{"id":"https://openalex.org/W4413448570","doi":"https://doi.org/10.35118/apjmbb.2025.033.3.03","title":"Proof-of-concept of duplex MNAzyme assay for simultaneous detection of Candida auris and Candida spp. using synthetic DNA targets","display_name":"Proof-of-concept of duplex MNAzyme assay for simultaneous detection of Candida auris and Candida spp. using synthetic DNA targets","publication_year":2025,"publication_date":"2025-08-23","ids":{"openalex":"https://openalex.org/W4413448570","doi":"https://doi.org/10.35118/apjmbb.2025.033.3.03"},"language":"en","primary_location":{"id":"doi:10.35118/apjmbb.2025.033.3.03","doi":"https://doi.org/10.35118/apjmbb.2025.033.3.03","is_oa":true,"landing_page_url":"https://doi.org/10.35118/apjmbb.2025.033.3.03","pdf_url":"https://doi.org/10.35118/apjmbb.2025.033.3.03","source":{"id":"https://openalex.org/S176091581","display_name":"Asia-Pacific Journal of Molecular Biology and Biotechnology","issn_l":"0128-7451","issn":["0128-7451","2521-9839","2672-7277"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["jufo-1","norway-1","poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4310321046","host_organization_name":"University of Putra Malaysia","host_organization_lineage":["https://openalex.org/P4310321046"],"host_organization_lineage_names":["University of Putra Malaysia"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Asia Pacific Journal of Molecular Biology and Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.35118/apjmbb.2025.033.3.03","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001199322","display_name":"Mohd Hanif Jainlabdin","orcid":"https://orcid.org/0000-0001-9189-9469","observed_orcids":["https://orcid.org/0000-0001-9189-9469"]},"institutions":[{"id":"https://openalex.org/I4164984","display_name":"International Islamic University Malaysia","ror":"https://ror.org/03s9hs139","country_code":"MY","type":"education","lineage":["https://openalex.org/I4164984"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Mohd Hanif Jainlabdin","raw_affiliation_strings":["Institute of Planetary Survival for Sustainable Well-being (PLANETIIUM), International Islamic University Malaysia, Jalan Hospital, 25100 Kuantan, Pahang, Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Planetary Survival for Sustainable Well-being (PLANETIIUM), International Islamic University Malaysia, Jalan Hospital, 25100 Kuantan, Pahang, Malaysia","institution_ids":["https://openalex.org/I4164984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002016540","display_name":"Muhanna Mohammed Al-shaibani","orcid":"https://orcid.org/0000-0003-3776-6651","observed_orcids":["https://orcid.org/0000-0003-3776-6651"]},"institutions":[{"id":"https://openalex.org/I36197038","display_name":"Taiz University","ror":"https://ror.org/03jwcxq96","country_code":"YE","type":"education","lineage":["https://openalex.org/I36197038"]}],"countries":["YE"],"is_corresponding":false,"raw_author_name":"Muhanna Mohammed Ahmed Al-Shaibani","raw_affiliation_strings":["Faculty of Medicine and Health Sciences, Taiz University, Habeel Salman, Taiz, Yemen"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Medicine and Health Sciences, Taiz University, Habeel Salman, Taiz, Yemen","institution_ids":["https://openalex.org/I36197038"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081914315","display_name":"Mosaad Attia Abdel-Wahhab","orcid":"https://orcid.org/0000-0002-7174-3341","observed_orcids":["https://orcid.org/0000-0002-7174-3341"]},"institutions":[{"id":"https://openalex.org/I4210131959","display_name":"National Research Centre","ror":"https://ror.org/02n85j827","country_code":"EG","type":"facility","lineage":["https://openalex.org/I4210131959"]}],"countries":["EG"],"is_corresponding":false,"raw_author_name":"Mosaad Attia Abdel Wahhab","raw_affiliation_strings":["Department of Food Toxicology & Contaminants, National Research Centre, Dokki, Cairo, Egypt"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Toxicology & Contaminants, National Research