{"id":"https://openalex.org/W4413482014","doi":"https://doi.org/10.5534/wjmh.250110","title":"Development of a Noble On-Site Dual Screening System for Male Fertility of Sperm Count and Hyaluronidase Activity","display_name":"Development of a Noble On-Site Dual Screening System for Male Fertility of Sperm Count and Hyaluronidase Activity","publication_year":2025,"publication_date":"2025-08-25","ids":{"openalex":"https://openalex.org/W4413482014","doi":"https://doi.org/10.5534/wjmh.250110","pmid":"https://pubmed.ncbi.nlm.nih.gov/40878121","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13302969"},"language":"en","primary_location":{"id":"doi:10.5534/wjmh.250110","doi":"https://doi.org/10.5534/wjmh.250110","is_oa":true,"landing_page_url":"https://doi.org/10.5534/wjmh.250110","pdf_url":"http://wjmh.org/Synapse/Data/PDFData/2074WJMH/wjmh-43-e64.pdf","source":{"id":"https://openalex.org/S2764675658","display_name":"The World Journal of Men s Health","issn_l":"2287-4208","issn":["2287-4208","2287-4690"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","kci-registered","ki-jl-1","norway-1","oarl","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319420","host_organization_name":"Korean Society for Sexual Medicine and Andrology","host_organization_lineage":["https://openalex.org/P4310319420"],"host_organization_lineage_names":["Korean Society for Sexual Medicine and Andrology"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The World Journal of Men's Health","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"http://wjmh.org/Synapse/Data/PDFData/2074WJMH/wjmh-43-e64.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113329061","display_name":"Jisuk Lee","orcid":"https://orcid.org/0009-0007-7105-8743","observed_orcids":["https://orcid.org/0009-0007-7105-8743"]},"institutions":[{"id":"https://openalex.org/I21028721","display_name":"Eulji University","ror":"https://ror.org/005bty106","country_code":"KR","type":"education","lineage":["https://openalex.org/I21028721"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Jisuk Lee","raw_affiliation_strings":["Department of Senior Healthcare, Eulji University Graduated School, Seongnam, Korea"],"raw_orcid":"https://orcid.org/0009-0007-7105-8743","affiliations":[{"raw_affiliation_string":"Department of Senior Healthcare, Eulji University Graduated School, Seongnam, Korea","institution_ids":["https://openalex.org/I21028721"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004611645","display_name":"Seung\u2010Hun Song","orcid":"https://orcid.org/0000-0003-4649-9129","observed_orcids":["https://orcid.org/0000-0003-4649-9129"]},"institutions":[{"id":"https://openalex.org/I4210148737","display_name":"CHA University","ror":"https://ror.org/04yka3j04","country_code":"KR","type":"education","lineage":["https://openalex.org/I4210148737","https://openalex.org/I4405258985"]},{"id":"https://openalex.org/I4210165484","display_name":"CHA University Gangnam Medical Center","ror":"https://ror.org/05rtdxx03","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I4210148737","https://openalex.org/I4210165484","https://openalex.org/I4405258985"]},{"id":"https://openalex.org/I4405258721","display_name":"CHA University Fertility Center","ror":"https://ror.org/013x1r993","country_code":"KR","type":"healthcare","lineage":["https://openalex.org/I4210134376","https://openalex.org/I4210148737","https://openalex.org/I4405258721","https://openalex.org/I4405258985"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Seung-Hun Song","raw_affiliation_strings":["Department of Urology, Fertility Center, CHA Gangnam Medical Center, CHA University, Seoul, Korea"],"raw_orcid":"https://orcid.org/0000-0003-4649-9129","affiliations":[{"raw_affiliation_string":"Department of Urology, Fertility Center, CHA Gangnam Medical Center, CHA University, Seoul, Korea","institution_ids":["https://openalex.org/I4210148737","https://openalex.org/I4210165484","https://openalex.org/I4405258721"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014289143","display_name":"Jin Hyun