186},{"id":"https://openalex.org/C198684833","wikidata":"https://www.wikidata.org/wiki/Q2823748","display_name":"Specific activity","level":3,"score":0.29750001430511475},{"id":"https://openalex.org/C192989942","wikidata":"https://www.wikidata.org/wiki/Q68685","display_name":"Metabolic pathway","level":3,"score":0.29280000925064087},{"id":"https://openalex.org/C5140985","wikidata":"https://www.wikidata.org/wiki/Q483866","display_name":"Anaerobic exercise","level":2,"score":0.29269999265670776},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.29170000553131104},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.2867000102996826},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.2777000069618225},{"id":"https://openalex.org/C188618488","wikidata":"https://www.wikidata.org/wiki/Q24776639","display_name":"Enolase","level":3,"score":0.2669999897480011},{"id":"https://openalex.org/C88099638","wikidata":"https://www.wikidata.org/wiki/Q412856","display_name":"Pyruvate dehydrogenase complex","level":3,"score":0.2590999901294708},{"id":"https://openalex.org/C58431802","wikidata":"https://www.wikidata.org/wiki/Q420663","display_name":"Phosphofructokinase 2","level":3,"score":0.2554999887943268}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001403","descriptor_name":"Babesia","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001403","descriptor_name":"Babesia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007770","descriptor_name":"L-Lactate Dehydrogenase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007770","descriptor_name":"L-Lactate Dehydrogenase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D007770","descriptor_name":"L-Lactate Dehydrogenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D007770","descriptor_name":"L-Lactate Dehydrogenase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D009243","descriptor_name":"NAD","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1007/s12033-025-01500-x","doi":"https://doi.org/10.1007/s12033-025-01500-x","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s12033-025-01500-x","pdf_url":null,"source":{"id":"https://openalex.org/S31191957","display_name":"Molecular Biotechnology","issn_l":"1073-6085","issn":["1073-6085","1559-0305"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Biotechnology","raw_type":"journal-article"},{"id":"pmid:40931271","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/40931271","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular biotechnology","raw_type":"Journal Article"},{"id":"pmh:5ce4cabd-eece-4fb9-85f1-b6bfbd7d0181","doi":null,"is_oa":false,"landing_page_url":"https://avesis.yeniyuzyil.edu.tr/publication/details/5ce4cabd-eece-4fb9-85f1-b6bfbd7d0181/oai","pdf_url":null,"source":{"id":"https://openalex.org/S7407055138","display_name":"\u0130stanbul Yeni Y\u00fczy\u0131l \u00dcniversitesi Research Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3525862961","display_name":null,"funder_award_id":"FDK-2022-10061","funder_id":"https://openalex.org/F4320325078","funder_display_name":"Marmara \u00dcniversitesi"}],"funders":[{"id":"https://openalex.org/F4320325078","display_name":"Marmara \u00dcniversitesi","ror":"https://ror.org/02kswqa67"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":41,"referenced_works":["https://openalex.org/W1551921975","https://openalex.org/W1983691075","https://openalex.org/W1985629904","https://openalex.org/W1986180717","https://openalex.org/W1988790695","https://openalex.org/W1991901574","https://openalex.org/W1997480152","https://openalex.org/W2000440464","https://openalex.org/W2002470519","https://openalex.org/W2014637255","https://openalex.org/W2020471029","https://openalex.org/W2026136751","https://openalex.org/W2032308437","https://openalex.org/W2045589999","https://openalex.org/W2059054679","https://openalex.org/W2059391470","https://openalex.org/W2070190434","https://openalex.org/W2102028093","https://openalex.org/W2111869141","https://openalex.org/W2115072810","https://openalex.org/W2128635872","https://openalex.org/W2142995586","https://openalex.org/W2146931552","https://openalex.org/W2152185415","https://openalex.org/W2161703382","https://openalex.org/W2179602398","https://openalex.org/W2202378115","https://openalex.org/W2283905972","https://openalex.org/W2337741944","https://openalex.org/W2945699469","https://openalex.org/W2977910016","https://openalex.org/W2990973954","https://openalex.org/W3129520255","https://openalex.org/W4205146782","https://openalex.org/W4205161636","https://openalex.org/W4223534559","https://openalex.org/W4224286493","https://openalex.org/W4303423775","https://openalex.org/W4308616064","https://openalex.org/W4394694796","https://openalex.org/W4406115356"],"related_works":["https://openalex.org/W2056970828","https://openalex.org/W2082416493","https://openalex.org/W2054120105","https://openalex.org/W4205146782","https://openalex.org/W2372924859","https://openalex.org/W2055997333","https://openalex.org/W7100707122","https://openalex.org/W2086302599","https://openalex.org/W4362671277","https://openalex.org/W3100394358"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2025-10-10T00:00:00"}