{"id":"https://openalex.org/W4413469887","doi":"https://doi.org/10.1016/j.microb.2025.100519","title":"Functional transformation of leucine-rich repeat (LRR) polygalacturonase inhibitor protein (PGIP) of Moringa oleifera by dual mutation E146G and E218K in defense against Cercospora baticola","display_name":"Functional transformation of leucine-rich repeat (LRR) polygalacturonase inhibitor protein (PGIP) of Moringa oleifera by dual mutation E146G and E218K in defense against Cercospora baticola","publication_year":2025,"publication_date":"2025-08-23","ids":{"openalex":"https://openalex.org/W4413469887","doi":"https://doi.org/10.1016/j.microb.2025.100519"},"language":"en","primary_location":{"id":"doi:10.1016/j.microb.2025.100519","doi":"https://doi.org/10.1016/j.microb.2025.100519","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.microb.2025.100519","pdf_url":null,"source":{"id":"https://openalex.org/S4387290354","display_name":"The Microbe","issn_l":"2950-1946","issn":["2950-1946"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Microbe","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.microb.2025.100519","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Divya Chauhan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1141210","display_name":"Indian Agricultural Statistics Research Institute","ror":"https://ror.org/03kkevc75","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1141210","https://openalex.org/I179420787"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Divya Chauhan","raw_affiliation_strings":["ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India","institution_ids":["https://openalex.org/I1141210"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Santosh Kumar Behera","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210102411","display_name":"National Institute of Pharmaceutical Education and Research - Ahmedabad","ror":"https://ror.org/01dphnv87","country_code":"IN","type":"government","lineage":["https://openalex.org/I4210102411"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Santosh Kumar Behera","raw_affiliation_strings":["National Institute of Pharmaceutical Education and Research, Ahmedabad, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Pharmaceutical Education and Research, Ahmedabad, India","institution_ids":["https://openalex.org/I4210102411"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Mayank Rashmi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1141210","display_name":"Indian Agricultural Statistics Research Institute","ror":"https://ror.org/03kkevc75","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1141210","https://openalex.org/I179420787"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Mayank Rashmi","raw_affiliation_strings":["ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India","institution_ids":["https://openalex.org/I1141210"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075336087","display_name":"Mahender Kumar Singh","orcid":"https://orcid.org/0000-0001-9617-6112","observed_orcids":["https://orcid.org/0000-0001-9617-6112"]},"institutions":[{"id":"https://openalex.org/I85332549","display_name":"National Brain Research Centre","ror":"https://ror.org/022swbj46","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I85332549"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Mahender Kumar Singh","raw_affiliation_strings":["National Brain Research Center, Manesar, India"],"raw_orcid":"https://orcid.org/0000-0001-9617-6112","affiliations":[{"raw_affiliation_string":"National Brain Research Center, Manesar, India","institution_ids":["https://openalex.org/I85332549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036981091","display_name":"Raja Shankar","orcid":"https://orcid.org/0000-0002-5494-8089","observed_orcids":["https://orcid.org/0000-0002-5494-8089"]},"institutions":[{"id":"https://openalex.org/I1332470094","display_name":"Indian Institute of Horticultural Research","ror":"https://ror.org/00s2dqx11","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1332470094","https://openalex.org/I179420787"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Raja Shankar","raw_affiliation_strings":["ICAR-Indian Institute of Horticultural Research, Bengaluru, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR-Indian Institute of Horticultural