{"id":"https://openalex.org/W4413805340","doi":"https://doi.org/10.14719/pst.9312","title":"Intron hairpin RNA derived from the HC-Pro gene confers resistance to papaya ringspot virus in transgenic Nicotiana benthamiana","display_name":"Intron hairpin RNA derived from the HC-Pro gene confers resistance to papaya ringspot virus in transgenic Nicotiana benthamiana","publication_year":2025,"publication_date":"2025-08-29","ids":{"openalex":"https://openalex.org/W4413805340","doi":"https://doi.org/10.14719/pst.9312"},"language":"en","primary_location":{"id":"doi:10.14719/pst.9312","doi":"https://doi.org/10.14719/pst.9312","is_oa":true,"landing_page_url":"https://doi.org/10.14719/pst.9312","pdf_url":"https://horizonepublishing.com/journals/index.php/PST/article/download/9312/9732","source":{"id":"https://openalex.org/S4210213485","display_name":"Plant Science Today","issn_l":"2348-1900","issn":["2348-1900"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Science Today","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://horizonepublishing.com/journals/index.php/PST/article/download/9312/9732","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5119468395","display_name":"R T Kheta","orcid":"https://orcid.org/0009-0002-7689-3987","observed_orcids":["https://orcid.org/0009-0002-7689-3987"]},"institutions":[{"id":"https://openalex.org/I199921877","display_name":"University of Agricultural Sciences, Bangalore","ror":"https://ror.org/03js6zg56","country_code":"IN","type":"education","lineage":["https://openalex.org/I199921877"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"R T Kheta","raw_affiliation_strings":["Department of Plant Biotechnology, University of Agricultural Sciences, Gandhi Krishi Vigyana Kendra, Bangalore 560 065, Karnataka, India"],"raw_orcid":"https://orcid.org/0009-0002-7689-3987","affiliations":[{"raw_affiliation_string":"Department of Plant Biotechnology, University of Agricultural Sciences, Gandhi Krishi Vigyana Kendra, Bangalore 560 065, Karnataka, India","institution_ids":["https://openalex.org/I199921877"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018409826","display_name":"Anitha Peter","orcid":"https://orcid.org/0000-0002-7872-8574","observed_orcids":["https://orcid.org/0000-0002-7872-8574"]},"institutions":[{"id":"https://openalex.org/I199921877","display_name":"University of Agricultural Sciences, Bangalore","ror":"https://ror.org/03js6zg56","country_code":"IN","type":"education","lineage":["https://openalex.org/I199921877"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"P Anitha","raw_affiliation_strings":["Department of Plant Biotechnology, University of Agricultural Sciences, Gandhi Krishi Vigyana Kendra, Bangalore 560 065, Karnataka, India"],"raw_orcid":"https://orcid.org/0000-0002-7872-8574","affiliations":[{"raw_affiliation_string":"Department of Plant Biotechnology, University of Agricultural Sciences, Gandhi Krishi Vigyana Kendra, Bangalore 560 065, Karnataka, India","institution_ids":["https://openalex.org/I199921877"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5085836284","display_name":"V. Deepika","orcid":"https://orcid.org/0009-0002-0139-4892","observed_orcids":["https://orcid.org/0009-0002-0139-4892"]},"institutions":[{"id":"https://openalex.org/I199921877","display_name":"University of Agricultural Sciences, Bangalore","ror":"https://ror.org/03js6zg56","country_code":"IN","type":"education","lineage":["https://openalex.org/I199921877"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"V Deepika","raw_affiliation_strings":["Department of Plant Biotechnology, University of Agricultural Sciences, Gandhi Krishi Vigyana Kendra, Bangalore 560 065, Karnataka, India"],"raw_orcid":"https://orcid.org/0009-0002-0139-4892","affiliations":[{"raw_affiliation_string":"Department of Plant Biotechnology, University of Agricultural Sciences, Gandhi Krishi Vigyana Kendra, Bangalore 560 