Centre, Dokki, Cairo, Egypt","institution_ids":["https://openalex.org/I4210131959"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033673660","display_name":"Mohammad Ridhuan Mohd Ali","orcid":"https://orcid.org/0000-0002-5888-3526","observed_orcids":["https://orcid.org/0000-0002-5888-3526"]},"institutions":[{"id":"https://openalex.org/I4210130795","display_name":"Institute for Medical Research","ror":"https://ror.org/03bpc5f92","country_code":"MY","type":"facility","lineage":["https://openalex.org/I4210130795"]},{"id":"https://openalex.org/I4391012531","display_name":"National Institutes of Health","ror":"https://ror.org/045p44t13","country_code":"MY","type":"government","lineage":["https://openalex.org/I105292261","https://openalex.org/I4391012531"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Mohammad Ridhuan Mohd Ali","raw_affiliation_strings":["Bacteriology Unit, Infectious Disease Research Centre, Institute for Medical Research, National Institutes of Health, Jalan Setia Murni U13/52, Seksyen U13 Setia Alam, 40170 Shah Alam, Selangor, Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bacteriology Unit, Infectious Disease Research Centre, Institute for Medical Research, National Institutes of Health, Jalan Setia Murni U13/52, Seksyen U13 Setia Alam, 40170 Shah Alam, Selangor, Malaysia","institution_ids":["https://openalex.org/I4210130795","https://openalex.org/I4391012531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060322549","display_name":"Thomas Edwards","orcid":"https://orcid.org/0000-0003-4058-4461","observed_orcids":["https://orcid.org/0000-0003-4058-4461"]},"institutions":[{"id":"https://openalex.org/I194839184","display_name":"Liverpool School of Tropical Medicine","ror":"https://ror.org/03svjbs84","country_code":"GB","type":"other","lineage":["https://openalex.org/I194839184"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Thomas Edwards","raw_affiliation_strings":["The Centre for Drugs and Diagnostics, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Centre for Drugs and Diagnostics, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, United Kingdom","institution_ids":["https://openalex.org/I194839184"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5069282549","display_name":"Jorge Tovar-Torres","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I184558857","display_name":"Royal Holloway University of London","ror":"https://ror.org/04g2vpn86","country_code":"GB","type":"education","lineage":["https://openalex.org/I124357947","https://openalex.org/I184558857"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Jorge Tovar-Torres","raw_affiliation_strings":["School of Life Science and Environment, Royal Holloway, University of London, Surrey, TW20 0EX United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Life Science and Environment, Royal Holloway, University of London, Surrey, TW20 0EX United Kingdom","institution_ids":["https://openalex.org/I184558857"]}]}],"institutions":[],"countries_distinct_count":4,"institutions_distinct_count":7,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"24","last_page":"30"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10150","display_name":"Antifungal resistance and susceptibility","score":0.49050000309944153,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10150","display_name":"Antifungal resistance and susceptibility","score":0.49050000309944153,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.39719998836517334,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.02239999920129776,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/candida-auris","display_name":"Candida auris","score":0.968999981880188},{"id":"https://openalex.org/keywords/melt-curve-analysis","display_name":"melting curve analysis","score":0.656000018119812},{"id":"https://openalex.org/keywords/probe-hybridization","display_name":"probe hybridization","score":0.5630000233650208},{"id":"https://openalex.org/keywords/multidrug-resistant-fungi","display_name":"multidrug-resistant