Research, Bengaluru, India","institution_ids":["https://openalex.org/I1332470094"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058236540","display_name":"Girish Kumar Jha","orcid":"https://orcid.org/0000-0002-3872-4843","observed_orcids":["https://orcid.org/0000-0002-3872-4843"]},"institutions":[{"id":"https://openalex.org/I1141210","display_name":"Indian Agricultural Statistics Research Institute","ror":"https://ror.org/03kkevc75","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1141210","https://openalex.org/I179420787"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Girish Kumar Jha","raw_affiliation_strings":["ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India","institution_ids":["https://openalex.org/I1141210"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100722291","display_name":"Sunil Kumar","orcid":"https://orcid.org/0000-0001-9411-3863","observed_orcids":["https://orcid.org/0000-0001-9411-3863"]},"institutions":[{"id":"https://openalex.org/I1141210","display_name":"Indian Agricultural Statistics Research Institute","ror":"https://ror.org/03kkevc75","country_code":"IN","type":"facility","lineage":["https://openalex.org/I1141210","https://openalex.org/I179420787"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Sunil Kumar","raw_affiliation_strings":["ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India"],"raw_orcid":"https://orcid.org/0000-0001-9411-3863","affiliations":[{"raw_affiliation_string":"ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India","institution_ids":["https://openalex.org/I1141210"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5100722291"],"corresponding_institution_ids":["https://openalex.org/I1141210"],"apc_list":{"value":2150,"currency":"USD","value_usd":2150},"apc_paid":{"value":2150,"currency":"USD","value_usd":2150},"fwci":0.2273,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.34857939,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":93},"biblio":{"volume":"8","issue":null,"first_page":"100519","last_page":"100519"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.4668000042438507,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.4668000042438507,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10076","display_name":"Plant-Microbe Interactions and Immunity","score":0.16689999401569366,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.0674000009894371,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/moringa-oleifera","display_name":"Moringa oleifera","score":0.890999972820282},{"id":"https://openalex.org/keywords/polygalacturonase-inhibitor-protein","display_name":"polygalacturonase inhibitor protein","score":0.906000018119812},{"id":"https://openalex.org/keywords/polygalacturonase-inhibiting-protein","display_name":"polygalacturonase-inhibiting protein","score":0.6959999799728394},{"id":"https://openalex.org/keywords/brown-spot","display_name":"brown spot","score":0.9409999847412109},{"id":"https://openalex.org/keywords/polygalacturonase","display_name":"polygalacturonase","score":0.8569999933242798},{"id":"https://openalex.org/keywords/leucine-rich-repeats","display_name":"leucine-rich repeats","score":0.8309999704360962},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.8460000157356262},{"id":"https://openalex.org/keywords/host-pathogen-interaction","display_name":"host-pathogen interaction","score":0.8230000138282776},{"id":"https://openalex.org/keywords/protein-protein-binding","display_name":"protein-protein binding","score":0.7070000171661377},{"id":"https://openalex.org/keywords/conformational-flexibility","display_name":"conformational flexibility","score":0.8339999914169312},{"id":"https://openalex.org/keywords/plant-disease-resistance","display_name":"plant disease resistance","score":0.5699999928474426}],"concepts":[{"id":"https://openalex.org/C2776836740","wikidata":"https://www.wikidata.org/wiki/Q310468","display_name":"Moringa","level":2,"score":0.758089542388916},{"id":"https://openalex.org/C2776656883","wikidata":"https://www.wikidata.org/wiki/Q2066868","display_name":"Cercospora","level":3,"score":0.6692506074905396},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.5837626457214355},{"id":"https://openalex.org/C2776580952","wikidata":"https://www.wikidata.org/wiki/Q483745","display_name":"Leucine","level":3,"score":0.5643425583839417},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.5269259214401245},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3828410804271698},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34799396991729736},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.317036509513855},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.25042861700057983},{"id":"https://openalex.org/C2780034373","wikidata":"https://www.wikidata.org/wiki/Q6508875","display_name":"Leaf