Jun","orcid":"https://orcid.org/0000-0001-9898-4471","observed_orcids":["https://orcid.org/0000-0001-9898-4471"]},"institutions":[{"id":"https://openalex.org/I21028721","display_name":"Eulji University","ror":"https://ror.org/005bty106","country_code":"KR","type":"education","lineage":["https://openalex.org/I21028721"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Jin Hyun Jun","raw_affiliation_strings":["Department of Biomedical Laboratory Science, College of Health Science, Eulji University, Seongnam, Korea","Department of Senior Healthcare, Eulji University Graduated School, Seongnam, Korea"],"raw_orcid":"https://orcid.org/0000-0001-9898-4471","affiliations":[{"raw_affiliation_string":"Department of Biomedical Laboratory Science, College of Health Science, Eulji University, Seongnam, Korea","institution_ids":["https://openalex.org/I21028721"]},{"raw_affiliation_string":"Department of Senior Healthcare, Eulji University Graduated School, Seongnam, Korea","institution_ids":["https://openalex.org/I21028721"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077132260","display_name":"Il\u2010Hoon Cho","orcid":"https://orcid.org/0000-0002-4991-2268","observed_orcids":["https://orcid.org/0000-0002-4991-2268"]},"institutions":[{"id":"https://openalex.org/I21028721","display_name":"Eulji University","ror":"https://ror.org/005bty106","country_code":"KR","type":"education","lineage":["https://openalex.org/I21028721"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Il-Hoon Cho","raw_affiliation_strings":["Department of Biomedical Laboratory Science, College of Health Science, Eulji University, Seongnam, Korea","Department of Senior Healthcare, Eulji University Graduated School, Seongnam, Korea"],"raw_orcid":"https://orcid.org/0000-0002-4991-2268","affiliations":[{"raw_affiliation_string":"Department of Biomedical Laboratory Science, College of Health Science, Eulji University, Seongnam, Korea","institution_ids":["https://openalex.org/I21028721"]},{"raw_affiliation_string":"Department of Senior Healthcare, Eulji University Graduated School, Seongnam, Korea","institution_ids":["https://openalex.org/I21028721"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5014289143","https://openalex.org/A5077132260"],"corresponding_institution_ids":["https://openalex.org/I21028721"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"44","issue":"3","first_page":"669","last_page":"669"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9442999958992004,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.9442999958992004,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.027400000020861626,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11156","display_name":"Reproductive Health and Technologies","score":0.0034000000450760126,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/male-infertility","display_name":"male infertility","score":0.7139999866485596},{"id":"https://openalex.org/keywords/sperm-count","display_name":"sperm count","score":0.9240000247955322},{"id":"https://openalex.org/keywords/hyaluronidase-activity","display_name":"hyaluronidase activity","score":0.9810000061988831},{"id":"https://openalex.org/keywords/lateral-flow-assay","display_name":"lateral flow assay","score":0.7940000295639038},{"id":"https://openalex.org/keywords/on-site-screening","display_name":"on-site screening","score":0.7850000262260437},{"id":"https://openalex.org/keywords/oligospermia","display_name":"oligozoospermia","score":0.9169999957084656},{"id":"https://openalex.org/keywords/azoospermia","display_name":"azoospermia","score":0.9850000143051147},{"id":"https://openalex.org/keywords/home-testing","display_name":"home testing","score":0.42500001192092896}],"concepts":[{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.855053186416626},{"id":"https://openalex.org/C518429986","wikidata":"https://www.wikidata.org/wiki/Q964401","display_name":"Fertility","level":3,"score":0.6556482911109924},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.5289911031723022},{"id":"https://openalex.org/C2780980858","wikidata":"https://www.wikidata.org/wiki/Q110022","display_name":"Dual (grammatical number)","level":2,"score":0.4661145806312561},{"id":"https://openalex.org/C29456083","wikidata":"https://www.wikidata.org/wiki/Q1221899","display_name":"Gynecology","level":1,"score":0.41623297333717346},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.31169378757476807},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.14288216829299927},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.11111930012702942},{"id":"https://openalex.org/C124952713","wikidata":"https://www.wikidata.org/wiki/Q8242","display_name":"Literature","level":1,"score":0.0},{"id":"https://openalex.org/C142362112","wikidata":"https://www.wikidata.org/wiki/Q735","display_name":"Art","level":0,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.5534/wjmh.250110","doi":"https://doi.org/10.5534/wjmh.250110","is_oa":true,"landing_page_url":"https://doi.org/10.5534/wjmh.250110","pdf_url":"http://wjmh.org/Synapse/Data/PDFData/2074WJMH/wjmh-43-e64.pdf","source":{"id":"https://openalex.org/S2764675658","display_name":"The World Journal of Men s Health","issn_l":"2287-4208","issn":["2287-4208","2287-4690"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","kci-registered","ki-jl-1","norway-1","oarl","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319420","host_organization_name":"Korean Society for Sexual Medicine and Andrology","host_organization_lineage":["https://openalex.org/P4310319420"],"host_organization_lineage_names":["Korean Society for Sexual Medicine and Andrology"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The World Journal of Men's Health","raw_type":"journal-article"},{"id":"pmid:40878121","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/40878121","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The world journal of men's health","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13302969","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13302969/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"World J Mens Health","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:50f32256d2a8402a9a95cd612a0c10c8","doi":null,"is_oa":false,"landing_page_url":"https://doi.org/10.5534/wjmh.250110","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"The World Journal of Men's Health, Vol 44, Iss 3, Pp 669-677 (2026)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.5534/wjmh.250110","doi":"https://doi.org/10.5534/wjmh.250110","is_oa":true,"landing_page_url":"https://doi.org/10.5534/wjmh.250110","pdf_url":"http://wjmh.org/Synapse/Data/PDFData/2074WJMH/wjmh-43-e64.pdf","source":{"id":"https://openalex.org/S2764675658","display_name":"The World Journal of Men s Health","issn_l":"2287-4208","issn":["2287-4208","2287-4690"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","kci-registered","ki-jl-1","norway-1","oarl","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319420","host_organization_name":"Korean Society for Sexual Medicine and Andrology","host_organization_lineage":["https://openalex.org/P4310319420"],"host_organization_lineage_names":["Korean Society for Sexual Medicine and Andrology"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The World Journal of Men's Health","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1295591009","display_name":null,"funder_award_id":"2018R1C1B508379414","funder_id":"https://openalex.org/F4320322120","funder_display_name":"National Research Foundation of Korea"},{"id":"https://openalex.org/G5406472562","display_name":null,"funder_award_id":"20022852","funder_id":"https://openalex.org/F4320321681","funder_display_name":"Ministry of Trade, Industry and Energy"}],"funders":[{"id":"https://openalex.org/F4320320671","display_name":"National Research Foundation","ror":"https://ror.org/05s0g1g46"},{"id":"https://openalex.org/F4320321218","display_name":"Eulji University","ror":"https://ror.org/005bty106"},{"id":"https://openalex.org/F4320321681","display_name":"Ministry of Trade, Industry and Energy","ror":"https://ror.org/008nkqk13"},{"id":"https://openalex.org/F4320322120","display_name":"National Research Foundation of