065, Karnataka, India","institution_ids":["https://openalex.org/I199921877"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I199921877"],"apc_list":null,"apc_paid":null,"fwci":0.5113,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.67354266,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":96},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11075","display_name":"Insect Resistance and Genetics","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/papaya-ringspot-virus","display_name":"papaya ringspot virus","score":0.8450000286102295},{"id":"https://openalex.org/keywords/virus-resistance","display_name":"virus resistance","score":0.6460000276565552},{"id":"https://openalex.org/keywords/virus-induced-gene-silencing","display_name":"virus-induced gene silencing","score":0.828000009059906},{"id":"https://openalex.org/keywords/sirna","display_name":"siRNA","score":0.546999990940094},{"id":"https://openalex.org/keywords/nicotiana-benthamiana","display_name":"Nicotiana benthamiana","score":0.8870000243186951},{"id":"https://openalex.org/keywords/genetically-modified-plants","display_name":"transgenic plants","score":0.9010000228881836},{"id":"https://openalex.org/keywords/agrobacterium-mediated-transformation","display_name":"Agrobacterium-mediated transformation","score":0.7350000143051147},{"id":"https://openalex.org/keywords/plant-virus-resistance","display_name":"plant virus resistance","score":0.3319999873638153}],"concepts":[{"id":"https://openalex.org/C2780562374","wikidata":"https://www.wikidata.org/wiki/Q3024956","display_name":"Nicotiana benthamiana","level":3,"score":0.8777275085449219},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7775084972381592},{"id":"https://openalex.org/C2779656422","wikidata":"https://www.wikidata.org/wiki/Q2700446","display_name":"Agrobacterium","level":4,"score":0.7243324518203735},{"id":"https://openalex.org/C190232843","wikidata":"https://www.wikidata.org/wiki/Q1950808","display_name":"Small hairpin RNA","level":4,"score":0.6843031644821167},{"id":"https://openalex.org/C2779117320","wikidata":"https://www.wikidata.org/wiki/Q7132538","display_name":"Papaya ringspot virus","level":5,"score":0.5870225429534912},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4947035014629364},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.4843508005142212},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.47619497776031494},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4749479591846466},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.45368823409080505},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.36967235803604126},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.36138367652893066},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3319907784461975},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.30981457233428955},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.28036004304885864},{"id":"https://openalex.org/C2994092193","wikidata":"https://www.wikidata.org/wiki/Q16769446","display_name":"Coat protein","level":4,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.14719/pst.9312","doi":"https://doi.org/10.14719/pst.9312","is_oa":true,"landing_page_url":"https://doi.org/10.14719/pst.9312","pdf_url":"https://horizonepublishing.com/journals/index.php/PST/article/download/9312/9732","source":{"id":"https://openalex.org/S4210213485","display_name":"Plant Science Today","issn_l":"2348-1900","issn":["2348-1900"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Science Today","raw_type":"journal-article"},{"id":"pmh:oai:ojs.horizonepublishing.com:article/9312","doi":null,"is_oa":true,"landing_page_url":"https://horizonepublishing.com/journals/index.php/PST/article/view/9312","pdf_url":null,"source":{"id":"https://openalex.org/S4210213485","display_name":"Plant Science