spot","level":2,"score":0.11229875683784485},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10494288802146912},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.10382631421089172},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.06487277150154114}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1016/j.microb.2025.100519","doi":"https://doi.org/10.1016/j.microb.2025.100519","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.microb.2025.100519","pdf_url":null,"source":{"id":"https://openalex.org/S4387290354","display_name":"The Microbe","issn_l":"2950-1946","issn":["2950-1946"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Microbe","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:fb0b9b8caa27435a88b0ad13d1e98786","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/fb0b9b8caa27435a88b0ad13d1e98786","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"The Microbe, Vol 8, Iss , Pp 100519- (2025)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.microb.2025.100519","doi":"https://doi.org/10.1016/j.microb.2025.100519","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.microb.2025.100519","pdf_url":null,"source":{"id":"https://openalex.org/S4387290354","display_name":"The Microbe","issn_l":"2950-1946","issn":["2950-1946"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Microbe","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.6735000014305115,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1799854541","display_name":null,"funder_award_id":"BT/PR40161/BTIS/137/32/2021","funder_id":"https://openalex.org/F4320320717","funder_display_name":"Department of Biotechnology, Ministry of Science and Technology, India"}],"funders":[{"id":"https://openalex.org/F4320320717","display_name":"Department of Biotechnology, Ministry of Science and Technology, India","ror":"https://ror.org/03tjsyq23"},{"id":"https://openalex.org/F4320320769","display_name":"Indian Council of Agricultural Research","ror":"https://ror.org/04fw54a43"},{"id":"https://openalex.org/F4320327977","display_name":"Ministry of Agriculture and Farmers Welfare","ror":"https://ror.org/01d1n9n57"},{"id":"https://openalex.org/F4320336714","display_name":"ICAR-Indian Agricultural Statistics Research Institute","ror":"https://ror.org/03kkevc75"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W1031578623","https://openalex.org/W1522033325","https://openalex.org/W1605093195","https://openalex.org/W1803102843","https://openalex.org/W1968903092","https://openalex.org/W1985629904","https://openalex.org/W1986191025","https://openalex.org/W2052038479","https://openalex.org/W2060809301","https://openalex.org/W2063947073","https://openalex.org/W2088933990","https://openalex.org/W2102993309","https://openalex.org/W2105099057","https://openalex.org/W2118571345","https://openalex.org/W2119058787","https://openalex.org/W2152375430","https://openalex.org/W2402056727","https://openalex.org/W2977578428","https://openalex.org/W3015438624","https://openalex.org/W3031104145","https://openalex.org/W3112491546","https://openalex.org/W3147425578","https://openalex.org/W3177828909","https://openalex.org/W3215286852","https://openalex.org/W3215782015","https://openalex.org/W4211196614","https://openalex.org/W4246356731","https://openalex.org/W4295165394","https://openalex.org/W4318052062","https://openalex.org/W4391165896","https://openalex.org/W4396830275","https://openalex.org/W4402563726","https://openalex.org/W4406294783","https://openalex.org/W4408230600"],"related_works":["https://openalex.org/W2144593164","https://openalex.org/W4406294783","https://openalex.org/W7131297711","https://openalex.org/W2059637327","https://openalex.org/W2084858774","https://openalex.org/W2161895024","https://openalex.org/W1605093195","https://openalex.org/W2116466301","https://openalex.org/W2105099057","https://openalex.org/W86023265"],"abstract_inverted_index":{"