fungi","score":0.6290000081062317},{"id":"https://openalex.org/keywords/rapid-molecular-detection","display_name":"rapid molecular detection","score":0.36800000071525574}],"concepts":[{"id":"https://openalex.org/C2909786013","wikidata":"https://www.wikidata.org/wiki/Q26816853","display_name":"Candida auris","level":3,"score":0.8775656819343567},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5162270069122314},{"id":"https://openalex.org/C99611785","wikidata":"https://www.wikidata.org/wiki/Q6453233","display_name":"Duplex (building)","level":3,"score":0.465410977602005},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.42205002903938293},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4006991982460022},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.34503069519996643},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2930491268634796},{"id":"https://openalex.org/C2779548794","wikidata":"https://www.wikidata.org/wiki/Q578726","display_name":"Antifungal","level":2,"score":0.05781054496765137}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.35118/apjmbb.2025.033.3.03","doi":"https://doi.org/10.35118/apjmbb.2025.033.3.03","is_oa":true,"landing_page_url":"https://doi.org/10.35118/apjmbb.2025.033.3.03","pdf_url":"https://doi.org/10.35118/apjmbb.2025.033.3.03","source":{"id":"https://openalex.org/S176091581","display_name":"Asia-Pacific Journal of Molecular Biology and Biotechnology","issn_l":"0128-7451","issn":["0128-7451","2521-9839","2672-7277"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["jufo-1","norway-1","poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4310321046","host_organization_name":"University of Putra Malaysia","host_organization_lineage":["https://openalex.org/P4310321046"],"host_organization_lineage_names":["University of Putra Malaysia"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Asia Pacific Journal of Molecular Biology and Biotechnology","raw_type":"journal-article"},{"id":"pmh:oai:generic.eprints.org:122588","doi":null,"is_oa":false,"landing_page_url":"http://irep.iium.edu.my/122588/1/122588_%20Proof-of-concept%20of%20duplex%20MNAzyme.pdf","pdf_url":"http://irep.iium.edu.my/122588/1/122588_%20Proof-of-concept%20of%20duplex%20MNAzyme.pdf","source":{"id":"https://openalex.org/S4306400713","display_name":"The International Islamic University Malaysia Repository (The International Islamic University Malaysia)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4164984","host_organization_name":"International Islamic University Malaysia","host_organization_lineage":["https://openalex.org/I4164984"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Article"}],"best_oa_location":{"id":"doi:10.35118/apjmbb.2025.033.3.03","doi":"https://doi.org/10.35118/apjmbb.2025.033.3.03","is_oa":true,"landing_page_url":"https://doi.org/10.35118/apjmbb.2025.033.3.03","pdf_url":"https://doi.org/10.35118/apjmbb.2025.033.3.03","source":{"id":"https://openalex.org/S176091581","display_name":"Asia-Pacific Journal of Molecular Biology and Biotechnology","issn_l":"0128-7451","issn":["0128-7451","2521-9839","2672-7277"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["jufo-1","norway-1","poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4310321046","host_organization_name":"University of Putra Malaysia","host_organization_lineage":["https://openalex.org/P4310321046"],"host_organization_lineage_names":["University of Putra Malaysia"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Asia Pacific Journal of Molecular Biology and Biotechnology","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8560000061988831,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1166959005","display_name":null,"funder_award_id":"RMCG20-045-0045","funder_id":"https://openalex.org/F4320324298","funder_display_name":"International Islamic University Malaysia"},{"id":"https://openalex.org/G3955178345","display_name":null,"funder_award_id":"SPI23-180-0180","funder_id":"https://openalex.org/F4320324298","funder_display_name":"International Islamic University