Korea","ror":"https://ror.org/013aysd81"},{"id":"https://openalex.org/F4320328359","display_name":"Ministry of Science and ICT, South Korea","ror":"https://ror.org/01wpjm123"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4413482014.pdf","grobid_xml":"https://content.openalex.org/works/W4413482014.grobid-xml"},"referenced_works_count":23,"referenced_works":["https://openalex.org/W61523474","https://openalex.org/W1486184828","https://openalex.org/W1975587192","https://openalex.org/W1977027676","https://openalex.org/W1992502121","https://openalex.org/W2078445138","https://openalex.org/W2116235561","https://openalex.org/W2118468892","https://openalex.org/W2119513411","https://openalex.org/W2161108332","https://openalex.org/W2274492987","https://openalex.org/W2599086938","https://openalex.org/W2736831818","https://openalex.org/W2773756092","https://openalex.org/W2910587380","https://openalex.org/W2951179095","https://openalex.org/W3016314448","https://openalex.org/W3024777192","https://openalex.org/W3146558145","https://openalex.org/W3214878742","https://openalex.org/W4285077522","https://openalex.org/W4306149473","https://openalex.org/W4362506542"],"related_works":["https://openalex.org/W2409473155","https://openalex.org/W2033469668","https://openalex.org/W61523474","https://openalex.org/W2410074883","https://openalex.org/W4401482335","https://openalex.org/W1567694907","https://openalex.org/W2359325513","https://openalex.org/W2099960525","https://openalex.org/W2752879198","https://openalex.org/W2922170633"],"abstract_inverted_index":{"PURPOSE:":[0],"To":[1,90],"develop":[2],"a":[3,46,73,191,207],"noble":[4],"on-site":[5,209,240],"dual":[6,31,210,226],"screening":[7,32,211],"system":[8,160,212,228],"for":[9,40,161,213],"sperm":[10,41,50,62,88,113,137,148,156,162,196,214],"concentration":[11],"and":[12,24,82,133,139,152,198,216,233],"hyaluronidase":[13],"(HAdase)":[14],"activity":[15,60,164],"of":[16,29,61,94,136,147,155],"male":[17,237],"fertility":[18,238],"based":[19],"on":[20,64],"lateral-flow":[21],"assay":[22,159,227],"methods,":[23,97],"to":[25],"evaluate":[26,91],"the":[27,59,83,87,92,95,98,118,140,145,169,172,174,177,179,231],"effectiveness":[28],"this":[30,224],"system.":[33],"MATERIALS":[34],"AND":[35],"METHODS:":[36],"A":[37],"chromogenic":[38],"reaction":[39,54,124],"count":[42,135,146,154,197,215],"was":[43,55,129,165,186,190,203],"established":[44],"using":[45,102],"sperm-specific":[47],"antibody":[48],"against":[49],"antigen-10":[51],"(SP-10).":[52],"Substrate-enzyme":[53],"developed":[56,96,205],"in":[57,131,150],"which":[58],"HAdase":[63,163,170,199,217],"hyaluronic":[65],"acid":[66],"(HA)":[67],"substrates":[68,79],"could":[69],"be":[70],"measured":[71],"through":[72],"color":[74,84,123,141,180],"reaction.":[75],"The":[76,158],"colored":[77],"HA":[78],"were":[80,100,115],"degraded":[81],"faded":[85],"by":[86,117,125],"HAdase.":[89],"validity":[93],"results":[99],"compared":[101],"49":[103],"semen":[104],"samples":[105],"(normozoospermia,":[106],"20;":[107],"oligozoospermia,":[108],"9;":[109],"azoospermia,":[110],"20)":[111],"whose":[112],"counts":[114],"confirmed":[116,187],"conventional":[119],"method.":[120],"RESULTS:":[121],"Using":[122],"SP-10,":[126],"no":[127],"signal":[128,142],"observed":[130],"azoospermia":[132],"low":[134],"(<16\u00d710\u2076/mL),":[138],"increased":[143,149],"as":[144],"normal":[151],"high":[153],"(\u226516\u00d710\u2076/mL).":[157],"successfully":[166,204],"developed.":[167],"In":[168],"assay,":[171],"stronger":[173],"enzyme":[175],"activity,":[176,218],"lower":[178],"signal.":[181],"Through":[182],"statistical":[183],"analysis,":[184],"it":[185],"that":[188,206,223],"there":[189],"statistically":[192],"significant":[193],"correlation":[194],"between":[195],"activity.":[200],"CONCLUSIONS:":[201],"It":[202],"novel":[208,225],"simultaneously.":[219],"We":[220],"are":[221],"expecting":[222],"will":[229],"provide":[230],"accessible":[232],"useful":[234],"information":[235],"about":[236],"at":[239],"or":[241],"home.":[242]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2025-10-10T00:00:00"}