Cercospora":[0],"baticola,":[1],"a":[2,19,39,221,239,309],"fungal":[3,57],"phytopathogen,":[4],"is":[5],"responsible":[6],"for":[7,313],"causing":[8],"brown":[9],"spot":[10],"disease":[11,321],"in":[12,26,243,265,279,290,323],"Moringa":[13],"oleifera":[14,37,281],"by":[15,42,51,157,200,220],"secreting":[16],"polygalacturonases":[17],"(PGs),":[18],"class":[20],"of":[21,71,81,97,224,287,302],"enzymes":[22],"that":[23],"degrade":[24],"pectin":[25],"the":[27,68,72,78,91,108,174,244,266,284,300],"plant":[28],"cell":[29],"wall":[30],"to":[31,66,76,103,111,164],"facilitate":[32],"infection.":[33],"In":[34],"response,":[35],"M.":[36,280],"mounts":[38],"defense":[40],"mechanism":[41],"producing":[43],"polygalacturonase-inhibiting":[44],"proteins":[45],"(moPGIPs),":[46],"which":[47],"act":[48],"as":[49,198],"inhibitors":[50],"forming":[52],"direct":[53],"protein\u2013protein":[54],"interactions":[55],"with":[56],"PGs,":[58],"thereby":[59],"neutralizing":[60],"their":[61,105],"enzymatic":[62],"activity.This":[63],"study":[64,296],"aims":[65],"elucidate":[67],"structural":[69,217],"basis":[70],"moPGIP\u2013cbPG":[73],"interaction":[74,166,241],"and":[75,87,113,125,140,148,150,168,176,215,234,251,262,282],"evaluate":[77,165],"functional":[79,285],"consequences":[80],"two":[82],"specific":[83],"site-directed":[84,126],"mutations":[85,100,127],"E146G":[86],"E218K":[88],"located":[89],"within":[90],"conserved":[92,288],"leucine-rich":[93],"repeat":[94],"(LRR)":[95],"domains":[96],"moPGIP.":[98],"These":[99],"were":[101,118,128,134],"designed":[102],"explore":[104],"impact":[106],"on":[107],"protein\u2019s":[109],"ability":[110],"bind":[112],"inhibit":[114],"cbPG.":[115],"Protein":[116],"structures":[117],"predicted":[119],"using":[120,130,154],"multiple":[121],"homology":[122],"modeling":[123],"platforms,":[124],"introduced":[129],"PyMOL.":[131],"Structural":[132],"validations":[133],"performed":[135],"through":[136],"Ramachandran":[137],"plot":[138],"analysis":[139,172,249],"energy":[141,253],"scoring.":[142],"Rigid":[143],"docking":[144],"between":[145,173],"moPGIP":[146,178],"(wild-type":[147],"mutants)":[149],"cbPG":[151],"was":[152],"conducted":[153],"HDOCK,":[155],"followed":[156],"100":[158],"ns":[159],"molecular":[160,273,304],"dynamics":[161],"(MD)":[162],"simulations":[163],"stability":[167],"dynamic":[169],"behavior.":[170],"Comparative":[171],"wild-type":[175],"double-mutant":[177],"revealed":[179],"several":[180],"key":[181],"differences.":[182],"The":[183],"mutant":[184,245,267],"complex":[185],"exhibited":[186],"weakened":[187],"binding":[188],"affinity":[189],"(\u22120.57":[190],"kcal/mol":[191],"vs.":[192,212],"\u22120.66":[193],"kcal/mol),":[194],"increased":[195],"residue-level":[196],"flexibility":[197,261],"indicated":[199,238],"root-mean-square":[201],"fluctuations":[202],"(RMSF),":[203],"greater":[204],"conformational":[205,260],"diversity":[206],"(principal":[207],"component":[208,248],"trace:":[209],"115.9":[210],"nm\u00b2":[211],"48.4":[213],"nm\u00b2),":[214],"decreased":[216],"compactness,":[218],"reflected":[219],"broader":[222],"radius":[223],"gyration":[225],"(Rg:":[226],"2.8\u20136.0":[227],"nm).":[228],"Additionally,":[229],"solvent-accessible":[230],"surface":[231],"area":[232],"(SASA)":[233],"atomic":[235],"density":[236],"measurements":[237],"destabilized":[240],"interface":[242],"complex.":[246],"Principal":[247],"(PCA)":[250],"free":[252],"landscape":[254],"(FEL)":[255],"mapping":[256],"further":[257],"confirmed":[258],"enhanced":[259],"energetic":[263],"heterogeneity":[264],"structure.Collectively,":[268],"these":[269],"results":[270],"provide":[271],"detailed":[272],"insights":[274],"into":[275],"PGIP-mediated":[276],"resistance":[277,322],"mechanisms":[278],"highlight":[283],"relevance":[286],"residues":[289],"maintaining":[291],"stable":[292],"PG\u2013PGIP":[293],"interactions.":[294],"This":[295],"not":[297],"only":[298],"advances":[299],"understanding":[301],"host\u2013pathogen":[303],"interfaces":[305],"but":[306],"also":[307],"offers":[308],"robust":[310],"computational":[311],"framework":[312],"guiding":[314],"protein":[315],"engineering":[316],"strategies":[317],"aimed":[318],"at":[319],"enhancing":[320],"Moringa.":[324]},"counts_by_year":[{"year":2025,"cited_by_count":1}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2025-10-10T00:00:00"}