Malaysia"}],"funders":[{"id":"https://openalex.org/F4320324298","display_name":"International Islamic University Malaysia","ror":"https://ror.org/03s9hs139"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4413448570.pdf","grobid_xml":"https://content.openalex.org/works/W4413448570.grobid-xml"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W1974637385","https://openalex.org/W2157515463","https://openalex.org/W2165989713","https://openalex.org/W2410077926","https://openalex.org/W2414559401","https://openalex.org/W2581313795","https://openalex.org/W2809702633","https://openalex.org/W2979499669","https://openalex.org/W3022413633","https://openalex.org/W3023175438","https://openalex.org/W3156847645","https://openalex.org/W4211178015","https://openalex.org/W4225591709","https://openalex.org/W4284882161","https://openalex.org/W4289334438","https://openalex.org/W4323652397","https://openalex.org/W4378902123","https://openalex.org/W4385456163","https://openalex.org/W4389046480","https://openalex.org/W4394029371","https://openalex.org/W4396724825","https://openalex.org/W4401218014"],"related_works":["https://openalex.org/W2617273949","https://openalex.org/W3092651834","https://openalex.org/W7131808773","https://openalex.org/W2979499669","https://openalex.org/W4385840021","https://openalex.org/W7155494540","https://openalex.org/W4411608969","https://openalex.org/W3132508037","https://openalex.org/W7123399719","https://openalex.org/W4399293549"],"abstract_inverted_index":{"The":[0,98,138],"global":[1],"emergence":[2],"of":[3,39,71,91],"Candida":[4,56,108],"auris":[5,30,53,105],"as":[6],"a":[7,35,40,89,126,145,150,160],"multidrug-resistant":[8],"fungal":[9,188],"pathogen":[10],"presents":[11,34],"significant":[12],"challenges":[13],"to":[14,49,133,181],"healthcare":[15],"systems,":[16],"particularly":[17],"in":[18,125,129,186],"hospital":[19],"settings.":[20],"Rapid":[21],"and":[22,54,76,93,176],"accurate":[23],"diagnostics":[24],"are":[25],"crucial":[26],"for":[27,168],"managing":[28],"C.":[29,52,104],"infections.":[31],"This":[32,157],"study":[33],"preliminary":[36],"analytical":[37],"evaluation":[38],"duplex":[41,139],"Multicomponent":[42],"Nucleic":[43],"Acid":[44],"Enzyme":[45],"(MNAzyme)":[46],"assay":[47,99],"designed":[48],"simultaneously":[50],"detect":[51],"genus-wide":[55],"species":[57,109],"using":[58,174],"synthetic":[59,117],"DNA":[60,135],"targets.":[61],"Target":[62],"sequences":[63],"were":[64],"selected":[65],"from":[66,106],"the":[67,72],"conserved":[68],"D1/D2":[69],"region":[70],"LSU":[73],"rDNA":[74],"locus,":[75],"melting":[77,111],"temperature":[78],"analysis":[79],"confirmed":[80],"distinct":[81],"probe":[82],"hybridisation":[83],"profiles,":[84],"with":[85,165],"Substrate":[86,94],"2:PCO1":[87],"yielding":[88],"Tm":[90],"45\u00b0C":[92],"3:PCO4":[95],"at":[96],"26\u00b0C.":[97],"demonstrated":[100],"specificity":[101],"by":[102],"differentiating":[103],"other":[107],"through":[110],"curve":[112],"analysis.":[113],"In":[114],"reactions":[115],"containing":[116],"targets,":[118],"probe\u2013target":[119],"binding":[120],"disrupted":[121],"probe\u2013PCO":[122],"hybrids,":[123],"resulting":[124],"marked":[127],"reduction":[128],"melt":[130],"peaks":[131],"compared":[132],"no":[134],"controls":[136],"(NDC).":[137],"format":[140],"allows":[141],"multiplex":[142],"detection":[143],"within":[144],"single":[146],"fluorescence":[147],"channel,":[148],"offering":[149],"potential":[151,167],"advantage":[152],"over":[153],"conventional":[154],"single-target":[155],"assays.":[156],"proof-of-concept":[158],"establishes":[159],"rapid,":[161],"low-cost":[162],"diagnostic":[163],"platform":[164],"promising":[166],"future":[169],"clinical":[170,175],"application.":[171],"Further":[172],"validation":[173],"environmental":[177],"samples":[178],"is":[179],"needed":[180],"support":[182],"its":[183],"broader":[184],"utility":[185],"detecting":[187],"pathogens.":[189]},"counts_by_year":[],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2025-10-10T00:00:00"}