Today","issn_l":"2348-1900","issn":["2348-1900"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"10.14719/pst.2025.12.4","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.14719/pst.9312","doi":"https://doi.org/10.14719/pst.9312","is_oa":true,"landing_page_url":"https://doi.org/10.14719/pst.9312","pdf_url":"https://horizonepublishing.com/journals/index.php/PST/article/download/9312/9732","source":{"id":"https://openalex.org/S4210213485","display_name":"Plant Science Today","issn_l":"2348-1900","issn":["2348-1900"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Science Today","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.9208999872207642}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320717","display_name":"Department of Biotechnology, Ministry of Science and Technology, India","ror":"https://ror.org/03tjsyq23"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4413805340.pdf","grobid_xml":"https://content.openalex.org/works/W4413805340.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2803153159","https://openalex.org/W1996595592","https://openalex.org/W3041659780","https://openalex.org/W4391387446","https://openalex.org/W4393069141","https://openalex.org/W4413805340","https://openalex.org/W2789456085","https://openalex.org/W2357889036","https://openalex.org/W2373697899","https://openalex.org/W2067340319"],"abstract_inverted_index":{"In":[0],"the":[1,19,22,59,84,104,110,134,151,170],"present":[2],"study,":[3],"cDNA":[4],"from":[5],"a":[6,44,51,128],"papaya":[7],"leaf":[8],"sample":[9],"infected":[10],"with":[11,148],"Papaya":[12],"Ringspot":[13],"Virus":[14,68],"(PRSV)":[15],"was":[16,40,99,157,167,217],"used":[17,41],"for":[18,159,165,181],"amplification":[20,162],"of":[21,31,38,87,133,143,175,204],"HC-Pro":[23,39,98,161],"gene,":[24],"which":[25,49],"yielded":[26],"an":[27],"expected":[28],"amplicon":[29],"size":[30],"~1350":[32],"bp.":[33],"The":[34,64,92],"partial":[35],"coding":[36],"sequence":[37,73],"to":[42,169],"design":[43],"hairpin":[45,95],"RNA":[46,96],"(hpRNA)":[47],"construct,":[48],"included":[50],"cowpea":[52],"spliceosomal":[53],"intron":[54,94],"(148":[55],"bp)":[56],"inserted":[57],"between":[58],"hpRNA":[60],"arms":[61],"(ihpRNA":[62],"HC-Pro).":[63],"potential":[65],"siRNAs":[66],"and":[67,81,101,109,163,193,208],"Induced":[69],"Gene":[70],"Silencing":[71],"(VIGS)":[72],"predicted":[74],"by":[75,123,189],"integrated":[76],"bioinformatics":[77],"tools":[78],"(siDirect":[79],"2.0":[80],"pssRNAit)":[82],"identified":[83],"best":[85],"region":[86],"201":[88],"bp":[89],"(557-757":[90],"bp).":[91],"designed":[93],"(ihpRNA)":[97],"synthesized":[100],"cloned":[102],"into":[103,118],"plant":[105],"expression":[106,195],"vector":[107],"(pBI121)":[108],"resulting":[111],"recombinant":[112],"plasmids":[113],"(pBI121::CaMV35s::ihpRNA-HC-Pro::NOS)":[114],"were":[115,146,179,187],"subsequently":[116],"transformed":[117],"Agrobacterium":[119,149],"tumefaciens":[120],"(LBA4404)":[121],"followed":[122],"co-cultivation":[124],"in":[125,150,197],"N.":[126],"benthamiana,":[127],"model":[129],"plant.":[130],"Molecular":[131],"confirmation":[132],"T0":[135],"lines":[136,178],"via":[137],"PCR":[138],"indicated":[139],"that":[140,156,214],"three":[141],"out":[142,174],"four":[144],"plants":[145,200],"contaminated":[147],"apoplast.":[152],"A":[153],"single":[154],"line":[155],"positive":[158],"ihpRNA":[160,182],"negative":[164],"VirG,":[166],"forwarded":[168],"T1":[171,177,198],"generation.":[172],"Six":[173],"ten":[176],"amplified":[180],"HC-Pro.":[183],"Single":[184],"copy":[185],"insertions":[186],"confirmed":[188],"southern":[190],"blotting.":[191],"Bioassay":[192],"gene":[194,222],"tests":[196],"transgenic":[199],"showed":[201],"lower":[202],"levels":[203],"NbPOD,":[205],"NbAPX,":[206],"NbCAT":[207],"HcPro,":[209],"like":[210],"healthy":[211],"plants,":[212],"suggesting":[213],"PRSV":[215],"resistance":[216],"successfully":[218],"achieved":[219],"through":[220],"ihpRNA-mediated":[221],"silencing.":[223]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-09-21T07:43:02.447757","created_date":"2025-10-10T00:00:00"}